Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Comparative Production of Green Fluorescent Protein Under Co-expression of Bacterial Hemoglobin in Escherichia coli W3110 Using Different Culture Scales

  • Bassapa Johnvesly (Department of Chemical Engineering, Pohang University of Science and Technology) ;
  • Kang, Dong-Gyun (Department of Chemical Engineering, Pohang University of Science and Technology, Division of Molecular and Life Sciences, Pohang University of Science and Technolog) ;
  • Park, Suk-Soon (Department of Environmental Engineering, Semyung University) ;
  • Kim, Ji-Hyun (Department of Chemical Engineering, Dongguk University) ;
  • Cha, Hyung-Joon (Department of Chemical Engineering, Pohang University of Science and Technology, Division of Molecular and Life Sciences, Pohang University of Science and Technology)
  • Published : 2004.07.01
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Abstract

Production of green fluorescent protein (GFP) as a model foreign protein using different culture scales under co-expression of Vitreoscilla hemoglobin (VHb) in the industrial Escherichia coli strain W3110 (a K12 derivative), was examined. It was found that the VHb co-expressing W3110, exhibited an exceptional and sustained production ability during cell cultures using different scales, while the VHb non-expressing strain showed variable production levels. This high and sustained production ability indicates that the VHb co-expressing E. coli W3110, could be successfully employed for practical large-scale production cultures without the need for serious consideration of scale-up problems.

Keywords

References

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