• 제목/요약/키워드: exposed times

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Pentachlorophenol의 노출에 의한 사람 T-임파구의 hprt 유전자에서 돌연변이 빈도 (Mutant Frequency at the hprt Locus in Human T-Cell Exposed to Pentachlorophenol)

  • 윤병수;조명행;김인규;박선영;이영순
    • Toxicological Research
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    • 제13권1_2호
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    • pp.71-78
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    • 1997
  • The mutational effects of pentachlorophenol (PCP) on the hypoxanthine phosphoribosyl transf erase (hprt) locus in human T-cell were analysed by T-cell clonal assay in vitro. Cells were exposed for 24 hours at primary culture to 0~100 ppm (W/V) PCP in dimethyl sulfoxide. Treated cells were allowed at the same time to stimulate by phytohemagglutinin (PHA) and T-cell growth factor (TCGF) and then seeded in medium containing 6-thioguanine to select for hprt-negative routants. We have also defined the optimal condition for the determination of mutant frequency. The parameters investigated include survival counting, first and second subculture for clonal efficiency plating and mutant plating. Under the optimal conditions, mutant frequencies of high dose-treated cells were significantly higher than those of non-treated or low dose cells. The results indicated a clear dose-effect relationship and showed that mutant frequency in 50 ppm PCP treated cell was 4.31$\times$$10^{-5}$ (background, 8.32$\times$$10^{-6}$). Above data strongly suggest that hprt mutation assay can be used as a biomarker for the environmental risk assessment.

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감즙 염색에 의한 레이온직물의 염색성과 기능성 (Dyeability and Functionality of Rayon Fabrics Treated with Persimmon Juice)

  • 배정숙
    • 한국의류산업학회지
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    • 제17권5호
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    • pp.828-837
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    • 2015
  • The results of the analysis of dyed rayon fabrics treated with persimmon juice by padding are as follows. Rayon fabrics were dyed repeatedly with persimmon juice by a padding mangle for the development of dyeability. The merits of padding-based dyeing were easier color reproduction (versus traditional hand dyeing) of hard to obtain colors and color fastness to light and laundering. The add on weight increased with the number of padding times, and the color of the dyed fabrics had red-yellow colors with low brightness and high chromatic colors. Properties of fabrics treated with persimmon juice were: $2^{nd}$ level of light resistance, $4^{th}-5^{th}$ perspiration fastness levels, rubbing fastness, and $3^{rd}-4^{th}$ washing fastness levels. Dyed fabrics exposed to UV light have a deep yellow-red color that increased lightness and darken when exposed to sunlight. Rayon fabrics of a treated premordant indicated strong yellow colors, especially fabrics with Fe mordant indicated greenish red-yellow colors. The direction of the weft and warp increased and improved water-repellent qualities that increased with the number of dyeing padding times and persimmon juice; however, there was less anti-crease resistance. In addition, fabrics had good antibacterial activity and deodorization.

SHCC로 단면 복구후 동결융해에 노출된 콘크리트 보의 휨 및 균열특성 (Flexural and Cracking Characteristics of Concrete Beams Exposed to Freeze-Thaw Cycles after Patch-Repaired with SHCC)

  • 윤현도;김선우;전에스더;이영오;장광수;박완신
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2009년도 춘계 학술대회 제21권1호
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    • pp.21-22
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    • 2009
  • 본 논문에서는 SHCC로 단면 복구된 콘크리트 보가 동결융해 환경에 노출되는 경우 그 휨 보강 및 균열제어 특성에 대한 실험결과를 제시한다. 본 연구에서 사용된 SHCC는 PVA 및 PE 섬유를 각각 0.75% 보강한 것이며 물결합재 비를 주요변수로 한다. 실험결과 SHCC로 휨 인장측이 단면 복구된 콘크리트의 휨내력은 동결융해의 노출 유무에 따라 각각 2.42 및 3.31배 증가되었다. 또한 휨 인장측의 균열제어 성능도 우수하게 나타났으나 이러한 성능은 동결융해에 노출됨에 따라 저하되는 특성을 보였다. 이는 시멘트 복합체와 섬유계면이 반복 동결융해에 의해 손상되었기 때문인 것으로 판단된다.

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Dual effect of Low- frequency Electromagnetic Field on Muscle Histopathology of Caspian Sea Cyprinus carpio

  • Samiee, Farzaneh;Samiee, Keivandokht
    • 한국어병학회지
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    • 제30권1호
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    • pp.51-61
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    • 2017
  • The effect of electromagnetic field on aquatic organisms has received little attention. In the current study, the effect of 50Hz electromagnetic field on muscle histopathology of Caspian Sea Cyprinus carpio, a species of economic importance, was investigated. A total of 120 healthy fish were used in this study. They were classified randomly in one of two groups as follows: Control or unexposed EMF group and experimental group with 5 different magnetic field intensities (0.1, 1, 3, 5 and 7mT) at 2 different exposure times including 30 and 60 minutes. Fish in the experimental group were exposed only once. Two weeks after exposure, dorsal muscles sectioned transversely, stained and were examined using a light microscope. Histopathologic assessments showed significant difference between control and EMF exposed groups at both 30 min. (p<0.01) and 60 min. (p<0.001) exposure times. We report for the first time that electromagnetic field in interaction with muscular tissue of Cyprinus carpio exhibits a dual effect which depends on the field intensity, and exposure time. At short exposure time (30 min.), EMF stimulates muscle growth process. At longer exposure time (60 min.), EMF can damage muscle tissue and result in muscle necrosis. More research is required to elucidate precise mechanisms involved in muscle hypertrophy and pathologic changes.

전계와 자계가 식물씨앗의 발아와 초기생장에 미치는 영향 (Effects of Electric and Magnetic Fields to Seed Germination and Its Early Growth)

  • 문재덕;권남열
    • 대한전기학회논문지:전기물성ㆍ응용부문C
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    • 제55권6호
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    • pp.328-333
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    • 2006
  • All the plants on earth live under an electric and magnetic field because the earth is a magnet and there is an electric field between the charged cloud and the ground. It has been reported that electromagnetic fields influence both the activation of ions and polarization of dipoles in living cells of seeds and plants, though the mechanism of these actions is still poorly understood. In this paper, the effects of the electric and magnetic fields and exposure times to the germination of several vegetable seeds and its early growth have been investigated experimentally to find out the feasibility of a plant factory for mass production of clean and unpolluted vegetables. The germination rate and the growth rate of some seeds under the fields exposed were analysed and compared with those of unexposed ones. It is found that the germination rate and its early growth rate of exposed seeds under the fields were accelerated about 1.1-1.4 and 1.7-2.2 times in maximum compared with those of unexposed ones. But, however, an inhibitory effect on germination and plant early growth were shown in the case of the higher electric and magnetic fields.

복합보온양생 방법이 극저온 조건하 플라이애시 치환 콘크리트의 온도이력에 미치는 영향 (The Effect of Heat Curing Methods on the Temperature History of the Fly Ash Concrete Subjected to Extremely Low Temperature)

  • 한민철;손호정
    • 한국건설순환자원학회논문집
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    • 제7권3호
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    • pp.85-90
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    • 2012
  • In this study, temperature profile of the fly ash concrete were studied in accordance with the change of heating curing method combination for the slab concrete in order to develop efficient protection method of the concrete subjected to $-20^{\circ}C$. The slab concretes with the size of $1200mm{\times}600mm{\times}200mm$ were fabricated with W/B of 50% and exposed to $-20^{\circ}C$ for 7 days. Five different combinations of heat curing methods were applied to the slab concrete specimen; two combinations of heat supplying by electrical heater and surface heat insulation material such as polyethylene film and quadrupled layer bubble sheet based on heat enclosure installment; three combinations of heating coil embedment and surface heat insulation materials such as polyethylene film, sawdust and quadrupled layer bubble sheet based on heat enclosure installment. Test results showed that by applying both heating coil and bubble sheet and heat enclosure, the concrete exposed to $-20^{\circ}C$ can be effectively protected from early-age frost damage.

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An experimental investigation on effect of elevated temperatures on bond strength between externally bonded CFRP and concrete

  • Attari, Behzad;Tavakkolizadeh, Mohammadreza
    • Steel and Composite Structures
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    • 제32권5호
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    • pp.559-569
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    • 2019
  • The bond strength between composite laminates and concrete is a key factor that controls the behavior of concrete members strengthened with fiber reinforced polymer (FRP) sheets, which can be affected by several parameters such as thermal stresses and surface preparation. This article presents the result of an experimental study on the bond strength between FRP sheets and concrete at ambient temperature after specimens had been exposed to elevated temperatures of up to $200^{\circ}C$. For this purpose, 30 specimens of plain concrete with dimensions of $150{\times}150{\times}350mm$ were prepared. Three different conventional surface preparation methods (sandblasting, wire brushing and hole drilling) were considered and compared with a new efficient method (fiber implantation). Deformation field during each experiment was monitored using particle image velocimetry. The results showed that, the specimens which were prepared by conventional surface preparation methods, preserved their bond integrity when exposed to temperature below glass transition temperature of epoxy resin (about $60^{\circ}C$). Beyond this temperature, the bond strength and stiffness decreased significantly (about 50%) in comparison with control specimens. However, the specimens prepared by the proposed method displayed higher bond strengths of up to 32% and 90% at $25^{\circ}C$ and $200^{\circ}C$, respectively.

36개월간 국내 옥외폭로시험에 따른 아연도강의 표면외관 변화 (Surface Appearance of Galvanized Steel with Outdoor Exposure Testing in Korea for 36 Months)

  • 김기태;김영식
    • Corrosion Science and Technology
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    • 제17권6호
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    • pp.301-309
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    • 2018
  • If galvanized steel is exposed to an outdoor environment, atmospheric corrosion will occur with time and red rust will form when the sacrificial protection capacity of zinc reaches its limit. With corrosion, the surface appearance of steel changes, and the properties of the exterior materials degrade. In this study, two kinds of galvanized steel, (GA and GI specimens) were subjected to an outdoor exposure test for 36 months in six regions of Korea. Chrominance (color, chroma, and brightness) and glossiness surface analyses were performed. The color change was not significant, regardless of the exposed area or the specimens tested. With increasing exposure times, the GA specimen became blackened by the formation of zinc oxide, and red coloration was increased by the formation of red rust. As the exposure time of GI specimen increased, the surface proceeded to blacken, but no red rust was formed and the color did not change significantly. Regardless of the outdoor exposure area or the specimen, longer exposure times led to lower glossiness, and this behavior appears to be influenced by the formation of zinc oxide.

Whole-Body Vibration Exposure vis-à-vis Musculoskeletal Health Risk of Dumper Operators Compared to a Control Group in Coal Mines

  • Kumar, Vivekanand;Palei, Sanjay K.;Karmakar, Netai C.;Chaudhary, Dhanjee K.
    • Safety and Health at Work
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    • 제13권1호
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    • pp.73-77
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    • 2022
  • Background: Whole-body vibration (WBV) exposure of coal mine dumper operators poses numerous health hazards. The case-control study was aimed at assessing the relative musculoskeletal health risk of dumper operators' exposure to WBV with reference to the nonexposed group. Methods: Measurements of WBV exposure were taken at the operator-seat interface using a human vibration analyzer for 110 dumper operators in three coal mines. This vibration measurement was supplemented by a questionnaire survey of 110 dumper operators exposed to WBV and an equal number of workers not exposed to WBV. The relative risk of musculoskeletal disorders (MSDs) has been assessed through the case-control study design. Results: ISO guidelines were used to compare the health risk. It was observed that the prevalence of pain in the lower back was 2.52 times more in the case group compared to the control group. The case group of Mine-2 was 2.0 times more prone to vibration hazards as compared to Mine-3. Conclusion: The case group is more vulnerable to MSDs than the control group. The on-site measurement as well as the response of the dumper operators during the questionnaire survey corroborates this finding.

Ultra-sensitive Determination of Salinomycin in Serum Using ICP-MS with Nanoparticles

  • Cho, H.K.;Lim, H.B.
    • Bulletin of the Korean Chemical Society
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    • 제35권11호
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    • pp.3195-3198
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    • 2014
  • An ultra-sensitive detection method for small molecules such as antibiotics was developed using ICP-MS with magnetic and $TiO_2$ nanoparticles. Since most of the antibiotics are too small to employ a sandwich-type extraction through an immunoreaction, a non-specific platform was employed, in which the target was extracted by magnetic separation, followed by tagging with $TiO_2$ nanoparticles of 11.2 nm for ICP-MS measurement. The detection limit for salinomycin obtained from spiked serum samples was $0.4ag\;mL^{-1}$ (${\pm}10.3%$), which was about $1.5{\times}10^6$ times lower than that of LC-MS/MS and about $1.2{\times}10^{11}$ times better than that of ELISA. Such an excellent sensitivity enabled us to study the toxicity of antibiotics exposed to human beings by determining them in serum.