The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Molecular Characterization and Variation of the Broad bean wilt virus 2 Isolates Based on Analyses of Complete Genome Sequences

  • Kwak, Hae-Ryun (Crop Protection Division, National Academy of Agricultural Science) ;
  • Kim, Mi-Kyeong (Crop Protection Division, National Academy of Agricultural Science) ;
  • Lee, Ye-Ji (Crop Protection Division, National Academy of Agricultural Science) ;
  • Seo, Jang-Kyun (Crop Protection Division, National Academy of Agricultural Science) ;
  • Kim, Jeong-Soo (Crop Protection Division, National Academy of Agricultural Science) ;
  • Kim, Kook-Hyung (Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University) ;
  • Cha, Byeongjin (Department of Plant Medicine, Chungbuk National University) ;
  • Choi, Hong-Soo (Crop Protection Division, National Academy of Agricultural Science)
  • Received : 2013.03.27
  • Accepted : 2013.05.26
  • Published : 2013.12.01
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Abstract

The full-genome sequences of fourteen isolates of Broad bean wilt virus 2 (BBWV2), collected from broad bean, pea, spinach, bell pepper and paprika plants in Korea during the years 2006-2012, were determined and analyzed comparatively along with fifteen previously reported BBWV2 genome sequences. Sequence analyses showed that RNA-1 and RNA-2 sequences of BBWV2 Korean isolates consisted of 5950-5956 and 3568-3604 nucleotides, respectively. Full-length genome sequence-based phylogenetic analyses revealed that the BBWV2 Korean isolates could be divided into three major groups comprising GS-I (isolates BB2 and RP7) along with isolate IP, GS-II (isolates BB5, P2, P3 and RP3) along with isolate B935, and GS-III including 16 BBWV2 Korean isolates. Interestingly, GS-III appears to be newly emerged and predominant in Korea. Recombination analyses identified two recombination events in the analyzed BBWV2 population: one in the RNA-1 of isolate K and another one in the RNA-2 of isolate XJ14-3. However, no recombination events were detected in the other 21 Korean isolates. On the other hand, out of 29 BBWV2 isolates, 16 isolates were found to be re-assortants, of which each RNA segment (i.e. RNA1 and RNA2) was originated from different parental isolates. Our findings suggested that reassortment rather than recombination is a major evolutionary force in the genetic diversification of BBWV population in Korea.

Keywords

References

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