• Horticultural Science & Technology
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• v.18 no.6
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• pp.823-826
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• 2000

This study was carried out to investigate the effect of plant growth retardants (daminozide, chlormequat, hexaconazole, uniconazole, paclobutrazole) and fungicides of triazole chemical (microbutanil, difenoconazole, terbuconazole, bitertanol) on the rooting of 'New Guinea' impatiens cuttings. In both 'Papete' and 'Anguilla', the formation of adventitious roots was promoted by all concentrations of chlormequat, hexaconazole, uniconazole, and paclobutrazole treated. More adventitious roots were formed with hexaconazole, uniconazole, and paclobutrazole than with chlormequat. Length of the longest root and rooting zone decreased at higher concentrations of chlormequat, hexaconazole, uniconazole, and paclobutrazole in 'Papete', while 'Anguilla' showed less responses. In contrast with plant growth retardants, the formation of adventitious roots was remarkably promoted by fungicides of triazole chemical such as microbutanil, difenoconazole, terbuconazole, and bitertanol in 'Pepete' and 'Martinique' impatiens, but was not in 'Pagopago'. However, length of the longest root and rooting zone showed more sensitive response in 'Martinique' and 'Pagopago' than in 'Pepete'. These results suggest that fungicides of triazole chemical can be effectively used for rooting promotion of impatiens cuttings.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.564-567
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• 2001

This experiment investigated the dwarfing effect of daminozide and chlormequat to produce high quality of spray chrysanthemum. Long neck of cut-chrysanthemum break easily. Peduncle length of the cultivar 'Biarittz' and 'Vyking' were retarded by the foliar spray treatment of $1500mg{\cdot}L^{-1}$ daminozide. Daminozide application did not affect plant growth of 'Biarittz' and 'Vyking'. However, the number of days to flowering was delayed by 3 days by daminozide application of 'Biarittz'. Length of cut flowers of 'Vyking' was decreased by the application of daminozide, while chlormequat did not affect dwarfing of peduncle length in spray chrysanthemum.

• Korean Journal of Environmental Agriculture
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• v.39 no.4
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• pp.368-374
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• 2020

BACKGROUND: The residual analysis of polar pesticides has remained a challenge. It is even more difficult to simultaneously analyze multiple polar pesticides. Diquat, paraquat, and chlormequat are typical examples of highly polar pesticides. The existing methods for the analysis of diquat, paraquat and chlormequat are complex and time consuming. Therefore, a simple, quick and effective method was developed in the represent study for simultaneous analysis of diquat, paraquat and chlormequat in animal products, meat and fat using UPLC-MS/MS. METHODS AND RESULTS: Sample extraction was carried out using acidified acetonitrile and water and re- extracted with acidified acetonitrile and combine the extracts followed by centrifugation. The extract was then cleaned up with a HLB cartridge after reconstitution with acidic acetonitrile and water. The method was validated in quintuplicate at three different concentrations. The limits of detection (LOD) and quantification (LOQ) were 0.0015 and 0.005 mg/L, respectively. Matrix suppression effect was observed for all of the analytes. A seven point matrix matched calibration curve was constructed for each of the compound resulted excellent linearity with determination coefficients (R2) ≥ 0.991. Accuracy and precision of the method was calculated from the recovery and repeatability and ranged from 62.4 to 119.7% with relative standard deviation less than 18.8%. CONCLUSION: The recovery and repeatability of the developed method were in the acceptable range according to the Codex Alimentarius guideline. The developed method can be applied for the routine monitoring of diquat, paraquat, and chlormequat in animal products, meat and fat.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.574-578
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• 2001

The effects of seed soaking (8 and 24 hours) and foliar spray of plant growth retardants (daminozide, chlormequat and uniconazole) on growth control in zinnia were investigated. For seed soaking of 'Dream Land Rose', the changes in plant height was not affected by seed soaking time (8 and 24 hours), while it was severely suppressed by high concentration of chemicals tested. An application of $125mg{\cdot}L^{-1}$ uniconazole was most effective for plant height suppression. Flowering was promoted by all treatments of chemicals. However, for 'Dream Land Scarlet', plant height control at 24 hours seed soaking showed more effective than 8 hours, and all treatments of chemicals decreased plant height. Uniconazole gave the best result for plant height control. Flowering tended to be hastened by the treatment of chemicals. For foliar spray, plant height was remarkably decreased at high concentration of uniconazole. Flowering was promoted by chlormequat and uniconazole in both 'Dream Land Rose' and 'Dream Land Scarlet'. Also, daminozide decreased number of petals.

• FLOWER RESEARCH JOURNAL
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• v.19 no.3
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• pp.127-138
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• 2011

Four different plant growth retardants (PGRs), paclobutrazol, flurprimidol, daminozide, and chlormequat, were applied to potted Saxifraga rosacea 'Kumoma' and 'Kumoma-Gusa' plants for control of the growth and flowering. Paclobutrazol (10, 20, 40, $80mg{\cdot}L^{-1}$), flurprimidol (5, 10, 20, $40mg{\cdot}L^{-1}$), daminozide (500, 1000, 2000, $4000mg{\cdot}L^{-1}$), and chlormequat (50, 100, 200, $400mg{\cdot}L^{-1}$) were applied to the plants by a foliar spray or drenching. In 'Kumoma', application of $40mg{\cdot}L^{-1}$ paclobutrazol by a foliar spray or drenching reduced plant height by 12.5 and 12.6 cm, and flower length by 3.4 and 3.3 cm, respectively. On the other hand, in 'Kumoma-Gusa', drenching of paclobutrazol reduced plant height by 10.7 to 12.6 cm and flower length by 2.0 to 3.9 cm with increasing concentration, but the number of florets almost fell to 20 as compared to 40.5 in the control. 'Kumoma-Gusa' plants drenched with $80mg{\cdot}L^{-1}$ paclobutrazol and sprayed with $40mg{\cdot}L^{-1}$ flurprimidol had the shortest heights of 10.7 and 9.9 cm, and floral length of 2.0 and 1.5 cm, respectively. A flurprimidol drenching at $40mg{\cdot}L^{-1}$ delayed the harvest by 3-13 days as compared to the control and the smallest number of florets, 15.6, was observed in this treatment. In both cultivars, chlormequat and daminozide did not effectively influence the growth and flowering. However, number of florets increased to more than 41 at all concentrations and up to 63, the greatest floret number, with chlormequat drench in 'Kumoma-Gusa'. These results demonstrated that over $40mg{\cdot}L^{-1}$ of paclobutrazol or 5 to $20mg{\cdot}L^{-1}$ of flurprimidol could be used as PGRs to control the growth of floral length and flowering for improving potted plant quality in S. rosacea 'Kumoma' and 'Kumoma-Gusa'.

• Horticultural Science & Technology
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• v.18 no.6
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• pp.827-833
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• 2000

This study was carried out to investigate the effect of plant growth retardants (daminozide, chlormequat, uniconazole, paclobutrazole) and fungicides of triazole chemical (hexaconazole, microbutanil, difenoconazole, terbuconazole, bitertanol) on the growh of 'New Guinea' impatiens. Plant height and canopy were decreased by all kinds of plant growth retardants tested in both 'Anguilla' and 'Papete'. Especially, uniconazole and paclobutrazole were most effective in inhibition of top growth. However, the content of chlorophyll increased at all treatments of plant growth retardants and stem diameter tended to be increased at the highest concentration of all kinds of plant growth retardants tested. The results in all cultivars tested, with the experiment of triazole fungicides, were similar to the results of experiment with plant growth retardants. These results suggest that fungicides of triazole chemical such as hexaconazole, microbutanil, difenoconazole, terbuconazole and bitertanol can be used for the promotion of quality in potted 'New Guinea' impatiens.

• Plant Biotechnology Reports
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• v.4 no.1
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• pp.9-13
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• 2010

Guggulsterone, a hypolipidemic natural agent, is produced in resin canals of the plant Commiphora wightii. In this study, the stimulatory effects of growth retardants [ALAR (N,N-dimethylaminosuccinamic acid) and CCC (chlormequat chloride)] and fungal elicitor on guggulsterone accumulation in cell cultures of C. wightii are reported. CCC at $1\;mg\;l^{-1}$ enhanced guggulsterone content (${\sim}123\;{\mu}g\;l^{-1})$ when added on the fifth day after inoculation, while ALAR at $2.5\;{\mu}g\;l^{-1}$ increased guggulsterone content (${\sim}116\;{\mu}g\;l^{-1}$) when added on the tenth day. In a two-stage fed-batch process, combined treatment with fungal elicitor and growth retardant caused a significant increase (${\sim}353\;{\mu}g\;l^{-1}$) in guggulsterone content in cell cultures after 17 days of growth. This represents an approximately fivefold increase over the guggulsterone contents in initial cultures of this plant.

• Asian Journal of Turfgrass Science
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• v.12 no.3
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• pp.173-182
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• 1998

This study was conducted to evaluate the effect of plant growth inhibitors on "Penncross" creeping bentgrass in the green of golf course for increasing the summer quality. Chlormequat chloride (C) 250$\times$, 500$\times$, 1000$\times$, daminozide (D) 50$\times$, 100$\times$, 200$\times$, paclobutralzol (P) 1000$\times$, 2000$\times$, 4000$\times$, trinexpac-ethyl (T) 1000$\times$, 2000$\times$, 4000$\times$, uniconazole (U) 25$\times$, 50$\times$, 100$\times$ with three concentrations, respectively, were applied to creeping bentgrass. The leaf length, leaf width, and internode length of stolon were inhibited by all plant growth retardants applied as compared to that of control in which D-50, T-2000, and trinexpac-ethyl were the most effective among treatments on the inhibition of leaf length and internode length of stolen, respectively. Leaf thickness increased more in the treatments of C-250, D-50. T-2000 and T-4000 than any other treatments. On the other hand, shoot density which was represented by number of leaves per unit area was found to be the highest in P-2000 and P-4000 treatment. In this case, all plant growth retardants with no significant differences showed increase of 15-20% in density as compared to control. Total dry weight increment was higher in P-2000 and P-4000 while leaf dry weight increment was higher in T-4000. and U-25 and 50 than other treatments. According to the visual evaluation for the purpose of turf quality measurement, it was found that paclo-butralzol, trinexpac-ethyl and uniconazole were not suitable for green maintenance and extension because of making leaves to yellow. In conclusion, it was postulated that C-250, D-50, and T-4000 had possibility to use practically in the green area of golf course.lf course.

• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.335-340
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• 1997

100% root formation in in vitro cultures was observed regardless of kind and levels of auxin used and explant source. The number of roots/explant was increased in 0.5~1.0 mg/L IAA treatment. Thicker roots were observed with the addition of 9% sucrose compared with medium containing lower sucrose concentrations. Paclobutrazol and chlormequat had no effect on tuberization of formed roots but slightly increased the number of root. In in vivo rooting, soaking of regenerated shoot cuttings to 100 mg/L IBA for 15 to 60 minutes was found effective. Treatment of 0.1% IBA rooting powder and planting in rooting medium composred of vermiculite(1) : perlite(1) gave 100% rooting and survival.

• Korean Journal of Agricultural Science
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• v.18 no.1
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• pp.1-9
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• 1991

This experiment was aimed to develop possible methods to control the maturity of grape berries through the application of exogenous plant growth substances and physical treatments such as defoliation or girdling. 1. Chlormequat and paclobutrazol increased anthocyanin but did not affect soluble solids contents and maturity. Girdling enhanced maturation and solids accumulation whereas defoliation delayed maturity. Solids content of berries in defoliation treatment did not reach to the level of other treatments even when fully ripened. 2. Ripening of grapes is greatly delayed for 20 to 30 days by the application of auxins (2,4-D and fenoprop) compared to the untreated control. Uneven ripening of berries in those clusters was observed when the concentration of auxin was over 50 ppm. Thus, about 30% of berries remained green until the normal berries were overripened. 3. Gibberellin did not affect the maturity of grape berries but maturity was greatly delayed when GA was applied with auxins. Also, uneven coloration between berries was observed such as in the application of auxin alone. 4. Ethephon application combinded with calcium at veraison showed no effect on berry ripening but increased anthocaynin contents. It can be concluded that harvest time of 'Campbell Early' grapes can be effectively extended by delaying the maturity through the application of auxin.