• Natural Product Sciences
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• v.16 no.4
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• pp.228-232
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• 2010

Bioactivity-guided fractionation using on-line HPLC biochemical detection system on $CHCl_3$-soluble fraction of Lycoris radiata led to the isolation of deoxylycorenine (1), O-demethylhomolycorine (2), galanthamine (3), lycoramine (4), mixture of $6{\alpha}$-and $6{\beta}$-haemanthidine (5), and lycorine (6), identified by spectroscopic data and physicochemical property. Among the isolated compounds, 1, 3 and 6 showed acetylcholinesterase inhibitiory activities with $IC_{50}$ values of 18.0, 12.0 and $16.6\;{\mu}M$, respectively, in in vitro colorimetric microplate assay.

• The Plant Pathology Journal
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• v.28 no.2
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• pp.149-155
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• 2012

A field trial was conducted in 2009/2010 and 2010/2011 growing seasons at Giza Agricultural Research Station to examine correlations between some biochemical componets and powdery mildews ($PM_s$) resistance in flax cultivars. Nine flax cultivars could be divided into five distinct groups, i.e., highly susceptible (Cortland and C.I. 2008), moderately susceptible (Giza 7, and Marshall), moderately resistant (Cass), resistant (Koto, Dakota and Wilden), and highly resistant (Ottowa 770B). The cultivars showed considerable variation in PM severity ranged from 8.05 on Ottowa 770B to 97.02% on Cortland. Total soluble proteins, total phenols, antioxidant enzymes (peroxidase and polyphenoloxidase), ascorbic acid, tocopherol, and malondialdehyde (MDA), were determined in uninfected leaves of the tested cultivars. Pearson's correlation coefficient was calculated to measure the degree of association between PM severity and each component. All components showed significant (P < 0.05) or highly significant (P < 0.01) negative correlation with PM severity except MDA, which showed positive correlation (P < 0.01). Linear regression analysis was used to evaluate the causal relationship between the biochemical components (independent variables) and PM severity (dependent variable). Coefficient of determination ($R^2$) values of the generated models ranged from 48.76 to 77.15%. Tocopherol, MDA, and proteins were the most important contributors to the total variation in PM severity as the $R^2$ values of their models were 71.78, 75.28, and 77.15%, respectively. The results of the present study suggest that tocopherol, MDA, and proteins in uninfected leaves can be used as biochemical markers to predict PM resistance in flax.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.4
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• pp.261-269
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• 2021

Because sows are industrially vital for swine production, monitoring for their health or disorder status is important to ensure high reproductive performance. Especially, ambient temperature changes in different season, especially during summer, are directly influenced to the reproductive performance of sows. Although the serum biochemical parameters are widely applied in the veterinary medicine with wide ranges for the physiological process, the values are also influenced by several factors such as age, breed, gender, and stress. In addition, domestic sows in Korea-specific reference interval (RI) for serum biochemistry has not been established yet. Therefore, the present study was aimed to evaluate seasonal variation of RIs in the serum biochemistry in domestic sows in Korea at different seasons and to establish normal RIs using a RI finding program (Reference Value Advisor). Significant difference (p < 0.05) on the different seasons were identified in several serum biochemical parameters including BUN, CRE, GGT, GLU, ALB, TP, LDH and Na in sows. Therefore, we further established RIs, specific in domestic sows in Korea regardless of season. The established RIs based on the serum biochemical values provide a baseline for interpreting biochemical results in the domestic sows in Korea, regardless of seasonal effect. It may contribute to develop a strategy for better reproductive performance by improving breeding management practice and evaluating health of pig herds, which facilitate to avert the economic loss in summer infertility in sows.

• Journal of the Korean Home Economics Association
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• v.50 no.2
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• pp.39-51
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• 2012

The purpose of this study was to elucidate the general characteristics, antheropometric values and health-related characteristics of the rural elderly population according to their Sasang constitutions. The participants in this study were 31 in Taeeumin group, 29 in Soyangin group, and 15 in Soeumin group. The present study was performed using self-administration questionnaires, antheropometric and biochemical analysis. There were no significant differences in height, weight, BMI, diastolic blood pressure and alcohol habits between the Sasang constitutions. In the analysis of physical activity, Soyangin had a higher value than the other groups. Compared with the other groups, Soeumin also had considerable resistance to stress($101.1{\pm}26.2$ point). Furthermore, in the analysis of fatigability caused by stress, Soeumin was mostly shown to feel less fatigue than the other groups. On the other hand, Taeeumin appeared to feel more fatigue. In the biochemical analysis of blood, there was no significant difference between Sasang constitutions.

• YAKHAK HOEJI
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• v.38 no.6
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• pp.756-762
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• 1994

To investigate the acute toxicity of Elfvingia applanata$(P_{ers})K_{ARST}$, it was extracted with hot water. EA, the aqueous extract from the carpophores of E. applanata, was lyophilized and a dark brownish powder was obtained. The acute toxicity of EA was investigated in ICR mice of both sexes. Five groups of mice were administered orally and intraperitoneally with 375, 750, 1,500, 3,000, 6,000 mg/kg(p.o.) and 312.5, 625, 1,250, 2,500, 5,000 mg/kg (i.p.) of EA Abnormal clinical signs related to EA were not observed and no deaths occurred. $LD_{50}$ values administered orally and intraperitoneally were evaluated to be over 6,000 mg/kg and over 5,000 mg/kg, respectively. Gross finding of necropsy revealed no evidence of specific toxicity related to EA. There were no significant changes in body weights, serum biochemical values and histopathological changes compared with control group.

• Membrane and Water Treatment
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• v.9 no.6
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• pp.455-462
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• 2018

The measurement and monitoring of the biochemical oxygen demand (BOD) play an important role in the planning and operation of wastewater treatment plants. The most basic method for determining biochemical oxygen demand is direct measurement. However, this method is both expensive and takes a long time. A five-day period is required to determine the biochemical oxygen demand. This study has been carried out in a wastewater treatment plant in Turkey (Hurma WWTP) in order to estimate the biochemical oxygen demand a shorter time and with a lower cost. Estimation was performed using artificial neural network (ANN) method. There are three different methods in the training of artificial neural networks, respectively, multi-layered (ML-ANN), teaching learning based algorithm (TLBO-ANN) and artificial bee colony algorithm (ABC-ANN). The input flow (Q), wastewater temperature (t), pH, chemical oxygen demand (COD), suspended sediment (SS), total phosphorus (tP), total nitrogen (tN), and electrical conductivity of wastewater (EC) are used as the input parameters to estimate the BOD. The root mean squared error (RMSE) and the mean absolute error (MAE) values were used in evaluating performance criteria for each model. As a result of the general evaluation, the ML-ANN method provided the best estimation results both training and test series with 0.8924 and 0.8442 determination coefficient, respectively.

• Biomedical Science Letters
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• v.16 no.4
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• pp.317-322
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• 2010

This study has been done with 1,431 subjects who visited Health Promotion Centers of the hospitals in Gumi for National Health Screening Program for People at Transitional Ages from April to December 2007. Serum biochemical tests related with metabolic syndrome were performed. Among biochemical factors related with metabolic syndrome, the mean values of serum glucose, AST, ALT, triglyceride and HDL cholesterol except LDL cholesterol were significantly higher in males than in females, so a significant difference by sex was observed (P<0.001). AST, ALT, triglyceride and HDL were thought to be significantly affecting serum GGT for males. In contrast, ALT and HDL cholesterol were important factors for females (P<0.001). For both sexes, serum glucose and LDL cholesterol did not produce any meaningful effect on serum GGT. In males AST, ALT and HDL cholesterol were associated with high risk of abnormality of serum GGT and in females AST, ALT and LDL cholesterol were related with high risk of abnormality of serum GGT. Therefore, AST and ALT showed a significant effect on abnormality of serum GGT in both males and females. It was observed that males exhibited significantly high correlation between metabolic syndrome related biochemical factors and serum GGT than females, and their influence on abnormality of serum GGT was also higher in males than in females. Therefore, serum GGT tests performed for health screening are considered to be useful for managements of cardiovascular diseases and metabolic syndrome as well as liver function test.

• Journal of Veterinary Clinics
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• v.14 no.1
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• pp.131-135
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• 1997

Nutritional secondary hyperparathyroidism in a seven-month old male kitten showing signs of paraparesis (ataxia, dragging the pelvic limbs), abdominal distention, aconuresis was diagnosed with clinical signs, radiographs and serum biochemical tests. In radiographs, bones were abnormally radiolucent and cortices were thin. Serum biochemical tests were performed, but had normal values. The treatment was directed at the suspected dietary calcium and phosphorus imbalance. Oral calcium supplement and a commercial cat food were introduced. On clinical evaluation 3 months later, this case showed no clinical signs.

• Proceedings of KOSOMES biannual meeting
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• 2007.11a
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• pp.187-188
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• 2007

The fluctuations of biochemical and molecular activities III the harmful dinoflagellate, Cochlodinium polykrikoides, depending on water temperatures, were studied. In genomic DNA concentration, a similar value of 0.6 was shown at $12^{\circ}C$ and $15^{\circ}C$, but significantly increasing DNA from $18^{\circ}C$ (p<0.05), with a maximum of 1.8 at $24^{\circ}C$. After$24^{\circ}C$, the DNA significantly decreased to 0.6. Likely, the concentrations of RNA and total protein were at their highest values of 1.7 and 0.07 g $mL^1$ at $24^{\circ}C$, respectively. In contrast to ONA, RNA and total protein began to increase at $15^{\circ}C$. Oxygen availability between lower and higher temperatures was significantly different and increased from $18^{\circ}C$ according to light intensity, regardless of wavelengths (p<0.05). At $24^{\circ}C$, the highest value of the maximum electron transport rate (ETRmax), ranging from 537.9 (Ch 1) to 602.5 mol electrons $g^{-1}$ Ch1 a $s^{-1}$ (Ch 4), was also shown. Nitrate reductase (NR) and ATPase activities were at their highest values of 0.11 mol $NO_2^-g^{-1}$ Ch1 a $h^{-1}$ and 0.78 pmol 100 $mg^{-1}$ $at^2$ $4^{\circ}C$, respectively. When the cells cultured at $15^{\circ}C$, NR and ATPase activities significantly increased compared to $12^{\circ}C$ (p<0.05). In an analysis of CHN, the concentration of C and N also significantly increased (p<0.05). However, at $27^{\circ}C$, most of the molecular and biochemical movements were much lower, compared to $24^{\circ}C$. These results suggest that C. polykrikoides is very sensitive biochemical and molecular activities depending on water temperatures. Possibly, it is desirable to estimate at $18^{\circ}C$ the initiation of the massive blooming development of C. polykrikoides. In nature, it will be very difficult to maintain the massive blooms after $24^{\circ}C$ because of the possibility of significantly decreasing the molecular movement and activity of C. polykrikoides.

• Korean Journal of Veterinary Research
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• v.35 no.3
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• pp.437-445
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• 1995

This experiment was carried out to study the preventive effect of cysteine on the toxicities of captafol to the hematological and serum biochemical values. A single dose of captafol(5mg/kg BW, ip) was given to male Sprague-Dawley rats and its toxicities were evalutated by body weitht changes, autopsy findings, absolute organ weight, and hematological and serum biochemical parameters. The single dose of captafol caused significant decreases in body weitht, and absolute liver weight, as-cites, fibrin clot in abdominal cavity, adhesion of liver lobes significant elevation of number of RBC, hemoglobin concentration and serum AST activity, and decreased of serum phospholipid level. Where as cysteine(over 58mg/kg BW) given immediately after captafol appeared to prevent the ascites, fibrin clot in abdominal cavity and liver lobe adhesion. It also prevented the liver and blood, especially RBC toxicites. The results suggest that cysteine and other compounds containing sulfhydryl groups may protect the subjects from captafol-induced toxicity.