• 한국어병학회지
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• 제1권2호
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• pp.111-116
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• 1988

There occurred a large quantity of death of the cultured carp (Cyprinus carpio) in net pens in Lake Taechong and Lake Chungju(Chungcheongbuk-do) from July through Sep., 1987. The carp were two-year-old and 500g to 2,500g in body weight. As a result of biopsy of dying carp, 1 through 10 egg-shaped giant cysts with 12 through 60mm in diameter were observed in their intestine per one carp. The author suggested that the carp were died from blockade of intestinal tract and following enteritis by the giant cysts. The giant cysts were filled with numerous mature Thelohanellus genus spores confirmed to Thelohanellus kitauei after following examination. In this case the author could find out this disease was spread by transverse infection following infestation in net pens of fingerling fish farms.

• Clinical and Experimental Reproductive Medicine
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• 제27권4호
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• pp.405-415
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• 2000

Objective: The present study was undertaken to synthesize a human Y chromosome specific probe and to confirm the usefulness of the probe for fluorescence in situ hybridization (FISH) in various types of human cells. Methods: An approximately 400 bp DNA fragment of the DYZ1 sequences was synthesized by PCR using digoxigenin labeled dUTP (dig-PCR). The fidelity of probe was tested by FISH for cultured and uncultured human lymphocytes, amniocytes, chorionic villus cells, embryos, sperms, and germ cells of seminiferous tubule. Results: The human Y chromosome specific probe hybridized specifically to Y chromosome of the cells that had been tested. This probe assigned to the Yq12 region where the DYZ1 repetitive sequence is concentrated. Conclusion: We have identified a human Y chromosome specific probe that hybridized specifically to the Y chromosome by FISH for various types of uncultured as well as cultured cells. Therefore FISH technique using human Y chromosome specific probe should be useful for clinical application as a diagnostic tool for the detection of human Y chromosome.

• 한국양식학회지
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• 제19권1호
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• pp.14-18
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• 2006

해산어류인 감성돔의 담수사육에 따른 어육을 평가하기 위하여 근육의 경도와 강도를 조사하였으며, 설문조사를 통한 어육의 외관, 질감, 맛 및 냄새를 분석하였다. 또한 수익성분석을 위해 치어 및 육성어를 10개월간 양식하여 각각의 순수익(률)을 산출하였다. 담수사육 감성돔 근육의 경도 및 강도는 각각 $9210{\pm}1215g/cm^2,\;30693{\pm}6355g/cm^2$로 해수사육의 $9987{\pm}1893g/cm^2,\;31931{\pm}6549g/cm^2$보다 다소 낮았으나 유의한 차이는 없었다. 또한 설문조사한 결과, 어육의 외관, 질감, 맛 및 냄새에서도 담수 및 해수사육한 감성돔사이에 차이가 없는 것으로 나타났다. 감성돔 치어 및 육성어를 담수에서 10개월간 양식하였을 때 순수익(률)은 각각 24,000,000원(30.0%), 53,870,000원(36.9%)으로 나타났다.

• 한국어병학회지
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• 제24권2호
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• pp.103-112
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• 2011

본 연구는 실험을 위해 저수온에서 순치 중이던 쥐치에서 체표의 백탁을 보이며 폐사하여 그 원인을 밝히고자 하였다. 순수 배양된 균체를 확인하여 계대 배양하여 생화학적 성상과 16S rRNA유전자와 recA 유전자의 염기서열 분석 결과 Vibrio anguillarum으로 나타났다. 빈사어 (체장 4~6 cm)의 약 30%는 체표 백탁 증상을 보였으며 약 10%는 폐사하였다. 이에 약제 감수성 시험 후, oxolinic acid 200 ppm을 1시간 동안 약욕시켰으며, 2일 경과 후 폐사가 중단되고 증상이 호전되었다.

• Fisheries and Aquatic Sciences
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• 제14권4호
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• pp.379-388
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• 2011

The mechanism by which gonadotropin-releasing hormone (GnRH) and dopamine (DA) control gonadotropin (GTH) release was studied in male and female rainbow trout using cultured pituitary cells obtained at different reproductive stages. The mechanisms of follicle-stimulating hormone (FSH) release by GnRH and DA could not be determined yet. However, basal and salmon-type GnRH (sGnRH)- or chicken-II-type GnRH (cGnRH-II)- induced luteinizing hormone (LH) release increased with gonadal maturation in both sexes. LH release activity was higher after sGnRH stimulation than cGnRH-II stimulation at maturing stages in both sexes. The GnRH antagonist ([Ac-3, 4-dehydro-$Pro^1$, D-p-F-$Phe^2$, D-$Trp^{3,6}$] GnRH) suppressed LH release by sGnRH stimulation in a dose-dependent manner, although the effect was weak in maturing fish. The role of DA as a GTH-release inhibitory factor differs during the reproductive cycle: the inhibition of sGnRH-stimulated LH release by DA was stronger in immature fish than in maturing, ovulating, or spermiated fish. DA did not completely inhibit sGnRH-stimulated LH release, and DA alone did not alter basal LH release. Relatively high doses ($10^{-6}$ or $10^{-5}M$) of domperidone (DOM, a DA D2 antagonist) increased LH release, which did not change with reproductive stage in either sex. The potency of DOM to enhance sGnRH-stimulated LH release was higher in maturing and ovulated fish than in immature fish. These data suggest that LH release from the pituitary gland is controlled by dual neuroendocrine mechanisms by GnRH and DA in rainbow trout, as has been reported in other teleosts. The mechanism of control of FSH release, however, remains unknown.

• 한국어병학회지
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• 제17권1호
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• pp.21-28
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• 2004

양식 미꾸라지 (Misgurnus mizolepis)가 수중으로 잠입하지 못하고 양어지의 물표면에 배를 위로 한 채 힘없이 회전 유영을 하는 질병이 발생하였다. 병어는 복부가 팽만되어 있었으며, 해부하여 보면 소화관에 커다란 공기방울의 축적, 장염이 현저하였다. 병어를 혈액학적으로 조사한 결과 적혈구수, Ht치 및 Hb 농도는 건강어와 차이가 없었다. 병리조직학적으로는 아가미의 호흡상피의 증생과 새변의 유착, 소화관 상피세포의 출혈, 괴사 및 박리가 관찰되었다. 뒷지느러미와 항문사이를 실로 결박하여 항문으로부터 공기의 배출을 억제한 재현 실험에서 발병어와 같은 증상이 재현되었다. 따라서 이 병은 빠른 성장을 목적으로 고단백질 사료를 투여함으로서 장호흡을 위하여 마신 공기가 소화관내에 축적되고, 축적된 공기의 부력 때문에 물속으로 하강하지 못하게 되고, 또 소화관을 압박하여 소화관에 순환장애를 일으킨 결과 폐사한 것으로 추정된다.

• 한국어병학회지
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• 제37권1호
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• pp.97-110
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• 2024

The Serranidae is high-quality fish species with good meat quality and is traded at high price, and is attracting attention in South Korea as a cultured species that creates high added value. However, the high-density fish farming for mass production increases the risk of mass mortality due to infectious diseases, leading to enormous economic losses. Therefore, in order to safely prevent and protect farmed fish from serious infectious diseases, it is necessary to conduct disease monitoring on a regular basis. In this study, Hyporthodus septemfasciatus, Epinephelus moara, and the hybrid longtooth grouper (E. moara ♀×E. lanceolatus ♂) were collected once a month from fish farm of Garorim and Aquabiotech Co., Ltd for a total of six months, from July to December 2023. We investigated infections of five species of bacterial diseases, including Flavobacterium columnare, six species of viral diseases, including LCDV (lymphocystis disease virus), and parasitic pathogens in grouper farms. As the result, Vibrio vulnificus and V. harveyi were detected in H. septemfasciatus in August, in the case of viral diseases, NNV was detected in H. septemfasciatus from July to August using RT-PCR or PCR. Finally, In the case of parasitic diseases, Tricodina sp. was detected in E. moara and the hybrid longtooth grouper from August to December.

• 수산경영론집
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• 제48권2호
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• pp.53-66
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• 2017

More than 90% of cultured fish is consumed by sliced raw fish which is usually an eating out menu in South Korea. In order to develop the aquaculture industry in Korea, It is very important to know whether consumers can differentiate each species or not and how consumers recognize each species by certain criteria. for example taste, seasonal preference. The purpose of this study is to understand the competitive relationship through positioning studies of each species by the selection attributes. A total of 221 consumers were surveyed in korea. This study adapted multidimensional scaling(MDS) analysis to explore how consumers position sliced raw fish species based on selection attributes. This study has produced perceptual maps of sliced raw fish market. Empirical data was collected from sliced raw fish consumers in Korea. The results of MDS analysis reveal that 7 species are divided into 3 groups(flat fish, black rock fish), (red sea bream, salmon, tuna), (sea bass, gray mullet). In this study flat fish and black rock fish are perceived as safe, familiar, good value species. Red seabream, salmon, tuna are perceived as luxurious species. Sea bass and gray mullet are perceived as unfamiliar species.

• 한국식품과학회지
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• 제41권1호
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• pp.42-49
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• 2009

본 연구는 건조 배합사료를 공급한 양식 넙치와 생사료를 공급한 양식넙치의 품질특성을 비교해 보고자 하였다. 즉, 건조 배합사료로서는 시중에 판매되고 있는 상품사료(CEP)와 직접 설계 제조한 실험사료(FEP) 2종을 사용하였고 생사료(MP)를 대조구로하여, 약 10달간 각각의 사료를 공급한 넙치(1.15 kg)의 등근육 및 지느러미 살을 대상으로 하여 각 사료 공급에 의한 일반성분, 지방산, 아미노산, 관능평가 및 물성측정을 통한 어육의 품질차이를 비교하였다. 넙치 등근육의 일반성분의 각 함량은 차이가 없었으나, 지느러미 살의 경우는 MP 공급구가 수분함량이 높고 조지방 함량은 낮았다. 넙치 어육의 지방산 조성은, 넙치를 사육하는 동안 공급하는 사료의 종류에 따라 등근육 및 지느러미살의 지방산 조성은 차이가 있음을 알 수 있었고, 주로 축산육의 품질 측정시 지표로 사용되던 18 : 0/18 : 2, 18 : 2/18 : 3 및 n-6/n-3과 같은 지방산의 비율을 활용해서 어류의 풍미와 정미성을 비롯해 어육의 질감 차이까지도 예측할 수 있을 것으로 사료되었다. 또한, MP, CEP 및 FEP를 공급한 넙치 등근육의 구성아미노산 조성 중 EAA, FAA, SAAA, SAA 및 FRAA의 함량에는 차이가 없었으나, 지느러미살의 경우는 맛 관련 성분인 glutamic acid와 감미성분인 glycine 및 alanine이 각 사료구별로 유의적인 차이를 나타내었다. 냄새, 외관, 맛, 질감 및 종합적인 기호도면에 대해 관능검사를 실시한 결과 모든 사료구에서 유의적인 차이는 없었고, 경도(hardness)는 MP 공급구가 CEP 및 FEP 공급구보다 높았다. 물성측정 결과도 역시 모든 사료구에서 유의적인 차이는 없었다. 본 실험 결과로부터 건조 배합사료를 공급한 넙치의 등근육 및 지느러미 살의 품질 특성은 생사료를 공급한 넙치와 큰 차이는 없음을 알 수 있었다.

• Ocean and Polar Research
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• 제26권3호
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• pp.415-423
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• 2004

An experiment was conducted to investigate the differences of total ammonia nitrogen (TAN) excretion rates and feces production rates among the offsprings from cultured Japanese strain (JJ, mean BW; $17.1{\pm}0.1g$), intraspecific hybrid strain between cultured Japanese and selected Korean strain (JK, mean BW: $17.1{\pm}0.1g$) and selected Korean strain(KK, mean BW: $21.5{\pm}0.1g$) of red sea bream in order to compare their dietary protein utilization efficiency. Fish were hand-fed with a commercial diet containing 46.7% crude protein for 2 weeks, three times daily 09:00, 13:00 and 17:00. After daily feeding, the TAN excretion rates reached peaks of 49.03, 58.75 and 36.26mg/kg fish/hr for the JJ, JK and KK strain, respectively, during the daytime. The value of the KK strain was significantly lower than that of the JJ and JX shuin (P<0.05), however daily TAN excretion rates of the JJ, JK and KK strain were not different (P>0.05). When fish were fed at satiation after 4 days of starvation, TAN excretion rates reached the maximum values 4 hours after the feeding fur the KK (31.23 mg/kg fish/hr) and 6 hours after the feeding fur the JJ (44.19 mg/tg fish/hr) and JK strain (41.70 mg/kg fish/hr). After 3 days of starvation, the daily endogenous TAU excretion rates (ETE) for the JJ, JK. and KK strain were 286.91, 215.66 and 179.29mg/kg fish/day, respectively. The value of the KK strain was lower than that of the JJ and JK strain (P<0.05). The total feces production rates of the JJ, JK and KK strain were not significantly different, however the proportions of feces production rates by time for the JJ, JK and KK strain were different (P<0.05). As overall results, efficiency of dietary protein utilization of JJ, JK and KK seems to be different and KK strain could offer a desirable option for aquaculture purpose.