• Title/Summary/Keyword: cadaver

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Morphometric Study of the Lumbar Posterior Longitudinal Ligament

  • Lee, Sang Beom;Chang, Jae Chil;Lee, Gwang Soo;Hwang, Jae Chan;Bae, Hack Gun;Doh, Jae Won
    • Journal of Korean Neurosurgical Society
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    • v.61 no.1
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    • pp.89-96
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    • 2018
  • Objective : Morphometric data for the lumbar posterior longitudinal ligament (PLL) was investigated to identify whether there is a difference in the morphometry of the PLL of the lumbar spine at each level with respect to the pattern of intervertebral disc displacement. Methods : In 14 formalin-fixed adult cadavers (12 males and 2 females), from L1 to L5, the authors measured the width and height of the PLL and compared them with other landmarks such as the disc and the pedicle. Results : Horizontally, at the upper margin of the disc, the central portion of the superficial PLL covered 17.8-36.9% of the disc width and the fan-like portion of the PLL covered 63.9-76.7% of the disc width. At the level of the median portion of the disc, the PLL covered 69.1-74.5% of the disc width. Vertically, at the level of the medial margin of the pedicle, the fan-like portion of the PLL covered 23.5-29.9% of the disc height. In general, a significant difference in length was not found in the right-left and male-female comparisons. Conclusion : This study presents the morphometric data on the pattern of intervertebral disc displacement and helps to improve the knowledge of the surgical anatomy of the lumbar PLL.

A Clinical Analysis of the Anatomy of the Superficial Temporal Vessels (얕은관자혈관의 임상 해부학적 분석)

  • Nam, Su Bong;Choi, Chi Won;Hwang, So Min;Kim, Sang Ho;Bae, Yong Chan
    • Archives of Plastic Surgery
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    • v.32 no.2
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    • pp.214-218
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    • 2005
  • The anatomy and distribution of the superficial temporal vessels are known to follow regular patterns, with few exceptions in previous studies. But these previous studies of the anatomy and distribution of the superficial temporal vessels were based, only on the cadaver studies. Authors evaluated the anatomy of these vessels in the operative field of the living body from December 1997 to June 2001, The superficial temporal vessels were surgically exposed from the zygomatic arch extending to the superior temporal line through a preauricular incision in 18 patients(20 cases), who underwent reconstructive surgery using these vessels in the operative field. The authors measured and analyzed; the distribution, branching and diameters of the superficial temporal vessels. The results were obtained as follows; 1.In 19 cases, STV(superficial temporal vein) runs posterior to STA(superficial temporal artery) at the upper border of the zygomatic arch. 2. There was no frontal or parietal branches in 2 cases and vena comitante existed along with STA in one case. 3. The external diameter of STA and STV was measured at the lower border of the zygomatic arch. The external diameter of STA ranged from 1.5 mm to 3.0 mm(average 2.1 mm) and those of STV ranged from 1.3 mm to 3.5 mm (average 2.0 mm). This study can be helpful in the reconstructive surgery using the superficial temporal vessels, for the results of our study are based on the true anatomy of the living body.

Identification of an Entomopathogenic Bacterium, Serratia sp. ANU101, and Its Hemolytic Activity

  • Kim, Yong-Gyun;Kim, Keun-Seob;Seo, Ji-Ae;Shrestha, Sony;Kim, Hosanna-H.;Nalini, Madanagopal;Yi, Young-Keun
    • Journal of Microbiology and Biotechnology
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    • v.19 no.3
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    • pp.314-322
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    • 2009
  • Four different bacterial colonies were isolated from an old stock of an entomopathogenic nematode, Steinernema monticolum. They all showed entomopathogenicity to final instar larvae of beet armyworm, Spodoptera exigua, by hemocoelic injection. However, they varied in colony form, susceptibility to antibiotics, and postmortem change of the infected host insects. Biolog microbial identification and 16S rDNA sequence analyses indicate that these are four different species classified into different bacterial genera. Owing to high entomopathogenicity and a cadaver color of infected insect host, Serratia sp. was selected as a main symbiotic bacterial species and analyzed for its pathogenicity. Although no virulence of Serratia sp. was detected at oral administration, the bacteria gave significant synergistic pathogenicity to fifth instar S. exigua when it was treated along with a spore-forming entomopathogenic bacterium, Bacillus thuringiensis. The synergistic effect was explained by an immunosuppressive effect of Serratia sp. by its high cytotoxic effect on hemocytes of S. exigua, because Serratia sp. caused septicemia of S. exigua when the bacterial cells were injected into S. exigua hemocoel. The cytotoxic factor(s) was present in the culture medium because the sterilized culture broth possessed high potency in the cytotoxicity, which was specific to granular cells and plasmatocytes, two main immune-associated hemocytes in insects.

Comparison of Three Different Techniques in Cervical Transpedicular Screw Insertion

  • Seo, Young-Jun;Song, Geun-Sung;Cho, Won-Ho;Choi, Byung-Kwan;Cha, Seung-Heon;Baek, Sun-Yong
    • Journal of Korean Neurosurgical Society
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    • v.39 no.5
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    • pp.360-365
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    • 2006
  • Objective : This is a cadaver study to assess the accuracy of three cervical screw insertion techniques; the blind technique [Group I], the laminotomy technique [Group II], and the funnel technique [Group III]. Methods : Ten human cadavers embalmed with formaldehyde were prepared. After exposing the spinous processes, the laminas and the lateral masses, titanium alloy transpedicular screws were inserted from C3 to C7. A total of 100 pedicles were ramdomly assigned to one of three techniques [the blind technique : 31 screws, the laminotomy technique : 51 screws, the funnel technique: 18 screws]. Axial computed tomography with 1-mm slices, and sagittal and coronal reformation were performed to identify the accuracy of the screw insertion and the anatomic relationships. Results : In Group I, 9 screws [29%] were either contained within or penetrated less than 1mm, which were rated as successful. In Group II, 24 screws [47%] were successful. In Group III, 16 screws [89%] were successful. In the multiple comparison, there was a statistically significant difference between Groups I and III and between Groups II and III [$X^2$ test and Bonfenoni test]. Conclusion : The funnel technique can help a surgeon's understanding about the cervical pedicle more precisely than the other two techniques. The funnel technique is less dependent on lateral soft tissue retraction state.

Morphometric Study of the Anterior Thalamoperforating Arteries

  • Kim, Sung-Ho;Yeo, Dong-Kyu;Shim, Jae-Joon;Yoon, Seok-Mann;Chang, Jae-Chil;Bae, Hack-Gun
    • Journal of Korean Neurosurgical Society
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    • v.57 no.5
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    • pp.350-358
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    • 2015
  • Objective : To evaluate the morphometry of the anterior thalamoperforating arteries (ATPA). Methods : A microanatomical study was performed in 79 specimens from 42 formalin-fixed adult cadaver brains. The origins of the ATPAs were divided into anterior, middle, and posterior segments according to the crowding pattern. The morphometry of the ATPAs, including the premammillary artery (PMA), were examined under a surgical microscope. Results : The anterior and middle segments of the ATPAs arose at mean intervals of $1.75{\pm}1.62$ mm and $5.86{\pm}2.05$ mm from the internal carotid artery (ICA), and the interval between these segments was a mean of $3.17{\pm}1.64$ mm. The posterior segment arose at a mean interval of $2.43{\pm}1.46$ mm from the posterior cerebral artery (PCA), and the interval between the middle and posterior segments was a mean of $3.45{\pm}1.39$ mm. The mean numbers of perforators were $2.66{\pm}1.19$, $3.03{\pm}1.84$, and $1.67{\pm}0.98$ in the anterior, middle, and posterior segments, respectively. The PMA originated from the middle segment in 66% of cases. A perforator-free zone was located >2 mm from the ICA in 30.4% and >2 mm from the PCA in 67.1% of cases. Conclusion : Most perforators arose from the anterior and middle segments, within the anterior two-thirds of the posterior communicating artery (PCoA). The safest perforator-free zone was located closest to the PCA. These anatomical findings may be helpful to verify safety when treating lesions around the PCoA and in the interpeduncular fossa.

Characterization of the Anatomic Location of the Pituitary Stalk and Its Relationship to the Dorsum Sellae, Tuberculum Sellae and Chiasmatic Cistern

  • Gulsen, Salih;Dinc, Ahmet Hakan;Unal, Melih;Canturk, Nergis;Altinors, Nur
    • Journal of Korean Neurosurgical Society
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    • v.47 no.3
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    • pp.169-173
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    • 2010
  • Objective : The normal anatomic relationships characteristic of the pituitary stalk area were previously thought to involve only one location. The purpose of this study was to re-evaluate the anatomic location of the pituitary stalk and possible varying locations in relation to the tuberculum sellae and dorsum sellae using morphometric evaluation and anatomic dissection of human cadaveric specimens. The surgical implications of the variations are discussed. Methods : The calvaria were removed via routine autopsy dissections, and the brains were removed from the skull while preserving the pituitary stalk. The diaphragma sellae, tuberculum sellae, and the location of the pituitary stalk were examined in 60 human cadaveric heads obtained from fresh adult cadavers. Empty sellae were excluded. Results : The openings of the diaphragma sellae averaged $6.62{\pm}1.606mm$ (range, 3-9 mm). The distance between the tuberculum sellae and the posterior part of the pituitary stalk was 1 to 8 mm. The upper face of the diaphragma sellae appeared flat in 26 (43%), concave in 24 (40%), and convex in 6 cases (10%), with a prominent tuberculum sellae in 4 cases (7%). The location of the chiasm was normal in 47 cases (78%), with a prefixed chiasm in 3 cases (5%) and a postfixed chiasm (17%) in the 10 cases. Four cadaver specimens had prominent tuberculum sellae and other parameters were not evaluated. Conclusion : When opening the chiasmatic cistern, neurosurgeons should be aware about the relationship between the pituitary stalk and the surrounding structures to prevent inadvertent injury to the pituitary stalk.

Clinically Correlated Anatomical Basis of Cricothyrotomy and Tracheostomy

  • Gulsen, Salih;Unal, Melih;Dinc, Ahmet Hakan;Altinors, Nur
    • Journal of Korean Neurosurgical Society
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    • v.47 no.3
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    • pp.174-179
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    • 2010
  • Objective : Cricothyrotomy and tracheostomy are performed by physicians in various disciplines. It is important to know the comprehensive anatomy of the laryngotracheal region. Hemorrhage, esophageal injury, recurrent laryngeal nerve injury, pneumothorax, hemothorax, false passage of the tube and tracheal stenosis after decannulation are well known complications of the cricothyrotomy and tracheostomy. Cricothyrotomy and tracheostomy should be performed without complications and as quickly as possible with regards the patients' clinical condition. Methods : A total of 40 cadaver necks were dissected in this study. The trachea and larynx and the relationship between the trachea and larynx and the surrounding structures was investigated. The tracheal cartilages and annular ligaments were counted and the relationship between tracheal cartilages and the thyroid gland and vascular structures was investigated. We performed cricothyrotomy and tracheostomy in eleven cadavers while simulating intensive care unit conditions to determine the duration of those procedures. Results : There were 11 tracheal cartilages and 10 annular ligaments between the cricoid cartilage and sternal notch. The average length of trachea between the cricoid cartilage and the suprasternal notch was 6.9 to 8.2 cm. The cricothyroid muscle and cricothyroid ligament were observed and dissected and no vital anatomic structure detected. The average length and width of the cricothyroid ligament was 8 to 12 mm and 8 to 10 mm, respectively. There was a statistically significant difference between the surgical time required for cricothyrotomy and tracheostomy (p < 0.0001). Conclusion : Tracheostomy and cricothyrotomy have a low complication rate if the person performing the procedure has thorough knowledge of the neck anatomy. The choice of tracheostomy or cricothyrotomy to establish an airway depends on the patients' clinical condition, for instance; cricothyrotomy should be preferred in patients with cervicothoracal injury or dislocation who suffer from respiratory dysfunction. Furthermore; if a patient is under risk of hypoxia or anoxia due to a difficult airway, cricothyrotomy should be preferred rather than tracheostomy.

Evaluation of the effective dose and image quality of low-dose multi-detector CT for orthodontic treatment planning (3차원 안모분석을 위한 저선량 Multi-detector CT 영상의 유효선량 및 화질 평가)

  • Chung, Gi-Chung;Han, Won-Jeong;Kim, Eun-Kyung
    • Imaging Science in Dentistry
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    • v.40 no.1
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    • pp.15-23
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    • 2010
  • Purpose : This study was designed to compare the effective doses from low-dose and standard-dose multi-detector CT (MDCT) scanning protocols and evaluate the image quality and the spatial resolution of the low-dose MDCT protocols for clinical use. Materials and Methods : 6-channel MDCT scanner (Siemens Medical System, Forschheim, Germany), was used for this study. Protocol of the standard-dose MDCT for the orthodontic analysis was 130 kV, 35 mAs, 1.25 mm slice width, 0.8 pitch. Those of the low-dose MDCT for orthodontic analysis and orthodontic surgery were 110 kV, 30 mAs, 1.25 mm slice width, 0.85 pitch and 110 kV, 45 mAs, 2.5 mm slice width, 0.85 pitch. Thermoluminescent dosimeters (TLDs) were placed at 31 sites throughout the levels of adult female ART head and neck phantom. Effective doses were calculated according to ICRP 1990 and 2007 recommendations. A formalin-fixed cadaver and AAPM CT performance phantom were scanned for the evaluation of subjective image quality and spatial resolution. Results : Effective doses in ${\mu}Sv$ ($E_{2007}$) were 699.1, 429.4 and 603.1 for standard-dose CT of orthodontic treatment, low-dose CT of orthodontic analysis, and low-dose CT of orthodontic surgery, respectively. The image quality from the low-dose protocol were not worse than those from the standard-dose protocol. The spatial resolutions of both standard-dose and low-dose CT images were acceptable. Conclusion : From the above results, it can be concluded that the low-dose MDCT protocol is preferable in obtaining CT images for orthodontic analysis and orthodontic surgery.

The effect of varying peripheral bone structure and bone density on the occlusal stress distribution of human premolar regions (사람 소구치부위에서 주위골의 구조 및 밀도변화가 교합력에 의한 치아의 응력분포에 미치는 영향)

  • Suh, Ye-Joon;Shim, June-Sung;Lee, Keun-Woo;Chung, Moon-Kyu;Lee, Ho-Yong
    • Journal of Dental Rehabilitation and Applied Science
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    • v.19 no.1
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    • pp.7-15
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    • 2003
  • This study used FEM(Finite Element method) based on micro-CT images to see the effects of occlusal force distribution with varying bone density and structure. the mandibular premolar region from human cadaver, thickness of 10mm was imaged using micro-CT. the cross sectional images were taken every $10{\mu}m$. these were reconstructed and the longitudinal image at the mid point of mesiodistal of the speciman was obtained for the specimen for the FEM. The stress disribution produced by a vertical force at 100N and 100N horizontal were analyzed by MSC Nastran FEM Package. according to the result of this study the occlusal force distribution depends on the structure of cancellus bone and for further information on the occlusal force distribution on the tooth and the surrounding structure requires further studies on cancellus bone structure. CEJ of all model show the highest peak and region whice meet teeth and bone show second high peak. Original model and cortical bone add model show different stress distribution. Stress distribution changed according to bone structures and densities.

Accuracy of virtual models in the assessment of maxillary defects

  • Kamburoglu, Kivanc;Kursun, Sebnem;Kilic, Cenk;Ozen, Tuncer
    • Imaging Science in Dentistry
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    • v.45 no.1
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    • pp.23-29
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    • 2015
  • Purpose: This study aimed to assess the reliability of measurements performed on three-dimensional (3D) virtual models of maxillary defects obtained using cone-beam computed tomography (CBCT) and 3D optical scanning. Materials and Methods: Mechanical cavities simulating maxillary defects were prepared on the hard palate of nine cadavers. Images were obtained using a CBCT unit at three different fields-of-views (FOVs) and voxel sizes: 1) $60{\times}60mm$ FOV, $0.125mm^3$ ($FOV_{60}$); 2) $80{\times}80mm$ FOV, $0.160mm^3$ ($FOV_{80}$); and 3) $100{\times}100mm$ FOV, $0.250mm^3$ ($FOV_{100}$). Superimposition of the images was performed using software called VRMesh Design. Automated volume measurements were conducted, and differences between surfaces were demonstrated. Silicon impressions obtained from the defects were also scanned with a 3D optical scanner. Virtual models obtained using VRMesh Design were compared with impressions obtained by scanning silicon models. Gold standard volumes of the impression models were then compared with CBCT and 3D scanner measurements. Further, the general linear model was used, and the significance was set to p=0.05. Results: A comparison of the results obtained by the observers and methods revealed the p values to be smaller than 0.05, suggesting that the measurement variations were caused by both methods and observers along with the different cadaver specimens used. Further, the 3D scanner measurements were closer to the gold standard measurements when compared to the CBCT measurements. Conclusion: In the assessment of artificially created maxillary defects, the 3D scanner measurements were more accurate than the CBCT measurements.