• Title/Summary/Keyword: biochemical tests

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Biochemical properties and cultural characteristics of Escherichia coli isolated from chickens (닭에서 분리한 Escherichia coli의 생물화학적 및 배양 특성)

  • Woo, Yong-ku;Kim, Ki-seuk;Kim, Bong-hwan
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.421-425
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    • 1990
  • The present study was conducted to investigate the biochemical and cultural characteristics of Escherichia coli isolates from clinically affected chickens during the period from May 1988 to June 1989. A total of 82 E coli cultures were isolated from lesions of 75 chickens with colisepticemia. Biochemical properties of E coli isolates tested were in accordance with the general classification standard; all the isolates showed positive reaction in Catalase, Indol, and Methyl-Red tests, but negative reaction in Oxidase, Urease, $V{\ddot{o}ges$-Proskauer, Citrate utility, $H_2S$, Phenylalanine diaminase, and malonate tests. And the carbohydrate fermentation rates of them were shown to be variable. of the 82 isolates, 48(58.5%) cultures produced colicin to inhibit the indicator strain of E coli.

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A Case of a Kitten with Nutritional Secondary Hyperparathyroidism (새끼 고양이에서의 영양학적 원인에 의한 부갑상선 기능항진증 증례)

  • 박우대;김휘율;배춘식;김희정;윤화중;장경진
    • Journal of Veterinary Clinics
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    • v.14 no.1
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    • pp.131-135
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    • 1997
  • Nutritional secondary hyperparathyroidism in a seven-month old male kitten showing signs of paraparesis (ataxia, dragging the pelvic limbs), abdominal distention, aconuresis was diagnosed with clinical signs, radiographs and serum biochemical tests. In radiographs, bones were abnormally radiolucent and cortices were thin. Serum biochemical tests were performed, but had normal values. The treatment was directed at the suspected dietary calcium and phosphorus imbalance. Oral calcium supplement and a commercial cat food were introduced. On clinical evaluation 3 months later, this case showed no clinical signs.

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Metabolic Syndrome Related with Serum Biochemical Factors Affecting Serum GGT at Transitional Ages in Gumi

  • Seok, Seong-Ja
    • Biomedical Science Letters
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    • v.16 no.4
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    • pp.317-322
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    • 2010
  • This study has been done with 1,431 subjects who visited Health Promotion Centers of the hospitals in Gumi for National Health Screening Program for People at Transitional Ages from April to December 2007. Serum biochemical tests related with metabolic syndrome were performed. Among biochemical factors related with metabolic syndrome, the mean values of serum glucose, AST, ALT, triglyceride and HDL cholesterol except LDL cholesterol were significantly higher in males than in females, so a significant difference by sex was observed (P<0.001). AST, ALT, triglyceride and HDL were thought to be significantly affecting serum GGT for males. In contrast, ALT and HDL cholesterol were important factors for females (P<0.001). For both sexes, serum glucose and LDL cholesterol did not produce any meaningful effect on serum GGT. In males AST, ALT and HDL cholesterol were associated with high risk of abnormality of serum GGT and in females AST, ALT and LDL cholesterol were related with high risk of abnormality of serum GGT. Therefore, AST and ALT showed a significant effect on abnormality of serum GGT in both males and females. It was observed that males exhibited significantly high correlation between metabolic syndrome related biochemical factors and serum GGT than females, and their influence on abnormality of serum GGT was also higher in males than in females. Therefore, serum GGT tests performed for health screening are considered to be useful for managements of cardiovascular diseases and metabolic syndrome as well as liver function test.

An investigation of lead absorption in an electric accumulator factory with the use of personal samplers

  • Williams M.K.;King E.;Walford Joan
    • 대한예방의학회:학술대회논문집
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    • 1994.02a
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    • pp.561-575
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    • 1994
  • Williams, M K., King, E., and Walford, Joan (1969). Brit. J. industr. Med., 26, 202-216. An investigation of lead absorption in an electric accumulator factory with the use of personal samplers. Thirty-nine lead workers and controls, in stable conditions of exposure, each wore personal lead-in-air samplers daily for two weeks. During the second week samples for blood lead, urinary lead, urinary coproporphyrin, urinary $\gamma$-aminolevulinic acid (ALA), the punctate basophil count, and haemoglobin were taken daily. Duplicate estimations were made on one day. The lead exposures of men doing almost identical jobs differed by ratios of up to four to one. This could be attributed on y to personal differences in working habits. The correlation coefficients and regression equations of the biochemical tests with lead-in-air and with each other were determined. The mean values and $95\%$ confidence limits of single determinations of some of the biochemical tests corresponding to the two commonly accepted TLVs of lead-in-air (0-20 and 0-15 mg./$m^{3}$) were calculated from the regression equations. For each biochemical test the variation due to analytical error, the variation from day to day within subjects and the residual variation about the regression on lead-in-air were calculated. Previous estimates of the latter are not known. Excessive confidence may be placed in an index of exposure due to its low coefficient of variation within subjects unless the coefficient of variation between subjects about regression is taken into account. The correction for specific gravity of estimations of lead and ALA in spot samples of urine was found to reduce slightly the residual variation between subjects about the regression on lead-in-air and to increase the correlations with lead-in-air and with the other biochemical tests, but these changes were not statistically significant. The modified method used for estimating blood lead and urinary lead is described and validated.

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Analysis on the Findings of Serum Biochemical Test in Inhabitants Infected with Clonorchis sinensis

  • Sohn, Woon-Mok;Cho, Young-Pyo;Kim, Kee-Jin;Kim, Mi-Young;Lee, Chong-Wook;Park, Do-Hum
    • Biomedical Science Letters
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    • v.7 no.1
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    • pp.39-45
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    • 2001
  • The findings of serum biochemical tests in the inhabitants residing in some riverside areas of kyongsangnam-do were analized to know the correlation with the Clonorchis sinensis infection. A total of 2,722 fecal samples of inhabitants was examined by Kato's cellophane thick smear and formalin-ether sedimentation methods, and their serums were analized biochemically. Three hundred and thirty-three inhabitants (12.2%) were positive with C. sinensis eggs (egg-positive group), and remaining 2,389 were negative (egg-negative group). In the egg-positive group, the positive .ate of aspartate aminotransferase (AST) was 6.9% (male: 10.3%; female: 3.2%), that of alanine aminotransferase (ALT) was 6.3% (male: 9.1%; female: 3.2%), of $\gamma$-glutamy1 transpeptidase ($\gamma$-GIP) was 9.0% (male: 13.1%; female: 4.4%), and that of $\alpha$-fetoprotein (AEP) was 3.0% (male: 2.9%; female: 3.2%). In the egg-negative group, the positive rate of AST was 5.2% (male: 8.9%; female: 3.2%), that of ALT was 5.1% (male: 8.6%; female: 3.2%), of $\gamma$-GTP was 7.5% (male: 13.0%; female: 4.5%), and that of AFP was 0.3% (male: 0.5%; female: 0.1%). In the egg-positive group, 127 cases (38.1%) were light infection, 108 (32.4%) were moderate infection,64 (19.2%) were heavy infection, and 34 (10.2%) were very heavy infection. The positive rates of all tests were high according to the intensity of infection. From the above results, It was confirmed that prevalence of clonorchiasis is still high in some riverside areas of Kyongsangnam-do, and findings of serum biochemical tests are not different between the egg-positive group and egg-negative group. However, there were some difference in the positive rates of serum AST, ALT, $\gamma$-GTP and AEP by the age, sex and the intensity of infection.

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Strain Improvement of Candida tropicalis for the Production of Xylitol: Biochemical and Physiological Characterization of Wild-type and Mutant Strain CT-OMV5

  • Rao Ravella Sreenivas;Jyothi Cherukuri Pavanna;Prakasham Reddy Shetty;Rao Chaganti Subba;Sarma Ponnupalli Nageshwara;Rao Linga Venkateswar
    • Journal of Microbiology
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    • v.44 no.1
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    • pp.113-120
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    • 2006
  • Candida tropicalis was treated with ultraviolet (UV) rays, and the mutants obtained were screened for xylitol production. One of the mutants, the UV1 produced 0.81g of xylitol per gram of xylose. This was further mutated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and the mutants obtained were screened for xylitol production. One of the mutants (CT-OMV5) produced 0.85g/g of xylitol from xylose. Xylitol production improved to 0.87 g/g of xylose with this strain when the production medium was supplemented with urea. The CT-OMV5 mutant strain differs by 12 tests when compared to the wild-type Candida tropicalis strain. The XR activity was higher in mutant CT-OMV5. The distinct difference between the mutant and wild-type strain is the presence of numerous chlamvdospores in the mutant. In this investigation, we have demonstrated that mutagenesis was successful in generating a superior xylitol-producing strain, CT-OMV5, and uncovered distinctive biochemical and physiological characteristics of the wild-type and mutant strain, CT-OMV5.

Acute and Subacute Toxicity Study of Artemisia asistica Nakai Aqua-acupuncture Solution in Mice (애엽(艾葉) 약침액(藥鍼液)의 급성(急性)·아급성(亞急性) 독성(毒性)에 관한 연구(硏究))

  • Youn, Seong-Muk;Lim, Jong-Kook
    • Journal of Acupuncture Research
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    • v.17 no.1
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    • pp.143-151
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    • 2000
  • Acute and subacute toxicity of Artemisia asistica Nakai Aqua-acupuncture Solution (ANAS) were studied in ICR mice. In acute toxicity test, mice were injected intraperitoneally with single dose of $1{\times}$, $5{\times}$, $10{\times}$ ANAS, and toxicological responeses were observed for consecutive 14 days. Mortality, body weight changes, organ weight, and serum chemistry were performed. The mortality and body weight changes of mice treated with $1{\times}$ and $5{\times}$ ANAS were not affected during the experimental periods. With the $10{\times}$ ANAS treatment, there were dead animals and changes of body weight, organ weight and serum biochemical values were observed during the experimental period. In subacute toxicity test, mice were injected intraperitoneally with doses of $1{\times}$, $10{\times}$ ANAS for 14 days. No difference was found between control and $1{\times}$ ANAS treated group in mortality, changes of body weight and organ weight, and serum biochemical values. However, Dead animals, changes of body weight and organ weight, and increased serum biochemical values were observed with $10{\times}$ ANAS treated groups. These results suggest that $1{\times}$ ANAS causes no toxicity in acute and subacute toxicity tests. However $10{\times}$ ANAS causes toxicity in both tests.

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The Biochemical Characteristics of Acetobacter sp. Isolated from Persimmen Vinegar (감식초에서 분리한 Acetobacter sp.의 생화학적 특성)

  • 이상복;전승호
    • Journal of environmental and Sanitary engineering
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    • v.14 no.4
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    • pp.180-187
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    • 1999
  • The following is experimental result of Biochemical characteristics of Acetobacter sp. Acetobacter sp. were gram negative, short rod, non-spore-forming and motile. It reacted positively catalase, methyl red, oxidation fementation, voges-proskauer and nitrate reduction tests and hydrogen sulfide test negative and ONPG negative. Acetobacter sp. showed normal growth curve in Carr broth and there was no significant difference between isolates and type strains such as Acetobacter aceti(KCTC 1010), Acetobacter diazotrophicus(KCTC 2859). Acetobacter liquefaciens(KCTC 2804) and Acetobacter pasteurianus(KCTC 1008).

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Antigenicity Studies of the Aqueous Extract of Fresh Ginseng in Guinea Pigs

  • Lee, Jong-Wha;Rhee, Man-Hee;Park, Jong-Il;Han, Sang-Seop
    • Korean Journal of Pharmacognosy
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    • v.25 no.1
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    • pp.35-40
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    • 1994
  • Aqueous extreact of fresh ginseng (AFG) was examined for the antigenicity in Hartley guinea pigs in comparision with ovalbumin (OVA). When guinea pigs were sensitized with AFG emulsified with complete Freund's adjuvant (CFA), these animals showed negative reactions in active systemic anaphylaxis (ASA), active cutaneous anaphylaxis (ACA) and passive cutaneous anaphylaxis (PCA) tests and passive hemagglutination (PHA) reaction. On the contrary, when guinea pigs were sensitized with OVA emulsified with CFA as positive controls, these animals disclosed positive reactions in ASA, ACA and PCA tests and PHA reaction. From these results, AFG was considered not to possess antigenic properties in guinea pigs. In addition, the dose levels of AFG empolyed in the present experiment were confirmed not to suppress immune reactions.

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Biochemical properties and antimicrobial drug susceptibility of Erysipelothrix rhusiopathiae isolated from the cases of acute septicemic swine erysipelas (급성 폐혈증형 돼지단독예에서 분리한 Erysipelothrix rhusiopathiae의 생화학적 특성 및 약제감수성)

  • Baek, Young-sook;Cho, Gil-jae;Kim, Bong-hwan
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.413-420
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    • 1990
  • The present study was conducted to investigate biochemical properties and antimicrobial drug susceptibilities of 47 strains of E rhusiopathiae isolated from the cases of acute septicemic swine erysipelas in Youngnam and Kyunggi provinces during the period from June 1988 to December 1989. The isolants were identified as E rhusiopathiae on the basis of cellular and colonial morphology, and characteristic reactions in some biochemical tests. All the organisms produced hydrogen sulfide in triple sugar iron agar and showed the characteristic "pipe cleaner" type of growth in gelatin stab cultures. The majority of biochemical and cultural properties of E rhusiopathiae isolated from pigs affected with acute erysipelas were identical to those of the reference strains employed. All the isolates were highly susceptible to penicillin G, ampicillin, erythromycin (MIC:0.025~0.39IU or ${\mu}g/ml$), and moderately susceptible to oleandomycin, oxytetracycline, chloramphenicol(MIC:$0.78{\sim}25{\mu}g/ml$). Kanamycin and sulfadimethoxine showed no activity against the isolates(MIC:>$400{\mu}g/ml$). The MICs of dihydrostreptomycin presented two distribution peaks; of 47 strains, 5(10.6%) were resistant to dihydrostreptomycin (MIC:$400{\mu}g/ml$), while the majority of them were susceptible to the drug.

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