Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Development of a Simultaneous Analytical Method for Diquat, Paraquat and Chlormequat in Animal Products Using UPLC-MS/MS

  • Cho, Il Kyu (Eco-Friendly Agri-Bio Research Center, Jeonnam Bioindustry Foundation) ;
  • Rahman, Md. Musfiqur (Eco-Friendly Agri-Bio Research Center, Jeonnam Bioindustry Foundation) ;
  • Seol, Jae Ung (Eco-Friendly Agri-Bio Research Center, Jeonnam Bioindustry Foundation) ;
  • Noh, Hyun Ho (Residual Agrochemical Assessment Division, Department of Agro-Food Safety & Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jo, Hyeong-Wook (Hansalim Agro-Food Analysis Center, Hankyong National University Industry Academic Cooperation Foundation) ;
  • Moon, Joon-Kwan (School of Applied Science in Natural Resources and Environment, Hankyong National University)
  • Received : 2020.11.03
  • Accepted : 2020.11.30
  • Published : 2020.12.31
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Abstract

BACKGROUND: The residual analysis of polar pesticides has remained a challenge. It is even more difficult to simultaneously analyze multiple polar pesticides. Diquat, paraquat, and chlormequat are typical examples of highly polar pesticides. The existing methods for the analysis of diquat, paraquat and chlormequat are complex and time consuming. Therefore, a simple, quick and effective method was developed in the represent study for simultaneous analysis of diquat, paraquat and chlormequat in animal products, meat and fat using UPLC-MS/MS. METHODS AND RESULTS: Sample extraction was carried out using acidified acetonitrile and water and re- extracted with acidified acetonitrile and combine the extracts followed by centrifugation. The extract was then cleaned up with a HLB cartridge after reconstitution with acidic acetonitrile and water. The method was validated in quintuplicate at three different concentrations. The limits of detection (LOD) and quantification (LOQ) were 0.0015 and 0.005 mg/L, respectively. Matrix suppression effect was observed for all of the analytes. A seven point matrix matched calibration curve was constructed for each of the compound resulted excellent linearity with determination coefficients (R2) ≥ 0.991. Accuracy and precision of the method was calculated from the recovery and repeatability and ranged from 62.4 to 119.7% with relative standard deviation less than 18.8%. CONCLUSION: The recovery and repeatability of the developed method were in the acceptable range according to the Codex Alimentarius guideline. The developed method can be applied for the routine monitoring of diquat, paraquat, and chlormequat in animal products, meat and fat.

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References

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