• 한국해양환경ㆍ에너지학회지
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• 제2권1호
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• pp.102-112
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• 1999

히로시마만에 존재하고있는 간석지에 있어서 유입하천의 유무, 폐쇄성 등의 환경조건이나 조성 후 경과연수가 다른 인공간석지 7개소와 자연간석지 3개소를 선정해 간석지 토양에 있어서 물리화학적 및 생물학적인 구조나 유기물분해기능에 관해 비교 검토하였다. 또한 인공간석지에 있어서는 입지장소, 조성방법, 조성 후 경과연수 등의 차이에 의한 영향, 자연간석지와 동일하게 조성하기 위한 조성인자를 검토해서, 아래와 같은 결과를 얻었다. 인공간석지는 자연간석지와 다른 특성을 갖고 있는 것과 그렇지 못한 것으로 분리되었다. 그렇지만 대부분이 자연간석지와 전혀 다른 특성을 나타내었다 토양구조 중에서 가장 다르게 나타난 것은 세균수, 실트함유량, 유기물함유량을 들 수 있으며, 각 실험 항목이 인공간석지 보다 자연간석지가 높은 값을 나타내었다. 자연간석지와 현저하게 차이를 보인 세균수, 실트함유량 및 유기물함유량에 관해서, 각 인공간석지의 조성 후 경과연수, 표면구배, 유입 하천의 유무, 폐쇄성 등의 조성 인자와의 상관성을 분석 했지만, 명확한 상관관계는 알 수 없었다. 자연간석지와 유사한 토양구조 특성을 갖기 위한 간석지를 조성하는 데에는, 간석지 전면에 있어서 해수로부터 실트 성분이 침강 가능한 장소의 선정이 중요하다.

• 한국가축번식학회지
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• 제17권2호
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• pp.85-92
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• 1993

In the previous study, we observed that Purine has a time dependent effect in maintaining the oocytes in meiotic arrest, and human fetal cord serum(HFCS) and human mature follicular fluid(HMFF) reverse the GVBD suppressed by purines. And it was reported that purine has a harmful effect on the development of oocytes or embryos, when they were cultured for a long time, in vitro. Therefore this study was performed to investigate the effects of purine on extrusion rates of 1st pb and viability of oocytes cultured for a long time, in vitro. Immature oocytes(GV stage) were collected from ovaries of 25~28 day old ICR mice at 48 hrs after PMSG injection. Cumulus-enclosed and denuded oocytes collected were assigned randomly to one of several culture conditions. Some of the oocytes were cultured in 4mM hypoxanthine for 24hr, and the extrusion rates of 1st pb and viability of the oocytes were assessed at every 12 hrs. In the viability, the oocytes showed granulation, pigmentation of cytoplasm or lysis of 1st pb extruded were regarded as degenerating oocytes. Also some of the oocytes were cultured in hypoxanthine for 12 hrs then the resulting oocytes were transferred to hypoxanthine-free medium and cultured for 12 hrs to determine whether the inhibitory effect of hypoxanthine on the 1st pb extrusion was reversible. The rest of the oocytes were cultured in medium containing hypoxanthine and adenosine for 18 hrs to compare the 1st pb extrusion be attendant upon hte concentration of HFCS or HMFF supplemented. Hypoxanthine suppressed the extrusion of 1st pb and viability of the oocytes significantly, when they were cultured for more than 12 hrs and the harmful effect of hypoxanthine was showed in denuded oocytes, prominently. The suppressive effect of hypoxanthine was reversed by just removal of the hypoxanthine from the cultrue medium. Also there was no difference in reverse the pb extrusion rate suppressed between HFCS and HMFF. The extrusion rate of 1st pb in medium containing adenosine and hypoxanthine was increased in line with the concentration of HFCS or HMFF supplemented. Hypoxanthine suppressed the extrusion of 1st pb and viability of the oocytes significantly, when they were cultured for more than 12 hrs and the harmful effect of hypoxanthine was showed in denuded oocytes, prominetly. The suppressive effect of hypoxanthine was reversed by just removal of the hypoxanthine fromthe culture medium. Also there was no difference in reverse the pb extrusion rate suppressed between HFCS and HMFF. The extrusion rate of 1st pb in medium containing adenosine and hypoxanthine was increased in line with the concentration of HFCS or HMFF supplemented.

• 한국가축번식학회지
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• 제17권2호
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• pp.141-148
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• 1993

This studies were carried out to investigate the effects of co-culture with cumulus cells, oviduct epithelial cells and uterine endometrium cells on the in-vitro fertilization and cleavage rate of bovine follicular oocytes. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes surrounded with cumulus cells were recovered by aspirating follicular fluids from the visible follicles of diameter 3~5 mm. The follicular oocytes were cultured in TCM-199 medium containing hormones and 10% FCS for 24~48 hrs in a incubator with 5% CO2 in air at 38.5$^{\circ}C$ and then matured oocytes were again cultured for 12~18 hrs with motile capacitated sperm by preincubation. The results obtained in these experiments were summarized as follows: 1. The in vitro maturatin and fertilization rate of bovine oocytes co-cultured with cumulus cells in TCM-199 medium were 64.0~74.1% and 40.0~58.6% respectively. And in-vitro fertilization rate of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in-vitro maturatin and fertilization rate of bovine oocytes co-cultured with 1$\times$104 cells/ml, 1$\times$106 cells/ml, 1$\times$108 cells/ml and 1$\times$1015 cells/ml oviduct epithelial cells in TCM-199 medium were 59.3% and 40.7%, 64.0% and 48.0%, 58.3% and 37.5%, 52.0% and 32.0%, respectively. 3. The in-vitro maturation and fertilization rate of bovine oocytes co-cultured with 1$\times$104 cells/ml, 1$\times$106 cells/ml, 1$\times$108 cells/ml and 1$\times$1015 cells/ml uterine endometrium cells in TCM-199 medium were 56.0% and 36.0%, 60.7% and 42.9%, 59.3% and 37.0%, 52.0% and 36.0%, respectively. 4. When the in-vitro fertilized oocytes were co-cultured with cumulus cells, oviduct epithelial cells and uterine endometrium cells, the development rate to be blastocyst was 12.2%, 15.6% and 11.7%, respectively and rates were higher than that of control, 2.1%(P<0.05).

• Asian-Australasian Journal of Animal Sciences
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• 제22권3호
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• pp.328-335
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• 2009

This study was undertaken to investigate the influence of exposure and removal of four different cryoprotectants (CPAs) on the ATP content of cumulus cell-enclosed (COs) and cumulus cell-denuded (DOs) immature porcine oocytes. The in vitro nuclear maturation of the COs, exposed to and removed from the CPAs was also assessed. Both COs and DOs were exposed to 1.5 M concentrations of each CPA (ethylene glycol (EG); propylene glycol (PG); dimethyl-sulfoxide (DMSO); and glycerol (G)) for durations of 5, 15, and 30 minutes at room temperature ($23.5{\pm}1.5^{\circ}C$), and immersed in physiological saline supplemented with 20% (v/v) fetal bovine serum for 5 minutes ($39^{\circ}C$) to remove each CPA. Before, during and after exposure to each CPA, the ATP content of both the COs and the DOs was measured. After removal from each CPA an aliquot of the COs was matured for 44${\pm}$2 h, and their nuclear maturation rates were measured up to the metaphase stage of the second meiotic division (the M-II stage). The maturation rates up to the M-II stage were not significantly different between all the groups that were exposed to each CPA for 5 minutes. For 15 and 30 minute exposures, the maturation rates of the COs exposed to PG, DMSO and EG were almost the same as those of the control groups; however, the rates of G group exposed for 15 and 30 minutes were significantly lower (p<0.05) than the control group. These groups were also found to have a decrease in the ATP content of COs and DOs during and after exposure for the same periods (p<0.05). In addition, although the ATP contents of the COs after exposure to EG for any period were the same as the controls, the ATP content of the DOs exposed to EG for any period were significantly lower (p<0.05) than those of the controls. When the ATP content of the COs and DOs of each CPA were compared, the DOs exposed to PG were found to have a significantly greater level (p<0.05) than DOs exposed to G for any duration. In addition, the ATP content of DOs exposed to PG for 30 min and removal was also higher (p<0.05) than when exposed to DMSO for the same period. These findings indicate that PG may be a useful CPA for the cryopreservation of immature porcine oocytes.

• 한국방재학회 논문집
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• 제10권4호
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• pp.63-68
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• 2010

지하공동구는 도시 및 공동주택 단지에 반드시 필요한 전력, 통신, 가스, 송유관, 급 배수, 에너지시설 등을 효과적으로 수용하고 도시기반시설의 확장에 따라 파생되는 여러 가지의 문제점을 해결하기 위한 방안으로 시설물을 한곳으로 모아 유지 및 관리하는 시설이다. 지하공동구가 훼손될 경우 국가안보는 물론 사회전반에 미치는 파장이 지대하므로 화재예방 활동 및 진압대 책상 취약점 등을 분석하여 우리 실정에 맞는 방재 대책을 도출하여 공동구의 설계, 제도적인 문제, 방화시설의 문제, 화재확산현상 등을 분석하여 종합적인 화재위험을 분석하여 화재예방활동과 진압대책을 수립하여야 한다. 지하공동구는 밀폐 폐쇄된 공간이므로 산소공급 부족으로 인한 불완전 연소 등으로 농연, 일산화탄소 등 유독성연소생성물의 발생과 열기의 충만 및 시야장해로 진입이 곤란 하는 문제점이 있다. 이러한 현상은 지상화재보다는 지하 화재의 특수성에 비추어 볼 때 화재예방활동은 물론이고 소방전술에서 취약하므로 화재의 효율적인 소방전술이 되기 위해서는 화재의 특이성을 감안하여 유관기관에서는 현장대응 지휘체제를 확립하고, 대응기관에서는 평상시 공동구에 대한 구조파악과 필요한 각종 정보를 수집 기록하고 24시간 관리공조체제 유지 및 대응기관에서 가상종합훈련을 강화하여야하며 근본적으로 공동구내에 설치되어 있는 케이블의 난연화 불연화 및 연소 확대 방지를 위한 대책 등이 필요하다.

• 한국산학기술학회논문지
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• 제17권11호
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• pp.629-634
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• 2016

본 연구에서는 철도차량 내장재 화재성능평가기준 중 연기밀도 측정에서 주요 인자들의 영향을 합성고무바닥재를 이용하여 분석 및 검토하였다. 밀폐된 공간에서 운영되는 철도차량의 특성 상, 객실 내부에서 화재 발생 시에 연기로 인해 막대한 인명피해가 발생할 수 있다. 따라서 국내 외의 철도운영국가에서는 내장재의 연기 발생량을 엄격하게 규제하고 있으며, 이를 위해 내장재의 화재성능평가에 있어 연기밀도의 정확한 측정이 요구된다. 연기밀도의 측정에 있어 주요 영향인자로 시료 고유의 균질도, 장비를 이용한 조건 및 측정의 편차 그리고 장비를 운용 관리하는 환경의 영향을 꼽을 수 있다. 각 인자별 연기밀도에 영향도를 확인하기 위하여 불확도 추정에 따른 기여율 분석을 수행하였다. 그 결과, 광센서(PM tube)를 이용한 투과율 측정편차의 기여율이 약 86 %로 연기밀도 측정의 대표 영향인자임을 확인할 수 있었다. 그리고 높은 균질도를 가지는 시료를 대상으로 한 연기밀도 측정에서는 그 외 영향인자(시료연소용 복사열 조건(${\pm}0.5 kW/m^2$) 및 장비의 운용 관리환경)의 기여율이 약 11 %로 낮음을 확인할 수 있었다.

• 한국공간구조학회논문집
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• 제8권4호
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• pp.47-55
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• 2008

이 논문은 지오데식 돔 구조물의 구조최적화에 대한 연구내용과 관련이론 그리고 수치해석결과를 기술하고 있다. 지오데식 돔의 공간 효율성을 알아보기 위해 돔의 기저인 정이십면체의 외피면적과 내부공간의 비율을 계산하고 건축구조물에서 나타나는 다른 입방체의 값과 정량적으로 비교하였다. 지오데식 돔을 형성하기 위한 절차를 세부적으로 정리하고 이를 프로그래밍하여 설계최적화프로그램 ISADO-OPT에 연동하였다. 본 연구에서는 반구 형태의 지오데식 돔이 집중하중에 효율적으로 저항할 수 있는 최적의 부재크기 패턴을 조사하기 위하여 수학적 프로그래밍 기법을 도입하였다. 이때 최소화해야하는 돔 전체 부재의 중량을 목적함수로 이용하고 하중이 가해지는 절점에서 발생하는 변위 값과 각 부재에서 발생하는 응력 값을 허용치 이하로 제한하는 제약조건으로 사용하였다. 목적함수와 주어진 제약조건을 만족하는 최적 설계변수값을 검색하기 위해 설계변수에 대한 목적함수의 민감도 값을 유한차분법을 이용하여 계산하였다. 본 연구에서 기술한 지오데식돔을 위한 설계최적화 기본이론을 바탕으로 도출한 최적 부재패턴은 향후 돔의 최적설계에 기본 벤치마크테스트결과로 유용하게 사용될 것으로 판단된다.

• 한국조경학회지
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• 제27권3호
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• pp.1-17
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• 1999

The purpose of this study is to evaluate the creation techniques of artificial wetland, one of biotopes developed to promote biodiversity in urban areas, and to look for improvement steps. Specifically, artificial wetland creation techniques were categorized into living environment and living creature classification. Being living conditions for creations, habitat environment was reviewed with a focus on water and soil environments. Living creatures were classified into plants, insects, fish, and birds. The evaluation of creation techniques was done in post-construction evaluation while considering the creation of habitats for living creatures. Intervention by users, changes in living environment and living species, and relevance of creation techniques were reviewed. Key results of this study are as follows. (1) Water environment for the living environment of creatures provides a suitable environment conditions for the living of creatures through a process easing the use of piped water. Various water depths and embankment appear to have a positive impact on the living of aquatic life. In particular, embankment covered in soil naturally played an important role as a place for the activities of aquatic insects and young fish as well as the growth of aquatic plants. (2) Various aquatic and ground plants to promote insect-diversity, shallow water, and old-tree logs had contributed greatly in increasing the types and number of insects. Aquatic insects. Aquatic insects were seen much particularly in areas where aquatic plants are rich but water is shallow than any other areas. (3) A space piled with stone to provide habitats for fish was not much used. However, it was observed that fish used embankment built with natural stones and embankment using logs in areas where water is deep. In addition, it was confirmed that 1,500 fish that had been released propagated using various depths and places for birth. (4) It was analyzed that techniques (creation of island, log setting, and creation of man-made bird nests) to provide habitats and to attract birds are not serving their roles. In such a case, it is believed that species had not increased due to the smallness as well as isolated features of the area. Based on theoretical review, they are judged to be areas that are likely to be used when a greater variety of birds is introduced. It is judged that attracting and keeping more birds at the site, such spaces need to be linked systematically in the future in terms of building eco-network while ensuring an adequate living areas. (5) In the study areas, users intervened greatly. As a result, a blockage was created preventing the normal growth of plants and non-indigenous plants were introduced. In order to limit the intervention by users, setting enough buffer zones, and environment education programs were urgently required. D/H=1>Hyangkyo> houses on the river>temples>lecture halls. D/H ratio of the backside areas is as follows. D/H=1>Hyangkyo>houses on the river>lecture halls. 4. Inner garden were planted deciduous than evergreen trees with Lagerstroemia indica. Enclosed dominant trees were planted by Pinus densiflora, Querces seuata. construct GEM strain, and examined for the expression and functional stability in microcosms.

• 한국조경학회지
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• 제28권5호
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• pp.68-75
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• 2000

The purpose of this paper is to define the spatial scope and landscape structure of the capital city of Shilla Dynasty. We have tried to analyze the locational characteristics, the location and landscape form of the Yonggang-Dong pond site which was assumed a part of a king's place, and had the important implicitness in delineating the spatial scope of capital city of Shilla Dynasty. The research took the theory of landscape cognition as a tool, and processed with the book review related simultaneously. The results of study are as follows; 1) It has been almost conformed that the Yonggang-Dong Pond Site was a part of the North Palace of Shilla Dynasty, and it took the form of fully enclosed by the forest, such as the names of Imjungsu and Gosungsu, and so on. This fact has leaded to the special landscape form of 'Forest with Palace' that is an similar pattern of Forest with Tumulus, Forest having Well, Forest having Well, Forest having Mountain. Each of them is symbolizing the place of holiness in the Shilla Dynasty. 2) The Yonggang-Dong Pond site and its surrounding forests made them together be the north border of the Capital City of Shilla Dynasty. This fact made it possible to consider the form of landscape of the Najung Forest with Posugjung Pavilion as the same on which was on the southern border of the city. 3) The above mentioned facts lead to clues that the border of the capital city of Shilla Dynasty does mean that it was not the mere functional one, but symbolic one. Thus, this paper suggests that the spatial scope of the capital city of Shilla Dynasty should include not only the area of block system, but also the surrounding natural area of mountains, forests, and so on, including the artificial elements of palace and pavilion. 4) This type of borders with the natural elements show basically the different type and function. We could find out some examples from China's and Japan under the same block systems and in the same era. It should be more identifiable in the form and the meaning of landscape of the capital city of Silla Dynasty in ancient Korea.

• 정보처리학회논문지B
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• 제11B권3호
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• pp.309-316
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• 2004

본 논문은 움직임 벡터를 보다 빠르고 정확하게 추정해나가는 탐색 방법으로 상 좌 우 3 방향을 고려한 TDS(T-Shape Diamond Search) 알고리즘을 제안하였다. 이 방법에서는 실제 움직임 벡터가 탐색 영역의 중심과 상ㆍ하 ㆍ좌 ㆍ우 방향에 집중되어 있는 특성을 이용하여 먼저 탐색 원점을 중심으로 상ㆍ하ㆍ좌ㆍ우 4 방향으로 탐색 점을 배치한 후 블록 정합을 실행한다 이들 중 정합 오차가 가장 삭은 지점을 기준점으로 상 방향으로 탐색 점을 확장하여 정합 오차를 측정해보고 기준점토다 오차가 작으면 계속 상 방향으로 확장해 나가고 그렇지 않으면 기준점을 충심으로 좌우 두 점 중 정합오차가 작은 점을 선택한다. 예측된 방향으로 위의 과정을 반복하며 움직임을 추정한다. 특히 움직임이 십자방향에 집중되는 영상의 경우 접근이 빠르고, 단계적으로 움직임 가능성이 낮은 부분을 탐색 대상에서 제외해 나감으로써 탐색이 비교적 빠르고 정확하게 이루어진다. 이 방법은 기존의 부분 최적 탐색 기법인 NTSS, DS, 그리고 HEXBS 등의 탐색법과 비교할 때 유사한 화질을 유지하면서도 탐색 점수에서는 평균 38％의 개선된 결과를 얻을 수 있었다. 특히 움직임이 적은 영상에서의 탐색 점수는 50％의 향상된 결과를 얻었다.