• Tissue Engineering and Regenerative Medicine
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• 제15권6호
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• pp.711-719
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• 2018

BACKGROUND: Collagen organization within tissues has a critical role in wound regeneration. Collagen fibril diameter, arrangements and maturity between connective tissue growth factor (CTGF) small interfering RNA (siRNA) and mismatch scrambled siRNA-treated wound were compared to evaluate the efficacy of CTGF siRNA as a future implement for scar preventive medicine. METHODS: Nanocomplexes of CTGF small interfering RNA (CTGF siRNA) with cell penetrating peptides (KALA and $MPG^{{\Delta}NLS}$) were formulated and their effects on CTGF downregulation, collagen fibril diameter and arrangement were investigated. Various ratios of CTGF siRNA and peptide complexes were prepared and down-regulation were evaluated by immunoblot analysis. Control and CTGF siRNA modified cells-populated collagen lattices were prepared and rates of contraction measured. Collagen organization in rabbit ear 8 mm biopsy punch wound at 1 day to 8 wks post injury time were investigated by transmission electron microscopy and histology was investigated with Olympus System and TS-Auto software. CONCLUSION: CTGF expression was down-regulated to 40% of control by CTGF siRNA/KALA (1:24) complexes (p<0.01) and collagen lattice contraction was inhibited. However, down-regulated of CTGF by CTGF $siRNA/MPG^{{\Delta}NLS}$ complexes was not statistically significant. CTGF KALA-treated wound appeared with well formed-basket weave pattern of collagen fibrils with mean diameter of $128{\pm}22nm$ (n = 821). Mismatch siRNA/KALA-treated wound showed a high frequency of parallel small diameter fibrils (mean $90{\pm}20nm$, n = 563). CONCLUSION: Controlling over-expression of CTGF by peptide-mediated siRNA delivery could improve the collagen orientation and tissue remodeling in full thickness rabbit ear wound.

• Korean Journal of Radiology
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• 제23권7호
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• pp.763-772
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• 2022

Objective: Preoperative differential diagnosis of follicular-patterned lesions is challenging. This multicenter cohort study investigated the clinicoradiological characteristics relevant to the differential diagnosis of such lesions. Materials and Methods: From June to September 2015, 4787 thyroid nodules (≥ 1.0 cm) with a final diagnosis of benign follicular nodule (BN, n = 4461), follicular adenoma (FA, n = 136), follicular carcinoma (FC, n = 62), or follicular variant of papillary thyroid carcinoma (FVPTC, n = 128) collected from 26 institutions were analyzed. The clinicoradiological characteristics of the lesions were compared among the different histological types using multivariable logistic regression analyses. The relative importance of the characteristics that distinguished histological types was determined using a random forest algorithm. Results: Compared to BN (as the control group), the distinguishing features of follicular-patterned neoplasms (FA, FC, and FVPTC) were patient's age (odds ratio [OR], 0.969 per 1-year increase), lesion diameter (OR, 1.054 per 1-mm increase), presence of solid composition (OR, 2.255), presence of hypoechogenicity (OR, 2.181), and presence of halo (OR, 1.761) (all p < 0.05). Compared to FA (as the control), FC differed with respect to lesion diameter (OR, 1.040 per 1-mm increase) and rim calcifications (OR, 17.054), while FVPTC differed with respect to patient age (OR, 0.966 per 1-year increase), lesion diameter (OR, 0.975 per 1-mm increase), macrocalcifications (OR, 3.647), and non-smooth margins (OR, 2.538) (all p < 0.05). The five important features for the differential diagnosis of follicular-patterned neoplasms (FA, FC, and FVPTC) from BN are maximal lesion diameter, composition, echogenicity, orientation, and patient's age. The most important features distinguishing FC and FVPTC from FA are rim calcifications and macrocalcifications, respectively. Conclusion: Although follicular-patterned lesions have overlapping clinical and radiological features, the distinguishing features identified in our large clinical cohort may provide valuable information for preoperative distinction between them and decision-making regarding their management.

• Journal of Forest and Environmental Science
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• 제33권1호
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• pp.74-77
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• 2017

The purpose of this study is to analyze the effect of thinning intensity on the growth of large diameter trees in Korean white pine (Pinus koraiensis S. et Z.) plantation. Eight thinning plots were analyzed by categorizing into heavy thinning, light thinning, no thinning (control) according to thinning intensity. As a result, average DBH increased more in heavy thinning plots than in light thinning or unthinned plots. The number of large trees (DBH>25 cm) were obviously shown the most in heavy thinning plots. It is considered that heavy thinning is needed for the production of the large diameter trees.

• 한국정밀공학회지
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• 제22권4호
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• pp.52-59
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• 2005

When a micro hole is machined by EDM with a cylindrical electrode, the hole diameter is different at the inlet and the outlet of the micro hole. The taper shape of the micro hole is caused by not only wear of the electrode but the eroded particles. The eroded particles cause secondary discharge during machining the micro hole. As a result, the diameter of the inlet becomes larger than that of the outlet. In this paper, a new method is proposed to reduce the difference in diameter between the inlet and the outlet of the hole. Observed was that the feed depth and machining time affect the formation of taper shape On this experimental basis, ultrasonic vibration was applied to reduce machining time, and capacitance was changed during machining to use the difference in discharging energy of different capacitances. Using the proposed method, a straight micro-hole was fabricated.

• 한국분말재료학회지
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• 제18권6호
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• pp.546-551
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• 2011

Ag spot-coated Cu nanopowders were synthesized by a hydrothermal-attachment method (HA) using oleic acid capped Ag hydrosol. Cu nano powders were synthesized by pulsed wire exploding method using 0.4 mm in diameter of Cu wire (purity 99.9%). Synthesized Cu nano powders are seen with comparatively spherical shape having range in 50 nm to 150 nm in diameter. The oleic acid capped Ag hydrosol was synthesized by the precipitation-redispersion method. Oleic acid capped Ag nano particles showed the narrow size distribution and their particle size were less than 20 nm in diameter. In the case of nano Ag-spot coated Cu powders, nanosized Ag particles were adhered in the copper surface by HAA method. The components of C, O and Ag were distributed on the surface of copper powder.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2000년도 가을 학술발표회 논문집
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• pp.285-292
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• 2000

Though there has been increasing use of large diameter drilled shaft as a foundation structure of bridges, current practice for quality control is to confirm the minimum required load carrying capacity during construction stage. For economic and appropriate design of drilled shaft, it is necessary to evaluate the load transfer mechanism by pile load tests during initial stage of construction and to use the test results as a feedback to a revision of initial design. In this paper, results of load tests peformed at several domestic sites are presented to investigate the load transfer characteristics of large diameter drilled shaft. It was found that most of the load on piles is sustained by shaft friction and that only small portion of the load reaches the bottom of the drilled shaft. Some test results of drilled shaft by Pile Driving Analyzer performed at same sites are also presented to compare the load transfer characteristics interpreted from static pile load tests.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 추계학술대회
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• pp.980-985
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• 2003

The present study addresses a computational work to investigate the influence of a turbulent wake flow on the pressure recovery of a subsonic diffuser. The turbulent wake is generated by a cylinder with a small diameter, which is installed at the inlet of a 2-dimensional diffuser. Computation are applied to three-dimensional steady Navier-Stokes equations. The fully implicit finite volume scheme is used to discretize the governing equations. The computational results are qualitatively well compared to the experimental results. The results show that the pressure recovery of the subsonic diffuser is dependent on the diameter and location of cylinder. It is found that a certain diameter and location of the cylinder to generate the turbulent wake give a better pressure recovery, compared with no cylinder flow.

• 한국정밀공학회지
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• 제18권5호
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• pp.50-56
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• 2001

Fabrication methods are shown to produce slender and cylindrical tungsten shafts by electrochemical etching. The shape of microshatf formed by electrochemical etching is determined by the combination of two conflicting factors, i.e., initial shape and diffusion layer. We can obtain a desirable shaft profile by adjusting the thickness gradient of diffusion layer. The diameter of microshaft is controlled by mathematical model based on relation between process parameters and diameter.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 1996년도 제6회 학술강연회논문집
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• pp.209-216
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• 1996

The shape variation of billet was investigated by numerical method and spray forming work with variation of average substrate withdrawal velocity$\bar{V}$, withdrawal velocity change interval $\Delta$t and velocity deviation from average velocity $V_{dev}$. The shape and diameter with large$\bar{V}$, $\Delta$t, $V_{dev}$ vary seriously. When $\bar{V}$, $\Delta$t, $V_{dev}$ are smaller, the shape of billet is more simillar to that of the billet with constant withdrawal velocity. The average diameter of billet is determined by only $\bar{V}$, independent of $\Delta$t, $V_{dev}$. With $\bar{V}$, : 0.2 mm/sec $\Delta$t: 200 sec and $V_{dev}$. : 0.2mm/sec billet of constant diameter 230mm$\times$ height 1000mm were manufactured.

• 설비공학논문집
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• 제16권8호
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• pp.748-755
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• 2004

Flow boiling heat transfer in small-diameter round tubes has been experimentally studied. The experimental apparatus consisted mainly of refrigerant pump, condenser, receiver, test section of a 1.67 mm inner-diameter round tube and pre-heater for control of refrigerant quality at the inlet of test section. To investigate the effect of bubble nucleation site characteristics of different tube materials, three different tubes of copper, aluminum and brass were used. The ranges of the major experimental parameters were 5∼30 ㎾/$m^2$ of the wall heat flux, 0.0∼0.9 of the inlet vapor quality and the refrigerant mass flux was fixed at 600 kg/$m^2$s. The experimental results showed that the flow boiling heat transfer coefficients in small tubes were affected only by heat flux, but independent of mass flux and vapor quality. The effect of tube material on flow boiling heat transfer was observed small.