Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지

Mutant Frequency at the hprt Locus in Human T-Cell Exposed to Pentachlorophenol

Pentachlorophenol의 노출에 의한 사람 T-임파구의 hprt 유전자에서 돌연변이 빈도

  • 윤병수 (경기대학교 생물학과) ;
  • 조명행 (서울대학교 수의과대학) ;
  • 김인규 (한국원자력연구소 방사선생체해석분야) ;
  • 박선영 (한국원자력연구소 방사선생체해석분야) ;
  • 이영순 (서울대학교 수의과대학)
  • Published : 1997.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The mutational effects of pentachlorophenol (PCP) on the hypoxanthine phosphoribosyl transf erase (hprt) locus in human T-cell were analysed by T-cell clonal assay in vitro. Cells were exposed for 24 hours at primary culture to 0~100 ppm (W/V) PCP in dimethyl sulfoxide. Treated cells were allowed at the same time to stimulate by phytohemagglutinin (PHA) and T-cell growth factor (TCGF) and then seeded in medium containing 6-thioguanine to select for hprt-negative routants. We have also defined the optimal condition for the determination of mutant frequency. The parameters investigated include survival counting, first and second subculture for clonal efficiency plating and mutant plating. Under the optimal conditions, mutant frequencies of high dose-treated cells were significantly higher than those of non-treated or low dose cells. The results indicated a clear dose-effect relationship and showed that mutant frequency in 50 ppm PCP treated cell was 4.31$\times$$10^{-5}$ (background, 8.32$\times$$10^{-6}$). Above data strongly suggest that hprt mutation assay can be used as a biomarker for the environmental risk assessment.

Keywords

References

  1. Nature v.316 Alterations of the hprt gene in human in vivo-derived 6-thioguanine resistant T lymphocytes Albertini, R.J.;O'Neill, J.P.;Nicklas, J.A.;Heintz, N.H.;Kelleher, P.C.
  2. J. Immunol. v.123 Human T-cell growth factor. 1. Optimal conditions for its production Alvarez, J.M.;Silva, A.;de Landazuri, M.O.
  3. Cancer Research v.52 In vitro mutational specificity of cisplatin in the human hypoxanthine guanine phosphoribosyltransferase gene Cariello, N.F.;Swenberg, J.A.;Skopek, T.R.
  4. Antiviral chemotherapy: Design of inhibitors of viral functions Effect of chemical carcinogens and mutagens on the transformation of mammalian cells by DNA virus Casto, B.C.;Gauri, K.K.(Ed.)
  5. Computer-environmental Legislative Data Systems CELDS
  6. Pentachlorophenol, chemistry, pharmacology and environmental toxicology Patterns of pentachlorophenol usage in America. - an overview Cirelli, D.P.;Rao, K.R.(Ed.)
  7. Arch. Environ. Contam. Toxicol. v.18 Pentachlorophenol measurements in body fluids of people in long homes and workplace Cline, R.E.;Hill, R.H.J.;Phillips, D.L.
  8. Carcinogenesis Mutagenicity of butadiene and its epoxide metabolites:Ⅰ. Mutagenic potential of 1,2,-epoxybutene, 1,2,3,4,-diepoxybutane, and 3,4,-epoxy-1,2,-butanediol in cultured human lymphoblasts Cochrane, J.E.;Skopek, T.R.
  9. Mutation Reserch v.273 Elevated hprt mutant frequency in circulating T-lymphocytes of Xeroderma pigmentosum patients Cole, J.;Arlett, C.F.;Norris, P.G.;Stephans, G.;Waugh, A.P.W.;Beare, D.M.;Green, M.H.L.
  10. Pure. Appl. Chem. v.53 Environmental chemistry of pentachlorophenol Crosby, D.G.;Beynon, K.I.;Greve, P.A.;Korte, F.;Stilli, G.G.;Vouk, J.W.
  11. Environ. Molecul. Mutagen. v.10 no.SUP.10 Chromosome abberrations and sister chromatid exchanges in Chinese hamster ovary cells; Evaluations of 108 chemicals Galloway, S.M.;Armstrong, M.J.;Reuben, C.;Coleman, S.;Brown, B.;Cannon, J.;Bloon, A.D.;Nakamura, F.;Ahmed, M.;Duk, S.;Rimpo, J.;Margolin, B.H.;Resnick, M.A.;Anderson, B.;Zeiger, E.
  12. Arch. of Environ. Contam. Toxicol. v.18 Pentachlorophenol:Environmental partitioning and human exposure Hattenman-Frey, H.A.;Travis, C.C.
  13. Arch. of Environ. Contam. Toxicol. v.18 Residues of chlorinated phenols and phenoxy acid herbicides in the urine of Akansas children Hill, R.H.;Teresa, T.;Holler, J.S.;Douglas, M.F.;Smith, S.J.;Needham, L.L.;Binder, S.
  14. International Programm on Chemical Safety: Pentachlorophenol, environmental health criteria. 71 IPCS
  15. Neurotoxicol. v.12 Pentachlorophenol concentrations in human cerebrospinal fluid Jorens, P.G.;Janssens, J.J.;Tichelen, W.I.van.
  16. Health Phy. v.57 Mutant frequency of radiotherapy technicians appears to be asociated with recent dose of ionizing radiation Messing, K.;Ferraris, J.;Bradley, W.E.C.;Swarty, J.;Seifert, A.M.
  17. Mutagenesis. v.5 Molecular analysis of in vivo hprt mutations in human T-lymphocytes. Ⅴ. Effects of total body irradiation secondary to radioimmunoglobulin therapy (RIT) Nicklas, J.A.;Falta, M.T.;Hunter, T.C.;O'Neill, J.P.;Jacobson-Kram, D.;Williams, J.R.;Albertini, R.J.
  18. Aichi Ika Daigaku Igakukai Zasshi v.10 Survey on mutagenecity of pesticides by the Salmonella-Microsome Test Nishimura, N.;Nishimura, H.;Oshima, H.
  19. Mutagenesis. v.2 Refinement of a T-lymphocyte cloning assay to quantify the in vivo thioquanine-resistant mutant frequency in humans O'Neil, J.P.;McGinniss, M.J.;Berman, J.K.;Sullivan, L.M.;Nicklas, J.A.;Albertini, R.J.
  20. Mutation Research v.240 In vitro induction, expression and selection of thioguanine-resistant mutants with human T-lymphocytes O'Neill, J.P.;Sullivan, L.M.;Albertini, R.J.
  21. Pentachlorophenol, chemistry, pharmacology and environmental toxicology Results of two-year toxicity and reproduction studies on pentachlorophenol in rats Schwetz, B.A.;Quast, I.F.;Keeler, P.A.;Huminston, C.G.;Kociba, R.J.;Rao, K.R.(Ed.)
  22. Biochem. Biophysic. Res. Com. v.84 Isolation of a human lymphoblastoid line heterozygous at the tk locus Skopek, T.R.
  23. Mutation. Research v.257 Pentachlorophenol Seiler, J.P.
  24. Mutation Research v.253 Use of the clonal assay for the measurement of frequencies of HPRT mutants in T-lymphocytes from five control populations Tates, A.D.;Dam, F.J.V.;Mossel, H.v.;Schoemaker, H.;Thijssen, J.C.P.;Woldring, V.M.;Zwindermann, A.H.;Natarajan, A.T.
  25. Toxicological profile of pentachlorophenol US DHHS
  26. Land disposal restrictions, Code of Federal Regulations, 40 CFR 268 US EPA
  27. Reviews of the environmental effects of pollutants; XI.Chlorophenols US EPA