• Journal of Naturopathy
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• v.7 no.1
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• pp.20-25
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• 2018

The purpose of this study was investigated to observe the relatively low amino acid mutations in six different strains of ATCC or KCTC Salmonella strains (four species and two isolates). The mutations in the strains were to be detected and compared with the genes csgA and csgB corresponding to the Thin aggregative fimbriae. Amino acid mutations in the strains were observed from Ser20→ Gly(AGT→GGT) in the csgA gene, Asp25→Ala(GAT→GCT) and Lys66→Thr(AAA→ACA) in the csgB gene. Among the six strains, the two most common amino acid variations were observed in S. typhimurium - TH strains. On the other hand, no mutation of nucleotide sequence was observed in the strains of S. typhimurium ATCC 13311 and S. typhimurium KCTC 1925. In conclusion the genes csgA and csgB in the strains may be useful for the evaluation and detection of amino acid mutation.

• Journal of Life Science
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• v.13 no.2
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• pp.184-190
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• 2003

Myxococcus xanthus is a Gram negative, rod-shaped, soil bacterium that displays a social behaviors, and multicellular development upon nutrient deprivation. The csgA gene encoding a cell surface protein is essential for developmental behaviors including rippling, aggregation, fruiting body formation and sporulation. csgA mutants show normal vegetative growth, but lack all these developmental phenotypes. Expression of the CsgA (C-signal) specific genes are eliminated or dramatically reduced in csgA mutants. In order to identify components of C-signal transduction pathway, second site mutations were introduced into csgA mutants and were identified which can fully or partially restore development of csgA mutants (Rhie, H. G. et. al. 1989. J. Bacteriol. 171, 3268-3276). One of such csgA suppressor mutations, socD500 restores only sporulation to csgA mutants at 15$^{\circ}C$. The socD500 mutaion however eliminates the three basic developmental requirements, starvation, high cell density and a solid surface. Only sporulation, not accompanied with fruiting body formation is induced simply by shifting the temperature of vegetatively growing cells from $32^{\circ}C$ to $15^{\circ}C$. Spores induced by socD500 mutation is not as thick as that of wild-type fruiting body. In socD500 genetic background, two of ten C-signal dependent genes, $\Omega$DK4506 and $\Omega$DK4406 are more highly expressed in growing cells at $15^{\circ}C$. These results indicate that the socD500 mutation may be partly involved in the regulation of expression of two C-signal dependent genes and genes for sporulation in this transduction pathway.

• Journal of The Korean Society of Agricultural Engineers
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• v.52 no.1
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• pp.51-59
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• 2010

The cemented sand and gravel (CSG) method is a construction technique that adds cement and water to rock-like materials, such as rivered gravel or excavation muck which that can be obtained easily at areas adjacent to dam sites. This study was performed to evaluate the unconfined compressive strength properties and freezing and thawing resistance of CSG materials with unit cement content. The three types of CSG-80, CSG-100 and CSG-120 with cement content were designed to evaluate the optimum water content, dry density, strength, stress-strain, micro structure and durability factor. As the results, the optimum water content ratio with cement content showed almost similar tendency, and the unconfined compressive strength and dry density increased as cement content increases. The strength ratio of 7 days for 28 days were in the range of 55~61 % and the strain ratio in stress-strain curve were in the range of 0.8~1.6 % nearby maximum strength in 28 days. It is expected that this study will contribute to increasing application of CSG method as well as to increasing the utilizing of CSG materials as a environmentally friendly CSG method.

• Journal of the Korea Computer Graphics Society
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• v.3 no.2
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• pp.77-84
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• 1997

Solid modeling refers to techniques for unambiguous representations of three- dimensional objects. The most widely used techniques for solid modeling have been Constructive Solid Geometry (CSG) and Boundary Representation (BRep). Contemporary solid modeling systems typically support both representations, and bilateral conversions between CSG and BRep are essential. However, computing a CSG from a BRep is largely an open problem. This paper presents 3D geometric reasoning algorithms for converting a BRep into a special CSG, called Destructive Solid Geometry (DSG) whose Boolean operations are all subtractions. The major application area of BRep-to-DSG conversion is feature recognition, which is essential for integrating CAD and CAM.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.8
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• pp.2590-2606
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• 2014

A problem related to deployment of femtocells is how to manage access of users to radio resources. On one hand, all resources of the femtocell can be reserved for users belonging to a closed subscriber group (CSG), which is a set of users defined by a femtocell subscriber. This approach, known as closed access, however, increases interference to users not included in the CSG as those users do not have a permission to access this femtocell. Contrary, resources can be shared by all users with no priority in an open access mode. In this case, the femtocell subscriber shares radio as well as backhaul resources with all other users. Thus, throughput and quality of service of the subscriber and the CSG users can be deteriorated. To satisfy both the CSG as well as non-CSG users, a hybrid access is seen as a compromise. In this paper, we propose a new approach for sharing radio resources among all users. As in common cases, the CSG users have a priority for usage of a part of resources while rest of the resources is shared by all users proportionally to their requirements. As the simulation results show, the proposed resource sharing scheme significantly improves throughput of the CSG users and their satisfaction with granted bitrates. At the same time, throughput and satisfaction of the non-CSG users is still guaranteed roughly at the same level as if conventional sharing schemes are applied.

• Korean Journal of Microbiology
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• v.47 no.4
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• pp.335-341
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• 2011

In this study, 22 clinical isolates of Enterobacteriaceae including Citrobacterfreundii (6 strains), Enterobacter cloacae (5 strains), Enterobacter aerogenes (3 strains), Serratia marcescens (7 strains) and Pantoea spp. (1 strain) were investigated for the biofilm forming ability and biosynthesis of curli and cellulose. Biofilm forming ability was the highest among the isolates of E. cloacae and the lowest among the isolates of E. aerogenes. The expression of the biofilm-forming extracellular matrix components, cellulose and curli fimbriae, was examined by Congo-red (CR) staining and calcofluor staining methods. PCR screening for the presence of curli gene (csgA) revealed that 4 strains of E. cloacae and 1 strain of C. freundii carried the csgA, showing a good correlation between the phenotypic detection of curli fimbriae by CR staining method and the genotypic detection of curli gene by PCR in E. cloacae.

• Proceedings of the Korean Geotechical Society Conference
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• 2006.03a
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• pp.61-68
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• 2006

Cemented Sand and Gravel(CSG) is a material made by simple mixing of rock-based raw materials such as excavated soil and riverbed gravel together with cement and water. The use of CSG methodl for cofferdam and large dam is gradually increasing in Japan because a quarry and aggregate plants can be diminished. Also, the CSG method can reduce dam construction cost, construction duration and destruction of environment. In this paper, field test and test construction of CSG method was conducted on Hwabuk Dam. The mechanical properties of CSG, such as compressive strength, extention strength and field permeability test were investigated. From the results of the experimental study, application study of CSG method was discussed.

• Journal of Digital Contents Society
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• v.9 no.2
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• pp.363-369
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• 2008

In this paper, we have proposed 3D shape reconstruction using superquadrics and single z-buffer Constructive Solid Geometry (CSG) rendering algorithm. Superquadrics can obtain various 3D model using 11 parameters and both superquadrics and deformed-superquadrics play a role of primitives which are consisted of CSG tree. In addition, we defined some effective equations using z-buffer algorithm and stencil buffer for synthesizing 3D model. Using this proposed algorithm, we need not to consider the coordinate of each 3D model because we simply compare the depth value of 3D model.

• Journal of The Korean Society of Agricultural Engineers
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• v.52 no.4
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• pp.73-81
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• 2010

The cemented sand and gravel (CSG) method is a construction technique that adds cement and water to rock-like materials, such as rivered gravel or excavation muck which can be obtained easily at areas adjacent to dam sites. This study was performed to evaluate the compaction and compressive strength properties of stress-strain, elastic modulus and fracture mode CSG materials reinforced polypropylene fiber. Polypropylene fiber widely used for concrete reinforcement is randomly distributed into cemented sand. The two types of polypropylene fiber (monofillament and fibrillated fiber) were used and fiber fraction ratio was 0, 0.2 %, 0.4 %, 0.6 % and 0.8 % by the weight of total dry soil. The effect of fiber fraction ratio and fiber shape on compaction and compressive strength were investigated. The optimum moisture contents (OMC) of CSG material increased as fiber fraction increased and the dry density of CSG material decreased as fiber fraction. Also, the maximum increase in compressive strength was obtained at 0.4 % content of monofillament and fibrillated fiber. CSG material behaviour was controlled not only by fiber fraction but also fiber distribution, fiber shape and fiber type.

• Journal of the Korean Institute of Telematics and Electronics
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• v.27 no.2
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• pp.151-157
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• 1990

This paper presents a method for rapid wire frame drawing of the 3D objects represented by the CSG scheme. When the two CSG trees are combined into one, the intersection parts of the polygons constituting the object corresponds to each subtree are computed, and the boundary representation of the combined object is obtained according to the given combinational operator and stored in the root node. The boundary representation in the root node is used in the wireframe drawing of the object and later computation of boundary representation. Bezier surface is taken as one of the primitive object the scan-line algorithm is used, which subdivides each scan-line into the spans where no polygon is intersected, and renders each span with the CSG representation of the object.