• BMB Reports
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• 제45권8호
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• pp.482-487
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• 2012

To identify the novel inhibitors of endoplasmic reticulum stress-induced cell death, we performed a high throughput assay with a chemical library containing a total of 3,280 bioactive small molecules. Cyclosporine A and bromocriptine were identified as potent inhibitors of thapsigargiin-induced cell death (cut-off at $4{\sigma}$ standard score). However, U74389G, the potent inhibitor of lipid peroxidation had lower activity in inhibiting cell death. The inhibition effect of cyclosporine A and bromocriptine was specific for only thapsigargin-induced cell death. The mechanism of inhibition by these compounds was identified as modification of the expression of glucose regulated protein-78 (GRP-78/Bip) and inhibition of phosphorylation of p38 mitogen activated protein kinase (MAPK). However, these compounds did not inhibit the same events triggered by tunicamycin, which was in agreement with the cell survival data. We suggest that the induction of protective unfolded protein response by these compounds confers resistance to cell death. In summary, we identified compounds that may provide insights on cell death mechanisms stimulated by ER stress.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.4106-4106
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• 2001

A method to discriminate human gallstones by nea. infrared(NIR) spectrometry using a soft independent modeling of class analogy (SIMCA) has been studied. The fifty NIR spectra of gallstones in the wavenumber range from 4500 to 10,000 cm$\^$-1/ were measured. The forty samples were classified to three classes, cholesterol stone, calcium bilirubinate stone and calcium carbonate stone according to the contents of major components in each gallstone. The training set which contained objects of the different known class was constructed using forty NIR spectra and the test set was made with ten different gallstone spectra. The number of important principal components(PCs) to describe the class was determined by cross validation in order to improve the decision criterion of the SIMCA for the training set. The score plots of the class training set whose objects belong to the other classes were inspected. The critical distance of each class was computed using both the Euclidean distance and the Mahalanobis distance at a proper level of significance(${\alpha}$). Two methods were compared with respect to classification and their robustness towards the number of PCs selected to describe different classes.

• 여성건강간호학회지
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• 제11권1호
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• pp.52-57
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• 2005

Purpose: This study was to examine the relationships between knowledge about osteoporosis and cognitive factors in middle-aged women. Method: The subjects were 293 middle-aged women. Data collection was performed by using a questionnaire that included an Osteoporosis Knowledge Test, Osteoporosis Self-Efficacy Scale and Osteoporosis Health Belief Scale developed by Kim, Horan & Gendler(1991). Data was analyzed using the SPSS Win 10.0 program for descriptive statistics, and the Pearson correlation coefficient. Result: The mean osteoporosis knowledge was 14.0. The subscale means of osteoporosis health belief variables were: susceptibility 15.9, seriousness 17.2, benefits of exercise 22.9, benefits of calcium 21.4, barriers to exercise 20.9, barriers to calcium 22.5, and health motivation 18.6. The mean osteoporosis self-efficacy was 39.3 with a moderate score. There were significantly positive correlations among knowledge about osteoporosis, health belief, and self-efficacy about osteoporosis. Conclusion: Osteoporosis knowledge, health belief, and osteoporosis self-efficacy are related. Therefore, knowledge through education is an important factor in behavioral changes and it contributes to increase the health belief and self-efficacy of osteoporosis.

• Restorative Dentistry and Endodontics
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• 제28권1호
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• pp.57-63
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• 2003

The purpose of the present study were to evaluate microleakage of a fourth generation dentin-bonding agent following a walking bleaching treatment, to determine the effect of temporary postbleaching dressing with calcium hydroxide on microleakage and to investigate the effect of delayed intracoronal restoration on microleakage. The results of this study were as follows : 1. Bleached groups showed more microleakage than unbleached group 2. Immediately restored group following bleaching procedure showed the highest microleakage score. 3. One-week delayed restorations showed less microleakage but there were no statistically significant difference between group II and III. 4. Provisional dressing with calcium hydroxide had no influence on microleakage. It is necessary to know the time that has elapsed from the bleaching treatment to the restoration procedure to achieve optimal seal, as well as to reduce the risk of microleakage in adhesive restoration.

• 동아시아식생활학회지
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• 제8권3호
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• pp.280-288
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• 1998

The purpose of this study was to evaluate the nutrient and food intake status in underweight female college students living in Seoul. 126 underweight(BMI<20) and 74 normal weight (20$\leq$BMI<25) students were asked for their daily food intake and eating habits by using 3-day food records and questionnaire. We analyzed the amounts of the nutrient intakes and food groups as the subjects. The mean of height and weight were 162.1cm and 49.0kg in underweight and 160.6cm and 54.9kg in normal weight subjects. The mean energy, iron, and vitamin A intakes among the students were smaller than RDA for Koreans. The mean calcium(p<0.01), sodium(p<0.05), and vitamin B$_2$(p<0.05) intakes in underweight subjects were significantly higher than those in normal weight subjects. Total food intakes of underweight and normal weight students were 1235.8g and 1078.1g, respectively. The mean of the pulse group in the underweight was significantly higher than that in normal group(p<0.01) Frequently consumed food or dish items of the subjects were Kimchi, cooked rice, and milk. In the relation between BMI and dietary factors, the BMI was found to have a significant negative correlation with calcium intake(p<0.05), pulse intake(p<0.01) and eating habit score(p<0.01) Eating habit scores about quantity of food and lipid intake showed a significant difference between underweight and normal students.

• 동아시아식생활학회지
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• 제11권1호
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• pp.82-88
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• 2001

The purpose of this study was to examine the effect of age, education and residence on food consumption and nutrient Intakes of older women living alone. In-home interviews were conducted using the health habits and food frequency questionnaire for one hundred twenty free living, healthy women who were between the ages of 60 and 87 years. Analysis of variance was used to assess the effect of three independent variables on food consumption and nutrient intakes. Food groups eaten at every meal were examined on 5-point scale, and consumption scores were compared with groups according to age and education. The meat consumption of younger and more highly educated respondents were significantly( p<0.05) higher. The oldest Participants had higher consumption frequencies for cereals and grain food categories. but lower consumption frequencies for vegetables and fruits ( p<0.05) The dietary assessment data showed that the energy and Protein intakes of subjects were 84.8% and 85.1% of RDAs, respectively. Vitamin A and calcium intakes showed a significant (p<0.05) difference among the three age groups More highly educated respondents had higher intakes of calcium and phosphorus than those respondents with less education.

• 대한지역사회영양학회지
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• 제16권6호
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• pp.716-729
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• 2011

This study aimed to plan nutrition support programs for the elderly living alone whose nutrition status were seriously concerned, conducted seven stages nutrition intervention program on a trial basis, and evaluated the effectiveness of the program of the Elderly Nutrition Support Project. Subjects were selected for personalized nutrition management based on nutritional risk score and nutrition intervention were tailored to the problems occurred. The elderly nutrition support program targets were 44 senior citizens who lived alone with low income. The 33 (as Type 1) of the subjects with whom milk, tofu, seaweed, eggs, black beans have been supported, and also provide nutrition education, and the rest 11 persons (as Type 2) to whom food was not supported but provide nutrition education programs. As a result, all subjects showed that compared with pre and post program implementation, their daily exercise time and milk and protein consumption level were increased and some improvement was observed regular meals consumption and low-salt diets. Their nutrient intake level such as calories, protein, calcium, iron improved after implementation. In addition, NSL DETERMINE scores significantly improved from 13.21 to 7.24 in Type 1 and 11.27 to 9.91 in Type 2. As positive dietary behavioral changes were observed as in that they purchased more protein and calcium rich foods.

• 한국생활과학회지
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• 제23권4호
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• pp.743-757
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• 2014

The purpose of this study was to compare nutrient intakes, blood lipids and bone density of male(n=69) and female(n=71) according to the obesity index by %Fat. The average energy intakes of obesity group was higher than normal group in male & female(p<0.001). The protein and fat intakes among three energy nutrients for male were higher than normal group(p<0.05~0.01), and carbohydrate intake of obesity group in female was higher than the other groups. TG, TC/HDL, blood glucose, blood pressure in male were higher than female(p<0.01~0.001), but HDL in male was lower than female. TC/HDL and diastolic blood pressure of obesity group were higher than normal group in male(p<0.05), but had not significant in female. Blood glucose in male was higher than female(p<0.001). T-score of forearm(=-1.26) and calcaneus(=0.42) for female were lower than male(=-0.63, 0.83), and T-score of calcaneus for obesity group(=1.03) in female was higher than normal group(=0.10). The T-score of forearm for obesity group in female was higher than the other group, but was not significant. These results suggest that ratio among three energy nutrients was kept balance for obesity group, especially, fat must be reduced in male than female, carbohydrate will be reduced in female than male to prevent obesity. Nutritional education for treatment obesity to prevent hyperlipidemia and arteriosclerosis is important for male too. T-score of forearm was lower than calcaneus in female, so exercise with arm than leg would be required to accumulate calcium in bone and to increase muscle to prevent osteoporosis.

• 한국식품영양과학회지
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• 제43권10호
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• pp.1510-1518
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• 2014

본 연구에서는 구연산칼슘 투여가 papain으로 유도된 골관절염 동물모델에 미치는 영향을 살펴보았다. 8주령의 C57 BL/6J 마우스의 우측 슬관절강에 papain $6{\mu}L$를 투여하여 골관절염을 유발하였다. 실험군은 각 군당 8마리씩 체중이 고르게 배정하여 총 5군으로 나누었다. 정상군은 우측 슬관절강에 생리식염수 $6{\mu}L$를 주사하였고, 대조군, 약물투여군, 구연산칼슘 투여군은 papain $6{\mu}L$로 골관절염을 유발하였다. 정상군과 대조군은 0.2 mL의 생리식염수를, 약물투여군은 diclofenac sodium 2 mg/kg/bw를, 구연산칼슘 투여군은 150, 300 mg/kg/bw를 28일간 1일 매일 정해진 시간에 1회 경구 투여하였다. 실험기간 동안 체중을 측정하였으며, 실험 종료일에는 부검하여 슬관절의 병리조직학적 관찰을 하였고 관절연골 내 proteoglycan 함유율, 골관절염 지수의 변화, 혈액 내 TNF-${\alpha}$, IL-$1{\beta}$, IL-6 함량을 측정하였다. 체중은 실험기간 동안 모든 개체 간의 유의적인 차이는 없었다. 혈액 내 TNF-${\alpha}$, IL-$1{\beta}$, IL-6 함량은 papain 투여 대조군이 정상군에 비해 유의적으로 증가하였으며 약물 및 구연산칼슘 투여군은 대조군보다 유의성 있게 낮았다. 관절연골 내 proteoglycan의 함유율은 약물 및 구연산칼슘 투여군이 papain 투여 대조군보다 유의성 있게 높았다. Hematoxylin과 eosin 염색 관찰 결과, 골관절염이 유발된 마우스에 구연산칼슘을 경구 투여하였더니 관절연골의 파괴와 골 침식 등 연골의 변성이 약물 투여군과 유사하게 나타났다. 이와 같은 결과는 구연산칼슘이 papain으로 유도된 골관절염에서 연골의 변성을 줄이고 염증을 억제함으로써 골관절염 치료에 효과가 있은 것으로 사료되며, 추후 더 자세한 연구가 필요하다.

• 대한영양사협회학술지
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• 제10권1호
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• pp.13-24
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• 2004

This study was designed to examine fast food consumption, nutritional knowledge, food behavior and dietary intake of university students and to investigate if there were differences in these variables by fast food consumption. The questionnaire was administered to university students in Daejeon. Data(n=269) was analyzed using $x^2$-test and analysis of variance. Subjects were mostly female(62%) and freshmen or sophomores(86%). Based on the frequency of fast food consumption, subjects were categorized into non-users(27.9%), users(<2 times/week, 42%) and frequent users($\geq$2 times/week, 30.1%). Those who used fast foods(n=194) consumed the foods 7.5 times per month, on the average. Subjects scored 15.6 out of 20 on a nutritional knowledge scale, showing the moderate level of knowledge. When examined by fast food use, the nutritional knowledge score was 15.5 for non-users, 16.1 for users, and 15.0 for frequent-users(p<0.05). Only two items, regarding 'fat type(animal, plant) and health' and 'importance of having breakfast', were significantly different by fast food consumption, with user group and non-users scoring higher than frequent-users(p<0.05). Food behaviors, measured by 20 items, were not desirable, with mean scores of 51.5(possible score: 20-100). Subjects showed problems in eating meals regularly, eating a variety of foods, eating breakfast, and consumption of some food groups(vegetables, fruits, and proteins). Fast food non-users showed more desirable food behaviors than users or frequent-users, such as having processed foods (p<0.001) and eating-out less frequently(p<0.01). Dietary intake data showed that some nutrient intakes, including energy, calcium, iron, zinc and folic acid were less than 75% of the RDA. Index of nutritional quality(INQ) was adequate except for calcium and zinc. Compared to non-users or user group, frequent-users of fast foods consumed higher amounts of lipids(p<0.05), and had lower INQ for calcium(p<0.01). This study described the status of fast food consumption, nutritional knowledge, food behavior of university students, and provided some baseline data for planning nutrition education for university students.