• Journal of Ginseng Research
• /
• 제44권2호
• /
• pp.300-307
• /
• 2020

Background: Emerging evidence suggests that endothelial-to-mesenchymal transition (EndMT) in endothelial dysfunction due to persistent inflammation is a key component and emerging concept in the pathogenesis of vascular diseases. Ginsenoside Rg3 (Rg3), an active compound from red ginseng, has been known to be important for vascular homeostasis. However, the effect of Rg3 on inflammation-induced EndMT has never been reported. Here, we hypothesize that Rg3 might reverse the inflammation-induced EndMT and serve as a novel therapeutic strategy for vascular diseases. Methods: EndMT was examined under an inflammatory condition mediated by the NOD1 agonist, γ-d-glutamyl-meso-diaminopimelic acid (iE-DAP), treatment in human umbilical vein endothelial cells. The expression of EndMT markers was determined by Western blot analysis, real-time polymerase chain reaction, and immunocytochemistry. The underlying mechanisms of Rg3-mediated EndMT regulation were investigated by modulating the microRNA expression. Results: The NOD1 agonist, iE-DAP, led to a fibroblast-like morphology change with a decrease in the expression of endothelial markers and an increase in the expression of the mesenchymal marker, namely EndMT. On the other hand, Rg3 markedly attenuated the iE-DAP-induced EndMT and preserved the endothelial phenotype. Mechanically, miR-139 was downregulated in cells with iE-DAP-induced EndMT and partly reversed in response to Rg3 via the regulation of NF-κB signaling, suggesting that the Rg3-miR-139-5p-NF-κB axis is a key mediator in iE-DAP-induced EndMT. Conclusion: These results suggest, for the first time, that Rg3 can be used to inhibit inflammation-induced EndMT and may be a novel therapeutic option against EndMT-associated vascular diseases.

• 콘크리트학회논문집
• /
• 제11권4호
• /
• pp.43-54
• /
• 1999

Shear tests are performed on four full-scale 12.5 m proto-type models, "least depth double tee," which are resulted from the optimization process. Domestic superimposed live load regulation, domestic material properties which is available to product. Korean building code requirements, construction environments and economy are considered as the main factors to establish the process. All of the specimens tested fully comply with the shear strength requirements as specified by ACI 318-95. The research has shown following results. 1) The development length requirement of ACI 318-95 does not seem a good predictor for the estimation of bond failure in a beam with the strands below the supports. 2) The load required for the first initial coner cracking in the dap end and first web shear cracking does not seem to have any relation with the dimension and shear strength of the section in the test beams. 3) The strand slip has a direct relationship with the web shear cracking. However, the coner cracking in the dap end does not give any help for the slip in anchorage. 4) Use of whole area for bearing steel at the bottom of dap end is desired for safe bearing pressure design in the precast prestressed double tee beams. 5) The deflection of beam influences directly on the amount of strand slip at the anchorage after initiation of it, and relationship between them are very linear.

• Imaging Science in Dentistry
• /
• 제42권4호
• /
• pp.237-242
• /
• 2012

Purpose: The objectives of this study were to survey the radiographic exposure parameters, to measure the patient doses for intraoral dental radiography nationwide, and thus to establish the diagnostic reference levels (DRLs) in intraoral dental X-ray examination in Korea. Materials and Methods: One hundred two intraoral dental radiographic machines from all regions of South Korea were selected for this study. Radiographic exposure parameters, size of hospital, type of image receptor system, installation duration of machine, and type of dental X-ray machine were documented. Patient entrance doses (PED) and dose-area products (DAP) were measured three times at the end of the exit cone of the X-ray unit with a DAP meter (DIAMENTOR M4-KDK, PTW, Freiburg, Germany) for adult mandibular molar intraoral dental radiography, and corrections were made for room temperature and pressure. Measured PED and DAP were averaged and compared according to the size of hospital, type of image receptor system, installation duration, and type of dental X-ray machine. Results: The mean exposure parameters were 62.6 kVp, 7.9 mA, and 0.5 second for adult mandibular molar intraoral dental radiography. The mean patient dose was 2.11 mGy (PED) and 59.4 $mGycm^2$ (DAP) and the third quartile one 3.07 mGy (PED) and 87.4 $mGycm^2$ (DAP). Doses at university dental hospitals were lower than those at dental clinics (p<0.05). Doses of digital radiography (DR) type were lower than those of film-based type (p<0.05). Conclusion: We recommend 3.1 mGy (PED), 87.4 $mGycm^2$ (DAP) as the DRLs in adult mandibular molar intraoral dental radiography in Korea.

• Restorative Dentistry and Endodontics
• /
• 제44권3호
• /
• pp.25.1-25.10
• /
• 2019

Objective: The aim of this study was to evaluate discoloration of teeth undergoing regenerative endodontic procedures (REPs) using blood clot or platelet-rich fibrin (PRF) as the scaffolds and different calcium silicate-based materials as the intracanal coronal barriers in an ex vivo model. Materials and Methods: Forty-eight bovine incisors were prepared and disinfected using 1 mg/mL double antibiotic paste (DAP). The specimens were then randomly divided into 2 groups (n = 24) according to the scaffolds (blood or PRF). After placement of scaffolds each group was divided into 2 subgroups (n = 12) according to the intracanal coronal barriers (ProRoot MTA or Biodentine). The pulp chamber walls were sealed with dentin bonding agent before placement of DAP and before placement of scaffolds. The color changes (${\Delta}E$) were measured at different steps. The data were analyzed using 2-way analysis of variance. Results: Coronal discoloration induced by DAP was not clinically perceptible (${\Delta}E{\leq}3.3$). Regarding the type of the scaffold, coronal discoloration was significantly higher in blood groups compared with PRF groups at the end of REP and after 1 month (p < 0.05). However, no significant difference was found between PRF and blood clot after 6 months (p > 0.05). Considering the type of intracanal coronal barrier, no significant difference existed between ProRoot MTA and Biodentine (p > 0.05). Conclusions: With sealing the dentinal tubules of pulp chamber with a dentin bonding agent and application of DAP as an intracanal medicament, coronal color change of the teeth following the use of PRF and blood sealed with either ProRoot MTA or Biodentine was not different at 6-month follow-up.

• 한국수산과학회지
• /
• 제39권3호
• /
• pp.297-302
• /
• 2006

황점볼락, Sebastes oblangus의 인공종묘의 성분화 과정 및 성결정을 조사하기 위해 출산 직후부터 370일령까지 조사하였다. 출산 직후 난황기 자어(7.10-7.77 mm)에서 시원생식세 포와 생식융기는 분리된 채 중신관과 장간막 사이에 나타났고, 출산 후 5일령 전기자어(7.12-9.68 mm)에서는 서로 융합하였으며, 출산 후 45일령 후기자어(18.6-20.4 mm)까지는 미분화 생식소 상태였다. 출산 50일 치어(20.0-24.5 mm)에서 생식소 전단부에서 양쪽 끝의 체세포 조직이 분열 신장되어 난소의 분화가 시작되어 60일 치어(25.5-32.0 mm)에서 완전 한 난소강이 형성되었다. 출산 80일령 치어(37.3-47.2 mm)의 난소에서 난원세포가 감수분열을 개시하여 염색인기 난모세포(chromatin nucleolus oocyte) 로 발달하기 시작하였다. 이 후 일령이 증가하여 130일령(68.0-86.0 mm)에서 주변인기(perinucleolus stage)의 난모세포가 나타났으며, 출산 후 370일령(101.0-116.0 mm)에서 전난황형성기의 난모세포들이 출현하였다. 황점볼락 인공종묘의 성비 조사결과 난소분화 완료 후에 암컷만 나타나 출산후 100일까지 자연수온보다 높은 수온에 의해 전암컷화 된 것으로 추정된다.

• 생물환경조절학회지
• /
• 제29권3호
• /
• pp.209-218
• /
• 2020

ET_c 손실을 보상하는데 필요한 물의 양을 작물 용수 요구량(Crop water requirement, CWR)로 정의되며, ET_c 평가는 작물 필요 요구량을 정확하게 정량화하는 데 필요하며, 물 균형 계산에서 중요한 역할을 한다. 토마토와 파프리카의 실제 관수 요구량(Actual crop water, ACW)이 적절한 CWR인지 평가하였다. 토마토와 파프리카 재배에 적정한 AWC 예측 및 추정을 위하여 온실 내부 환경데이터를 Penman-Monteith을 이용하여 기준 작물 증발산(ET)을 계산한 후, 기준 증발산은 작물 상수(Kc;토마토-1.15, 파프리카-1.05)계수로 조정하였다. 토마토와 파프리카의 CWR과 ACW를 계산하여 비교 평가한 결과 ACW가 CWR을 대체할 수 있지만 파프리카의 ACW는 필요 이상으로 높게 나타났다. 또한, 토마토의 ACW는 1일 100 ~ 1,200 ml이고, 파프리카의 ACW는 1일 100 ~ 500 ml가 적절한 것으로 나타났다. 그러나, 스마트 온실에서 ET_c의 정밀도를 높이려면, ET_c가 CWR로 변환되고 ACW와 비교하기 위해서 클래스 A팬 설정이 필요하다. 향후 실시간으로 CWR을 측정하기 위한 시뮬레이션 프로그램 연구가 필요하다.

• 한국보건간호학회지
• /
• 제34권2호
• /
• pp.211-224
• /
• 2020

Purpose: This study was conducted to investigate death attitude, death anxiety, and knowledge toward advance directives of nursing students. Method: Participants were 157 nursing undergraduates in Korea. The students responded to a self-reporting questionnaire that included demographics, Death Attitude Profile-Revised (DAP-R), Revised Death Anxiety Scale (RDAS), and knowledge toward advance directives. The data collection period was December, 9-13, 2019. Data were analyzed by descriptive test, independent t-test, one-way ANOVA, and Pearson's correlation coefficient with SPSS/WIN 23.0. Results: The mean scores for death attitude, death anxiety, and knowledge toward advance directives were 2.70±0.58, 2.45±0.49, and 7.94±2.04, respectively. Knowledge toward advance directives was significantly different according to age, grade, and experience of end-of-life care education. Death attitude was significantly associated with death anxiety (r=. 27, p<.001). Conclusion: Knowledge toward advance directive was relatively low compared to the findings of previous studies. Therefore, nursing colleges need to include legal and institutional aspects when writing written letters on advance directive, death attitude, and ethical approaches to death anxiety.

• 콘크리트학회논문집
• /
• 제11권3호
• /
• pp.141-152
• /
• 1999

Precast prestressed double-tee slab may be designed by the PCI Design Handbook. It is based on the bridge construction and is required for reorganization for the use of buildings in the domestic construction environments. Much enhanced sections are developed from the reforming process on the determined design factors in the previous experimental works on double tees. Pre-determined shape, reinforcement detail, and 5000 psi concrete strength can not be expected as the best solution for the domestic construction requirements because large amount of use on that systems are anticipated. Flexural tests are performed on four full-scale 12.5m proto-type models, "least depth double tee", which are resulted from the optimization process. Domestic superimposed live load regulation, domestic material properties which is available to product, building design requirements and economy in construction are considered as the main factors to establish. the first two sections are double tee section for 1.2 ton/$\m^2$ market live load with straight and one-point depressed strands and the second two are for 0.6 ton/$\m^2$ parking live load with those strand types. All of the specimens tested fully comply with the flexural strength requirements as specified by ACI 318-95. However, the research has shown that following improved considerations are needed for better result in practice. The locations and method of connection for the lowest bottom mild bar, connection method between precast and cast-in-place concrete, and dap-end reinforcement are need to be improved.

• 생물환경조절학회지
• /
• 제18권3호
• /
• pp.258-265
• /
• 2009

본 연구는 현재 대일 수출중인 상업적인 유리온실과 플라스틱 온실에서 파프리카 'Derby'를 공시품종으로 하여 국내 온실 간 생산량 차이 발생을 분석하여, 생산성 차이를 개선하고자 시설내부의 광량, 작물의 생장량 및 수량을 두 온실간 기간별로 비교 분석하였다. 재배기간 동안 평균 일중 광량은 유리온실 $9.03MJ/m^2/day$, 플라스틱 온실 $7.37MJ/m^2/day$로 23%정도 유리온실의 시설내부 광량이 많았다. 총 조사기간 동안 파프리카의 생장량은 유리온실 $1759.9g{\cdot}m^{-2}$, 플라스틱 온실 $1308.5g{\cdot}m^{-2}$으로 유리온실의 작물 생장량이 높았다. 총 건물생산량 대비 영양생장생식생장 기관의 건물분배는 시설내부 광량이 높은 유리온실에서 생식생장 기관의 건물분배는 높았고, 영양생장 기관의 건물분배는 낮았다. 두 온실의 파프리카 생산성은 유리온실 $14.1kg{\cdot}m^{-2}$, 플라스틱 온실 $7.8kg{\cdot}m^{-2}$으로 유리온실이 매우 높은 생산성을 보였다. 본 연구결과는 온실 간 파프리카의 기간별 동적인 건물생산량과 건물 분배 패턴, 수량을 분석함으로써 파프리카의 수량을 예측하고 우리나라 파프리카 생산성 향상을 위한 재배기술 분야의 기초 자료와 농가 간 생산성 차이 원인을 분석하고 그에 따른 생산성 극복 기술을 개발하는데 중요한 자료가 될 것이라고 판단된다.

• Environmental Engineering Research
• /
• 제24권4호
• /
• pp.680-689
• /
• 2019

Temperature effects on the generation of brake wear particles from railway vehicles were generated, with a particular focus on the generation of ultrafine particles. A real scale brake dynamometer test was repeated five times under low and high initial temperatures of brake discs, respectively, to obtain generalized results. Size distributions and temporal patterns of wear particles were analyzed through visualization using clustered heatmaps. Our results indicate that high initial temperature conditions promote the generation of ultrafine particles. While particle concentration peaked within the range of fine sized particles under both low and high initial temperature, an additional peak occurred within the range of ultrafine sized particles only under high initial temperature. The timing of peak occurrence also differed between low and high initial temperature conditions. Under low initial temperature fine sized particles were generated intensively at the latter end of braking, whereas under high initial temperature both fine and ultrafine particles were generated more dispersedly along the braking period. The clustered correlation heatmap divided particle sizes into two groups, within which generation timing and concentration of particles were similar. The cut-off point between the two groups was approximately 100 nm, confirming that the governing mechanisms for the generation of fine particles and ultrafine particles are different.