Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지
DOI QR코드

DOI QR Code

Elevation of Nitric Oxide Synthase Activity by Dimethyladenosine from Silkworm Pupae in Aged Rats

  • Ahn, Mi-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Han, Jea-Woong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Hong, Yoo-Na (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Hwang, Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
  • Published : 2008.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study examined the mechanisms underlying the effects of the vasorelaxation active substance(VAS), dimethyladenosine-5'-L-arabinose, and its partial purification fraction on nitric oxide synthase in improving erectile dysfunction with particular focus on the nitric oxide (NO)-cGMP pathways. Two rat models, 9-month-old SD rats and 11-month-old SD rats, were given VAS(40 mg/kg per day) for 4 days, The aqueous fraction of silworm male pupae extract; semi-purified VAS(100 mg/kg per day) for 10 days, respectively. The NOS activities of the following three enzymes were examined: neuronal NO synthase(nNOS), inducible NOS(iNOS), endothelial NOS(eNOS), vascular endothelial growth factor on endothelial cells(VEGF) and anti-inflammation effect of Tumor necrosis factor-$\alpha$. The results showed increases in the nitric oxide synthase activities. Western blotting of the tissue homogenate showed an increase in the nNOS level in the brain and tongue, and an increase in the endothelial NO synthase(eNOS) level in penis. However, there was little association with VEGF production in HUVEC endothelial cells and no relationship with TNF-$\alpha$ which showed low levels.

Keywords

References

  1. Ahn, M.Y., Shim, S.H., Jeong, H.K. and Ryu, K.S. (2008). Purification of a dimethyladenosine compound from silkworm pupae as a vasorelaxation substance. J. Ethnopharm., 117, 115-122 https://doi.org/10.1016/j.jep.2008.01.031
  2. Ahn, M.Y., Heo, J.E., Ryu, J.H., Jeong, H.K. and Chung, W.T. (2007). Antioxidant Activity of cholesterol derived from silkworm pupae. Natl. Prod. Sci., 13, 220-224
  3. Fessenden, J.D. and Schacht, J. (1998). The nitric oxide/cyclic GMP pathway: A potential major regulator of cochlear physiology. Hearing Research, 118, 168-176 https://doi.org/10.1016/S0378-5955(98)00027-6
  4. Crane, M.S., Rossi, A.G. and Megson, I.L. (2005). A potential role for extracellular nitric oxide generation in cGMPindependent inhibition of human platelet aggregation: biochemical and pharmacological considerations. Br. J. Pharmacol., 144, 849-859 https://doi.org/10.1038/sj.bjp.0706110
  5. Kukreja, R.C., Ockaili, R., Salloum, F., Yin, C., Hawkins, J., Das, A. and Xi, L. (2004). Cardioprotection with phosphodiesterase- 5 inhibition a novel preconditioning preconditioning strategy. J. Mol. Cell. Cardiol., 36, 165-173 https://doi.org/10.1016/j.yjmcc.2003.11.001
  6. Geldof, A.A., Mastbergen, S.C., Henrar, R.E. and Faircloth, G.T. (1999). Cytotoxicity and neurocytotoxicity of new marine anticancer agents evaluated using in vitro assays. Cancer Chemother. Pharm., 44, 312-318 https://doi.org/10.1007/s002800050983
  7. Lee, S.J. and Lee, H.K (2005). Sanguiin H-6 blocks endothelial cell growth through inhibition of VEGF binding to WEGF receptor. Arch. Phram. Res., 11, 1270-1274 https://doi.org/10.1007/BF02978211
  8. Lee, H., Baek, S., Joe, S.J. and Pyo, S.N. (2006). Modulation of IFN-$\gamma$ production by TNF-$\alpha$ in macrophages from the tumor environment: Significance as an angiogenic switch. Internat. Immunopharm., 6, 71-78 https://doi.org/10.1016/j.intimp.2005.08.010
  9. Li, Z., Xi, X., Gu, M., Feil, R., Ye, R.D., Eigenthaler, M., Hofmann, F. and Du, X. (2003). A stimulatory role for cGMPdependent protein kinase in platelet activation. Cell, 112, 77-86 https://doi.org/10.1016/S0092-8674(02)01254-0
  10. Park, C.S., Ryu, S.D. and Hwang, S.Y. (2004). Elevation of intracavernous pressure and NO-cGMP activity by a new herbal formula in penile tissues of aged and diabetic rats. J. Ethnopharm., 94, 85-92 https://doi.org/10.1016/j.jep.2004.04.028
  11. Ryu, K.S., Ahn, M.Y., Lee, H.S., Kim, I., Kim, J.W., Kim, S.H. and Choi, J.H. (2002). The tonic effect of the extract from male silkworm (Bombyx mori L.) pupae on rats. IJIE., 5, 123-126
  12. Succu, S., Cocco, C., Stefania, M., Melis, M., Melis. M.R., Possenti, R., Levi, A., Ferri, G.L. and Argiolas, A. (2004). Pro-VGF-derived peptides induce penile erection in male rats: possible involvement of oxytocin. Eur. J. Neurosci., 20, 3055-3040
  13. Toda, N., Ayajiki, K. and Okamura, T. (2005). Nitric oxide and penile erectile function. Pharmacology & Therapeutics, 106, 233-266 https://doi.org/10.1016/j.pharmthera.2004.11.011
  14. Zhang, C., Xiangbin, X., Potter, B.J., Wang, W., Kuo, L., Michael, L., Bagby, G. and Chilian, W.M. (2006). TNF-a contributes to endothelial dysfunction in ischemia/reperfusion injury. Arteriorscler Thromb Vasc Biol., 26, 475-480
  15. Zhang, R., Wang, L., Zhang, L., Chen, J., Zhu, Z., Zhang, Z. and Chopp, M. (2003). Nitric oxide enhances angiogenesis via the synthesis of vascular endothelial growth factor and cGMP after stroke in rat. Circ. Res., 92, 308-313 https://doi.org/10.1161/01.RES.0000056757.93432.8C
  16. Ziche, M. and Morbidelli, L. (2000). Nitric oxide and angiogenesis. J. Neuro-Oncology, 50, 139-148 https://doi.org/10.1023/A:1006431309841