• Title/Summary/Keyword: expanded method

Search Result 1,674, Processing Time 0.025 seconds

Comparative Evaluation of Three Purification Methods for the Nucleocapsid Protein of Newcastle Disease Virus from Escherichia coli Homogenates

  • Tan Yan Peng;Ling Tau Chuan;Yusoff Khatijah;Tan Wen Siang;Tey Beng Ti
    • Journal of Microbiology
    • /
    • v.43 no.3
    • /
    • pp.295-300
    • /
    • 2005
  • In the present study, the performances of conventional purification methods, packed bed adsorption (PBA), and expanded bed adsorption (EBA) for the purification of the nucleocapsid protein (NP) of Newcastle disease virus (NDV) from Escherichia coli homogenates were evaluated. The conventional methods for the recovery of NP proteins involved multiple steps, such as centrifugation, precipitation, dialysis, and sucrose gradient ultracentrifugation. For the PBA, clarified feedstock was used for column loading, while in EBA, unclarified feedstock was used. Streamline chelating immobilized with $Ni^{2+}$ ion was used as an affinity ligand for both PBA and EBA. The final protein yield obtained in conventional and PBA methods was $1.26\%$ and $5.56\%$, respectively. It was demonstrated that EBA achieved the highest final protein yield of $9.6\%$ with a purification factor of 7. Additionally, the total processing time of the EBA process has been shortened by 8 times compared to that of the conventional method.

Implementation of Network Address Translator (네트워크 주소변환 장치 구현)

  • Park Byoung-soo;Cho Tae-kyung
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.5 no.6
    • /
    • pp.526-532
    • /
    • 2004
  • The insufficiency on IP address cause to develope a new internet protocol, IPv6 that the length of address field is expanded. But there are actually many problems on applying and operating this standard for internet. Though NAT(Network Address Translation) is instead of it, NAT has the characteristics that is not allowed to access from outside. This is a big merit in security but a week point because the access from outside should be allowed when a small organization operate web sever or mail server. Therefore, this paper proposes the expanded NAT which can solve such problems as modifying the table of NAT. Furthermore, the function of existing VPN(Virtual Private Network) will be acceptable partly through such a method that provide the linkage among VPNs.

  • PDF

Improvement of Method for Supplying the Nutrient Solution at Expanded Rice Hull Substrates during Hydrophonic Culture of Tomato Plants (토마토 양액재배시 팽연화 왕겨 배지에 적합한 급액방법 개선)

  • 김경희;임상현;김성일;유근창
    • Journal of Bio-Environment Control
    • /
    • v.10 no.2
    • /
    • pp.101-105
    • /
    • 2001
  • Plant roots are affected by the root zone environment rather than substrate material itself. It is important to provide a suitable environment for the roots by amending the substrate and adjusting supply of the nutrient solution. In an expanded rice hull substrates, 1.5 L nutrient solution was supplied on each day at different frequency. In rice hull substrate, plant growth and yield were the greatest in the treatment where a 1.5L nutrient solution was supplied as 24 equal aliquots, wheres in perlite substrate plant growth and yield were the greatest in the treatment with 16 aliquots. Nitrogen deficiency symptoms caused by early decomposition of rice hulls by microorganisms was recovered by increasing solution EC from 1.7 to EC 2.0 dS.m$^{-1}$ for 25 days after planting. Plant growth and yield increased in the treatment of Ec 3.0 dS.m$^{-1}$ , but the cause for this increase is not clear.

  • PDF

Verification Techniques of the Distored iBeacon Information for Reliable Indoor Positioning Systems (신뢰성 있는 실내 위치 측위 시스템을 위한 왜곡된 iBeacon 정보의 검증 기법)

  • Yoon, Chang-Pyo;Hwang, Chi-Gon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
    • /
    • 2016.05a
    • /
    • pp.345-347
    • /
    • 2016
  • Recently location based services is being expanded into the indoor service that can not access to the outdoor location informations, such as GPS. Thus, the research and development of an indoor positioning system with BLE(Bluetooth Low Energy) iBeacon technology has expanded. However, RSSI (Received Signal Strength Indicator) that is used as the distance information between the terminal and for positioning iBeacon signal has a problem in that distortion occurs, information such as the signal attenuation and the delay due to the characteristics of radio waves. In this paper, we propose a reliable method of verifying iBeacon signal with the signal distortion problems for reliable indoor positioning systems.

  • PDF

An Experimental Study of the Wall Temperature of the Supersonic Impinging Coaxial Jet Using an FLIR (적외선 카메라를 이용한 초음속 충돌 동축제트의 벽면 온도 측정)

  • Gwak, Jong-Ho;Kumar, V. R. Sanal;Kim, Heuy-Dong
    • Proceedings of the KSME Conference
    • /
    • 2004.04a
    • /
    • pp.1631-1636
    • /
    • 2004
  • The supersonic impinging jet has been extensively applied to rocket launching system, gas jet cutting control, gas turbine blade cooling, etc. In such applications, wall temperature of an object on which supersonic jet impinges is a very important factor to determine the performance and life of the device. However, wall temperature data of supersonic impinging jets are not enough to data. The present study describes an experimental work to measure the wall temperatures of a vertical flat plate on which supersonic, dual, coaxial jet impinges. An Infrared camera is employed to measure the wall temperature distribution on the impinging plate. The pressure ratio of the jet is varied to obtain the supersonic jets in the range of over-expanded to moderately under-expanded conditions at the exit of coaxial nozzle. The distance between the coaxial nozzle and the flat plate was also varied. The coaxial jet flows are visualized using a Shadow optical method. The results show that the wall temperature distribution of the impinging plate is strongly dependent on the jet pressure ratio and the distance between the nozzle and plate.

  • PDF

Analysis on E-polarized Scattering by a Conducting Strip grating with 2 Dielectric Layers (2개의 유전체 층을 갖는 도체 격자구조에 의한 E-분극 산란해석)

  • Seung-In Yang
    • The Proceeding of the Korean Institute of Electromagnetic Engineering and Science
    • /
    • v.6 no.3
    • /
    • pp.68-74
    • /
    • 1995
  • In this paper, E-polarized electromagnetic scattering by a conducting strip grating with 2 dielectric layers is analyzed to calculate the geometrical reflected and transmitted power by applying the Fourier-Galerkin moment mothed. The induced current density is expanded in a Fourier series using a simple exponential func- tion, and we applied the boundary conditions to the electromagnetic fields at the boundary planes. The sca- ttered electromagnetic fields are expanded in a series of Floquet mode functions. To examine the accuracy of the present method, the geometrically reflected and transmitted power are evaluated and compared with those of the existing papers, and then the numerical results are found in good agreement with those of the existing paper.

  • PDF

Construction of Korean Knowledge Base Based on Machine Learning from Wikipedia (위키백과로부터 기계학습 기반 한국어 지식베이스 구축)

  • Jeong, Seok-won;Choi, Maengsik;Kim, Harksoo
    • Journal of KIISE
    • /
    • v.42 no.8
    • /
    • pp.1065-1070
    • /
    • 2015
  • The performance of many natural language processing applications depends on the knowledge base as a major resource. WordNet, YAGO, Cyc, and BabelNet have been extensively used as knowledge bases in English. In this paper, we propose a method to construct a YAGO-style knowledge base automatically for Korean (hereafter, K-YAGO) from Wikipedia and YAGO. The proposed system constructs an initial K-YAGO simply by matching YAGO to info-boxes in Wikipedia. Then, the initial K-YAGO is expanded through the use of a machine learning technique. Experiments with the initial K-YAGO shows that the proposed system has a precision of 0.9642. In the experiments with the expanded part of K-YAGO, an accuracy of 0.9468 was achieved with an average macro F1-measure of 0.7596.

ANSYS®-Based Gear Stress Analysis of a Lightweight 3-DOF Wrist Mechanism for a Parallel Robot with Expanded Workspace (고속 확장된 작업공간을 가진 병렬 로봇을 위한 경량 3-DOF 손목 메커니즘의 ANSYS 기반 응력해석)

  • Park, Sang Hyeok;Chung, Won Jee;Hwang, Hui Geon;Kim, Hong Rok;Choi, Se Woong;Jee, Myeong Jun;Hong, Woo Cheol
    • Journal of the Korean Society of Manufacturing Process Engineers
    • /
    • v.21 no.2
    • /
    • pp.116-122
    • /
    • 2022
  • This study proposed a method of testing the stability when selecting gears to reduce the weight of a 3-DOF wrist mechanism for a pick-and-place 3-DOF parallel robot with an increased workspace by using an additional straight axis at its top. We performed SolidWorks® modeling- and ANSYS®-based structural analysis of a pinion gear, which is most vulnerable to the force from a 3-DOF wrist mechanism, to lighten the robot weight for performing various tasks. When the initial analysis results considerably differed from the theoretical values calculated in advance, we checked and identified the errors in the contact conditions or input values. Ultimately, it is believed that the methodology presented in this paper will help in mitigating errors during analysis and determine the accurate values for a lightweight 3-DOF wrist mechanism for a parallel robot with an expanded workspace.

A Wide-field-of-view Table-ornament Display Using Electronic Holography

  • Daerak Heo;Hosung Jeon;Sungjin Lim;Joonku Hahn
    • Current Optics and Photonics
    • /
    • v.7 no.2
    • /
    • pp.183-190
    • /
    • 2023
  • Three-dimensional (3D) displays provide a significant advantage over traditional 2D displays by offering realistic images, and table-style displays in particular are ideal for generating 3D images that appear to float above a table. These systems are based on multiview displays, and are typically operated using temporal or spatial multiplexing methods to expand the viewing zone (VZ). The VZ is an expanded space that results from merging the sub-viewing zones (SVZs) from which an individual view is made. To increase the viewing angle, many SVZs are usually required. In this paper, we propose a table-ornament electronic holographic display that utilizes 3f parabolic mirrors. In holography, the VZ is not simply expanded but synthesized from SVZs to implement continuous motion parallax. Our proposed system is small enough to be applied as a table ornament, in contrast to traditional tabletop displays that are large and not easily portable. By combining multiview and holographic technologies, our system achieves continuous motion parallax. Specifically, our system projects 340 views using a time-multiplexing method over a range of 240 degrees.

Study on Emerging Security Threats and National Response

  • Il Soo Bae;Hee Tae Jeong
    • International Journal of Advanced Culture Technology
    • /
    • v.11 no.4
    • /
    • pp.34-41
    • /
    • 2023
  • The purpose of this paper is to consider the expansion of non-traditional security threats and the national-level response to the emergence of emerging security threats in ultra-uncertain VUCA situations. As a major research method for better analysis, the theoretical approach was referred to papers published in books and academic journals, and technical and current affairs data were studied through the Internet and literature research. The instability and uncertainty of the international order and security environment in the 21st century brought about a change in the security paradigm. Human security emerged as the protection target of security was expanded to individual humans, and emerging security was emerging as the security area expanded. Emerging security threatsthat have different characteristicsfrom traditionalsecurity threats are expressed in various ways, such as cyber threats, new infectious disease threats, terrorist threats, and abnormal climate threats. First, the policy and strategic response to respond to emerging security threats is integrated national crisis management based on artificial intelligence applying the concept of Foresight. Second, it is to establish network-based national crisis management smart governance. Third, it is to maintain the agile resilience of the concept of Agilience. Fourth, an integrated response system that integrates national power elements and national defense elements should be established.