The present study was performed to improve the technique used for fermenting the mushroom growth medium. Taxonomic analysis of 16S rDNA sequence from the predominant Bacillus strain CY-24 isolated during the fermentation phase of the rice straw medium identified it as Bacillus licheniformis. In addition, the growth environment of B. licheniformis was also examined in this study, which revealed the optimal growth temperature and pH to be 30 ℃ and 6.0, respectively. This study also revealed that carboxymethyl cellulase (CMCase) and polygalacturonase (PGase) enzymes isolated from B. licheniformis achieved their maximal activities at 50 ℃ and 60 ℃ respectively. Furthermore, the study confirmed that the two enzymes, i.e., CMCase and PGase in B. licheniformis are stable at temperatures above 60 ℃. The present study thus demonstrates that B. licheniformis CY-24 possesses excellent enzymatic properties. It also reveals that the action of enzymes during the production of growth mediums used for the cultivation of mushrooms is closely associated with the promotion of fermentation and softening of the rice straw. Overall, this study provides elementary information regarding the role of B. licheniformis enzymes during growth medium fermentation for Agaricus bisporus cultivation.
Yeon Bok Kim;Kwang Jin Chang;Chang Ha Park;Sang Un Park
Journal of Practical Agriculture & Fisheries Research
/
v.25
no.2
/
pp.5-11
/
2023
Rehmannia glutinosa is a perennial herb belonging to the family Scrophulariaceae and is used in traditional oriental medicine. This study was carried out current status of breeding of R. glutinosa, its problems, and the matters to be improved were made. Based on the research data published so far, this study summarized the characteristics of breeds cultivated in Korea and compared and analyzed the status of cultivation area, production, import, and price changes for 15 years from 2008 to 2021. The cultivated area of R. glutinosa is gradually decreasing, and the amount of import is increasing and decreasing repeatedly. Amount of import has declined significantly. There was a lot of disinfection and disposal in the middle of import, and dried Jihwang and Sukjihwang were imported without distinction. R. glutinosa must be cultivated and supplied as a variety desired by the industry, cultivation techniques need to be taught to producers. In order to clearly identify the amount of import, import code of dried Jihwang and Sukjihwang must be classify. In addition, it is necessary to develop and supply harvesting agricultural machines for mechanization. For the stable income of producers, supply control through contract cultivation will ensure stable farm income.
The rice culture techniques included 'Jodosukyungbeob'(旱稻水耕法 : culture techniques of early-ripening paddy rice), 'Mandosukyungbeob' (晩稻水耕法) : culture techniques of late-Ripening paddy rice 'Handobeob'(旱稻<山稻>法 : culture techniques of upland rice), 'Myojongbeob'(苗種法 : culture techniques of paddy rice by transplanting), 'Kunangbeob'(乾秧法 : culture techniques of rice by transplanting which rears seeding in dry paddy) and 'Sudogunpanongbeob'(水稻乾播農法 : culture techniques of paddy rice seeding in dry field). Especially, 'Kunangbeob' and 'Sudogunpanongbeob' were originally developed in Korea as seen in 1600s(Kyoungje : 經濟) and early 1800s (Yoji : 要旨). In 'Jodosukyungbeob' it took 9 days for seed dipping, water-sprouting and prevent damage by birds, each for 3 days in China, but in Korea seed dipping in water took 3 days and the rest of the procedures were flexibly established. In matured soils, practices were fall plowing right after harvest, recognition of effective tillering and additional fertilization use of human manure, and stimulation of sprouting by lime application. The unique culture techniques adequate for Korean situations were practiced, which included weed control after draining accurately for 3 to 4 times, draining at mid season for improving wind and drought tolerance, rice harvesting at appropriate time for preventing grain shattering, and seeding in rows. 'Mandosukyungbeob' was improved techniques contrast to those of China, and the major contents were selection of proper varieties, good stand establishment by seeding high rates, induction of vigorous tillers, and adoption of 'Jokjongbeob'(足種法 : seeding method by foot). Also, one of the most prominent rice cultures by our ancestors was 'Kunpanongbeob' that was systemized form habitual practice of Pyongan Province. The unique technique actualized was 'Hando [旱稻(山稻)]' culture technique which was the combinations of 'Jokjongbeob', root stimulation method, and disaster-tolerant mixture cropping with adoptation of variety theory, although it was originated from China. The transplanting techniques has come before 'Jikseol'($\ulcorner$直說$\lrcorner$) and its merits were sufficiently realized. However, this method was basically prohibited from the early Chosun dynasty because extremely bad harvest was expected under drought conditions and insufficient conditions of water storage. But, it was permitted in the areas that contained water all the times and in case of large-scale farming especially. Most of rice culture was transplanted in the end of the Chosun dynasty because transplanting was continuously spreaded in the three southern provinces of Korea. Under these circumstances, transplanting technique was improved from the early to the end of the Chosun dynasty by weed control, fertilizing, water management, and quadratic transplanting. Based on these techniques, agricultural productivity was improved 5 times by that time. 'Kunpanongbeob' was created and developed properly for Korean conditions that is dry in early season and flooding in late season. This was successively developed and established into transplanting technique of nursery seedling.
Yeon-Am's observation of natural objects will be able to contribute to the cultivation of the observation skills. In this study, I analysed Yeon-Am's observation skills in the book "Yorha Ilgi". Yeon-Am's observation skills are as follows. First, Yeon-Am observed a specific and detailed. Second, Yeon-Am was examined by utilizing the five senses and presented its risks and limitations. Third, Yeon-Am observed with the equality between man and nature and looked at the same level of human and nature. Fourth, Yeon-Am expressed those observed by utilizing the shaping techniques vividly. Fifth, Yeon-Am found a variety of features by a variety of perspectives. The orgin of Yeon-Am's writing is based on specific and detailed observations of the natural objects, joining nature, shaping techniques and diversity in perspective.
Journal of the Korean association of regional geographers
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v.18
no.1
/
pp.101-117
/
2012
The purpose of this research is to propose a plan on migration policy based on features, through analyzing the migration adaption process and the farming life of the back-to-countryside population throughout the nation. For this, survey has been conducted to find out urban-to-rural migrants' motives and characteristics. The major reason for the migration was the household's financial condition, and the most of migration decisions were made by either one's spouse or parents. The migration fund was primarily spent in early agricultural activities and it was the neighbors who aided them the most whilst teaching them many farming techniques. Some government-supported policies are being highly demanded regarding the lower level of income (than urban life) and difficulties of raising cultivation funds; these two things are what migrants consider as the most inconvenient and suffering matters in farming life. Many surveyee have responded that they would constantly stay in the rural area and continue on their life as a farmer, giving a promising prospect for future farmland life. Based on these trends and features, development strategy regarding elaborate settlement plan of urban-to-rural migration is necessary.
A wide variety of pathogens are known io be seedborne, carried either as infectious mycelium internally or as contaminants on the seed coat. When seed is infected with a pathogen, the seed nay be rendered nonviable or it may remain viable but produce weak seedling. In some cases, the Infected seedling nay not be severely weakened, but nay serve as a source of primary inoculum within a community of plants. A recent problem nay be the dissemination of seedborne pathogens occurring as a result of the massive movements of seed, as a part of the 'Green revolution' Disease of great danger to agriculture may be introduced with seed from other parts of world. Seed treatment with organic mercury compounds in liquid form had become popular since about 1955. Organic mercury compounds contributed considerably to the increase in production of many crops and vegetables. In 1975, however, the use of organic mercury compound was forbidden because of doubts regarding their residual mammalian toxicity in agricultural products. Benomyl-thiram mixture, thiophanate methyl-;hiram mixture and TCMB have now been registered as seed disinfectants for the use of rice blast, brown spot and Bakanae disease. Oxathiinsthiram mixture has been registered as seed disinfectant for barley and wheat loose smut and leaf stripe of barley. Agricultural techniques have made such rapid progress that the nursery methods changed from the use of paddy nursery to box nursery designed for machine-transplanting. The spread of rice transplanting machines has caused increase of seedborne diseases. Among seedborne diseases, Bakanae disease has remarkably increased and causes much damage recently. In order to counter this trend, seed disinfectants must also be diversified. First, effective non-selective disinfectants need to be developed, and second, appropriate control methods always need to be prepared in parallel with the development of new techniques for cultivation.
This study was carried out to improve cultivation techniques by low cost and labour saving in ginseng (Panax ginseng C. A. Meyer) seedling production, by elucidating proper ratio virgin soil and organic fertilizer, suitable height of front piller. The obtained results are as follows ; The optimal ratio of white decomposition of virgin soil and organic fertilizer was 10:1 for good yield of standard seedling, The fittest height of front piller was 150 cm because of both good seedling growth and yield. The cost for production of seedling of ginseng could be reduced by both optimal ratio of virgin soil, organic fertilizer and selection of front piller height.
The purpose of this study is to help self-healing by identifying self-description by utilizing the self-description diagnosis of literary therapy and the resonance of the work narrative. Therefore, the research was conducted through the methodology of literature therapy, which uses the self-introduction team of literary therapy and basic photography team to understand their own narrative and to heal themselves through resonance with the work narrative. Through this study, we understood that it is possible to understand and identify and understand one's psychological problems through self-description and self-culture techniques. This case study revealed that it can be used as a new technique for understanding self-description by applying the methodology of literary therapy and for healing self-exploration through resonance with the work narrative.
This study was conducted to supplement new-normal strategies on management plans of rural groundwater resources in the era of Post COVID-19. Global outbreak of COVID-19 has damaged across all areas including public policy, economics, industrial services, and others without exception, which has resulted in establishing new-normal strategies in order to restore balance and functions as for these areas. The new-normal ones were represented as enhancing preventative management on infectious diseases, expanding non face-to-face services, enhancing protective trades and food securities, and preparing growth policies on public services using the 4th industrial revolution techniques. In this study, G-WASH_AD (Groundwater supply, sanitation, and hygiene with attraction and digitization) was suggested to be new-normal strategies on rural groundwater resources. The G-WASH_AD was consisted of three detailed action plans: a preventative plan on waterborne-diseases of groundwater (PP), a groundwater-tourism plan with rural heritage (GP), and an application plan of the 4th industrial revolution techniques to groundwater facilities and its data (P4). The PP can contribute to protect human health from waterborne-diseases and minimize hazardous effects on crop cultivation. The GP accompanied with high-quality groundwater resources is able to strengthen rural tourism, to promote marketing activities on local agricultural products, and to increase household incomes of rural communities. The P4 can reinforce fast, comfortable, and scientific management on groundwater facilities and its data, creating a virtuous cycle between innovative management on groundwater and growth of technology related to it. Results of the G-WASH_AD strategies can encourage a green growth engine in field of rural groundwater management keeping up with Post COVID-19.
Jiksing, Calvin;Ongkudon, McMarshall M.;Thien, Vun Yee;Rodrigues, Kenneth Francis;Yong, Wilson Thau Lym
ALGAE
/
v.37
no.2
/
pp.105-121
/
2022
Modern seaweed farming relies heavily on seedlings from natural beds or vegetative cuttings from previous harvests. However, this farming method has some disadvantages, such as physiological variation in the seed stock and decreased genetic variability, which reduces the growth rate, carrageenan yield, and gel strength of the seaweeds. A new method of seedling production that is sustainable, scalable, and produces a large number of high-quality plantlets is needed to support the seaweed farming industry. Recent use of tissue culture and micropropagation techniques in eucheumatoid seaweed production has yielded promising results in increasing seed supply and growing uniform seedlings in large numbers in a shorter time. Several seaweed species have been successfully cultured and regenerated into new plantlets in laboratories using direct regeneration, callus culture, and protoplast culture. The use of biostimulants and plant growth regulators in culture media increases the seedling quality even further. Seedlings produced by micropropagation grew faster and had better biochemical properties than conventionally cultivated seedlings. Before being transferred to a land-based grow-out system or ocean nets for farming, tissue-cultured seedlings were recommended to undergo an acclimatization process to increase their survival rate. Regular monitoring is needed to prevent disease and pest infestations and grazing by herbivorous fish and turtles during the farming process. The current review discusses recent techniques for producing eucheumatoid and other valuable seaweed farming materials, emphasizing the efficiency of micropropagation and the transition from laboratory culture to cultivation in land-based or open-sea grow-out systems to elucidate optimal conditions for sustainable seaweed production.
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