• Journal of Mushroom
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• v.16 no.2
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• pp.125-129
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• 2018

In order to develop a smart cultivation facility based on ICT (Information Communication Technology), a cultivation house was selected. Sensor devices were installed to monitor any changes in the cultivation environment. A control panel was constructed to monitor and control the data on environmental changes collected by the sensors. To efficiently manage the proceedings of the cultivation environment, the cultivation process was divided into 4 stages. We designed an environmental control module using these processes. PC and mobile phone software were designed for remote monitoring and control to develop a smart cultivation system that can conveniently manage the cultivation environment and produce mushrooms in a more stable manner.

• Journal of Mushroom
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• v.19 no.3
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• pp.251-255
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• 2021

The competitiveness of the mushroom industry can be increased by diversifying the domestic Lentinula edodes cultivar. Therefore, 'Chungheung 1ho' was cultivated by hybrid breeding method using strain 'E140025,' which was collected by the Chungheung Mushroom Farming Association. Regarding the morphological characteristics of the fruit, the cap of 'Chungheung 1ho' was smaller than that of the control cultivar, whereas, its stem was longer and thicker than that of the control cultivar. In addition, the weight of the individual fruits of the new cultivar was heavier than that of the control cultivar. The period for primordia formation was 24 days for the new cultivar, which was 16-20 days later than that of the control cultivar. Furthermore, it was found that the patterns of the PCR amplification band using six URP primers were different for 'Chungheung 1ho' than those of the mating strains.

• Journal of Mushroom
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• v.13 no.4
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• pp.294-300
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• 2015

We cultivated 117 hybrid strains of Lentinula edodes in greenhouse. 5 hybrid strains(07-55, 07-66, 07-84, 07-93 and 07-117) were selected from comparison of their characteristics of fruiting body and productivity. Biological efficiency of 5 hybrid strains was investigated 36.1(07-66), 35.6(07-117), 27.1(07-93), 25.7(07-84) and 6.5%(07-55), respectively. Selected strains 07-66, 07-117 and 07-93 showed high biological efficiency but observed commercially low quality fruiting body. Temperature type of fruiting body of 5 hybrid strains observed as follows high temperature type(07-117), mid temperature type(07-66 and 07-84) and low temperature type(07-55 and 07-93). Temperature type of fruiting body of 07-93 strain has wide range relatively. In the results, 07-84 strain was selected finally with consideration for quality of fruiting body, productivity and temperature type($8-21^{\circ}C$) in farm test.

• Journal of Mushroom
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• v.11 no.4
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• pp.226-229
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• 2013

This study was carried out to elucidate suitable wavelength of light during development of fruit body in Lentinula edodes. The four colors of LED(Light Emitting Diode), blue, green, red and yellow, were irradiated for formation of fruit-body. The effect of color of LED at all growth stage, the lightness of cap showed darker in blue and green LED irradiation than that of red and yellow LED. The longer stipe were resulted in longer wavelength. And the activity of anti-oxidant did not showed big differences according to LED wavelength. We obtained higher commercial yields and lower ratio of abnormal fruit body in green LED than those in control(fluorescent lamp).

• Journal of Environmental Science International
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• v.28 no.4
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• pp.435-439
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• 2019

This study was carried out to investigate the changes in the amount of 20 free amino acids in PDB (Potato Dextrose Broth) medium during the incubation period while cultivating Lentinula edodes. The total incubation period was 90 days, and the total amount of free amino acids was confirmed every 45 days. Among the 18 kinds of amino acids whose increase and decrease patterns were confirmed, 10 amino acids were increased compared to that in the control, among which cysteine increased from its initial value of $9,889{\pm}3{\mu}g/L$ to $12,909{\pm}2{\mu}g/L$ at 45 days and $29,256{\pm}4{\mu}g/L$ at 90 days. Six amino acids with decreased expression patterns were identified. Arginine decreased to $83,751{\pm}2{\mu}g/L$ after 45 days from its initial value of $161,787{\pm}1{\mu}g/L$ and to $79,055{\pm}7{\mu}g/L$ at 90 days.

• The Plant Pathology Journal
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• v.33 no.3
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• pp.264-275
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• 2017

The spent mushroom substrate (SMS) of Lentinula edodes that was derived from sawdust bag cultivation was used as materials for controlling Phytophthora blight disease of pepper. Water extract from SMS (WESMS) of L. edodes inhibited mycelial growth of Phytophthora capsici, suppressed Phytophthora blight disease of pepper seedlings by 65% and promoted growth of the plant over 30%. In high performance liquid chromatography (HPLC) analysis, oxalic acid was detected as the main organic acid compound in WESMS and inhibited the fungal mycelium at a minimum concentration of 200 mg/l. In quantitative real-time PCR, the transcriptional expression of CaBPR1 (PR protein 1), CaBGLU (${\beta}$-1,3-glucanase), CaPR-4 (PR protein 4), and CaPR-10 (PR protein 10) were significantly enhanced on WESMS and DL-${\beta}$-aminobutyric acid (BABA) treated pepper leaves. In addition, the salicylic acid content was also increased 4 to 6 folds in the WESMS and BABA treated pepper leaves compared to water treated leaf sample. These findings suggest that WESMS of L. edodes suppress Phytophthora blight disease of pepper through multiple effects including antifungal activity, plant growth promotion, and defense gene induction.

• The Korean Journal of Mycology
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• v.44 no.4
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• pp.296-299
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• 2016

Lentinula edodes is an edible mushroom that contains a ${\beta}$-glucan called lentinan, which has antitumor and immune-enhancing properties. In the present study, the ${\beta}$-glucan contents of L. edodes mushrooms cultivated on sawdust with different nutritional supplements and fruiting temperatures were measured using a commercial ${\beta}$-glucan assay kit purchased from Megazyme (Bray, Ireland). The weight loss of sawdust media and the yield of fruiting bodies showed similar trends, but the yield was more closely associated with the nutritional supplements used than the weight loss of sawdust media was. The ${\beta}$-glucan contents of L. edodes were 39.5-42.1%, except in the bean curd refuse + $CaCl_2$ supplementation group (50.4%). Furthermore, the ${\beta}$-glucan content decreased with increasing temperatures and was 42.4% at a low fruiting temperature.

• The Korean Journal of Mycology
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• v.44 no.2
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• pp.100-102
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• 2016

Ergothioneine is a natural compound with strong antioxidant properties. In this study, the effects of different nitrogen sources including ammonium nitrate, ammonium sulfate, sodium nitrate, and histidine in sawdust media were investigated to enhance ergothioneine contents in Shiitake (Lentinula edodes) fruit bodies. The addition of 0.2% ammonium sulfate in the growth media showed the highest enhancement of ergothioneine content in shiitake fruit bodies which was 1.7-fold higher than the control. On the other hand, histidine, a building block of ergothioneine decreased the concentration of ergothioneine significantly. Our results demonstrate that the cultivation of shiitake in sawdust media with suitable nutrients was effective to enhance its ergothioneine contents.

• The Korean Journal of Mycology
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• v.50 no.4
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• pp.301-310
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• 2022

Lentinula edodes is a type of mushroom widely consumed in East Asia, and many of the components contained in them are used as raw materials for pharmaceuticals or as health supplements. Ergothioneine, a sulfur-containing amino acid in L. edodes, is attracting attention due to its antioxidant and anti-inflammatory effects. L. edodes generally contain an ergothioneine content of about 200 to 800 mg per kg; there are some differences in this value depending on the cultivation conditions. In this study, 24 domestic sawdust-cultivated L. edodes cultivars were cultured under the same conditions, and the morphological characteristics and ergothioneine content of the fruiting bodies were investigated. The yield and morphological characteristics of the fruiting bodies were different in each cultivar. Taehyanggo and Sanjo 713ho had the largest and thickest pileus. The pileus of L. edodes had a higher ergothioneine content than the stipe. Sanjo 715ho had the highest ergothioneine content in the pileus and the stipe at 1,225 mg/kg and 753 mg/kg, respectively. An ergothioneine content of more than 1,000 mg/kg in the pileus was observed in Bambithyang, Sansanhyang, Sulbaekhyang, Taehyanggo, Sanjo 705ho, Sanjo 709ho, Sanjo 715ho, and Sanjo 716ho. These cultivars can be used as parental strains to develop new cultivars with enhanced ergothioneine functionalities.

• Journal of Mushroom
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• v.16 no.2
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• pp.79-85
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• 2018

This study was carried out to separately assess the production of post-culture, germinating, growing, and resting stages of Lentinula edodes grown in a smart cultivation facility based on ICT technology. The cultivation environment of the greenhouse facility was controlled to remain at different set points throughout a range of high ambient temperatures. Production of Sanjo-701-ho, which is a typical summer cultivar, and the new cultivar Sanjo-701-ho were tested in this ICT-based smart cultivation system. Sanjo-701-ho produced 353.7 g/bag of mushrooms from June to October, while Sanjo-701-ho produced 270.4 g/bag. These production amounts were stable over the high-temperature range of $20-35^{\circ}C$.