The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Analysis of the Ergothioneine Content in the Fruiting Bodies of Sawdust-cultivated Lentinula edodes Cultivars in Korea

한국의 표고 톱밥재배 품종의 에르고티오네인 함량 분석

  • Min-Jun Kim (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Yeun Sug Jeong (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Eunjin Kim (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Yeongseon Jang (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Kang-Hyeon Ka (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science)
  • 김민준 (국립산림과학원 산림생명자원연구부 산림미생물연구과) ;
  • 정연석 (국립산림과학원 산림생명자원연구부 산림미생물연구과) ;
  • 김은진 (국립산림과학원 산림생명자원연구부 산림미생물연구과) ;
  • 장영선 (국립산림과학원 산림생명자원연구부 산림미생물연구과) ;
  • 가강현 (국립산림과학원 산림생명자원연구부 산림미생물연구과)
  • Received : 2022.09.05
  • Accepted : 2022.12.19
  • Published : 2022.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Lentinula edodes is a type of mushroom widely consumed in East Asia, and many of the components contained in them are used as raw materials for pharmaceuticals or as health supplements. Ergothioneine, a sulfur-containing amino acid in L. edodes, is attracting attention due to its antioxidant and anti-inflammatory effects. L. edodes generally contain an ergothioneine content of about 200 to 800 mg per kg; there are some differences in this value depending on the cultivation conditions. In this study, 24 domestic sawdust-cultivated L. edodes cultivars were cultured under the same conditions, and the morphological characteristics and ergothioneine content of the fruiting bodies were investigated. The yield and morphological characteristics of the fruiting bodies were different in each cultivar. Taehyanggo and Sanjo 713ho had the largest and thickest pileus. The pileus of L. edodes had a higher ergothioneine content than the stipe. Sanjo 715ho had the highest ergothioneine content in the pileus and the stipe at 1,225 mg/kg and 753 mg/kg, respectively. An ergothioneine content of more than 1,000 mg/kg in the pileus was observed in Bambithyang, Sansanhyang, Sulbaekhyang, Taehyanggo, Sanjo 705ho, Sanjo 709ho, Sanjo 715ho, and Sanjo 716ho. These cultivars can be used as parental strains to develop new cultivars with enhanced ergothioneine functionalities.

본 연구에서는 국내 톱밥 재배 표고품종 24개를 같은 조건에서 배양하고 자실체를 발생시켜 형태적인 특성과 버섯의 대표적인 기능성 물질인 에르고티오네인의 함량을 조사하였다. 자실체 생산량과 형태적 특성은 품종별로 차이를 보였으며 에르고티오네인 함량이 높은 갓 부위의 경우 태향고와 산조713호가 각각 가장 크고 두껍게 측정되었다. 품종별 자실체 부위 에르고티오네인 함량 측정 결과 표고는 갓이 대보다 높은 에르고티오네인 함량을 가진다는 것을 확인할 수 있었다. 갓부위는 산조715가 kg당 1,225 mg으로 가장 높았고 대부위는 산조711가 kg당 753 mg으로 가장 높은 에르고티오네인 함량이 확인되었다. 갓부위의 에르고티오네인 함량이 kg당 1,000 mg 이상인 품종은 밤빛향, 산산향, 설백향, 태향고, 산조705호, 산조709호, 산조715호 그리고 산조716호로 이들 품종을 모균주로 선발하여 에르고티오네인의 기능성 강화 신품종 개발에 이용할 것이다.

Keywords

Acknowledgement

This study was supported by the Golden Seed Project of 'Breeding of new strains of shiitake for cultivar protection and substitution of import [213007-05-5-SBH10]' provided by the Ministry of Agriculture, Food and Rural Affairs, Ministry of Oceans and Fisheries, Rural Development Administration and Korea Forest Service and a grant from the General Project (FP0800-2020-01) of the National Institute of Forest Science, Republic of Korea.

References

  1. Aruoma O, Spencer J, Mahmood N. Protection against oxidative damage and cell death by the natural antioxidant ergothioneine. Food Chem Toxicol 1999;37:1043-53. https://doi.org/10.1016/S0278-6915(99)00098-8
  2. Shahidi F. Prevention of lipid oxidation in muscle foods by nitrite and nitrite-free compositions. Washington, D.C.: ACS Publications; 1992.
  3. Aruoma OI. Free radicals, oxidative stress, and antioxidants in human health and disease. J Am Oil Chem.' Soc 1998;75:199-212. https://doi.org/10.1007/s11746-998-0032-9
  4. Diaz M, Free B, Vita J, Keaney Jr J. Mechanisms of diseases antioxidants and atherosclerotic. Heart Disease 1997;1997:408-18.
  5. Halliwell B, Cheah IK, Tang RM. Ergothioneine- a diet-derived antioxidant with therapeutic potential. FEBS letters 2018;592:3357-66. https://doi.org/10.1002/1873-3468.13123
  6. Beelman RB, Kalaras MD, Phillips AT, Richie JP. Is ergothioneine a 'longevity vitamin' limited in the American diet? J Nutr Sci 2020;9:e52.
  7. Jang JH, Aruoma OI, Jen LS, Chung HY, Surh YJ. Ergothioneine rescues PC12 cells from β-amyloid-induced apoptotic death. Free Radic Biol Med 2004;36:288-99. https://doi.org/10.1016/j.freeradbiomed.2003.11.005
  8. Ramirez-Martinez A, Wesolek N, Yadan JC, Moutet M, Roudot AC. Intake assessment of L-ergothioneine in some European countries and in the United States. Hum Ecol Risk Assess 2016;22:667-77. https://doi.org/10.1080/10807039.2015.1104241
  9. Dubost NJ, Ou B, Beelman RB. Quantification of polyphenols and ergothioneine in cultivated mushrooms and correlation to total antioxidant capacity. Food Chem 2007;105:727-35. https://doi.org/10.1016/j.foodchem.2007.01.030
  10. Ozturk C, Atila F. Changes in lignocellulosic fractions of growing substrates during the cultivation of Hypsizygus ulmarius mushroom and its effects on mushroom productivity. Sci Hortic 2021;288:110403.
  11. Kim MJ, Jeong YS, Jang Y, Ka KH. Selection of functional Lentinula edodes strains with high ergothioneine content using mono-mono hybridization. Kor J Mycol 2021;49:507-14.
  12. Lee WH, Kim SG, Kang SJ, Kim IY, Kwon HW, Ko HK. Selection of Lentinula edodes mushroom cultivars suitable for cylindrical sawdust media. J Mushroom 2018;37:81.
  13. Cheah IK, Halliwell B. Ergothioneine; antioxidant potential, physiological function and role in disease. Biochim Biophys Acta-Mol Basis Dis 2012;1822:784-93. https://doi.org/10.1016/j.bbadis.2011.09.017
  14. Borodina I, Kenny LC, McCarthy CM, Paramasivan K, Pretorius E, Roberts TJ, van der Hoek SA, Kell DB. The biology of ergothioneine, an antioxidant nutraceutical. Nutr Res Rev 2020;33:190-217. https://doi.org/10.1017/S0954422419000301
  15. Yang X, Lin S, Lin J, Wang Y, Lin JF, Guo LQ. The biosynthetic pathway of ergothioneine in culinary-medicinal winter mushroom, Flammulina velutipes (Agaricomycetes). Int J Med Mushrooms 2020;22:171-81. https://doi.org/10.1615/IntJMedMushrooms.2020033826
  16. Yu YH, Pan HY, Guo LQ, Lin JF, Liao HL, Li HY. Successful biosynthesis of natural antioxidant ergothioneine in Saccharomyces cerevisiae required only two genes from Grifola frondosa. Microb Cell Factories 2020;19:1-10. https://doi.org/10.1186/s12934-019-1269-8
  17. Kim KJ, Im SB, Yun KW, Je HS, Ban SE, Jin SW, Jeong SW, Koh YW, Cho IK, Seo KS. Content of proximate compositions, free sugars, amino acids, and minerals in five Lentinula edodes cultivars collected in Korea. J Mushroom 2017;15:216-22.
  18. Seo S, Jang Y, Ryoo R, Ka KH. Antioxidant properties of water extracts from Lentinula edodes cultivars grown on oak log. Kor J Mycol 2018;46:51-7.
  19. Shin PG, Yoo YB, Kong WS, You CH, Oh SJ. Characterization of intraspecific hybrids by Dimon crossing in Pleurotus eryngii. J Mushroom 2004;2:109-13.
  20. Bak WC, Lee BH, Ka KH. Characteristics of new shiitake strain. Kor J Mycol 2010;38:25-8. https://doi.org/10.4489/KJM.2010.38.1.025
  21. Bak W, Park Y, Lee B, Ka K. Cultural characteristics of newly bred Lentinula edodes strain" Soohyangko". J Mushroom 2013;11:9-14. https://doi.org/10.14480/JM.2013.11.1.009
  22. Park W, Park Y, Lee B, Ka K, Park J. Characteristics of newly bred Shiitake strain Chunbaegho. Korean J Mycol 2013;41:28-32. https://doi.org/10.4489/KJM.2013.41.1.28
  23. Park Y, Bak WC, Koo CD, Lee BH. Cultural characteristics of new cultivar of Lentinula edodes, Poongnyunko. Kor J Mycol 2015;43:26-32. https://doi.org/10.4489/KJM.2015.43.1.26
  24. Ha BS, Kim S, Ro HS. Isolation and characterization of monokaryotic strains of Lentinula edodes showing higher fruiting rate and better fruiting body production. Mycobiology 2015;43:24-30. https://doi.org/10.5941/MYCO.2015.43.1.24
  25. Ka KH, Ryoo R, Jang Y, Park Y, Jeong YS, Kang JJ, Heo G, Jeon SM. Characteristics of fruiting bodies formed upon monohybrid cross of Lentinula edodes strains. Kor J Mycol 2019;47:173-9.