• 한국동물생명공학회지
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• 제35권2호
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• pp.198-206
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• 2020

Food and agricultural production sector, especially livestock production is vital for Mongolia's economic and social development. Domestic sheep play key roles for Mongolians, providing food (meat, milk) and raw materials (wool, sheepskin), but genetic diversity, origin of sheep populations in Mongolia have not been well studied. Studies of population genetic diversity is important research field in conservation and restoration of animal breeds and genetic resources. Therefore, this study aimed to investigate genetic characteristics and estimate origin through the analysis of mitochondrial DNA control region D-loop and Cytochrome b of Mongolian indigenous sheep (Mongolian native, Orkhon and Altanbulag) and one Europe sheep (Suffolk). As a result of there were found, 220 SNPs (Single nucleotide polymorphism) in the D-loop region, 28 SNPs in the Cytochrome B region, furthermore, 77 Haplotypes. The nucleotide diversity was only found in D-loop region (n = 0.0184). Phylogenetic analysis showed that 3 (A, B, and C) of 5 haplogroups of sheep have been identified in our research. Haplogroup C was only found in Mongolian indigenous sheep. Haplogroup D and E were not observed. As a result of haplogroups, haplogroup A was dominant (n = 46 of 94 sheeps), followed by haplogroup B (n = 36) and haplogroup C (n = 12). Sequence analysis showed that T deletion, insertion and heteroplasmy in D-loop region occurred at a high rate in Mongolian indigenous sheep population (T insertion = 47, T deletion = 83). The heteroplasmy, which has never been found in Mongolian sheep, has been newly discovered in this study. As a result, the Mongolian sheep varieties, which mainly derived from Asia, were in hybridization with European sheep varieties.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.84-84
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• 2017

Eleutherococcus senticosus (Siberian ginseng) is an important medicinal tree found in Russian taiga, and northern regions of Korea, Japan, and China. In this study, we analyzed the genome-wide distribution of microsatellites in E. senticosus using developed SSR markers. A total of 711 clones from an SSR-enriched genomic DNA library were sequenced, of which 47 clones (6.6%) were redundant. Of the 664 independent clones, only 12 polymorphic SSR markers were obtained, which also revealed successful amplicons in E. senticosus accessions. Using the developed SSR markers, we estimated genetic diversity and population structure among 131 E. senticosus accessions in Korea and China. The number of alleles ranged from 2 to 11, with an average of 7.4 alleles. The mean values of observed heterozygosity ($H_O$) and expected heterozygosity ($H_E$) were 0.59 and 0.56, respectively. The average polymorphism information content (PIC) was 0.51 in all 131 E. senticosus accessions. E. senticosus accessions in Korea and China showed a close genetic similarity. Significantly low pairwise genetic divergence was observed between the two regions, suggesting a relatively narrow level of genetic basis among E. senticosus accessions. Our results not only provide molecular tools for genetic studies in E. senticosus but are also helpful for conservation and E. senticosus breeding programs.

• The Plant Pathology Journal
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• 제29권4호
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• pp.397-409
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• 2013

The full-genome sequences of fourteen isolates of Broad bean wilt virus 2 (BBWV2), collected from broad bean, pea, spinach, bell pepper and paprika plants in Korea during the years 2006-2012, were determined and analyzed comparatively along with fifteen previously reported BBWV2 genome sequences. Sequence analyses showed that RNA-1 and RNA-2 sequences of BBWV2 Korean isolates consisted of 5950-5956 and 3568-3604 nucleotides, respectively. Full-length genome sequence-based phylogenetic analyses revealed that the BBWV2 Korean isolates could be divided into three major groups comprising GS-I (isolates BB2 and RP7) along with isolate IP, GS-II (isolates BB5, P2, P3 and RP3) along with isolate B935, and GS-III including 16 BBWV2 Korean isolates. Interestingly, GS-III appears to be newly emerged and predominant in Korea. Recombination analyses identified two recombination events in the analyzed BBWV2 population: one in the RNA-1 of isolate K and another one in the RNA-2 of isolate XJ14-3. However, no recombination events were detected in the other 21 Korean isolates. On the other hand, out of 29 BBWV2 isolates, 16 isolates were found to be re-assortants, of which each RNA segment (i.e. RNA1 and RNA2) was originated from different parental isolates. Our findings suggested that reassortment rather than recombination is a major evolutionary force in the genetic diversification of BBWV population in Korea.

• Journal of the korean society of oceanography
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• 제33권1-2호
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• pp.35-40
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• 1998

In February and April 1997, three temporary interruptions of electric power production at the Uljin Nuclear Power Plant in the east coast of Korea were caused by the malfunction of the cool-ing-water supply unit. The clogging of the drum screens inside the unit caused by the surface swarm of the euphausiid Euphausia pacifica Hansen might be responsible for the malfunction. These incidents were of particular interest since such interruption of reactors' operation by krill swarms had not previously been reported. Using samples caught by the drum screens inside the cooling water-supply unit, we investigated the population structure of surface swarms. One occasion of nighttime and three occasions of daytime surface swarms were found in February and April 1997, respectively. The foreguts of more than 60% of E. pacifica in nighttime surface swarm were in full condition. This evidence suggests that E. pacifica aggregates to the surface water at night for feeding. In daytime surface swarms consisting of mature E. pacifica, however, foreguts in full condition were only found in less than 10eio of krill examined, suggesting that daytime surface swarms are closely related to breeding activity. During the study period, the growth rate of mature females was more than twice higher than that of mature males. Analyses of the sex-ratio and length-frequency data show a decrease in the portion of males with increasing size. There was a decline in the number of males of 19 mm in length. Energy loss during spermatophore transfer may result in the death of male E. pacifica, as found in male E. superba.

• Asian-Australasian Journal of Animal Sciences
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• 제20권12호
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• pp.1791-1797
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• 2007

A study was conducted with 70 Hanwoo (Korean cattle) for genotyping bovine leukocyte antigen (BoLA)-DRB3.2 gene by using the polymerase chain reaction (PCR) and sequence based typing (SBT). Two-step PCR was carried out for amplifying a 284 bp fragment of the target gene and the PCR products were digested with three restriction enzymes namely RsaI, BstYI and HaeIII. Seventeen alleles were detected with frequencies ranging from 1.43 to 18.57% and one (x'aa) of these alleles was identified as a new allele that has not been reported before. The frequency of the new x'aa allele identified in this breed was 12.86%. In addition, the seven most frequently observed alleles (DRB3.2 *10, *15, *16, *26, *27, *54 and x'aa) accounted for 74.28% of the alleles in this population. The phylogenetic tree showed that the BoLA-DRB3.2 allele sequences of Hanwoo were shared with other Bos taurus breeds and no specific clade for Hanwoo was identified. It indicates high heterogeneity of the BoLA-DRB3 gene in this population and may give some ideas for breeding animals having better disease resistance.

• Asian-Australasian Journal of Animal Sciences
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• 제27권10호
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• pp.1399-1405
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• 2014

The effective management of endangered animal genetic resources is one of the most important concerns of modern breeding. Evaluation of genetic diversity and relationship of local breeds is an important factor towards the identification of unique and valuable genetic resources. This study aimed to analyze the genetic diversity and population structure of six Korean native chicken breeds (n = 300), which were compared with three imported breeds in Korea (n = 150). For the analysis of genetic diversity, 30 microsatellite markers from FAO/ISAG recommended diversity panel or previously reported microsatellite markers were used. The number of alleles ranged from 2 to 15 per locus, with a mean of 8.13. The average observed heterozygosity within native breeds varied between 0.46 and 0.59. The overall heterozygote deficiency ($F_{IT}$) in native chicken was $0.234{\pm}0.025$. Over 30.7% of $F_{IT}$ was contributed by within-population deficiency ($F_{IS}$). Bayesian clustering analysis, using the STRUCTURE software suggested 9 clusters. This study may provide the background for future studies to identify the genetic uniqueness of the Korean native chicken breeds.

• Journal of Animal Science and Technology
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• 제47권2호
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• pp.159-166
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• 2005

Porcine myostatin(MS1N) gene plays a key role in the differentiation of myoblast and muscle development. Genetic polymorphism was screened by single stranded conformation polymorphism(SSCP) analysis and subsequent DNA sequencing detected a nucleotide substitution(C2150T) in exon 3 of MSIN gene. Phenotypic association of the polymorphism was tested in a Landrace population and positive effects of the allele T for lean growth traits were found in the population. Even though it is not significant, the pigs have IT and TC genotypes were heavier for the body weight at birth and at twenty weeks of age than those containing genotype. Cc. However, the allele T was significantly associated with higher eye muscle area(P < 0.05). As a result of this study, we suggested that the allele T in exon 3 of MSTN gene comes a significant effect for increasing the eye muscle area without decreasing backfat thickness. This polymorphism did not change the amino acid but Taq I -RFLP matched to SSCP band patterns in exon 3 of MSTN gene, which will be an useful molecular marker for breeding of Landrace pigs.

• Journal of Plant Biotechnology
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• 제44권3호
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• pp.312-319
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• 2017

The need to preserve and use plant genetic resources is widely recognized, and the prospect of dwindling plant genetic diversity, coupled with increased demands on these resources, has made them a topic of global discussion. In the present study, the genetic diversity and population structure of 73 ginseng accessions collected from six regions in China were analyzed using eight simple sequence repeat (SSR) markers. Major allele frequencies ranged between 0.38 ~ 0.78, with a mean allele frequency value of 0.571. The number of alleles discovered ranged from 3 to 10 per accession, with a mean number of 7; 56 alleles were discovered in total. Gene diversity (GD) and polymorphic information content (PIC) values were similar to each other, and they ranged from 0.36 ~ 0.77 (mean 0.588) and 0.33 ~ 0.74 (mean 0.548), respectively. Accessions were divided into three clusters based on their phylogenetic relationships and genetic similarities, and although the populations were similar, they were not classified according to the region. Regional genetic diversity was also similar, with slight differences observed based on the number of accessions per region. It is expected that the findings of the present study can provide basic data for future studies on ginseng genetic diversity and for breeding ginseng cultivars.

• 환경생물
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• 제32권3호
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• pp.263-269
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• 2014

농생태계의 뽕나무 경작지에서 서식하고 있는 지렁이상을 확인하기 위하여, 2013년 4월부터 2014년 3월까지 조사를 하였다. 분류된 남방계지렁이는 총 5속 9종으로 다음과 같다. Aporrectodea caliginosa (Savigny, 1826), Bimastos parvus (Eisen, 1874), Eisenia fetida (Savigny, 1826), Drawida japonica (Michaelsen, 1892), Amynthas heteropodus (Goto & Hatai, 1898), Amynthas agrestis (Goto & Hatai, 1899), Amynthas corticis (Kinberg, 1867), Amynthas hilgendorfi (Michaelsen, 1895), Amynthas sp. 등이다. 장미줄지렁이 (Ap. caliginosa) 개체군 밀도 소장은 10월에 정점을 보이고 다음해 2월까지 감소하고, 성체와 미성체는 동시에 출현하며, 그들의 조성 비율은 성체 11.4%, 준성체 12.6 11%, 미성체 76% 이었다.

• 한국동물학회지
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• 제10권2호
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• pp.18-22
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• 1967

The routine entomological investigations were carried out in the areas of Kaejeon-Myon, OkkuGun, Cholla Pukdo province in 1964 and Sinchang-Myon, Asan Gun, Chungchong Namdo province in 1965, for the better understanding of the behavior of the anpheline mosquitoes especially of the population density , resting , feedign, and breeding habits. The results are as follows : 1. During the investigation , three species of anopheline mosquitoes were recorded ; Anopheles sinensis Wiedmann 1825, A, sineroides Yamada 1935, and A. yatsushiroensis Miyazaki 1951. A . yatsushiroensis Myiyasaki 1951, so far only recorded in Japan, was recorded for the first time in Korea in 1964 ; the site was Kaejong Myon, Okku Gun, Cholla Pukdo province. 2. Anopheles mosquitoes begin to appear from the middle of April and disappear in October. The date of mosquitoes collected by resting place collection in cow shed are three weeks ahead to the night time cow biting collection. 3. Resting places of anopheline mosquitoes are mainly in cow shed and outdoors which provides high humidity and shadow. 4. The population density of a. sinensis sows a peak in late June and early July in cow shed and by cow biting collection respectively, and another small peak in late August and early September. 5, . the biting activity at night is throughout the night from dusk to dawn, sharply decreasing just before dawn. The peak period was different in each months, 2100-2200 hours in June, 2300-2400 in July , 0300-0400 in August , and 2300-2400 in September. 6. The minimum temperature required for the mosquito's biting activity is 15 $^{\circ}C$ and the optimum is between 24-$25^{\circ}C$ : over $25^{\circ}C$ the activity is decreased. 7.A , sinensis appeared to be zoophilic in Sinchang area but 13 times anthrophophilic in Kaejong area than the former. 8. the light attraction of a. sinensis is significantly low in middle June and early August. The parous rate of A. sinensis caught by cow biting collection appeared higher after midnight (2400-0300 hours) with no relation to the peak period of biting activity by month.