• KSBB Journal
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• 제23권4호
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• pp.311-316
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• 2008

본 연구는 제주도 근해에 자생하는 참가죽그물바탕말 (Dictyota coriacea)의 에탄올 추출물 및 순차적 용매분획물을 얻어 B16F10 mouse melanoma 세포및 RAW 264.7 세포에 처리하여 멜라닌 생합성 및 tyrosinas 억제활성, NO 억제활성을 통하여 유용자원으로서 미백 및 기능성 화장료로 산업적 활용 가능성이 있는지 알아보고자 연구를 시행하였다. 실험한 결과 B16F10 세포에서 참가죽그물바탕말 에탄올 추출물과 헥산 분획물, 에틸아세테이트 분획물이 tyrosinase와 멜라닌 합성을 농도 의존적으로 뛰어나게 억제하였으며, 단백질 및 mRNA 발현양이 tyrosinase와 TRP-1 유전자에서 농도 의존적으로 감소함을 보였다. 하지만 TRP-2 단백질과 mRNA 유전자의 발현을 확인한 결과, TRP-2 단백질과 mRNA 발현이 농도에 관계없이 일정하였다. 또한 LPS로 자극한 RAW 264.7 세포에서 참가죽그물바탕말의 에탄을 추출물과 헥산 분획물, 에틸아세테이트 분획물이 가장 높은 NO 생성 억제 활성을 보였다. 따라서 본 연구결과는 참가죽그물바탕말 추출물이 항염증 활성을 가지는 미백 관련 기능성 소재로서의 활용가치가 있음을 보여준다.

• 농촌계획
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• 제13권2호
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• pp.1-5
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• 2007

Shannon and Simpson indexes are used for species diversity analysis of ecosystem. In species diversity analysis of ecosystem, not only frequency of each species but also survey size have to be considered. In this study, total information composed with knowledge and ignorance was suggested as a species diversity analysis method for ecosystem survey. To apply developed method, flora in the Sangachun river valley was sampled with 19 sites and 198 species. In applying results, Shannon index shows more reasonable results than Simpson index by the variance of sample size but has difficulties of determining the relation of surveying species number and sample site number. Suggested total information can overcome this difficulty by the relation of knowledge and ignorance.

• 생약학회지
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• 제36권1호통권140호
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• pp.50-55
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• 2005

Reactive oxygen species (ROS) are continuously produced at a high rate as a by-product obtained in the aerobic metabolism. A major portion of living organisms has defense system as superoxide dismutase or catalase against damage produced by ROS. Several lines of evidence provided that ROS appears to cause to develop aging and various diseases. Spatholobus suberectus Dunn has been known as a Korean folk medicine for promoting blood circulation and relieving blood stasis as anemia. In this study, we have investigated the antioxidant activities of Spatholobus suberectus Dunn in order to find the antioxidant substances from natural products. For various antioxidant experiment, the major antioxidant component was isolated from EtOAc extract of Spatholobus suberectus Dunn. This compound was identified as (-)-epicatechin by chemical and physical analysis.

• 생약학회지
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• 제35권3호통권138호
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• pp.184-188
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• 2004

This study was carried out to evaluate glutathione S-transferase (GST) activity and hyaluronidase inhibitory effect of medicinal plants. The EtOH extracts of 20 species plants were tested. As the result, Acorus gramineus and Pueraria lobata exhibited GST activity. On the continuous experiment, the n-BuOH fraction of Acorus gramineus and the $H_2O$ fraction of Pueraria lobata showed the elevation of GST activity. On the experiment of hyaluronidase inhibitory effect, Acorus gramineus exhibited a potent inhibitory activity. These results suggest that the extract of Acorus gramineus can be applicable for the development of a new anti-inflammatory agent.

• Food Science and Biotechnology
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• 제17권6호
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• pp.1160-1164
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• 2008

An novel amylolytic yeast, Saccharomyces cerevisiae HA 27, isolated from nuruk, displayed resistance against high sugar (50% glucose) and alcohol (15%). Maximal production of amylolytic enzyme by S. cerevisiae HA 27 was achieved on 9 days of cultivation at the optimal temperature $20^{\circ}C$ and pH 6.0. The activity of amylolytic enzyme produced by S. cerevisiae HA 27 was stable, even at $70^{\circ}C$, and over a broad pH range (4.0-11.0). Also, the amylolytic enzyme of S. cerevisiae HA 27 showed optimal activity in pH 5.0 at $50^{\circ}C$. S. cerevisiae HA 27 exhibited 6.2%(v/v) alcohol fermentation ability using starch as a carbon source.

• 한국자기공명학회논문지
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• 제13권2호
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• pp.96-107
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• 2009

Lactophoricin (LPcin-I) is a cationic amphipathic peptide with 23-mer peptide, and corresponds to the carboxy terminal 113-135 region of Component-3 of proteose-peptone. LPcin-I is a good candidate as a peptide antibiotic, because it has an antibacterial activity, but no hemolytic activity. On the other hand, its shorter analog (LPcin-II), which corresponds to the 119-135 region of PP3, has no antibacterial activity. In order to understand the structure-activity relationship under the membrane environments, we succeed to produce large amounts of LPcin-I and LPcin-II peptides. Peptides were over expressed in the form of fusion protein in Escherichia coli, and purified with several chromatography techniques. In this paper, we introduce the optimizing processes of purification and NMR measurement.

• Journal of Applied Biological Chemistry
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• 제62권2호
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• pp.149-155
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• 2019

Elicitor treatment was performed to increase the anti-inflammatory activity of useful biological sources. The result showed that elicitor-treated Saururus chinensis leaf extracts positively affected nitric oxide (NO) production, and the expression of inducible NO synthase and cyclooxygenase-2 compared to extracts not exposed to elicitor treatment. This finding identified elicitor treatment as a suitable strategy for increasing the biological activity of S. chinensis. Therefore, elicitor-treated S. chinensis is useful both as health functional and medicinal materials.

• 대한화학회지
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• 제65권3호
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• pp.234-239
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• 2021

• 한국동물위생학회지
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• 제43권2호
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• pp.107-112
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• 2020

Foot-and-mouth disease (FMD) and avian influenza (AI) are highly pathogenic viral disease which affects the livestock industry worldwide. Outbreak of these viruses causes great impact in the livestock industry; thus, disease infected animals were immediately disposed. Burial is the commonly used disposal method for deceased animals. However, there is potential for secondary environmental contamination, as well as the risk that infectious agents persisting in the environment due to the limited environmental controls in livestock burial sites during the decomposition of the carcasses. Therefore, this study aimed to investigate the detection of FMD and AI viruses from animal carcass disposal sites using real-time reverse transcription PCR. Soil samples of more than three years post-burial from livestock carcass disposal sites were collected and processed RNA isolation using a commercial extraction kit. The isolated RNA of the samples was used for the detection of FMDV and AIV using qRT-PCR. Based on the qPCR assay result, no viral particle was detected in the soil samples collected from the animal disposal sites. This indicates that 3 years of burial and their carcass disposal method is efficient for the control or at least reduction of spread infections in the surrounding environment.

• 융합정보논문지
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• 제11권1호
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• pp.175-182
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• 2021

본 연구의 목적은 디지털바이오헬스케어산업의 연관산업에 대한 고용유발효과를 시기별로 비교 분석하여 효과적인 산업정책을 수립하기 위한 것이다. 연구를 위한 기본데이터는 한국은행이 실측했던 3개년의 산업연관표를 활용하였다. 연구 방법은 첫째, 산업분류 기준이 다른 시기별 통계자료들을 비교분석하기 위해 7대 산업으로 재작성하였다. 둘째, 한국은행의 산업연관분석 방법론으로 비교분석하였다. 셋째, 격차 조정을 위해 가중치를 반영하여 디지털바이오헬스케어산업의 고용, 생산, 투자 부문에서 유발 효과들을 비교분석하였다. 분석 결과는 첫째, 고용 부문에서는 전체 산업의 평균보다 높은 효과가 있고, 둘째, 생산 부문에서는 낮았고, 셋째, 투자 부문에서는 서비스 분야의 투자가 필요하였다. 분석에서 도출된 결론은, 고용 부문에서 직접투자 및 지속적인 투자가 필요하고, 전문 인력의 육성이 시급하며, 생산 부문에서는 직접투자와 장기투자가 효과적인 것으로 나타났다.