• Journal of the Korean Society of Manufacturing Process Engineers
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• v.13 no.2
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• pp.1-7
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• 2014

Recent experimental observations support the hypothesis that mechanical stimuli play a role in regulating the specialized molecular expression of articular cartilage in vitro and in vivo. Other studies have demonstrated that the continuous passive motion(CPM)bioreactor for whole joints can provide a platform for possible future in vitro studies and applications, including possible interactions of bio-mechanical and biochemical signals. In this study, we have developed acustom-made bioreactor capable of bending and stretching with circular type motion, and a biomimetic knee joint model, using a 3D printer. This system could be used to investigate the effects of rehabilitative joint motion of dynamic culture.

• Journal of Institute of Control, Robotics and Systems
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• v.8 no.3
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• pp.208-219
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• 2002

A novel measurement method to obtain the 6-DOF motions of arbitrary rigid bodies is proposed in this paper. The method adopts a specially fabricated mirror called 3-facet mirror, which looks like a triangular pyramid haying an equilateral cross-sectional shape. The mirror is mounted on the objects to be measured, illuminated by a laser beam having circular profile, and reflects the laser beam in three different directions. Three PSDs(position sensitive detector) detect the three beams reflected by the mirror, respectively. From the signals of the PSDs, we can calculate the 3-dimensional position and orientation of the 3-facet mirror, and thus enabling us to determine the 3-dimensional position and orientation of the objects. In this paper, we model the relationship between the 3-dimensional position and orientation of an object in motion and the outputs of three PSDs. A series of experiments are performed to demonstrate the effectiveness and accuracy of the proposed method. The experimental results show that the proposed sensing system can be an effective means of obtaining 3-dimensional position and orientation of arbitrary objects and provide resonable measurement accuracy.

• Journal of electromagnetic engineering and science
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• v.7 no.4
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• pp.161-168
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• 2007

A fast correlative vector direction finding(CVDF) system using active dipole antenna array for mobile direction finding(DF) applications is presented. To develop the CVDF system, the main elements such as active dipole antenna, multi-channel direction finder, and search receiver are designed and analyzed. The active antenna is designed as composite structure to improve the filed strength sensitivity over the wide frequency range, and the multi-channel direction finder and search receiver are designed using DDS-based PLL with settling time of below 35 us to achieve short signal processing time. This system provides the capabilities of the high DF sensitivity over the wide frequency range and allows for high probability of intercept and accurate angle of arrival(AOA) estimation for agile signals. The design and performance analysis according to the external noise and modulation schemes of the CVDF system with five-element circular array are presented in detail.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2003.05a
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• pp.1073-1078
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• 2003

This paper presents the vibration characteristics of a cantilever beam in contact with a fluid using a PZT actuator and PVDF film. dynamic behaviors of a flexible beam-water interaction system are examined. The effect of the liquid level on free vibration of the composite beam in a partially liquid-filled circular cylinder is investigated. The coupled system is subject to an undisturbed boundary condition un the fluid domain. In the vibration analysis of a wetted beam. the decoupled analyses between beam and fluid have been conventionally employed by considering first the composite beam vibration in the all and secondly Performing the correction taking account for surrounding fluid effects. That is, this investigation was to look at how natural frequencies, mode shapes. and damping are affected by liquid level variations. The signals from the sensor according to the applied input voltage are digitalized and filtered in order to obtain the dynamic characteristics of the composite beam in contact with fluid. It was found that the coupled natural frequencies decreased with the fluid level for the identical composite beam due to added mass effect. In case of the free-free boundary condition, the natural frequency gently decreased at fluid water level between 20% and 80% in the first tending mode and we found out the bends of stair shape for added mass effect of the fluid.

• Transactions of the Korean Society of Mechanical Engineers
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• v.19 no.11
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• pp.2981-2994
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• 1995

In order to elucidate the effects of positive pressure gradient on flame properties, structure and stabilization, an experimental study is made on turbulent diffusion flame stabilized by a circular cylinder in a divergent duct flow. A commercial grade gaseous propane is injected from two slits on the rod as fuel. In this paper, stabilization, characteristics and flame structure are examined by varying the divergent angle of duct. Temperature, ion current and Schlieren photographs were measured. It is found that critical divergent angle is expected to be about 8 ~ 12 degree through blow-off velocity pattern to divergent angle and the positive pressure gradient influences the flame temperature, intensity of ion current and eddy structure behind the rod. With the increase of divergent angle, typical temperature of recirculation zone is low but intensity of ion current is high in shear layer behind rod. Energy distributions of fluctuating temperature and ion current signals turn up low frequency corresponding to large scale eddies but high frequency corresponding to small scale eddies as well as low with the increase of divergent angle. Therefore the flame structure becomes a typical distributed-reacting flame.

• Smart Structures and Systems
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• v.17 no.1
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• pp.31-43
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• 2016

Applications of ultrasonic tomography to concrete structures have been reported for many years. However, practical and effective application of this tool for nondestructive assessment of internal concrete condition is hampered by time consuming transducer coupling that limits the amount of ultrasonic data that can be collected. This research aims to deploy recent developments in air-coupled ultrasonic measurements of solids, described in Part 1 of this paper set, to concrete in order to image internal inclusions. Ultrasonic signals are collected from concrete samples using a fully air-coupled (contactless) test configuration. These air coupled data are compared to those collected using partial semi-contact and full-contact test configurations. Two samples are considered: a 150 mm diameter cylinder with an internal circular void and a prism with $300mm{\times}300mm$ square cross-section that contains internal damaged regions and embedded reinforcement. The heterogeneous nature of concrete material structure complicates the application and interpretation of ultrasonic measurements and imaging. Volumetric inclusions within the concrete specimens are identified in the constructed velocity tomograms, but wave scattering at internal interfaces of the concrete disrupts the images. This disruption reduces defect detection accuracy as compared with tomograms built up of data collected from homogeneous solid samples (PVC) that are described in Part 1 of this paper set. Semi-contact measurements provide some improvement in accuracy through higher signal-to-noise ratio while still allowing for reasonably rapid data collection.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.5
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• pp.1050-1056
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• 2009

Heart Rate Variability(HRV) is a parameter that represents monitoring variability of time intervals between R-peak in electrocardiography. HRV serves to various applications, such as indices of autonomic functions, prediction of cardiac sudden death, assessment of stress and emotional, etc. However, as measuring R-peak in ECG needs at least 3-electrodes, and it is inconvenient for end users. In this paper, we suggested the modified laplacian electrodes for measuring HRV at one-point, which are producted by MEMS fabrication and have the two circular electrodes on the pad. For optimal position and direction, we performed an experiment that compared with pearson correlation coefficient and the amplitude of signals, between standard lead II and proposed electrodes. We analyzed the HRV parameters, such as standard deviation of the NN interval(SDNN), high frequency(HF), low frequency(LF), LF/HF ratio. The result showed that the average correlation coefficient and amplitude are 0.967 and 0.685 mVpp at the position 2. The coeffiecient correlation between the standard HRV and proposed electrode-HRV is 0.999

• Design & Manufacturing
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• v.15 no.4
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• pp.51-56
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• 2021

Piercing is the process of shearing a circular hole in sheet metal, whose high shear force makes it difficult to secure the durability of tools. In addition, uneven clearance between tools due to poor alignment of the piercing punch causes accelerated die wear and breakage of the tool. This study reviewed the feasibility of in-situ determining alignment failure during the piercing process by analyzing the signal deviation of a bolt-type piezo sensor installed inside the tool whose alignment level was controlled. Finite element analysis was performed to select the optimal sensor location on the piercing tool for sensitive detection of process signals. A well-aligned piercing process results in uniform deformation in the circumferential direction, and shearing is completed at a stroke similar to the sheet thickness. Afterward, a sharp decrease in shear load is observed. The misaligned piecing punch leads to a gradual decrease in the load after the maximum shear load. This gradual decrease is due to the progressive shear deformation that proceeds in the circumferential direction after the initial crack occurs at the narrow clearance site. Therefore, analyzing the stroke at which the maximum shear load occurs and the load reduction rate after that could detect the misalignment of the piercing punch in real-time.

• Journal of Microbiology and Biotechnology
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• v.32 no.5
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• pp.621-629
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• 2022

Bacterial outer membrane vesicles (OMVs) typically contain multiple immunogenic molecules that include antigenic proteins, making them good candidates for vaccine development. In animal models, vaccination with OMVs has been shown to confer protective immune responses against many bacterial diseases. It is possible to genetically introduce heterologous protein antigens to the bacterial host that can then be produced and relocated to reside within the OMVs by means of the host secretion mechanisms. Accordingly, in this study we sought to develop a novel platform for recombinant OMV (rOMV) production in the widely used bacterial expression host species, Escherichia coli. Three different lipoprotein signal peptides including their Lol signals and tether sequences-from Neisseria meningitidis fHbp, Leptospira interrogans LipL32, and Campylobactor jejuni JlpA-were combined upstream to the GFPmut2 model protein, resulting in three recombinant plasmids. Pilot expression studies showed that the fusion between fHbp and GFPmut2 was the only promising construct; therefore, we used this construct for large-scale expression. After inducing recombinant protein expression, the nanovesicles were harvested from cell-free culture media by ultrafiltration and ultracentrifugation. Transmission electron microscopy demonstrated that the obtained rOMVs were closed, circular single-membrane particles, 20-200 nm in size. Western blotting confirmed the presence of GFPmut2 in the isolated vesicles. Collectively, although this is a non-optimized, proof-of-concept study, it demonstrates the feasibility of this platform in directing target proteins into the vesicles for OMV-based vaccine development.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.7
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• pp.1-7
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• 2016

In this paper, the design methodology of a corrugated conical horn antenna is proposed to be obtain wide-band properties over the full range of frequencies in the Ku-band. In order to improve the properties of the corrugated conical horn antenna, such as its gain, VSWR, co-polarization to cross-polarization ratio and wide-bandwidth, two types of mode matching converters are implemented within it. One is located at the end of the circular waveguide, while the other is positioned in front of the horn-flare section. The properties of the antenna are analyzed and compared according to the position of the proposed converters through simulations. In the comparison of the antenna performance in the case where the VSWR, co-polarization to cross-polarization ratio and antenna gain over the Ku band of 12-18 GHz are less than 2, greater than 30dB and 20dB respectively, the former antenna exhibits greater stability and a wider frequency band than the latter from the viewpoint of transmitting and receiving signals simultaneously. Therefore, considering the gain, VSWR, radiation pattern and bandwidth, the horn antenna structure in which the mode matching converter is implemented inside the circular waveguide has better performance than the other.