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The Korean Society of Phycology (한국조류학회(藻類))
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Morphological characterization and molecular phylogenetic analysis of Dolichospermum hangangense (Nostocales, Cyanobacteria) sp. nov. from Han River, Korea

  • Choi, Hye Jeong (Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Joo, Jae-Hyoung (Research Institute for Natural Sciences, Hanyang University) ;
  • Kim, Joo-Hwan (Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Wang, Pengbin (Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Ki, Jang-Seu (Department of Biotechnology, Sangmyung University) ;
  • Han, Myung-Soo (Department of Life Science, College of Natural Sciences, Hanyang University)
  • Received : 2018.02.21
  • Accepted : 2018.05.02
  • Published : 2018.06.15
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Abstract

Dolichospermum is a filamentous and heterocytous cyanobacterium that is one of the commonly occurring phytoplanktons in the Han River of Korea. Morphological observations led to the identification of D. planctonicum-like filaments in seasonal water samples. In the present study, we successfully isolated these filaments using culture methods, and examined its morphology using light and scanning electron microscopy. The morphology of the D. planctonicumlike species differed from that of typical D. planctonicum; it had thin cylindrical-shaped akinetes, which were narrower towards the ends than at the center. This morphology is firstly described in the genus Dolichospermum. In addition, the akinetes in the filament developed solitarily and were distant from the heterocytes. Phylogenetic analysis of the 16S rRNA sequences showed that our Dolichospermum clustered with D. planctonicum and D. circinale, which have coiled trichome. However, phylogenetic analysis of the gene encoding rivulose-1,5-bisphosphate carboxylase (rbcLX) clearly separated our species from other Dolichospermum, forming a unique clade. Additionally, structures of D. planctonicum and D. hangangense strains were different type in Box-B and V3 region. These results demonstrated that the new Dolichospermum species was unique in morphology and molecular traits. Therefore, we propose this to be a new species belonging to genus Dolichospermum with the name Dolichospermum hangangense sp. nov.

Keywords

References

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