• Journal of the Korean Society of Costume
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• v.53 no.5
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• pp.111-124
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• 2003

The purpose of this study is to analyze how the women´s fashion with the design elements(silhouette, material, color, detail and trimming) of androgynous image which has appeared on our specialized fashion magazine from 1986 through 2000. As a result of this study I drew the following conclusion. 1. The bon silhouette led the market in the first stage of mid 1980. The wool and leather with the masculine image, cotton and soft material with unisex image were used to express the androgynous image. The dark and mono tone with the masculine image were used a lot in color. In women´s fashion the minimalism style with simple image was expressed escaping from the decoration, in which the detail and trimming was not found as in men´s wear. 2. It showed that more and more feminine image was accepted in the second stage of early 1990. Women´s wear silhouette with the androgynous image has changed from masculine box silhouette boomed in the first stage to hourglass silhouette and slim silhouette. The wool and leather were used a lot as the materials in the first stage but the usage of the cotton with unisex image and soft texture were getting increased. The main colors were the black and the blue but the usage of the white and the red were on the increase showing the range of the use was extending. In the meantime, the detail and trimming were hardly used like in the first stage. 3. In the third stage of the late 1990, the silhouette has changed to slim style. The wool and leather were mainly used as the materials but the cotton and silk were on the increase showing that the materials of the feminine image were used in the women´s fashion with the androgynous image as well as the materials of masculine image. In color, the black and the white of the masculine image were used increasingly by the effect of the minimalism and the usage of the colors were various for the rising frequency of the trend colors usage.

• Journal of the Korean Society of Costume
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• v.67 no.4
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• pp.1-20
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• 2017

Centered on Kathleen Kye, a designer who conveyed messages on social issues through her dresses, and established her own identity, this study aimed to examine what theme, method of expression and inner meaning her 'statement' had, as a way of expressing a critical awareness. After reviewing discussions by Duggan G. G. as well as dictionary definitions, statement fashion design can be defined as a work or fashion shows containing a message of a designer who is free from a particular trend or consumerism in various social issues. Statement fashion designers give opposing messages on social issues through their fashion collections. As a result, research shows that contemporary statement fashion designers are expressing themes of a fashion systems, fetishism, body image, collision, environment, as well as the socially disadvantaged by appropriation, reuse, slogan, metaphorical pattern and performance. Satire, awakening, challenge and support can be derived from the inner meaning of contemporary statement fashion design. In terms of the theme, method of expression and inner meaning, this study showed that KYE collection of the designer Kathleen Kye expresses critical awareness on the modern society. Research findings reveal that KYE collection include the following themes: long-term youth unemployment, conflict collision in war or on the Internet, fetishism by youth in a get-rich-quick fever, environmental issue causing destruction of an ecosystem by decrease in bee population, school violence and the socially disadvantaged related to alienated immigrants. Also, as a method of expression themes, such as metaphorical patterns, were used. The patterns used images including a skeleton, gun, heart, rope, plaster, homeless box, bee, honeycomb, chain and a slot machine. On the other hand, the inner meaning of statement in KYE collection showed satire on social issues, awakening on social issues unrecognized by the masses, and support for the socially disadvantaged.

• Journal of Oriental Neuropsychiatry
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• v.6 no.1
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• pp.61-70
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• 1995

This study was aimed to evaluate the anti-stress effect of Chungkansoyosan on the rats stressed by immobilizaton. The experimintal animals were immobilized in stress box $(5cm{\times}5cm{\times}20cm)$ for 12 hours in a day during 3 days, and administered 90ng/100g Chungkansouosan extract for 15 days before stress. The epinephrine, dopamine contents in rat brain were measured by using HPLC method. The following results were obtained: 1. Total cholesterol contents of control group was $58.1{\pm}2.56mg/dl$, that of sample group was $61.6{\pm}3.70mg/dl$. This shows decreasing effect, but not significant. 2. Triglyceride contents of control group was $39.8{\pm}6.75mg/dl$ that of sample group was $27.7{\pm}4.23mg/dl$. This shows significant decrease in sample group comparing to control group. 3.Epinephrine contents of control group was $8.21{\pm}0.31ng/mg$ that of sample group was $6.33{\pm}1.02ng/mg$ this shows significant decrease in sample group comparing to control group. 4. Dopamine contents of control group was $2.45{\pm}0.23ng/mg$ that of sample group was $1.65{\pm}0.41ng/mg$ this shows decreasing effect, but not significant. 5. The quantity of muscle relaxation by Rota Rod was improved significantly by Chungkcansoyosan in immobilizaton-stressed rats. According to the above results, it is concluded that Chunkansoyosan will be useful as a preventive and remedy against stress disease.

• Journal of Plant Biotechnology
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• v.5 no.4
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• pp.215-219
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• 2003

Optimal shoot regeneration and transformation conditions of root type chicory (Cichorium intybus L. var. sativus cv Cesare) were studied. Leaf explants were co-cultured with Agrobacterium tumefaciens, which contained NPTII as a selectable marker and a rice homeotic gene, OsMADS1, that encodes a MADS-domain-containing transcription factor. After one day of co-cultivation, explants were transferred to selection media consisting of MS basal medium supplemented with 0.5 mg/L BAP, 0.1 mg/L IAA, 70 mg/L kanamycin, and 250 mg/L cefotaxime. PCR and Southern blot analyses revealed stable integration of the OsMADS1 gene in the chicory genome. Four-teen original transgenic plants ($T_o$ plants) were acclimatized in the greenhouse and examined for their morphological characters. Most of the transgenic plants showed altered morphologies, such as short, bushy, and early-flowering phenotypes with reduced apical dominance. Additionally, half of the transgenic plants exhibited altered leaf shapes, and 4 out of 14 plants were sterile. These phenotypes were inherited by the next generation. Northern blot analysis confirmed expression of the OsMADS1 gene in both floral and vegetative organs.

• ALGAE
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• v.33 no.2
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• pp.143-156
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• 2018

Dolichospermum is a filamentous and heterocytous cyanobacterium that is one of the commonly occurring phytoplanktons in the Han River of Korea. Morphological observations led to the identification of D. planctonicum-like filaments in seasonal water samples. In the present study, we successfully isolated these filaments using culture methods, and examined its morphology using light and scanning electron microscopy. The morphology of the D. planctonicumlike species differed from that of typical D. planctonicum; it had thin cylindrical-shaped akinetes, which were narrower towards the ends than at the center. This morphology is firstly described in the genus Dolichospermum. In addition, the akinetes in the filament developed solitarily and were distant from the heterocytes. Phylogenetic analysis of the 16S rRNA sequences showed that our Dolichospermum clustered with D. planctonicum and D. circinale, which have coiled trichome. However, phylogenetic analysis of the gene encoding rivulose-1,5-bisphosphate carboxylase (rbcLX) clearly separated our species from other Dolichospermum, forming a unique clade. Additionally, structures of D. planctonicum and D. hangangense strains were different type in Box-B and V3 region. These results demonstrated that the new Dolichospermum species was unique in morphology and molecular traits. Therefore, we propose this to be a new species belonging to genus Dolichospermum with the name Dolichospermum hangangense sp. nov.

• Journal of Advanced Marine Engineering and Technology
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• v.31 no.1
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• pp.26-33
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• 2007

The intake swirl motion, as one of dominant effects for an engine combustion. is very effective for turbulence enhancement during the compression process in the cylinder of 2-valve engine. Because the combustion flame speed is determined by the turbulence that is mainly generated from the mean flow of the charge air motion in intake port system. This paper describes the experimental results of swirl flow and combustion characteristics by using the oil spot method and back-scattering Laser Doppler velocimeter (LDV) in 2-valve single cylinder transparent LPG engine using the liquid phase LPG injection. For this. various intake port configurations were developed by using the flow box system and swirl ratios for different intake port configurations were determined by impulse swirl meter in a steady flow rig test. And the effects of intake swirl ratio on combustion characteristics in an LPG engine were analyzed with some analysis parameters that is swirl ratio. mean flow coefficient, swirl mean velocity fuel conversion efficiency. combustion duration and cyclic variations of indicated mean effective pressure(IMEP). As these research results, we found that the intake port configuration with swirl ratio of 2.0 that has a reasonable lean combustion stability is very suitable to an $11{\ell}$ heavy-duty LPG engine with liquid phase fuel injection system. It also has a better mean flow coefficient of 0.34 to develope a stable flame kernel and to produce high performance. This research expects to clarify major factor that effects on the design of intake port efficiently with the optimized swirl ratio for the heavy duty LPG engine.

• Natural Product Sciences
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• v.24 no.2
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• pp.103-108
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• 2018

The usage of wild ginseng (Panax ginseng C.A. Meyer) has been limited due to short supply and high price. Therefore, sufficient production as well as efficient extraction of mountain ginseng are required for the development as products. In this study, wild ginseng adventitious root cultures were prepared for efficient production with advantages of fast growth and stable production. Treatment of methyl jasmonate (MJ) to wild ginseng adventitious root cultures increased the extraction yield and antioxidative activity. Further investigation on effect of extraction conditions suggested the importance of ethanol concentration on antioxidative activity and extraction yield of MJ-treated wild ginseng adventitious root cultures. Optimized extraction condition of MJ-treated wild ginseng adventitious root cultures for maximum extraction yield and antioxidative activity was determined using response surface methodology with three-level-three-factor Box-Behnken design (BBD). Extraction of 1 g MJ-treated wild ginseng adventitious root culture with 30 ml of 9% ethanol at $30^{\circ}C$ produced 310.2 mg extract with 71.0% antioxidative activity at $100{\mu}g/ml$. Taken together, MJ-treated wild ginseng adventitious root culture is valuable source for wild ginseng usage and optimized extraction condition can be used for the development of functional products or folk remedies.

• The Plant Pathology Journal
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• v.32 no.3
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• pp.228-241
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• 2016

In our previous study, we reported that a novel endophytic bacterium Bacillus oryzicola YC7007 has suppressed bacterial diseases of rice via induced systemic resistance and antibiotic production. This endophytic strain, B. oryzicola YC7007 was used as a biological control agent against bakanae disease of rice caused by Fusarium fujikuroi, and its mechanism of interaction with the pathogen and the rice was further elucidated. Root drenching with B. oryzicola YC7007 suspension reduced the disease severity of bakanae significantly when compared with the untreated controls. The treatments of B. oryzicola YC7007 suspension ($2.0{\times}10^7cfu/ml$) to the rice rhizosphere reduced bakanae severity by 46-78% in pots and nursery box tests containing autoclaved and non-autoclaved soils. Moreover, in the detached rice leaves bioassay, the development of necrotic lesion and mycelial expansion of F. fujikuroi were inhibited significantly by spraying the culture filtrate of B. oryzicola YC7007. Drenching of ethyl acetate extracts of the culture filtrate to the rhizosphere of rice seedlings also reduced the bakanae disease severity in the plant culture dish tests. With the root drenching of B. oryzicola YC7007 suspension, the accumulation of hydrogen peroxide was observed at an early stage of rice seedlings, and a hormonal defense was elicited with and without pathogen inoculation. Our results showed that the strain B. oryzicola YC7007 had a good biocontrol activity against the bakanae disease of rice by direct inhibition, and was also capable of inducing systemic resistance against the pathogen via primed induction of the jasmonic acid pathway.

• Research in Plant Disease
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• v.14 no.2
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• pp.127-130
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• 2008

Rhizopus soft rot caused by Rhizopus stolonifer on June, 2008 occurred on Rubus crataegifolius Bunge in the box at Jinju City Wholesale Market of Agricultural Products at Gyeongnam province in Korea. The infected fruits were rapidly water-soaked, softened, black and eventually rotted. The symptoms occurred after infecting wounds at harvest time. The colony were white to light brown, formed much sporangiospores. The optimum temperature of the fungus on potato dextrose agar was $30^{\circ}C$. Sporangiophores were $12{\sim}25{\mu}m$ in width. Sporangia were globose or hemispheric, white at first and gradually to black, and $82{\sim}195{\mu}m$ in size. Columella were hemispheric, and $70{\sim}92{\mu}m$ in size. Sporangiospores were irregular round or oval, brownishblack and $9{\sim}21{\times}7{\sim}8{\mu}m$ in size. On the basis of symptom, mycological characteristics and pathogenicity of the fungus, the causal fungus was identified as Rhizopus stolonifer. This is the first report of Rhizopus soft rot by R. stolonifer on R. crataegifolius in Korea.

• Research in Plant Disease
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• v.17 no.2
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• pp.111-120
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• 2011

A bacterial artificial chromosome library of Pectobacterium carotovorum 35 was constructed to characterize the genome and to sequence its hrp region. The hrp cluster of P. carotovorum 35 consisted of 26 open reading frames in five operons. A promoter-based green fluorescent protein technology was used to identify the genes regulated by the alternative sigma factor, HrpL, in P. carotovorum 35. The majority of the selected clones contained the hrpJ operon promoter sequence, which harbors a hrp box, but no putative hrp boxes were detected within the promoter sequences of two other hrpL-regulated genes encoding for pectate lyase and large repetitive protein. Although the promoters of five other hrp operons also contained hrp boxes, their expression was not HrpL-dependent in the promoter-based selection in E. coli. However, transcriptional analysis showed that expression from all operons harboring hrp boxes, except for the hrpN operon, was reduced significantly in the hrpL mutant. The severity of soft-rot symptoms when the hrpL mutant was applied to the surface of tobacco leaves, mimicking natural infection, was greatly attenuated. These results indicate that the hrpL gene of P. carotovorum 35 may be involved in the development of soft-rot symptoms.