• Title/Summary/Keyword: cultivation conditions

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Changes of Essential Oils from Mentha piperita L. Influenced by Various Cultivation Conditions and Harvesting Time (박하의 재배조건 및 수확시기에 따른 정유성분의 변화)

  • Shin, Kyung-Eun;Park, Hong-Ku
    • Korean Journal of Food Science and Technology
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    • v.26 no.5
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    • pp.512-519
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    • 1994
  • To investigate the effects of cultivation on the aroma characteristics of essential oil of peppermint (Mentha piperita L.), experimental peppermints were cultivated with different treatments of field mulching, soil fertilization, and harvesting time. Aroma characteristics of the different mint oils were analyzed by gas chromatography (GC) and GC/mass spectrometry (MS)/infrared spectroscopy (IR). Growth of peppermint with vinyl muching was faster in the early stage of cultivation, but became almost same in the harvest date. The higher amounts of rainfall and sunshine hour resulted in the better growth of stem and leaf of the peppermint. Contents of menthone in the essential oils from the peppermint cultivated in 1990 increased as harvest date delayed from late June 25 to late July 26 the same period. In 1991 menthone decreased from June 25 to August 5, but l-menthol increased from June 25 to august 5. The results indicated that the sunshing hour affected biosynthesis of the components. Contents of menthofuran increased significantly from early June to late July. The optimum cutting date resulting best oil production was late July 7 in 1991.

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Optimized cultivation of Ettlia sp. YC001 in eutrophic pond water for nutrient removal and biomass production

  • Oh, Hyung-Seok;Ahn, Chi-Yong;Srivastava, Ankita;Oh, Hee-Mock
    • ALGAE
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    • v.33 no.4
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    • pp.319-327
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    • 2018
  • Ettlia sp. YC001, a highly settleable and productive microalga, was shown to be effective in removing nutrients and capturing suspended solids from eutrophic pond water. The optimum conditions for the Ettlia sp. YC001 cultivation were investigated using water from a landscape pond. The pond water was supplemented with different N : P ratios by weight, and the biomass production and nutrient removal compared in batch cultures. The maximum removal rate of N and P was with an N : P ratio of 16 : 1. Plus, the turbidity dropped to near zero within 4 days. Meanwhile, chemostat cultivation showed that the biomass productivity and nutrient removal rate increased when increasing the dilution rate, where a dilution rate of $0.9d^{-1}$ showed the highest N and P removal rate at $32.4mg\;L^{-1}\;d^{-1}$ and $1.83mg\;L^{-1}\;d^{-1}$, respectively, and highest biomass and lipid productivity at $0.432g\;L^{-1}\;d^{-1}$ and $67.8mg\;L^{-1}\;d^{-1}$, respectively. The turbidity was also reduced by 98% in the chemostat cultivation. Moreover, auto-flocculation and pH were closely connected to the turbidity removal. As a result, this study identified the optimal N : P ratio for small pond water treatment using an Ettlia sp. YC001, while also establishing the optimal conditions for nutrient removal, turbidity reduction, and biomass production.

Effect of Different Irrigation Levels on the Fiber Content of Sweetpotato Root

  • Won Park;Hyeong-Un Lee;Tae Hwa Kim;Kyo Hwui Lee;Sang Sik Nam;Mi Nam Chung
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.49-49
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    • 2022
  • One of the major problems with sweetpotato (Ipomoea batats Lam.) is the tough thread tissue that occurs in the storage root, which has a negative impact on the sales of sweet potato because it impairs the texture during cooking and the processing quality. The fiber contents in storage roots of sweetpotato is affected by cultivation conditions and environment. To investigate the effect of fiber generation at different levels of irrigation, the sweetpoatoto "Hogammi" was transplanted in greenhouse. Sweetpotato was grown in styrofoam beds(W1605*D330*H300mm) to block moisture flowing from the outside. The irrigation was carried out as 3 levels (5,10, and 20 mm through drip irrigation facilities) at 20-day intervals. Five plants were harvested per plot at 90, 100 and 120 days after transplanting (DAT). The size of the storage root was large in the order of irrigation conditions 10mm>20mm>5mm treatment. And the longer cultivation period, the larger size of the storage root was observed. As a result of the analysis of the fiber content, it showed a tendency to decrease as the cultivation period increased (90days→120days). In addition, the fiber contents of sweetpotato harvested at 90, 100 and 120 DAT in the level of 5 mm irrigation plot were 351, 324 and 207 mg/100g, respectively, which were higher than those of other irrigation level plots. During all cultivation periods, the 10mm treatment group showed the lowest fiber content of 280, 228 and 127 mg/100g. At 20 mm irrigation level, the fiber content was less than that of 5mm irrigation level, but showed a tendency to increase compared to that of 10 mm irrigation level. These results suggested that drought stress or excessive-irrigation increases the fiber content of sweetpotato, which reduces their commercial value.

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Production of Arachidonic Acid by Mortierella Fungi

  • Higashiyama, Kenichi;Fujikawa, Shigeaki;Park, Enoch Y.;Shimizu , Sakazu
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.5
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    • pp.252-262
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    • 2002
  • The growing interest in the application of arachidonic acid (ARA) in various fields of health and dietary requirements has elicited much attention on the industrial production of ARA-containing oil by the cultivation of Mortierella fungi. For the industrial production of ARA, various studies, such as isolation of a high-potential strain and optimization of culture conditions, have been conducted. Studies including the investigation of morphology are important because ARA is accumulated in the mycelia, and thus cultivation with high biomass concentration is essential for obtaining a high ARA yield. Combining the results derived from various studies, a high ARA yield was attained in an industrial fermentor. These ARA production techniques are applicable to the production of other polyunsaturated fatty acids (PUFAs), and will contribute to the improvement of fermentation technology especially in the field of fungal cultivation.

Cultivation of Chlorella sp. under Different Aeration Conditions Illuminated by Light Emitting Diode (LED 조명을 이용한 광생물 반응기에서 공기 주입량에 따른 클로렐라 성장 연구)

  • Choi, Bo-Ram;Lee, Tae-Yoon
    • KSBB Journal
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    • v.27 no.4
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    • pp.263-267
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    • 2012
  • The purpose of this study was to determine optimum value of aeration rate for the cultivation of Chlorella sp. under illumination of red light emitting diode. The aeration rates varied from 0 to 2.4 vvm under the illumination of 4,400 lux of red light emitting diode. The highest specific growth rate of $1.51\;day^{-1}$ was obtained at the aeration of 0.7 vvm and lower specific growth rates were obtained for other aeration tests. Furthermore, the highest biomass concentration (1.02 g/L) was also obtained at the aeration of 0.7 vvm. Therefore, aeration of 0.7 vvm was determined to be the optimum aeration rate for the cultivation of Chlorella sp. under red light emitting diode.

High-Level Production of Astaxanthin by Xanthophyllomyces dendrorhous Mutant JH1, Using Chemical and Light Induction

  • Kim Jeong-Hwan;Chang Hyo-Ihl
    • Journal of Microbiology and Biotechnology
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    • v.16 no.3
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    • pp.381-385
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    • 2006
  • The production of astaxanthin by Xanthophyllomyces dendrorhous mutant depended on the culture conditions. Therefore, a cultivation strategy, including effective chemical and light induction, for the high-level production of astaxanthin by X. dendrorhous mutant JH1 was explored. Effective chemicals such as ethanol, acetic acid, and hydrogen peroxide, which are known inducers or precursors of astaxanthin synthesis, were investigated for their increase of astaxanthin production. Each of 1.0% ethanol, 1.0% acetic acid, and 1.0% hydrogen peroxide increased the astaxanthin concentration to 49.77 mg/l, 46.33 mg/l, and 45.61 mg/l, respectively. Among these chemicals, 1.0% ethanol showed the best effect on increasing astaxanthin concentration after 48 h of cultivation. Under 1.0% ethanol feeding condition, high light intensity (2,400 lux) stimulated astaxanthin production to 59.67 mg/l, compared with that in the dark-grown cultivation.

Basic Studies on Cultivation of Transformed Plant Tissue in Bioreactor (생물반응기에서 형질전환된 식물조직 배양을 위한 기초연구)

  • 박성화;박돈희
    • KSBB Journal
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    • v.5 no.3
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    • pp.207-214
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    • 1990
  • Growth properties of carrot hairy roots transformed by Agrobacterium rhizogenes were compared in flask and bioreactor. Oxygen transfer coefficient KLa was measured during the cultivation in bioreactor. In flask cultures, initially sucrose 30g/l was nearly exhausted after 20days. pH was dropped from initially 5.8 to 4.79 after 4 days, but it is stable after that time. Finally, after 28 days, hairy roots were grown about twelve times. In view of the results studied optimum conditions, hairy roots were maintained high growth rates in sucrose 50g/l, pH 5.8, total nitrogen 60mM. Also in bioreactor cultures, fixed stainless sieve in bottom and aerated 0.31 vvm, the results of cultivation by the use of sucrose 50g/l had grown about twenty-eight times and pH variations were liked in flask. As a results, growth rate of 1.756g fresh weight/day/g inoculum in bioreactor were higher about three times than 0.57g fresh weight/day/g inoculum in flask culture. KLa values were showed a tendency to decrease from 0.209 min-1 to 0.068 min-1.

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A Study of Greenstuff Cultivation Using Enzyme Treated Nightsoil Liquid Manure (효소처리 분뇨액비를 이용한 작물재배의 실험적 연구)

  • 이수환;김종현;김복현
    • Journal of Environmental Health Sciences
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    • v.19 no.1
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    • pp.57-65
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    • 1993
  • The cultivtion efficiencies of enzyme treated nightsoil liquid manure were investigated. As a result of applying the liquid manure prepared by the nightsoil digestion due to aerobic or anoxic conditions with enzyme treatement to cultivate greenstuffs as compared with the controls (conventional cultivation) due to application of none enzyme treated fertilizer, the yield was markedly increased. In the cases of kidney bean, seedleaves were increased over 2~4 times and stem growth and diameter was increased 40% as compared the controls. Finally, cultivation yield of liquid manure prepared by the nightsoil aerobic or anoxic digestion with enzyme treatment were more increased as compared with the controls.

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