• Asian-Australasian Journal of Animal Sciences
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• v.14 no.2
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• pp.163-169
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• 2001

Despite the high potency of the retrovirus vector system in gene transfer, one of the main drawbacks of has been difficulty in preparing highly concentrated virus stock. Numerous efforts to boost the virus titer have ended in unsatisfactory results mainly due to fragile property of retrovirus envelope protein. In this study, to overcome this problem, we constructed our own retrovirus vector system producing vector viruses encapsulated with VSV-G (vesicular stomatitis virus G glycoprotein). Concentration process of the virus stock by ultracentrifuge did not sacrifice the virus infectivity, resulting in more than 108 to 109 CFU (colony forming unit) per ml on most of the target cell lines tested. Application of this high-titer retrovirus vector system was tested on chicken embryos. Injection of virus stock beneath the blastoderms of pre-incubated fertilized eggs resulted in chick embryos expressing E. coli LacZ gene with 100% efficiency. Therefore, our results suggest that it is possible to transfer the foreign gene into chicken embryo using our high-titer retrovirus vector.

• Korean Journal of Cognitive Science
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• v.4 no.1
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• pp.243-260
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• 1993

This paper presents an efficient method for estimationg and correcting the slant of off-line handwritten Hangul strings.In the proposed method,after extracting contours from input image.Hough tranform is applied to the contours to detect lines and estimate slants of the lines.When Hough trans form is applied to the contours,pixels which are not parts of the same stroke could be detected as a line.In order to exclude these lines from slant estimation process,detected lines which have the length less than threshold are eliminated.Experiments have been performed with address images which were extracted from live envelopes provided by Seoul Mail Center.Experimental results show that the proposed method is superior to the previous methods,which had been done with handwritten English strings.in estimation the slant of off-line handwritten Hangul strings.

• KIEAE Journal
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• v.17 no.3
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• pp.5-13
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• 2017

Purpose: Various studies have been performed to address the issue of increased energy use by buildings. In particular, research on complex envelopes that combines diverse envelope element techniques is currently in progress in the building sector. The present study aimed to develop an awning system using a light shelf, and to verify its validity through performance evaluation. Method: In the present study, a testbed was established for the performance evaluation of the awning system using a light shelf, and the uniformity ratio and lighting energy consumption were compared and analyzed relative to those with no awning and light shelf installation (Case 1), awning installation (Case 2), and light shelf installation (Case 3). Result: 1) In the present study, an awning system using a light shelf (Case 4) where an opening is made on the awning screen and natural light can be introduced through the light shelf located at the bottom was developed. 2) The optimum standard for Case 4 obtained through the performance evaluation was a 0.6m lighting length and a 2m extension length at a light shelf angle of $30^{\circ}$. 3) Case 4 with the optimum standard had a 5.5% lower uniformity ratio than Case 2, but had a higher uniformity ratio than Case 1 and Case 3. 4) Case 4 with the optimum standard showed 13.3%, 44.6%, and 0%~8.7% lighting energy reductions compared to Case 1, Case 2, and Case 3, respectively. 5) Based on the above results, Case 4 suggested in the present study was found to be effective for indoor light environment improvement and lighting energy reduction.

• Korean Institute of Interior Design Journal
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• v.22 no.1
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• pp.11-18
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• 2013

The Contemporary consumer society demands companies to emphasize more on the brand marketing that can deliver the brand identities distinguished from those of competing companies. In today's environment, the meaning of consumption is not only a simple purchase of a product, but also assimilation into images, experience and emotional aspects of the brand. The flagship stores of brands take the advantage of the tendency seriously and utilize the envelopes of the building as marketing catalysts to establish their brand identities. This paper aims to investigate the possibility of the architectural envelope as a marketing element to establish the brand identities, by analyzing the design techniques that enhance the visual effects utilizing innovative application of material, pattern and connection details. it is studied that the function of architectural envelope's change from past to recent date and role of architectural envelope as means of marketing for expression brand image in contemporary commerce space and elements that can be applied on architectural envelope with theoretical consideration about marketing and brand identity. Also this paper will research about experience elements because the process for consumers to recognize brand image with architecture is the process of visual experience. And through example research, this paper will study interrelationship between visual system of cognition to be expressed on architectural envelope.

• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.470-478
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• 2012

Recent genome comparisons of E. coli B and K-12 strains have indicated that the makeup of the cell envelopes in these two strains is quite different. Therefore, we analyzed and compared the envelope proteomes of E. coli BL21(DE3) and MG1655. A total of 165 protein spots, including 62 nonredundant proteins, were unambiguously identified by two-dimensional gel electrophoresis and mass spectrometry. Of these, 43 proteins were conserved between the two strains, whereas 4 and 16 strain-specific proteins were identified only in E. coli BL21(DE3) and MG1655, respectively. Additionally, 24 proteins showed more than 2-fold differences in intensities between the B and K-12 strains. The reference envelope proteome maps showed that E. coli envelope mainly contained channel proteins and lipoproteins. Interesting proteomic observations between the two strains were as follows: (i) B produced more OmpF porin with a larger pore size than K-12, indicating an increase in the membrane permeability; (ii) B produced higher amounts of lipoproteins, which facilitates the assembly of outer membrane ${\beta}$-barrel proteins; and (iii) motility- (FliC) and chemotaxis-related proteins (CheA and CheW) were detected only in K-12, which showed that E. coli B is restricted with regard to migration under unfavorable conditions. These differences may influence the permeability and integrity of the cell envelope, showing that E. coli B may be more susceptible than K-12 to certain stress conditions. Thus, these findings suggest that E. coli K-12 and its derivatives will be more favorable strains in certain biotechnological applications, such as cell surface display or membrane engineering studies.

• Molecules and Cells
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• v.39 no.9
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• pp.669-679
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• 2016

N-acetyl-D-glucosamine kinase (GlcNAc kinase or NAGK) primarily catalyzes phosphoryl transfer to GlcNAc during amino sugar metabolism. Recently, it was shown NAGK interacts with dynein light chain roadblock type 1 (DYNLRB1) and upregulates axo-dendritic growth, which is an enzyme activity-independent, non-canonical structural role. The authors examined the distributions of NAGK and NAGK-dynein complexes during the cell cycle in HEK293T cells. NAGK was expressed throughout different stages of cell division and immunocytochemistry (ICC) showed NAGK was localized at nuclear envelope, spindle microtubules (MTs), and kinetochores (KTs). A proximity ligation assay (PLA) for NAGK and DYNLRB1 revealed NAGK-dynein complex on nuclear envelopes in prophase cells and on chromosomes in metaphase cells. NAGK-DYNLRB1 PLA followed by Lis1/NudE1 immunostaining showed NAGK-dynein complexes were colocalized with Lis1 and NudE1 signals, and PLA for NAGK-Lis1 showed similar signal patterns, suggesting a functional link between NAGK and dynein-Lis1 complex. Subsequently, NAGK-dynein complexes were found in KTs and on nuclear membranes where KTs were marked with CENP-B ICC and nuclear membrane with lamin ICC. Furthermore, knockdown of NAGK by small hairpin (sh) RNA was found to delay cell division. These results indicate that the NAGK-dynein interaction with the involvements of Lis1 and NudE1 plays an important role in prophase nuclear envelope breakdown (NEB) and metaphase MT-KT attachment during eukaryotic cell division.

• Korean Journal of Animal Reproduction
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• v.25 no.2
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• pp.171-180
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• 2001

Compared to other gene transfer system, the advantages of retrovirus-mediated gene transfer are technical ease, efficient expression and genetic stability. Despite the high potency of the retrovirus vector system in gene transfer, one of the drawbacks is a difficulty in concentration of virus stock. To overcome this problem, we tested a new retrovirus vector system producing the progeny retrovirus particles encapsidated with VSV-G (vesicular stomatitis virus G glycoprotein). The infectivity of this virus was not sacrificed by ultracentrifugal concentration and the host cell range extended from all mammalian to fish embryos. Virus titer after 1,000 x concentration was more than 10$^{8}$ CFU/ $m\ell$ on most of the target cell lines. We applied this pantropic viruses in transgenic chicken production by injecting the concentrated (100$\times$) stock into subgerminal cavity of stage X chicken embryos. The survival rate of chicken embryos after injection was about 20% and gene integration rate in surviving embryos was scored almost 100%. Analyses of RT-PCR and fluorescence microscopy, however, showed no evidence of the transgene expression.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.43 no.3
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• pp.204-212
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• 2015

The infrared lock-on range of target aircraft plays a critical role in determining the aircraft survivability. In this investigation, the effects of various UAV engine nozzle configurations on the aircraft lock-on range were theoretically analyzed. A virtual subsonic aircraft was proposed first, based on the mission requirement and the engine performance analysis, and convergent-type nozzles were then designed. After determining thermal flow field and nozzle surface temperature distribution with the CFD code, an additional analysis was conducted to predict the IR signature. Also, atmospheric transmissivity for various latitude and seasons was calculated, using the LOWTRAN code. Finally, the lock-on and lethal envelopes were calculated for different nozzle configurations, assuming the sensor threshold of the given IR guided missile. It was shown that the maximum 55.3% reduction in lock-on range is possible for deformed nozzles with the high aspect ratio.

• Korean Chemical Engineering Research
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• v.48 no.6
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• pp.763-767
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• 2010

High-pressure phase behavior for the binary mixture of 1-propanol with supercritical $CO_2$ has been measured by means of a high-pressure phase equilibrium apparatus equipped with a variable-volume view cell. The equilibrium loci of the pressure - composition and pressure - temperature were obtained for the binary mixture of 1-propanol + $CO_2$ system at 305.15 K, 313.15 K, 323.15 K and 333.15 K, and from 2 MPa to 11 MPa. The critical temperature of the mixture increased with the temperature. The pressure-composition line for the binary mixture of $CO_2$-1-propanol system showed a typical type-II phase behavior. The experimental P-x envelopes were correlated by using the Peng-Robinson equation of state in a satisfactory manner to obtain the parameters with $k_{ij}=0.116$ and ${\eta}_{ij}=-0.065$.

• Structural Engineering and Mechanics
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• v.41 no.4
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• pp.495-508
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• 2012

The strength theory of concrete is significant to structure design and nonlinear finite element analysis of concrete structures because concrete utilized in engineering is usually subject to the action of multi-axial stress. Experimental results have revealed that lightweight aggregate (LWA) concrete exhibits plastic flow plateau under high compressive stress and most of the lightweight aggregates are crushed at this stage. For the purpose of safety, therefore, in the practical application the strength of LWA concrete at the plastic flow plateau stage should be regarded as the ultimate strength under multi-axial compressive stress state. With consideration of the strength criterion, the ultimate strength surface of LWA concrete under multi-axial stress intersects with the hydrostatic stress axis at two different points, which is completely different from that of the normal weight concrete as that the ultimate strength surface is open-ended. As a result, the strength criteria aimed at normal weight concrete do not fit LWA concrete. In the present paper, a multi-axial strength criterion for LWA concrete is proposed based on the Unified Twin-Shear Strength (UTSS) theory developed by Prof Yu (Yu et al. 1992), which takes into account the above strength characteristics of LWA under high compressive stress level. In this strength criterion model, the tensile and compressive meridians as well as the ultimate strength envelopes in deviatoric plane under different hydrostatic stress are established just in terms of a few characteristic stress states, i.e., the uniaxial tensile strength $f_t$, the uniaxial compressive strength $f_c$, and the equibiaxial compressive $f_{bc}$. The developed model was confirmed to agree well with experimental data under different stress ratios of LWA concrete.