• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.39-39
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• 2003

In our previous study, WEHI-231, an immature B cell line, showed intractable increase in [C $a^{2+}$]$_{c}$ after the B-cell receptor (BCR) ligation and treatment with 2-aminoethoxydiphenylborate (2-APB), which was never observed in Bal-17, a mature B cell line (Nam et al., 2003, FEBS Lett). In this study, a whole cell voltage clamp study revealed a specific expression of a novel type of $K^{+}$ current, namely voltage-independent background-type $K^{+}$ channels (IK-bg), in WEHI-231 cells. IK-bg was dramatically increase by the application of 2-APB (50 $\square$M), which induced severe hyperpolarization of WEHI-231 from -45 ㎷ to -90 ㎷, When dialyzed with $Mg^{2+}$ and ATP-free pipette solution, a spontaneous development of IK-bg and membrane hyperpolarization were observed. IK-bg was insensitive to classical $K^{+}$ channel blockers (TEA, glibenclamide, $Ba^{2+}$(1 mM)), whereas blocked by quinine and quinidine in a voltage-dependent manner ($IC_{50}$/=6~9 $\square$M at +60㎷). Phorbol myrstate, a PKC activator, decreased the amplitude of IK-bg. Extracellular acidification (pH 6.5) slightly inhibited IK-bg. Arachidonic acid, riluzole, or hyposmotic stress could not affect the IK-bg after the full development by the intracellular dialysis with Mg-ATP-free solution. In a cell-attached mode of single channel recording from WEHI231, we found two types of voltage-independent $K^{+}$ channels with unitary conductance of 300 pS and 120 pS, respectively. Both channels showed very short mean open times and their open probabilities were increase by the application of 2-APB. In Bal-17 cells, no such $K^{+}$ current was observed in 50 cells tested. In summary, WEHI-231 immature B cells express background $K^{+}$ channels. The pharmacological properties and the large unitary conductance suggest that novel types of two-pore domain $K^{+}$ channels (2-P-K channels) might be expressed in WEHI-231, which may provide an intriguing targets of signal transduction in the immature B lymphocytes.e B lymphocytes.

• Journal of the Korea Computer Graphics Society
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• v.26 no.3
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• pp.9-19
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• 2020

With the recent development of various kinds of virtual reality devices, there has been an active research effort to increase the sense of reality by recognizing the physical behavior of users rather than by classical user input methods. Among such devices, the Leap Motion controller recognizes the user's hand gestures and can realistically trace the user's hand in a virtual reality environment. However, manipulating an object in virtual reality using a recognized user's hand often causes the hand to pass through the object, which should not occur in the real world. This study presents a way to build a visual feedback system for enhancing the user's sense of interaction between hands and objects in virtual reality. In virtual reality, the user's hands are examined precisely by using a ray tracing method to see if the virtual object collides with the user's hand, and when any collision occurs, visual feedback is given through the process of reconstructing the user's hand by moving the position of the end of the user's fingers that enter the object through sign distance field and reverse mechanics. This enables realistic interaction in virtual reality in real time.

• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.2
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• pp.99-104
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• 1983

Chemical changes of major tea constituents by tea manufacture were examined by the quantitative masurements of tannin, caffeine, free sugars and total amino acid. The sample used in this experiments was fresh green and black tea leaves, green tea prepared by classical panning process and black tea prepared by commercial process. The results obtained are as follows: Compared with fresh tea leaves, tannin was increased 2% in green tea and decreased 56.5% in black tea by tea manufacture. Caffeine was increased 25.9% in green tea and decreased 3.1% in black tea. Total free sugars were decreased 78.2% and 76.7% in green tea and black tea, respectively. Total amino acids were decreased 26.1% and 18.8% in green tea and black tea, respectively.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.29 no.6
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• pp.529-538
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• 2016

The simplified plate theory is presented for static and free vibration analysis of power-law(P) and sigmoid(S) Functionally Graded Materials(FGM) plates. This theory considers the parabolic distribution of the transverse shear stress, and satisfies the condition that requires the transverse shear stress to be zero on the upper and lower surfaces of the plate, without the shear correction factor. The simplified plate theory uses only four unknown variables and shares strong similarities with classical plate theory(CPT) in many aspects such as stress-resultant expressions, equation of motion and boundary conditions. The material properties of the plate are assumed to vary according to the power-law and sigmoid distributions of the volume fractions of the constituents. The Hamilton's principle is used to derive the equations of motion and Winkler-Pasternak elastic foundation model is employed. The results of static and dynamic responses for a simply supported FGM plate are calculated and a comparative analysis is carried out. The results of the comparative analysis with the solutions of references show relevant and accurate results for static and free vibration problems of FGM plates. Analytical solutions for the static and free vibration problems are presented so as to reveal the effects of the power law index, elastic foundation parameter, and side-to-thickness ratio.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.30 no.4
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• pp.335-341
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• 2017

In this paper, beam finite elements with higher-order derivatives' continuity are formulated and evaluated for various boundary conditions. All the beam elements are based on Euler-Bernoulli beam theory. These higher-order beam elements are often required to analyze structures by using newly developed higher-order beam theories and/or non-classical beam theories based on nonlocal elasticity. It is however rare to assess the performance of such elements in terms of boundary and loading conditions. To this end, two higher-order beam elements are formulated, in which $C^2$ and $C^3$ continuities of the deflection are enforced, respectively. Three different boundary conditions are then applied to solve beam structures, such as cantilever, simply-support and clamped-hinge conditions. In addition to conventional Euler-Bernoulli beam boundary conditions, the effect of higher-order boundary conditions is investigated. Depending on the boundary conditions, the oscillatory behavior of deflections is observed. Especially the geometric boundary conditions are problematic, which trigger unstable solutions when higher-order deflections are prescribed. It is expected that the results obtained herein serve as a guideline for higher-order derivatives' continuous finite elements.

• Journal of Korean Tunnelling and Underground Space Association
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• v.19 no.2
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• pp.213-229
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• 2017

Recently, in urban areas there is a tendency to construct more complex network-type tunnels including entrance and exit ramps. At the same time, various one-dimensional programs based on the network theory have been proposed for tunnel ventilation analysis. This paper aims at developing a program that can analyze the ventilation flow rate and pollutants concentration in complex network-type tunnels based on the none hardy-cross method. The flow analysis in the branch was carried out on the basis of the Gradient method, while for the concentration analysis a new logic has been developed to calculate the inflow and outflow concentration automatically in a complex network-type structure. Additionally, in the tunnel segments showing low flow rate, proper grid interval sizes were proposed to reduce numerical error. To verify the applicability of the program, flow rates predicted in the straight tunnels were compared with the classical velocity-diagram method by Stokic and the TVSDM program. The results showed that the errors were within 1%. In addition, the program was applied to the recent ventilation system adopted in the complex network-type urban tunnels.

• Proceedings of the Korea Society for Simulation Conference
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• 1997.04a
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• pp.114-114
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• 1997

항만개발 사업은 매립이나 준설과 같은 해안선과 해저지형을 변화시키는 공사가 이루어지며 이는 조석조류 현상의 변화를 유발하게 된다. 특히 해안지형이 복잡한 항만이나 하구에서는 변화정도가 크게 나타나며 방파제나 선박접안 시설과 같은 항내 구조물을 축조 할 경우에도 조석조류 변화가 야기된다. 조석조류 현상의 변화는 해양 수질과 생물상에 영 향을 줄뿐만 아니라 인접한 육지에 이르기까지 크다란 피해를 유발할 수가 있다. 창조시 해 수를 육지로 범람하게 하거나 퇴적이나 침식조건을 변화시킬 수도 있으며 오염물질이 외해 로 확산되는 과정을 방해하고 해양생물의 서식조건을 악화시켜 부근 해역의 어업에 피해를 유발할 수도 있다. 또한 해안 공사 과정에 해수로 유입되는 토사나 항만 이용시에 배출되는 폐수 등이 항내 수질과 생태계를 변화시킬 수 있다. 따라서 조석조류와 수질변화를 사전에 예측하고 이를 최소화하는 방안이 강구되어야 한다. 조석조류와 수질 변화를 사전에 예측하 는 방법으로 축소모형 실험과 컴퓨터 시뮬레이션이 사용된다. 축소모형 실험은 현장을 재현 하는데 많은 경비와 시간이 소모되며 예측에 한계가 있다. 반면에 컴퓨터 시뮬레이션은 대 상해역의 지형과 조류 그리고 수질특성에 적합하게 모델을 개발할 경우 예측력이 매우 높기 때문에 현재 가장 널리 이용되고 있다. 본 연구는 유한 요소법에 기초하여 미국 공병단 (United States Army Corps of Engineers)에서 개발한 TABS-2 모델을 이용하여 온산항에 서 시행되는 부두건설에 따른 조석조류 및 수질변화를 시뮬레이션하였다. 측정된 자료로 모 델 계수를 보정하고 검증하였으며 항내 매립과 준설이 이루어질 때 나타나는 조위와 조속변 화를 예측하였다. 매립과 준설공사시 유출되는 토사가 조석조류현상에 따라 이동 확산되는 범위와 농도분포를 예측하였다.ophobic components)은 75~80%, 친수성 물질(hydrophilic components)은 30~33%정도의 분포를 보였고, 전염소 및 전오존 공정을 거친 처리수에서는 각각 62.2-62.8%, 43.9~49.0% 및 50~ 55%, 40~57% 정도의 분포를 보였다. 그리고 웅집-침전을 거친 처리수에서는 그 분포가 77~82%, 24-48%였다. 전주리 공정을 통하여 소수성 물질(byoghobic components)의 분포가 감 소하는 것을 볼 때 전염소 및 전오존 처리가 용존유기물의 응집에는 오히려 역 효과를 나타내는 것으로 판단된다. 것으로 판단된다.여 황토 2g에 대하여 Hieltijes and Lijklema 방법에 의해 Adsorbed-p, Nonapatite inorganic-P(NAI-P), Apatite-p, Organic-P로 구분하여 분석하고, 총인(Total Phosphorus)을 Standard Methods에 따라 Persulfate digestion후 0.45 m membrane 여지 여과하여 여액에 대해 PO3-4-P의 농도를 Ascorbic Acid 법으로 측정한 결과, NAI-P가 가장 큰 비율을 차지하였고, 부원료로 첨가된 금속 양이온 중 Fe3-이온이 흡착에 기여하는 정도가 가장 큰 것으로 평가되었다.다당류 T-AS의 보체 활성화 기작은 classical과 alternative complement pathway의 양 경로를 통해 활성화 되었다. T-AS 분획은 mouse내의 특정 혈청단백을 증가시켰으며, 항체 생성능의 증가가 관찰되어 effect T 세포의 활성화가 나타나고 있음을 알 수 있었다. T-AS는 생체내 투여시에 대식세포의 탐식능이 증진되었으며, 대식세포 기능 저해제에 의한 대식세포의 기능 저해 현상이 회복되었다. 이와 같은 결과들로부터, T-AS의 항암 활성은 활성

• Korean Journal of Plant Taxonomy
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• v.40 no.1
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• pp.1-15
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• 2010

Recognizing a basal group in a taxon is one of the most important factors involved in understanding the evolutionary history of that group of life. Many botanists have suggested a sister to all other angiosperms to understand the origin and rapid diversification of angiosperms based on morphological and fossil evidence. Recent technical advances in molecular biology and the accumulation of molecular phylogenetic data have provided evidence of the extant basal-most angiosperm which is a sister to all other angiosperms. Although it is still arguable, most plant taxonomists agree that Amborella trichopoda Baill., a species (monotypic genus and monotypic family) distributed in New Caledonia, is a sister to all other extant angiosperms based on evidence from the following molecular approaches: 1) classical phylogenetic analyses based on multiple genes (or DNA regions), 2) analyses of a tree network of duplicated gene families, and 3) gene-structural evidence. As an alternative hypothesis with relatively minor evidence, some researchers have also suggested that Amborella and Nymphaeaceae form a clade that is a sister to all other angiosperms. Debate regarding the basal-most angiosperms is still ongoing and is currently one of the hot issues in plant evolutionary biology. We expect that sequencing of the whole genome of Amborella as an evolutionary model plant and subsequent studies based on this genome sequence will provide information regarding the origin and rapid diversification of angiosperms, which is Darwin's so called abominable mystery.

• Journal of the Society of Naval Architects of Korea
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• v.55 no.5
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• pp.401-414
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• 2018

Planing hull type ships are often equipped with interceptor or trim tab to improve the excessive trim angle which leads to poor resistance and sea keeping performances. The purpose of this study is to design a controller to control the attitude of the ship by controllable stern interceptor and validate the effectiveness of the attitude control by the towing tank test. Embedded controller, servo motor and controllable stern interceptor system were equipped with planing hull type model ship. Prior to designing the control algorithm, a model test was performed to identify the system dynamic model of the planing hull type ship including the stern interceptor. The matrix components of model were optimized by Genetic Algorithm. Using the identified model, PID controller which is a classical controller and sliding mode controller which is a nonlinear robust controller were designed. Gain tuning of the controllers and running simulation was conducted before the towing tank test. Inserting the designed control algorithm into the embedded controller of the model ship, the effectiveness of the active control of the stern interceptor was validated by towing tank test. In still water test with small disturbance, the sliding mode controller showed better performance of canceling the disturbance and the steady-state control performance than the PID controller.

• Journal of the Korea Society of Computer and Information
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• v.16 no.7
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• pp.105-115
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• 2011

In Vision-based Intelligent Transportation System(ITS) the segmentation of road region is a very basic functionality. Accordingly, in this paper, we propose a region segmentation method using adaptive pattern extraction technique to segment road regions and sky regions from original images. The proposed method consists of three steps; firstly we perform the initial segmentation using Mean Shift algorithm, the second step is the candidate region selection based on a static-pattern matching technique and the third is the region growing step based on a dynamic-pattern matching technique. The proposed method is able to get more reliable results than the classic region segmentation methods which are based on existing split and merge strategy. The reason for the better results is because we use adaptive patterns extracted from neighboring regions of the current segmented regions to measure the region homogeneity. To evaluate advantages of the proposed method, we compared our method with the classical pattern matching method using static-patterns. In the experiments, the proposed method was proved that the better performance of 8.12% was achieved when we used adaptive patterns instead of static-patterns. We expect that the proposed method can segment road and sky areas in the various road condition in stable, and take an important role in the vision-based ITS applications.