• Toxicological Research
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• 제31권2호
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• pp.165-172
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• 2015

Cornus officinalis is widely distributed in Korea, and its fruit has been used to make as herbal drug for traditional medicine in Korea, Japan, and China because of its tonic, analgesic, and diuretic properties. However, the effects of C. officinalis methanol extract (COME) on melanogenesis remain poorly understood. We evaluated the melanogenic capability of COME in melan-a cells, which are immortalized mouse melanocytes. COME increased melanin synthesis in a dose-dependent manner. Treatment with $12.5{\mu}g/mL$ of COME significantly increased melanin content by 36.1% (p < 0.001) to a level even higher than that (31.6%) of 3-isobutyl-1-methyl-xanthine, a well-known pigmentation agent. COME also upregulated tyrosinase activity and its messenger RNA and protein expression. In addition, COME upregulated the expression of tyrosinase-related proteins 1 and 2 and microphthalmia-associated transcription factor-M messenger RNA expression. These results imply that COME may be appropriate for development as a natural product to treat hair graying.

• Journal of Microbiology and Biotechnology
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• 제15권2호
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• pp.425-427
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• 2005

The steam distillate obtained from Thujopsis dolabrata var. hondai sawdust was fractionated by centrifugal thin-film evaporation, and the fractions were then investigated for antiplatelet activity using washed rabbit platelets. The biologically active constituent of T. dolabrata var. hondai sawdust was isolated by silica gel column and HPLC chromatographies and characterized as carvacrol by various spectral analyses. Carvacrol inhibited platelet aggregation induced by collagen, arachidonic acid, and platelet activating factor with IC$_{50}$ values of 12.6, 2.5, and 385.3 $\mu$M, respectively. However, carvacrol had no effect on thrombin, calcium ionophore A23l87, or phorbol l2-myristate l3-acetate induced platelet aggregation. Carvacrol was a much more potent inhibitor, as antiplatelet agents, compared with aspirin. These results suggest that carvacrol isolated from T. dolabrata var. hondai sawdust may be useful as a lead compound for inhibiting arachidonic acid-induced platelet aggregation.

• 방사선산업학회지
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• 제17권3호
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• pp.219-224
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• 2023

A high-efficiency underwater radiation monitoring system, HydroGamma, has been developed for detecting ¹³⁷Cs and ¹³¹I in the event of waterborne radiation contamination. The system consists of a 3-inch NaI (Tl) detector, solar panels for power supply, data acquisition and transmission modules, and batteries. HydroGamma also includes a ⁴⁰K calibration source for remote performance evaluation and energy calibration. In this study, some simulations and experiments were carried out to evaluate the minimum detectable activities (MDA) of HydroGamma. We installed the HydroGamma at Tapjeongho Lake in Nonsan-si and acquired background data since MDA is calculated based on the experimental background data. The results show that the minimum detectable activities for ¹³⁷Cs and ¹³¹I were 1.78Bq L^-1 and 1.81Bq L^-1, respectively even though the gamma rays emitted from ⁴⁰K(1,460 keV) affect the minimum detectable activities for them.

• Toxicological Research
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• 제24권1호
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• pp.17-22
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• 2008

Sopungsan (SS) is a traditional Korean decoction used for the treatment of dermatitis. The aim of this study is to confirm whether or not SS has a preventive effect on the development of atopic dermatitis in dinitrochlorobenzene-applied Nc/Nga mice. SS was administered orally to Nc/Nga mice, which led to the remarkable suppression of the development of dermatitis, as determined by a histological examination and the serum IgE levels. Moreover, SS inhibited the production of thymus- and activation-regulated chemokine (TARC) and its mRNA expression in a keratinocyte cell line, HaCaT, which had been stimulated with tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interferon-${\gamma}$ (IFN-${\gamma}$). Activation of the nuclear factor-${\kappa}B$ (NF-${\kappa}B$) or activator protein-1 (AP-1) is one of key steps in the signaling pathways mediating induction of TARC. In this study, SS selectively suppressed NF-${\kappa}B$ activation which may be essential for TARC expression in $TNF-{\alpha}/IFN-{\gamma}$ treated keratinocytes. The inhibitory effect of SS on NF-${\kappa}B$ activation and TARC production might be associated with the anti-dermatitic effects of SS.

• Toxicological Research
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• 제24권3호
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• pp.169-174
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• 2008

This study examined the mechanisms underlying the effects of the vasorelaxation active substance(VAS), dimethyladenosine-5'-L-arabinose, and its partial purification fraction on nitric oxide synthase in improving erectile dysfunction with particular focus on the nitric oxide (NO)-cGMP pathways. Two rat models, 9-month-old SD rats and 11-month-old SD rats, were given VAS(40 mg/kg per day) for 4 days, The aqueous fraction of silworm male pupae extract; semi-purified VAS(100 mg/kg per day) for 10 days, respectively. The NOS activities of the following three enzymes were examined: neuronal NO synthase(nNOS), inducible NOS(iNOS), endothelial NOS(eNOS), vascular endothelial growth factor on endothelial cells(VEGF) and anti-inflammation effect of Tumor necrosis factor-$\alpha$. The results showed increases in the nitric oxide synthase activities. Western blotting of the tissue homogenate showed an increase in the nNOS level in the brain and tongue, and an increase in the endothelial NO synthase(eNOS) level in penis. However, there was little association with VEGF production in HUVEC endothelial cells and no relationship with TNF-$\alpha$ which showed low levels.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 제39회 하계학술대회
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• pp.1326-1327
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• 2008

Recently, the air pollution is supposed is to be the most serious environmental problem. Therefore, a small air cleaner is asked to make comfortable indoor environment for the moderns who have frequent indoor activity. In this study, without generating of the second pollution source, dust in the air and harmful microorganisms can be removed by the electric dust collecting technology which has high reliability and efficiency to be able to remove the impurities even though it is just 0.01${\mu}m$. Accordingly, power sources of the dust collector use, it is not inefficiency and offering to dust collector unstable. In order to overcome the point at issue, a this study, for power factor compensating use the PFC circuit, for removing the noise use EMI filter and for generating the hight voltage use the Cockcroft Walton Circuit. And we study power source on characteristic and improvement.

• The Korean Journal of Physiology and Pharmacology
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• 제3권1호
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• pp.69-74
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• 1999

Both direct and indirect environmental stress to brain were increase the expression of transcription factor c-fos in various populations of neurons. In this study, we examined whether the intraperitoneal injections of lidocaine at doses inducing convulsion within 10 min increased the level of c-fos mRNA and protein in forebrain areas. In situ hybridization using $[^{35}S]UTP-labeled$ antisense c-fos, cRNA increased c-fos mRNA levels though hippocampal formation, piriform cortex, septum, caudate-putamen, neostriatum, and amygdala within 2 hr. In parallel with the mRNA expression, c-FOS protein immunoreactivity was also observed in the same forebrain areas. In contrast to the seizure activity and widespread neuronal degeneration following a kainate treatment, injections of lidocaine did not produce neuronal death within 3 days. The present study indicates that lidocaine induces convulsion and c-fos expression without causing neurotoxicity.

• 기본간호학회지
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• 제13권2호
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• pp.200-207
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• 2006

Purpose: This study was done to identify factors influencing falls in inpatients. A comprehensive analysis of individual, disease, and environment related factors and an assessment of being in a risk factor group were included in the data. Method: The medical records of 325 inpatients were reviewed. Data were collected from January to July 2003. Frequencies, 1-test and $X^2-test$ were used to analyze the data and the SPSS program was used. Results: Individual-related factors for inpatients falls were age, drinking and weakness. Disease-related factors for inpatients falls were diagnostic department, admission from ER or OPD, admission by wheelchair or orther method and activity status. Environment-related factors for inpatient falls were re-education and side-rails. Assessment of the risk factor group related factors were age, history of falls, body balance, depression, communication, medication, chronic disease, urinary condition and total score for risk factors. Conclusion: Experienceing a fall among Korean inpatients was associated not only with individual factors, but also with disease and environmental factors. The findings of this study suggest that broad intervention programs should be provided to prevent inpatient falls.

• Molecular & Cellular Toxicology
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• 제5권3호
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• pp.230-236
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• 2009

Acrolein, a known toxin in cigarette smoke, is the most abundant electrophilic $\alpha$, $\beta$-unsaturated aldehyde to which humans are exposed in a variety of environmental pollutants, and is also product of lipid peroxidation. Increased unsaturated aldehyde levels and reduced antioxidant status plays a major role in the pathogenesis of various diseases such as diabetes, Alzheimer's and atherosclerosis. The findings reported here show that low concentrations of acrolein induce heme oxygenase-1 (HO-1) expression in RAW 264.7 macrophages. HO-1 induction by acrolein and signal pathways was measured using reverse transcription-polymerase chain reaction, Western blot and immunofluorescence staining analyses. Inhibition of extracellular signal-regulated kinase activity significantly attenuated the induction of HO-1 protein by acrolein, while suppression of Jun N-terminal kinase and p38 activity did not affect induction of HO-1 expression. Moreover, rottlerin, an inhibitor of protein kinase $\delta$, suppressed the upregulation of HO-1 protein production, possibly involving the interaction of NF-E2-related factor 2 (Nrf2), which has a key role as a HO-1 transcription factor. Acrolein elevated the nuclear translocation of Nrf2 in nuclear extraction. The results suggest that RAW 264.7 may protect against acrolein-mediated cellular damage via the upregulation of HO-1, which is an adaptive response to oxidative stress.

• KSBB Journal
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• 제19권1호
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• pp.1-11
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• 2004

클로렐라는 생물화학적 성분들을 풍부하게 함유하고 있어서 영양학적으로 사료나 식품으로 이용되며 화장품, 의약품, 심지어 연료로도 이용되고 있다. 클로렐라는 보통 연못이나 호수 등 담수에서 생육하며, 구형 단세포이다. 생식은 무성생식으로 하루에 4-16배로 증식한다. 클로렐라의 대량생산을 위한 배양은 크게 옥외배양법과 발효조 배양법으로 나뉜다. 클로렐라는 필수 아미노산과 생체에 여러 가지 생리적 기능을 수행하는 지방산과 스테롤, 유산균 성장 촉진 원인 물질인 chlorella growth factor (CGF)의 함유되어 있으며, 생체 내에서 중금속 배출기능, 독성물질의 분해, 동맥경화 및 간장장애의 억제, 면역기능 강화, 항암 활성, 장내 유효세균의 증식 촉진, 식품의 풍미 향상 및 보습효과 등의 기능성을 가지고 있어 기능성 생물소재로 주목되고 있다. 클로렐라는 건강기능식품, 화장품, 의약품 소재로서 응용될 수 있을 뿐만 아니라 이산화탄소의 고정화와 수소가스의 생성 등 환경 및 에너지의 생산을 위한 수단으로서의 역할이 전망된다.