• Journal of Preventive Veterinary Medicine
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• 제43권4호
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• pp.214-220
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• 2019

Although canine brucellosis has been known to be an important re-emerging zoonosis, the pathophysiological mechanisms of Brucella canis infection remains clues to be solved. Different culture models, single and co-culture models, were constructed with canine epithelial cells, D17 and macrophage, DH82 to investigate the induction of immune responses in in vivo B. canis infection. Expression of genes related with induction of immune responses, Th1, Th2 and Th17, was compared in the two different models after the bacterial infection. In this study, expression of cytokine genes, IL-1β, IL-5, IL-6, IL-10, IL-23, and TNF-α was quantified in the DH82 at different time points using RT-qPCR in the two different culture systems after the infection. Cytokine genes related with Th1, IL-1β and TNF-α and Th17, IL-6 and IL-23 were expressed with time-dependent manners in the both systems (p<0.05). However, increase of Th2-related cytokine genes expression was not detectable in the both systems by comparison with control. The expression of Th1 and Th17 related cytokine genes was earlier in single cell culture than those in co-culture model (p<0.05). In general, amounts of the expressed genes were shown higher in single cell model than those in co-culture models. This study indicate that Th1 and Th17-associated immune responses are central to B. canis infection in dogs. In addition, it suggests a specific role of epithelial cells in the B. canis infection in vivo, which should resolved in the further study.

• The Korean Journal of Physiology and Pharmacology
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• 제1권3호
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• pp.285-296
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• 1997

Injury to brain transforms resting astrocytes to their reactive form, the hallmark of which is an increase in glial fibrillary acidic protein (GFAP), the major intermediate filament protein of their cell type. The overall glial response after brain injury is referred to as reactive gliosis. Glial-neuronal interaction is important for neuronal migration, neurite outgrowth and axonal guidance during ontogenic development. Although much attention has been given to glial regulation of neuronal development and regeneration, evidences also suggest a neuronal influence on glial cell differentiation, maturation and function. The aim of the present study was to analyze the effects of glial-hippocampal neuronal co-culture on GFAP expression in the co-cultured astrocytes. The following antibodies were used for double immunostaining chemistry; mouse monoclonal antibodies for confirm neuronal cells, rabbit anti GFAP antibodies for confirm astrocytes. Primary cultured astrocytes showed the typical flat polygonal morphology in culture and expressed strong GFAP and vimentin. Co-cultured hippocampal neurons on astrocytes had phase bright cell body and well branched neurites. About half of co-cultured astrocytes expressed negative or weak GFAP and vimentin. After 2 hour glutamate (0.5 mM) exposure of glial-neuronal co-culture, neuronal cells lost their neurites and most of astrocytes expressed strong CFAE and vimentin. In Western blot analysis, total GFAP and vimentin contents in co-cultured astrocytes were lower than those of primary cultured astrocytes. After glutamate exposure of glial-neuronal co-culture, GFAP and vimentin contents in astrocytes were increased to the level of primary cultured astrocytes. These results suggest that neuronal cell decrease GFAP expression in co-cultured astrocytes and hippocampal neuronal-glial co-culture can be used as a reactive gliosis model in vitro for studying GFAP expression of astrocytes.

• The Korean Journal of Pain
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• 제35권2호
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• pp.160-172
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• 2022

Background: The authors established an in vitro model of diabetic neuropathy based on the culture system of primary neurons and Schwann cells (SCs) to mimic similar symptoms observed in in vivo models of this complication, such as impaired neurite extension and impaired myelination. The model was then utilized to investigate the effects of insulin on enhancing neurite extension and myelination of diabetic neurons. Methods: SCs and primary neurons were cultured under conditions mimicking hyperglycemia prepared by adding glucose to the basal culture medium. In a single culture, the proliferation and maturation of SCs and the neurite extension of neurons were evaluated. In a co-culture, the percentage of myelination of diabetic neurons was investigated. Insulin at different concentrations was supplemented to culture media to examine its effects on neurite extension and myelination. Results: The cells showed similar symptoms observed in in vivo models of this complication. In a single culture, hyperglycemia attenuated the proliferation and maturation of SCs, induced apoptosis, and impaired neurite extension of both sensory and motor neurons. In a co-culture of SCs and neurons, the percentage of myelinated neurites in the hyperglycemia-treated group was significantly lower than that in the control group. This impaired neurite extension and myelination was reversed by the introduction of insulin to the hyperglycemic culture media. Conclusions: Insulin may be a potential candidate for improving diabetic neuropathy. Insulin can function as a neurotrophic factor to support both neurons and SCs. Further research is needed to discover the potential of insulin in improving diabetic neuropathy.

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
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• pp.132.1-132.1
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• 2001

• 한국식생활문화학회지
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• 제3권1호
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• pp.23-27
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• 1988

일본, 한국, 중국의 식문화에 관한 용어들을 조사 비교하였다. 우선 맛의 인지를 표현하는 용어들을 수집 조사하고 이어서 삼개국 용어중에 서로 관계가 있는 단어들을 모으고 정의하였으며 맛 인식표현에서 삼개국 용어모델을 수립하였다.

• 한국식품저장유통학회지
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• 제16권2호
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• pp.253-258
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• 2009

This study investigated the effects of mycelium and culture supernatant of Cordyceps ochraceostromat(Co) on air way hyper-responsiveness, pulmonary immune cell infiltration, and Th2 cytokine expression in animal models of atopy and asthma. After ConA(+/-) activation of mouse primary spleen cells, decreased IL-4 and IL-13 cytokine production were seen in the presence of Co mycelium extracts and culture supernatant. The asthma model involved mice sensitized to ovalbumin by i.p. injection treatment; Co mycelium extract was also injected. The atopy model was the dinitrofenylbenzene-treated mouse ear. Ear thicken ing induced by DNFB was decreased by Co mycelium extract, and the extract also inhibited lung cell infiltration in ovalbumin-induced asthmatic mice. The results thus indicated that the Co mycelial extract reduced the undesirable immune responses seen in asthma and atopy.

• 한국콘텐츠학회논문지
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• 제15권12호
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• pp.407-420
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• 2015

본 연구는 연구개발 직군의 실행공동체 영향요인이 공동체 활동 만족도와 조직 내 학습문화 활성화에 미치는 관계를 분석하고, 실행공동체의 성숙도에 따른 성숙 집단과 미성숙 집단의 비교를 통해 기업의 실행공동체 담당자들에게 연구개발 직군의 실행공동체 활성화를 위한 시사점을 제시하고자 했다. 이를 위해 선행연구를 토대로 실행공동체 영향요인을 개인요인, 상호작용요인, 지원요인, 환경요인으로 구성하고, 수집된 총 371개의 설문을 기반으로 분석을 실시했다. 연구결과 연구 개발 직군의 실행공동체 활동에 있어 개인요인, 상호작용요인, 지원요인은 만족도에 유의미한 영향을 미쳤으나 환경요인은 크게 영향을 미치지 않는 것으로 나타났다. 또한 실행공동체 만족도가 높을수록 학습문화 활성화에 긍정적 영향을 미치는 것으로 나타나 연구개발 직군의 실행공동체 활동 강화가 조직문화 개선에 도움을 줄 수 있음을 알 수 있었다. 특히 연구개발 직군의 경우 성숙도에 따른 집단차이가 크게 나타나지 않아 성숙도 보다는 실행공동체 내 상호작용 요인을 강화하는 것이 더욱 중요하다는 사실을 알 수 있다.

• 유통과학연구
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• 제11권12호
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• pp.13-23
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• 2013

Purpose - There is a dominant opinion that medium and small enterprises in the Korean economy have not developed qualitatively but only towards quantitative growth and, therefore, the unbalanced structure between large enterprises and those that are medium and small has worsened. In particular, this rapid industrialization causes after-effects such as polarization as well as anti-business sentiment, the collapse of the middle class, and hostility against the establishment. The consensus contends that it is difficult for Korea to be an advanced nation without resolving these problems. This paper attempts to suggest a co-prosperity model by limiting the focus to business relations with medium and small manufacturers (with regard to investment among the various co-prosperity institutions of POSCO). These co-prosperity institutions have been established in POSCO; however, it is thought that the development of a co-prosperity model regarding investment in medium and small manufacturers will help many needy investment manufacturers. Research design, data, and methodology - This study analyzes research on the co-prosperity model, using it to examine Korean cases and foreign cases. The co-prosperity model has been continuously extended but is determined to be seriously insufficient. The purpose of this study is to develop the Korean co-prosperity model by reinterpreting it in various aspects. In order to develop the Korean co-prosperity model, this study suggests the case of the establishment of the co-prosperity model by POSCO with medium and small manufacturers with regard to investment. This model is expected to be presented to many enterprises as the future co-prosperity model. Results - To date, analysis of the co-prosperity model itself and the co-prosperity model through the case of POSCO have been suggested. As empirical studies on co-prosperity in Korea are not sufficient, successful models of co-prosperity should be developed in various aspects in future. It is expected that through this study, medium and small manufacturers would have an opportunity to find various growth engines by actively using the cooperation platform and establishing optimized competitiveness of steel material through a steel business model. The ecosystem of enterprises may evolve and be healthier by making more joint products through productive business relationships between large enterprises and those that are medium and small. From the enterprises' ecosystem viewpoint, cooperation between such businesses rather than one-way support is identified as an essential element for the security of inter-competitiveness. Conclusions - Infrastructure should be established to form a dynamic industry ecosystem not by transient efforts in co-prosperity, but by an entire culture of co-prosperity across industries. In this respect, the leading role of public institutions needs to be intensified initially. In addition, the effects of co-prosperity should be extended to blind spots of policies such as third party companies and regions. A precise co-prosperity monitoring system should be established to continuously conduct and extend these efforts.

• Nutrition Research and Practice
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• 제9권1호
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• pp.3-10
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• 2015

BACKGROUND/OBJECTIVES: Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, involves chronic inflammation of the gastrointestinal tract. Previously, Sasa quelpaertensis leaves have been shown to mediate anti-inflammation and anti-cancer effects, although it remains unclear whether Sasa leaves are able to attenuate inflammation-related intestinal diseases. Therefore, the aim of this study was to investigate the anti-inflammatory effects of Sasa quelpaertensis leaf extract (SQE) using an in vitro co-culture model of the intestinal epithelial environment. MATERIALS/METHODS: An in vitro co-culture system was established that consisted of intestinal epithelial Caco-2 cells and RAW 264.7 macrophages. Treatment with lipopolysaccharide (LPS) was used to induce inflammation. RESULTS: Treatment with SQE significantly suppressed the secretion of LPS-induced nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), IL-6, and IL-$1{\beta}$ in co-cultured RAW 264.7 macrophages. In addition, expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and tumor necrosis factor (TNF)-${\alpha}$ were down-regulated in response to inhibition of $I{\kappa}B{\alpha}$ phosphorylation by SQE. Compared with two bioactive compounds that have previously been identified in SQE, tricin and P-coumaric acid, SQE exhibited the most effective anti-inflammatory properties. CONCLUSIONS: SQE exhibited intestinal anti-inflammatory activity by inhibiting various inflammatory mediators mediated through nuclear transcription factor kappa-B (NF-kB) activation. Thus, SQE has the potential to ameliorate inflammation-related diseases, including IBD, by limiting excessive production of pro-inflammatory mediators.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권4호
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• pp.274-277
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• 2004

Production of green fluorescent protein (GFP) as a model foreign protein using different culture scales under co-expression of Vitreoscilla hemoglobin (VHb) in the industrial Escherichia coli strain W3110 (a K12 derivative), was examined. It was found that the VHb co-expressing W3110, exhibited an exceptional and sustained production ability during cell cultures using different scales, while the VHb non-expressing strain showed variable production levels. This high and sustained production ability indicates that the VHb co-expressing E. coli W3110, could be successfully employed for practical large-scale production cultures without the need for serious consideration of scale-up problems.