Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Alternative Sigma Factor HrpL of Pectobacterium carotovorum 35 is Important for the Development of Soft-rot Symptoms

  • Nam, Hyo-Song (Bio Control Center, Jeonnam Bioindustry Foundation) ;
  • Park, Ju-Yeon (Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Kang, Beom-Ryong (Jeonnam Agricultural Extension Service Center) ;
  • Lee, Sung-Hee (Chungbuk Agricultural Research and Extension Services) ;
  • Cha, Jae-Soon (Department of Agricultural Medicine, Choongbuk National University) ;
  • Kim, Young-Cheol (Institute of Environmentally-Friendly Agriculture, Chonnam National University)
  • Received : 2011.05.31
  • Accepted : 2011.07.16
  • Published : 2011.08.31
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Abstract

A bacterial artificial chromosome library of Pectobacterium carotovorum 35 was constructed to characterize the genome and to sequence its hrp region. The hrp cluster of P. carotovorum 35 consisted of 26 open reading frames in five operons. A promoter-based green fluorescent protein technology was used to identify the genes regulated by the alternative sigma factor, HrpL, in P. carotovorum 35. The majority of the selected clones contained the hrpJ operon promoter sequence, which harbors a hrp box, but no putative hrp boxes were detected within the promoter sequences of two other hrpL-regulated genes encoding for pectate lyase and large repetitive protein. Although the promoters of five other hrp operons also contained hrp boxes, their expression was not HrpL-dependent in the promoter-based selection in E. coli. However, transcriptional analysis showed that expression from all operons harboring hrp boxes, except for the hrpN operon, was reduced significantly in the hrpL mutant. The severity of soft-rot symptoms when the hrpL mutant was applied to the surface of tobacco leaves, mimicking natural infection, was greatly attenuated. These results indicate that the hrpL gene of P. carotovorum 35 may be involved in the development of soft-rot symptoms.

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References

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