• Title/Summary/Keyword: chlorophyll pigment

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Development of New Edible Pigments using Monascus spp.

  • Cho, Gyu-Seong;Kim, Kwangwook;Park, Won-Jong
    • The Korean Journal of Food And Nutrition
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    • v.32 no.1
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    • pp.41-49
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    • 2019
  • Carotene, xanthophyll, carotenoid anthocyan, phycopyrine, chlorophyll, and monascus pigments are used as natural coloring agents since they are more stable to human body than synthetic coloring agents. Among them, monascus pigments are a natural red pigment produced by the Monascus purpureus. For the development of edible paint using natural pigment, Monascus purpureus strain was cultured at a temperature of $35^{\circ}C$ for 15 days on a PDYA plate and liquid medium to produce a red pigment. In addition, a large amount of the red pigment was extracted from Hongkuk Koji in parallel with water extraction and ultrasonic wave extraction. At this time, the yield of ultrasonic extract was 2~4 times higher. Thus, Monascus purpureus strains, etc. were prepared by freeze-drying powder. In conclusion, natural paints made with red pigments have enabled the development of been edible paints that can be used as eco-friendly materials with good viscosity, enhanced spread ability and coloration.

Pigment Stability of Lavers Porhyra tenera Kjellman during Processing and Storage (김의 가공 저장중에 있어서의 색소의 안정도)

  • LEE Kang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.2 no.2
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    • pp.105-133
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    • 1969
  • The stability of three major pigments in lavers, namely chlorophylls, carotenoids, and phy-cobilins, during processing and storage was studied. The results of the pigment retention in fresh lavers during storage at low temperatures, of heat lability during heat treatment, and of the pigment loss during storage are summarized as follows: 1. The contents of chlorophyll a, xanthophyll (lutein+zeaxanthin), and carotene ($\alpha-+\beta-carotene$) averaged 1,525, 627, and $409{\mu}g/g$ of dry samples respectively The comparative composition of four carotenoids, lutein, $\beta-carotene$, zeaxanthin, and $\alpha-carotene$, was 35.9, 33.7, 12.2, and $5.8\%$ respectively. 2. In storage of fresh lavers at room ternperature ($20-25^{\circ}C$), 2 to $5^{\circ}C$, and $-15^{\circ}C$, the pigments generally were retained more at 2 to $5^{\circ}C$ than others while carotene only showed more retention at $-15^{\circ}C$ than at 2 to $5^{\circ}C$. Phycocyanin tended to decrease more rapidly than phyco-erythrin at low temperatures. 3. In regard to heat treatment of both dried and wet materials at 40, 60, 80, $100^{\circ}C$ respectively, the pigments were more stable at lower temperatures than at higher temperatures in both cases of materials. Carotenoids were retained more in wet materials than in the sun dried. Xanthophylls showed higher heat stability than carotenes. Phycoerythrin was considered more heat labile than phycocyanin, particularly at higher temperatures. 4. In storage of heat treated lavers for 50 days at room temperature, the retention of pigments appeared differently in accordance with the temperatures of treatment The materials treated at $60^{\circ}C$ showed better relention of pigments than those treated at the other temperatures. Degradation rate of xanthopllylls, carotenes, and phycobilins was decreased in the materials treated at higher temperatures, showing a tendency of great pigment retention even for a long period storage. Chlorophyll il was retained more in sun dried materials whereas xanthophyll and carotene were retained more in the wet. 5. In order to maintain a higher pigment stability during storage, the materials must be treated at $60^{\circ}C$ in a dryer with rapid ventilation until the moisture level reaches about $6.0\%$. In case of treatment at a higher temperature than $60^{\circ}C$, they must be treated for a short period of time to minimize pigment degradation by heat. 6. The lavers treated with the solutions of pH around 7.8 showed a higher retention of chlorophyll a. When treated with the solutions of algal extracts such as agar and alginic acid, uniform layer of laver sheets and better surface gloss of dried layers were obtained.

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Extractions of Chlorophyll from Spinach and Mate Powders and Their Dyeability on Fabrics (시금치와 마테 분말을 이용한 클로로필 추출과 직물 염색)

  • Yoo, Hye Ja;Ahn, Cheunsoon;Narantuya, Lkhagva
    • Journal of the Korean Society of Clothing and Textiles
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    • v.37 no.3
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    • pp.413-423
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    • 2013
  • Chlorophyll is an abundant pigment found in all green plants, algae, and cyanobacteria. This study uses methanol, acetone and water to extract spinach and mate powders in order to examine the possibility of dyeing animal fibers with chlorophyll without chemical alteration. It was shown that methanol extracts of spinach and mate powders can be effectively used to dye wool and silk fabrics if the extract is mixed with water by methanol:water 65:35 v/v. Compared to methanol extract, the acetone extract showed lower chlorophyll yield and lower dye uptake. Water was not an appropriate solvent for chlorophyll extraction and dyeing. Spinach powder showed a higher dye uptake than mate powder due to the higher chlorophyll content than mate powder. It is possible that the chlorophyll dyeing of wool and silk fabrics is due to the hydrogen bonding between the hydroxy amino acids in fiber and the carbonyl groups of chlorophyll. These carbonyl groups are on the heterocyclic ring and the methyl and ethyl side chains of chlorophyll.

Effect of Extractant on the Color Characteristics of Natural Colorant Extracts (천연색소의 색 특성에 미치는 추출용매의 영향)

  • Lee, Young-Hee;Park, Young-Kwang;Baek, Young-Mee;Kim, Jung-Soo;Lee, Dong-Jin;Kim, Han-Do
    • Textile Coloration and Finishing
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    • v.28 no.1
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    • pp.1-13
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    • 2016
  • Natural colorant extracts were obtained by extraction from tumeric root, gardenia seeds, mugwort and green tea using water, methanol, ethanol and acetone as extractants at room temperature for 3 hours under shaking condition(180rpm) with liquor ratio(solid:solvent; 1:100). The main pigment components of tumeric root, gardenia seeds, mugwort and green tea are known to be curcumin, crocin, chlorophyll b and epigallocatechin gallate, respectively. The effects of the kind of extractant and pH on the color characteristics of natural colorant extracts were investigated. The solubility parameters of pigment components were determined to find adequate extractant. The solubility parameters of curcumin, crocin, chlorophyll b and epigallocatechin gallate were found to be 27.85, 29.40, 19.48 and $37.97(J/cm^3)^{1/2}$. As expected, solvents that have a solubility parameter similar to that of pigment component were generally found to be effective to obtain pigment extracts having high visible absorbance(A). The extract(pigment/solvent) with high visible absorbance was generally found to have low $L^*$(lightness) and high Chroma($C^*$, purity).

Phytoplankton in the Waters of the Ieodo Ocean Research Station Determined by Microscopy, Flow Cytometry, HPLC Pigment Data and Remote Sensing (현미경, Flow Cytometer, HPLC 색소자료 및 원격탐사를 이용한 이어도 관측기지 주변수의 식물플랑크톤 연구)

  • Noh, Jae-Hoon;Yoo, Sin-Jae;Lee, Jung-Ah;Kim, Hyun-Chul;Lee, Jae-Hak
    • Ocean and Polar Research
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    • v.27 no.4
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    • pp.397-417
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    • 2005
  • Phytoplankton community structure and distribution pattern in the surface water around the Ieodo Ocean Research Station were investigated during seven cruises carried out from July, 2003 to October, 2004. Samples were analyzed using various tools including a microscope, flow cytometer, and HPLC. Satellite images were used to analyze spatio-temporal phytoplankton biomass distribution. SeaWiFS chlorophyll a (chl a) images showed that spring blooms occurred in April-May near the Ieodo Station, and these waters were under the influence of Changjiang Dilute Water during July-October. Also, during the July-October period, HPLC pigments data showed increasing zeaxanthin concentrations, a marker pigment of cyanobacteria whereas increasing concentrations of various other pigments such as fucoxanthin, peridinin, prasinoxanthia alloxanthin, 19'-hexanoyloxyfucoxanthin and chlorophyll b were noted during spring blooms. Such pigment marker data were consistent with picoplankton data analyzed by flow cytometer and nano-microplankton analyzed by microscope. The pigment-CHEMTAX method was used to drive the phytoplankton group apportioned chi a. Diatoms, chlorophytes, dinoflagellates, and cryptophytes comprised 25.8, 20.7, 15.9, and 14.1%, respectively, of the total chl a in May. Average cyanobacteria concentrations in July-October contributed 25.4% of the total concentration. This was the highest percent contribution and was followed by chlorophytes, diatoms, and prymnesiophytes. This study discusses results from various methods, similarities and differences in the results among those methods, and the application range of the results from different analytical methods. Also, the study reveals a detailed phytolpankton community structure in the waters around the Ieodo Station, and suggests future monitoring considerations in relation to cell morphology, ecology and diversity factors according to taxonomic groups.

Changes in Mineral, Pigment, Texture, Sensory Score and Microflora during Fermentation of Gat(Leaf Mustard)-Kimchi (갓김치 숙성 중의 무기질, 색소, 조직, 관능 및 미생물군의 변화)

  • 박석규;전순실
    • Food Science and Preservation
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    • v.2 no.1
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    • pp.131-138
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    • 1995
  • In order to obtain basic data for the development of Gat-Kimchi, a salted and fermented lear mustard, changes in mineral, pigment, texture, sensory score and microflora during fermentation at 5$\pm$2$^{\circ}C$ were investigated. Changes in mineral, including iron, calcium and potassium were obviously shown and their contents were markedly decreased after 14 days of fermentation. Contents of total chlorophyll and carotenoid were slowly decreased after 6 and 10 days of fermentation, respectively and ratios of chlorophyll a/b were not changed and similar to those of other cruciferous vegetable Kimchi during fermentation. Shear force of Gat-Kimchi in rheometer during fermentation was increased. The sourness and hardness(p<0.05) of Gat-Kimchi after 24 days of fermentation were significant different in sensory evaluation with no significant difference in off-flavor, color and hotness. Compared with other Kimchi, taste of Gat-Kimchi was desirably kept for 54 days of fermentation. Total viable count and lactic acid bcateria(Genus Lactobacillus) observed to be Increased in the range of 18 to 24 days and yeasts to be gradually increased during overall period of fermentation.

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Lipid Peroxidation of Ginseng Thylakoid Membrane (인삼 틸라코이드 막의 지질과 산화)

  • 양덕조
    • Journal of Ginseng Research
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    • v.14 no.2
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    • pp.135-141
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    • 1990
  • In order to elucidate the mechanism of the leaf-burning disease of ginseng (Panax ginseng C.A. Meyer), the relationships between thylakoid membrane peroxidation and chlorophyll bleaching were investigated in comparison with the ones of soybean (Glycine max L). When I measured the rate of lipid peroxidation in the thylakoids of ginseng and soybean by irradiation of light(60 w.m-2), it was identified that, the remarkably lower rate of lipid peroxidation was found in the ginseng thylakoid than the case of soybean. When lipid peroxidation of ginseng thylakoid was induced in the dark, chlorophyll contents of thylakoid was not changed. The results suggest that lipid peroxidation does not affect the chlorophyll bleaching in ginseng thylakoid. Thylakoid membrane peroxidation as well as chlorophyll bleaching was closely related with photosynthetic electron transport. But, according to the quenching experiment active oxygen species induced lipid peroxidation may be different species in the case of chlorophyll bleaching.

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Needle Life Span, Photosynthetic Pigment and Nitrogen Allocation of Picea jezoensis in Korea (우리나라 가문비나무의 침엽 수명, 광색소 및 질소 배분 특성)

  • Han, Sim-Hee;Kim, Du-Hyun;Kim, Gil Nam;Yun, Chung-Weon
    • Journal of Korean Society of Forest Science
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    • v.101 no.1
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    • pp.62-68
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    • 2012
  • We have investigated needle life span, photosynthetic pigment and nitrogen allocation pattern in the needle of Picea jezoensis in the three populations (Gyebangsan, Deogyusan and Jirisan) to find out growth environmental condition which needs the strategy development of conservation and restoration against population decline. Needle survival rate was the highest in Gyebangsan (87.0%) and the lowest in Jirisan (71.6%), and it decreased with the increase of needle age. Needle chlorophyll a and b in Deogyusan and Jirisan showed higher content in older needle, but chlorophyll content in Gyebangsan was the highest in 2-year-old needle. Carotenoid content was the highest in the needle in Gyebangsan, and it increased along with needle age. Chlorophyll a/b ratio showed the highest value in Jirisan and the lowest value in Gyebangsan. Chlorophyll/carotenoid ratio decreased with needle age, Needle nitrogen content was the highest in Deogyusan (1.51%) and the lowest in Gyebangsan (1.40%), and the older needle had the lower content. In contrast, the highest chlorophyll/nitrogen ratio represented the oldest needle.

Effects of light-emitting diodes on protoplast regeneration from gametophytic cells of the commercial kelp Undaria pinnatifida (Laminariales, Phaeophyceae)

  • Avila-Peltroche, Jose;Won, Boo Yeon;Cho, Tae Oh
    • ALGAE
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    • v.37 no.2
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    • pp.163-174
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    • 2022
  • Light-emitting-diodes (LEDs) are a lighting source useful for the precise evaluation of light quality effect on biological systems. Despite the importance of light spectra on the regeneration of land plant protoplasts ("naked cells"), this factor has not been tested yet on protoplasts from multicellular algae. This study reports on the effects of pure primary colors (red, blue, and green), dichromatic (red plus blue, RB, 1 : 2) and white LEDs on protoplast regeneration from male and female Undaria pinnatifida gametophytes. We also evaluated the effect of different light spectra on pigment composition (chlorophyll a, chlorophyll c, and fucoxanthine), and the light intensities under the best condition on the regeneration process. In the early stages, blue or RB LEDs increased the percentage of dividing female protoplasts, whereas red, blue, and RB LEDs enhanced that of dividing male protoplasts. In the later stages, RB LEDs showed a positive effect only on the percentage of multiple rhizoid-like protrusions (male gametophyte). They also increased the final area of both regenerated gametophytes. The LEDs did not affect pigment composition in female gametophytes. In male gametophytes, in contrast, they reduced chlorophyll c, while blue, RB, and green LEDs decreased fucoxanthin. Under RB LEDs, the optimal light intensity was 80 µmol photons m-2 s-1 for female gametophytes and 40 to 60 µmol photons m-2 s-1 for male gametophytes. Our results suggest that dichromatic LED illumination (red-blue) improves regeneration of U. pinnatifida gametophyte-isolated protoplasts. Thus, dichromatic LEDs might a suitable light source for enhancing protoplast regeneration in brown seaweeds.

Estimating chlorophyll-A concentration in the Caspian Sea from MODIS images using artificial neural networks

  • Boudaghpour, Siamak;Moghadam, Hajar Sadat Alizadeh;Hajbabaie, Mohammadreza;Toliati, Seyed Hamidreza
    • Environmental Engineering Research
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    • v.25 no.4
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    • pp.515-521
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    • 2020
  • Nowadays, due to various pollution sources, it is essential for environmental scientists to monitor water quality. Phytoplanktons form the end of the food chain in water bodies and are one of the most important biological indicators in water pollution studies. Chlorophyll-A, a green pigment, is found in all phytoplankton. Chlorophyll-A concentration indicates phytoplankton biomass directly. Therefore, Chlorophyll-A is an indirect indicator of pollutants, including phosphorus and nitrogen, and their refinement and control are important. The present study, Moderate Resolution Imaging Spectroradiometer (MODIS) satellite images were used to estimate the chlorophyll-A concentration in southern coastal waters in the Caspian Sea. For this purpose, Multi-layer perceptron neural networks (NNs) were applied which contained three and four feed-forward layers. The best three-layer NN has 15 neurons in its hidden layer and the best four-layer one has 5 in each. The three- and four- layer networks both resulted in similar root mean square errors (RMSE), 0.1($\frac{{\mu}g}{l}$), however, the four-layer NNs proved superior in terms of R2 and also required less training data. Accordingly, a four-layer feed-forward NN with 5 neurons in each hidden layer, is the best network structure for estimating Chlorophyll-A concentration in the southern coastal waters of the Caspian Sea.