Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Gonadal Development and Sex Ratio of Artificial Seedlings of the Oblong Rockfish Sebastes oblongus

황점볼락 Sebastes oblongus 인공종묘의 생식소 발달과 성비

  • Kwak Eun-Joo (Wondo Regional Maritime Affairs & Fisheries Office) ;
  • Lee Kyung-Wo (Jeollanamdo Fisheries Research Institute) ;
  • Choi Nak-Hyun (Department of Aquaculture, Chonnam National University) ;
  • Park Chung-Kug (Department of Aquaculture, Chonnam National University) ;
  • Han Kyeong-Ho (Department of Aquaculture, Chonnam National University) ;
  • Lee Won-Kyo (Department of Aquaculture, Chonnam National University) ;
  • Yang Seok-Woo (Mokpo Regional Maritime Affairs & Fisheries Office)
  • 곽은주 (완도수산기술관리소) ;
  • 이경우 (전라남도 수산시험 연구소) ;
  • 최낙현 (전남대학교 해양기술학부 양식생물학) ;
  • 박충국 (전남대학교 해양기술학부 양식생물학) ;
  • 한경호 (전남대학교 해양기술학부 양식생물학) ;
  • 이원교 (전남대학교 해양기술학부 양식생물학) ;
  • Published : 2006.06.01
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Abstract

We investigated gonadal development and sex ratio of artificial seedlings of the oblong rockfish Sebastes oblongus, based on samplings for 370 days just after parturition. The primordial germ cells and genital ridge appeared separately under the mesentery in the yolk-sac stage larva (total length: 7.10-7.77 mm) just after parturition. The primordial germ cells and genital ridge integrated to form primordial gonad in 5-day-old larvae (7.12-9.68 mm), and then proliferation of somatic cell and germ cell occurred in the gonad, which was maintained undifferentiated until 45-days after parturition (18.6-20.4 mm). The ovarian differentiation began in the larva of 50-days old (dab) after parturition (dap) (20.0-24.5 mm). The somatic tissues elongated from the both opposite end-sites of undifferentiated gonad were consequently fused and formed a complete ovarian cavity at 60-days old dap (25.5-32.0 mm). In 80-days old dap (37.3-47.2 mm), meiosis of oogonia occurred to be chromatin nucleolus stage oocyte. The perinucleolus stage oocytes appeared at in 130-days old dap (68.0-86.0 mm), and previtellogenic stage oocytes appeared in 370-days old dap (101.0-116.0 mm). Only female was observed in the artificially produced oblong rockfish in the present study. This result revealed the effect of higher temperature on the sex determination of the oblong rockfish..

황점볼락, Sebastes oblangus의 인공종묘의 성분화 과정 및 성결정을 조사하기 위해 출산 직후부터 370일령까지 조사하였다. 출산 직후 난황기 자어(7.10-7.77 mm)에서 시원생식세 포와 생식융기는 분리된 채 중신관과 장간막 사이에 나타났고, 출산 후 5일령 전기자어(7.12-9.68 mm)에서는 서로 융합하였으며, 출산 후 45일령 후기자어(18.6-20.4 mm)까지는 미분화 생식소 상태였다. 출산 50일 치어(20.0-24.5 mm)에서 생식소 전단부에서 양쪽 끝의 체세포 조직이 분열 신장되어 난소의 분화가 시작되어 60일 치어(25.5-32.0 mm)에서 완전 한 난소강이 형성되었다. 출산 80일령 치어(37.3-47.2 mm)의 난소에서 난원세포가 감수분열을 개시하여 염색인기 난모세포(chromatin nucleolus oocyte) 로 발달하기 시작하였다. 이 후 일령이 증가하여 130일령(68.0-86.0 mm)에서 주변인기(perinucleolus stage)의 난모세포가 나타났으며, 출산 후 370일령(101.0-116.0 mm)에서 전난황형성기의 난모세포들이 출현하였다. 황점볼락 인공종묘의 성비 조사결과 난소분화 완료 후에 암컷만 나타나 출산후 100일까지 자연수온보다 높은 수온에 의해 전암컷화 된 것으로 추정된다.

Keywords

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