DOI QR코드

DOI QR Code

Changes of Free Amino acid in PDB Medium by Lentinula edodes (Sanjo 701ho) Cultivation period

표고버섯 균사체 배양기간에 따른 PDB 배지 내 유리 아미노산 변화

  • Oh, Tae-Seok (Department of plant Resources, Kongju National University) ;
  • Park, Youn-Jin (Green Manure and Legumes Resource Center, Kongju National University) ;
  • Kim, Tae-Kwon (Green Manure and Legumes Resource Center, Kongju National University) ;
  • Ann, Seoung-Won (Department of Horticultural Science, Kongju National University) ;
  • Kim, Chang-Ho (Department of plant Resources, Kongju National University) ;
  • Cho, Yong-Koo (Department of plant Resources, Kongju National University) ;
  • Jang, Myoung-Jun (Department of plant Resources, Kongju National University)
  • 오태석 (공주대학교 식물자원학과) ;
  • 박윤진 (공주대학교 두과녹비자원연구센터) ;
  • 김태권 (공주대학교 두과녹비자원연구센터) ;
  • 안승원 (공주대학교 원예학과) ;
  • 김창호 (공주대학교 식물자원학과) ;
  • 조용구 (공주대학교 식물자원학과) ;
  • 장명준 (공주대학교 식물자원학과)
  • Received : 2019.03.22
  • Accepted : 2019.04.15
  • Published : 2019.04.30

Abstract

This study was carried out to investigate the changes in the amount of 20 free amino acids in PDB (Potato Dextrose Broth) medium during the incubation period while cultivating Lentinula edodes. The total incubation period was 90 days, and the total amount of free amino acids was confirmed every 45 days. Among the 18 kinds of amino acids whose increase and decrease patterns were confirmed, 10 amino acids were increased compared to that in the control, among which cysteine increased from its initial value of $9,889{\pm}3{\mu}g/L$ to $12,909{\pm}2{\mu}g/L$ at 45 days and $29,256{\pm}4{\mu}g/L$ at 90 days. Six amino acids with decreased expression patterns were identified. Arginine decreased to $83,751{\pm}2{\mu}g/L$ after 45 days from its initial value of $161,787{\pm}1{\mu}g/L$ and to $79,055{\pm}7{\mu}g/L$ at 90 days.

Keywords

References

  1. Aminiddin, H., Mohd Khan, A., Abidin, H., Madzlan, K., Suri, R., Kamal, M. K., Nor Ajila, S., 2007, Effect of different rates of agitation on mycelium growth and amino acid composition of Lentinula edodes in submerged cultures, Journal of Tropical Agriculture and Food Science, 35, 139-146.
  2. Breene, W., 1990, Nutritional and medicinal value of specialty mushrooms, Journal of Food Protection, 53, 883-894. https://doi.org/10.4315/0362-028X-53.10.883
  3. Cho, D. B., Hyun, K. H., Choi, J. H., Na, K. C., Seo, J. S., Kang, S. K., Kim, Y. D., 2002, Chemical compositions of Lentinula in growth stage - A study on application plan of Lentinula I -, Korean journal of plant resources, 15, 128-134.
  4. Hong, J. S., Kim, Y. H., Kim, M. K., Kim, Y. S., Sohn, H. S., 1989, Contents of Free Amino Acids and Total Amino Acids in Agaricus bisporus, Pleurotus ostreatus and Lentinus edodes, Korean journal of food science and technology, 21, 58-62.
  5. Jo, H. G., Shin, H. J., 2017, Effect of addition amino acids on the mycelial growth and the contents of ${\beta}$-glucan and ${\gamma}$-aminobutyric acid (GABA) in Sparassis latifolia, Journal of Mushrooms, 15, 38-44. https://doi.org/10.14480/JM.2017.15.1.38
  6. Kim, K. J., Seo, K. S., 2016, Free sugar, amino acid, and beta-glucan content in Lentinula edodes strains collected from different areas, Journal of mushrooms, 14, 27-33. https://doi.org/10.14480/JM.2016.14.2.27
  7. Kim, K. J., Im, S. B., Yun, K. W., Je, H. S., Ban, S. E., Jin, S. W., Jeong, S. W., Koh, Y. W., Cho, I. K., Seo, K. S., 2017, Content of proximate compositions, free sugars, amino acids, and minerals in five Lentinula edodes cultivars collected in Korea, Journal of mushrooms, 15, 216-222.
  8. Lee, J. Y., An, W. G., Lee, J. D., 1994, Studies on the Submerged Culture of Lentinula edodes Mycelia in Brewer's Yeast Extract Medium, The Korean journal of mycology, 22, 266-275.
  9. Oh, S. I., Lee, M. S., 2005, Antioxidative and antimutagenic effects of ganoderma lucidum Krast extracts, Korea Journal of food and nutrition, 18, 54-62.
  10. Pyo, M. Y., Ro, I. H., 1975, A Study on the Amino Acid Contents of Edible Mushrooms, The Korean journal of nutrition, 8, 1.
  11. Park, Y. A., Lee, K. T., Bak, W. C., Kim, M. K., Ka, K. H., Koo, C. D., 2011, Eritadenin Contents Analysis in Various Strains of Lentinula edodes using LC-MS/MS, The Korean Journal of Mycology, 39, 239-242. https://doi.org/10.4489/KJM.2010.39.3.239
  12. Park, H. S., 2014, Morphological Characteristics and Functional Components Changes of Oyster Mushroom (Pleurotus spp.) by Using Sawdust Medium Which Contains Amino acid, MS Thesis, Konkuk University.
  13. Park, Y. A., Bak, W. C., Ka, K. H., Koo, C. D., 2017, Comparative analysis of amino acid content of Lentinula edodes, a new variety of shiitake mushroom, in 'Poongnyunko', Journal of mushrooms, 15, 31-37. https://doi.org/10.14480/JM.2017.15.1.31
  14. Tian, Y., Zhao, Y., Huang, J., Zeng, H., Zheng, B., 2015, Effects of different drying methods on the product quality and volatile compounds of whole shiitake mushrooms, Journal of Food Chemistry, 197, 714-722.
  15. Yoon, D. Y., Park, K. M., Lee, J. H., 2010, Characteristics and Biological Properties of Pleurotus eryngii grown on Monosodium Glutamate-enriched Media, The Korean Society for Biotechnology and Bioengineering, 25, 277-282.