• The Korea Journal of Herbology
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• v.32 no.4
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• pp.53-60
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• 2017

Objectives : Collagen decrease of Skin appears through various path ways. One of causes may be the Advanced glycation endproducts (AGEs) that combine formation of glucose and protein. The aim of this study was to explore the prevent wrinkle formation of Paeoniae radix (PR) and heated Paeoniae radix (HPR) via AGEs path way. Methods : AGEs formation inhibitory activities of PR and HPR measured using bovine serum albumin, glucose, and fructose. To evaluate the protective effects of PR and HPR in diabetic rats induced with streptozotocin (STZ) and methyl glyoxal (MGO), SD rat were distributed into four groups. Normal rats (Nor), AGEs-induced rats (Con), AGEs-induced rats treated with 100 mg/kg PR(PR), AGEs-induced rats treated with 100 mg/kg HPR (HPR). To induce AGEs, streptozotocin (50 mg/kg) was administered intraperitoneally and after 3 days administrated 100mM methyl glyoxal for 3 weeks. Results : The oral administration of HPR inhibited AGEs in skin tissues compared with PR. The increased reactive oxygen species (ROS) levels in the serum were diminished by HPR treatment. The analyses of kidney and skin tissues proteins indicated that HPR treatment effectively reduced AGEs related protein levels as compared to that by PR treatment. Also, HPR decreased anti-oxidant related protein levels in skin tissues such as catalase, glutathione peroxidase. Moreover, it inhibited the reduction of COL1A2 by decreasing MMP-1. Conclusion : Based on these results, it was suggested that PR and HPR could have Improving effects on wrinkle formation. These evidences provide useful information for the development wrinkle formation treated agent.

• The Korea Journal of Herbology
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• v.32 no.4
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• pp.17-24
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• 2017

Objectives : Advanced glycation end products (AGEs) is bind formation of glucose and protein. Acceleration of AGE formation during hyperglycemia is associated with the pathogenesis of diabetic complications and causes kidney and skin damage. The aim of this study was investigated the AGEs inhibitory activity and antioxidant activity of water extracts from young persimmon (YP) and heated young persimmon (HYP). Methods : Paeoniae Radix Alba (YP) is prepared by heating with 30% ethanol. AGEs formation inhibitory activities of YP and HYP measured using bovine serum albumin. To evaluate the protective effects of YP and HYP in diabetic rats induced with streptozotocin (STZ) and methyl glyoxal (MGO), SD rats were distributed into four groups; normal mice (Nor), AGEs-induced rats (Con), AGEs-induced rats treated with 100 mg/kg YP (YP), AGEs-induced rats treated with 100 mg/kg heated YP (HYP) for 3weeks. Heated young persimmon respectively decrease AGEs construction. Results : YP and HYP administration inhibited the biomarkers of AGEs in serum, kidney and skin tissues. AGE-induced rats revealed that the significant decreased collagen however, heat processing methods of young persimmon up regulated inhibits AGEs-induced collagen decrease. The expressions of AGEs were decreased in YP and HYP treated group compared with the control group in tissues. It specifies that HYP has potential to serve as a positive regulator of via AGEs path way. Conclusion : It has proposed that may have an improvement effect on diabetic complications, heated young persimmon has AGEs inhibitory excellent activities and antioxidant effect.

• The Korea Journal of Herbology
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• v.34 no.1
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• pp.117-124
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• 2019

Objective : Chronic acid reflux esophagitis (CARE), one of gastroesophageal reflux disease (GERD) is increasing worldwide. Coptidis rhizoma extract (CRE) is a traditional herb that cures a variety of diseases. This study was conducted to evaluate the protective effect of CR on rats with chronic acid reflux esophagitis. Methods : The antioxidant activities were evaluated through radical scavenging assays using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) radical scavenging assays. CARE was surgically induced in 5-week-old male SD rats by ligating the border between forestomach and glandular portion with a 2-0 silk tie and covering the duodenum using 18-Fr $N{\acute{e}}laton$ catheter. To evaluate the esophageal protective effect of CRE, rats were divided into 3 groups: Nor (normal rats), Veh (chronic acid reflux esophagitis induced rats), CR (chronic acid reflux esophagitis induced rats treated with CRE 200 mg/kg body weight). Results : The administration of CRE significantly prevented the mucosal injury of the esophagus tissue and histological findings improved the esophageal lesion. It has been shown that inflammation is prevented by the increase of antioxidant-related factors (Nrf-2, HO-1, SOD, catalase, and GPx-1/2) through the antioxidant pathway of esophageal tissue. The administration of CRE reduced the increase of serum peroxynitrite ($ONOO^-$) and markedly reduced the protein expression of inflammatory mediator such as $NF-{\kappa}Bp65$, $p-I{\kappa}B{\alpha}$, iNOS, and IL-6. Conclusions : Overall, these results suggest that CRE administration confirmed the protective effect of esophageal mucosa, suggesting that it is a potential treatment for chronic acid reflux esophagitis.

• Journal of Life Science
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• v.32 no.4
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• pp.303-309
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• 2022

Dystaenia takesimana is an endemic plant found only in Korea, especially on Ulleung Island. The leaves and roots of D. takesimana have been used as food, forage, and oriental medicine. Anti-bacterial, anti-inflammation, antioxidant, and α-glucosidase inhibition biological activities have been reported in the plant's root extract. However, studies concerning the anti-thrombosis activities of D. takesimana are still in the rudimentary stage. In this study, the extracts of the leaf (DT-L), stem (DT-S), and root (DT-R) of D. takesimana were prepared using 70% ethanol, and their anti-thrombosis activities were evaluated. DT-L extracts (0.25 mg/ml) showed strong inhibitions against platelet aggregation, comparable to aspirin, with strong radical scavenging activities. Furthermore, the DT-L extract did not show any RBC hemolysis up to 1 m/ml. The ant-coagulation and antioxidant activities of the DT-S extract were ignorable. While the DT-R extract showed inhibitions against thrombin and blood coagulation factors, it also showed strong platelet aggregation. This is a first report of the anti-thrombosis activities of D. takesimana, and our results suggest that DT-L could be developed as a valuable bioresource for high value-added products.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.2
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• pp.27-33
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• 2019

The purpose of this study was to compare the storage and post-storage changes of Cheorwon onions and onions for each region. Onion is a perennial plant belonging to the lily family. It is native to Persia of Southwest Asia. It is cultivated much in the temperate regions of the world. Cheorwon area is inland, but it has high continental climate due to its high altitude. Therefore it is said that the onion cultivated in this region has higher sugar content and higher taste than onion grown in southern region. Although the results of onion storage experiments in Cheorwon, Muan, Buan, and Changnyeong regions did not show much difference in weight, width, and height measurements before and after storage, Brix was reduced by approximately 1-2 brix. Before and after storing in each region, Brix was the highest in Cheorwon, Changnyeong, Buan, and Muan, and after saving, onions in Cheorwon were the same order. After each region was stored, the results showed that Cheorwon (112.3g), Muan (106g), Buan (102.1g) and Changnyeong (92.6g) had the highest hardness. Cold storage tests show that onions contain the least amount of corruption in paper boxes, while Cheorwon onions have the lowest level of corruption in other areas.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.12 no.2
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• pp.61-72
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• 2007

The relationship between North Pacific chum salmon (Oncorhynchus keta) population and climate variability was investigated in the North Pacific ecosystem. Time-series for the Aleutian Low Pressure, Southern Oscillation, Arctic Oscillation, and Pacific Decadal Oscillation (PDO) indices dating back to 1950 are compared with the chum salmon catch using a cross-correlation function (CCF) and cumulative sum (CuSum) of anomalies. The results of CCF and CuSum analyses indicated that there was a major change in climate during the mid 1970s, and that the chum salmon population responded to this climate event with a time-lag. The PDO and chum salmon returns showed a highly significant correlation with a time-lag of 3 years, while the AOI with a time-lag of $6{\sim}7$ years. The favorable environments for fry chum salmon might cause better growth in the coastal areas, but higher growth rate during the early stage does not seem to be related to the improved return rate of spawning adults. Rather, growth in the Okhotsk Sea or the Bering Sea during immature stages has a significant correlation with return rate, which implies the size-related mortality process. The development of a local climate index is necessary to elucidate the effect of climate variability on the marine ecosystem around the Korean Peninsula.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.2
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• pp.84-90
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• 2018

Cultivation of high-quality forage crop by using fallow field during winter is required for national high feed self-sufficiency and establishment of self-supply system of high-quality forage crop. Field experiments for cultivation of high-quality winter forage crop were conducted at the paddy and upland fields in Cheonan and Anseong city with treatments of single Italian ryegrass(IRG) and IRG mixed with forage barley at the paddy field and of single forage barley and forage barley mixed with IRG at the upland field in the Mid-west plain. Several cultivation conditions such as broadcasting IRG seed under standing rice, sowing time, tillage method, drainage condition, mixed sowing with forage barley were compared to know the change of growth, yield and quality of winter forage crop. In particular, over-wintering rate and dry matter yield were decreased significantly in late-sown IRG and moisture-stressed forage barley. Yield and quality of forage crops were increased by sowing after tillage, mixed sowing of IRG with barley at the paddy field with good drainage. High yield as much as dry matter $10MT\;ha^{-1}$ with high feed value could be obtained by early sowing of feed barley mixed with IRG at the upland field. Cultivation conditions such as early sowing, sowing after tillage, drainage management are required for higher dry matter yield, quality and stable cultivation of winter forage crops in the mid-west plain of Korea.

• Journal of Bio-Environment Control
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• v.17 no.1
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• pp.26-31
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• 2008

This experiment were conducted to investigate the response of growth and yield of potato after transplanting of plug seedlings 'Superior' and 'Dejima' produced at different plug cell size (105, 162, and 288) and photoperiod (8/16, 12/12, and 16/8, day/night) for 20 days in controlled plant growth system. Growth and relative growth rate of plug seedling 'Superior' was affected by plug cell size and photoperiod at 7weeks after transplanting. Tuber weight of 'Superior' was increased as cell size and photoperiod increased. That of 'Dejima' was highest in 105 cell and different with photoperiod. At 90 days after transplanting, tuber weight ($258.9{\sim}471.9\;g/plant) of 'Superior' was high in 105 and 162 cell size and 16/8 hr photoperiod. That ($278.2{\sim}428.0\;g/plant$) of 'Dejima' was high in 105 cell size, but was not different with photoperiod. The number of tuber per plant was $2.6{\sim}6.9$ of 'Superior' and $2.2{\sim}3.6$ 'Dejima'. Tuber number per plant was not significantly different with cell size and photoperiod. The large tuber over 80 g was $32.0{\sim}50.9%$ of 'Superior' and $41.0{\sim}56.7%$ of 'Dejima'. The large tuber in 'Superior' and 'Dejima' lowered as the cell size decreased. The large tuber of 'Superior' increased as photoperiod increased, but that of 'Dejima' was not differed. As the results, the optimal plug cell size and photoperiod of potato seedling is considered to be below 162 cell and over 12 hr of photoperiod.

• Journal of Life Science
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• v.20 no.9
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• pp.1373-1377
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• 2010

Coastal sand-dune plants can survive very effectively with the help of various microbes, especially ecto- and endomycorrihizae. Penicillium citrinum KACC43900 was screened according to growth promoting activity on sand-dune plants Calystegia soldanella and Ischaemum anthephoroides. In this study, coastal sand-dune plants were treated with a fungal culture filtrate of endophytic fungus P. citrinum KACC43900 to confirm the promotion of plant growth. C. soldanella and I. anthephoroides were used as representative coastal sand-dune plants, and their shoot length, plant length, and dry weight were analyzed. The shoot lengths of control C. soldanella and I. anthephoroides were 8.60 cm and 8.12 cm, and those of samples treated with fungal culture filtrates of C. soldanella and I. anthephoroides for 20 days were 16.30 cm and 10.56 cm, respectively. The plant lengths of control C. soldanella and I. anthephoroides were 14.90 cm and 14.80 cm, and those of samples treated with fungal culture filtrates of C. soldanella and I. anthephoroides for 20 days were 24.06 cm and 17.06 cm, respectively. The dry weight of C. soldanella and I. anthephoroides treated with fungal culture filtrates of C. soldanella and I. anthephoroides for 20 days were 0.163 g and 0.032 g, respectively. It was shown that the growth of shoots in C. soldanella and I. anthephoroides was promoted, 89.54% and 29.60%, by culture filtrate of P. citrinum KACC43900.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.191-191
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• 2017

Hydroxy fatty acid (HFA) is an important industrial resource that known to be extracted from seeds of Castor or Lesquerella. However, mass production of HFA from those crops are difficult because of their behavior or life cycle. In this study, we applied HFA synthesis related gene FAH12, RcPDAT1, RcLPCAT, RcDGAT2, and RcPDCT on bioenergy crop Camelina sativa. Furthermore, we determined NaCl or cold stress tolerance changes of transgenic Camelina. RcFAH12, RcPDAT1, RcLPCAT, RcDGAT2, and RcPDCT genes were cloned into multigene expression vector which is engineered with seed specific promoter of FAE1 or Napin. Combination of HFA genes multi-expression vector constructs were divided into Set3 (RcFAH12, RcPDAT1-2, RcLPCAT), Set4 (RcFAH12, RcDGAT2, RCPDAT1-2, RcLPCAT), and Set5 (RcFAH12, RcDGAT2, RCPDAT1-2, RcLPCAT, RcPDCT). Transgenic HFA synthesis Camelina plants were generated using agrobacterium-mediated vacuum infiltration system. Results of fatty acid composition of T1 transgenic Camelina seeds analyzed by GC-MS showed 9.5, 9.0, and 13.6 % of HFA contents in Set3#6, Set4#8, and Set5#10, respectively. Therefore, seeds of T2 generation were harvest from Set5#10 which is shown highest HFA contents, and, 17.7, 8.1 and 10.5 % of HFA contents were determined in Set5#10-5, Set5#10-8, and Set#10-10, respectively. However, 7.7% of C18:2 and 22.3 % of C18:3 among unsaturated fatty acids were decreased in Set5#10-5 than WT. Meanwhile, we confirmed abiotic stress responses in T2 transgenic Camelina Set5#10-5 and Set5#10-10 under 0, 100, 150, and 200 mM NaCl or 25, 15, and $10^{\circ}C$ temperature for 5 weeks. Both Set5#10-5 and Set5#10-10 showed lower growth in height than WT in control and NaCl condition. Growth of leaf length and width were similar in WT and Set5#10-10 but lower in Set5#10-5 under NaCl stress. Number of opened flowers showed that both transgenic Camelina were lower than WT under normal condition. But, WT and Set5#10-10 showed similar opened flower number in 100 and 200 mM NaCl. In cold stress, 15 and $10^{\circ}C$ treatment for 5 weeks did not showed significant changes in between WT and both transgenic lines even they showed different growth rate in control condition. Taken together, growth and development are delayed by expression of exogenous HFA related genes in transgenic lines but relative abiotic stress sensitivity is similar with WT. In conclusion, reduced C18:2 or C18:3 fatty acid composition of seed by HFA synthesis is resulted from lack of resource supplement for development at seedling stage but it is not affect NaCl and cold stress tolerance.