• 한국동물위생학회지
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• 제29권1호
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• pp.27-36
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• 2006

This studies were carried out to investigate the rearing managements of pig farms and survey on pneumonia of the slaughtered pigs from 5 selected herds located in Jangsu, Jeonbuk. Isolated aerobic microorganisms from pneumonic lung were examined antibiotic susceptibility and tested serological antibody titers of the herd base. Prevalence rate of pneumonia were examined according to rearing and health management conditions of pig farms. Prevalence rate of pneumonia were detected in 78.8% and enzotic pneumonia. In 47.7%, pleuropneurnonia in 31.1%. In serological antibody titers showed the positive reaction with 54.4% in Actinobacillus pleuropneumonia serotype 5, 44.8% in Pasteurella multocida, 36.8% in A pleuropneurnonia serotype 2, 13.6% in Mycoplasmal pneumonia. Isolated aerobic microorganisms were examined antibiotic susceptibility and showed the high activity in gentamicin (58.3%), enrofloxacin (53.3%), norfloxacin (51.6%), cephalothin (41.7%) and low activity in amoxycillin (98.3%), oxytetracycline (98.3%), penicillin G (90.0 %), tetracycline (88.4%), ampicillin (88.3%). Farm managements were deficient effect of humidity in swine house but ammonia gas all appeared the 10 ppm that were recommendation density, below.

• IMMUNE NETWORK
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• 제22권1호
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• pp.4.1-4.22
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• 2022

In the era of immunotherapeutic control of cancers, many advances in biotechnology, especially in Ab engineering, have provided multiple new candidates as therapeutic immuno-oncology modalities. Bispecific Abs (BsAbs) that recognize 2 different antigens in one molecule are promising drug candidates and have inspired an upsurge in research in both academia and the pharmaceutical industry. Among several BsAbs, T cell engaging BsAb (TCEB), a new class of therapeutic agents designed to simultaneously bind to T cells and tumor cells via tumor cell specific antigens in immunotherapy, is the most promising BsAb. Herein, we are providing an overview of the current status of the development of TCEBs. The diverse formats and characteristics of TCEBs, in addition to the functional mechanisms of BsAbs are discussed. Several aspects of a new TCEB-Blinatumomab-are reviewed, including the current clinical data, challenges of patient treatment, drawbacks regarding toxicities, and resistance of TCEB therapy. Development of the next generation of TCEBs is also discussed in addition to the comparison of TCEB with current chimeric antigen receptor-T therapy.

• 한국가금학회지
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• 제43권3호
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• pp.143-148
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• 2016

본 시험은 오리 사료 내 난황항체제제 첨가 시 생산성, 혈액 특성, 장내 미생물 및 면역글로불린에 미치는 영향을 조사하기 위하여 수행하였다. 1일령 오리(Cherry valley) 총 300수를 공시하여 5처리 3반복, 반복당 20수씩 공시하여 6주간 시험을 실시하였다. 처리구는 항생제를 첨가하지 않은 처리구(NC, negative control), 항생제 처리구(PC, positive control), 난황항체제제 0.1% 첨가구(T1), 난황항체제제 0.5% 첨가구(T2) 그리고 난황항체제제 1.0% 첨가구(T3)로 나누어 실험을 실시하였다. 종료 시 체중은 난황항체제제 첨가구와 항생제첨가구가무항생제처리구보다 유의적으로 높았고(P<0.05), 증체량 역시 난황항체제제 첨가구와 항생제 첨가구가 무항생제 처리구에 비해 높게 나타났다(P<0.05). 하지만 전 기간에 대한 사료 섭취량과 사료 요구율은 처리구간에 통계적인 유의차가 나타나지 않았다. 6주간 사양 T실험 후 혈액 생화학 및 혈구 분석 결과, 난황항체제제 0.1, 1.0% 첨가구에서는 total cholesterol 함량이 무항생제 처리구에 비해 다소 감소하는 경향을 보였으나, 통계적인 유의차는 없었다. 또한 AST와 ALT 함량 역시 처리구 간에 유의적인 차이를 보이지 않았다. 오리의 맹장 내 유산균 수는 난황항체제제 0.1와 0.5% 급여 시 다른 처리구에 비해 가장 높았으며(P<0.05), 대장균 수는 난황항체제제 수준이 증가함에 따라 감소하는 경향을 보였으나, 통계적 유의차는 없었다. 또한 혈청 내 면역글로불린 함량에도 처리구간에 유의적인 차이는 나타나지 않았다. 결과적으로 오리 사료 내 난황항체첨가 시 오리의 생산성을 개선시키고, 장내 미생물 균총에 효과적인 것으로 나타났으나, 아직까지 국내에서는 난황항체제제 첨가에 따른 사양 시험과 관련된 연구가 부족하다는 점을 감안할 때 향후 이에 대한 많은 연구가 필요할 것으로 사료된다.

• 한국미생물·생명공학회지
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• 제17권5호
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• pp.489-493
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• 1989

쥐 하이브리도마 Alps 25-3를 이용하여 무혈청 배지 KM3의 각 성분이 세포증식과 항체생성에 미치는 영향을 조사하여 무혈청 배지를 최소화한 KMS를 결정하였다. Alps 25-3에 영향을 미치는 것으로 조사된 transferrin, ethanolamine 그리고 BSA만을 혼합하여 결정한 KMS를 다른 쥐 하이브리도마인 A4W, KW 그리고 HCGK에 적용시켰다. KM5를 KM3와 비교하였을 때 세포증식과 항체생산이 거의 동일한 양상을 보였다. 그러나 혈청배지와 비교하여 보면 최고 세포농도는 50-90%, 최종 단일클론항체의 농도는 85-100%의 수준을 보였다. 본 연구의 결과로 쥐 하이브리도마 세포를 무혈청 배지에서 배양시킬 때 필수적으로 첨가기어야 하는 성분들이 조사되었으므로 대량배양에 응용하거나 대사를 연구하는데 유용하게 이용되어지리라 본다.

• 한국어병학회지
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• 제7권2호
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• pp.119-126
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• 1994

넙치의 면역반응에 수온이 미치는 영향을 조사하기 위하여 넙치에 포르말린 사균 Edwardsiella tarda 로 주사면역시킨 후 수온변화에 따른 체액성면역반응을 관찰하였다. 저수온 (12, $15^{\circ}C$) 에서 항체는 면역 2~3 주 후에 나타났으며 최대 항체가는 각각 16 과 32 이었다. 그러나 고수온(20, $23^{\circ}C$) 에서는 면역 1 주후에 나타났으며 최대 항체가는 약 2,048 로 높았다. 그리고 한번 형성된 항체는 수온의 변화에 민감하게 반응하였고 자연조건에서도 유사한 경향을 보였으며 면역지속기간은 19 개월 이상으로 나타났다.

• 한국어병학회지
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• 제27권2호
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• pp.99-105
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• 2014

Biosensors consist of biochemical recognition agents like antibodies immobilized on the surfaces of transducers that change the recognition into a measurable electronic signal. Here we report a piezoelectric immunosensor made to detect Vibrio vulnificus. A 9MHz AT-cut piezoelectric wafer attached with two gold electrodes of 5mm diameter was used as the transducer of the QCM biosensor with a reproducibility of ${\pm}0.1Hz$ in frequency response. We have tried different approaches to immobilize antibody on the sensor chip. Concerning the orientation of antibody for the best antigen binding capacity, the antibody was immobilized by specific binding to protein G or by cross-linking through hydrazine. In addition, protein G was cross-linked on glutaraldehyde activated immine layer (PEI) or EDC/NHS activated sulfide monolayer (MPA). PEI was found to be more effective to immobilize protein G following glutaraldehyde activation than MPA. However, hydrazine chip showed a better capability to immobilize more IgG than protein G chip and a higher sensitivity. The sensor system was able to detect V. vulnificus in dose dependent manner and was able to detect bacterial cells within 5 minutes by monitoring frequency shifts in real time. The detection limit can be improved by preincubation to enrich the bacterial cell number.

• 대한수의학회지
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• 제45권2호
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• pp.191-197
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• 2005

An immunochromatographic (IC) strip for the rapid detection of Salmonella spp. in the enriched sample was developed. Affinity purified Salmonella polyclonal antibody was conjugated with 40 nm colloidal gold particles which were prepared by citrate method in our laboratory. The antigen-antibody-gold complex was captured by Salmonella antibody attached to test line of nitrocellulose membrane during the capillary migration of sample. Specificity of the IC strip was calculated to be 100% (12/12) and sensitivity was 97.6% (41/42) in the test with pure cultured bacteria. Salmonella was artificially inoculated into raw pork macerated with enrichment broth. And then it was 10-fold diluted from $5.2{\times}10^{8}CFU/ml$ to 5.2 CFU/ml. The IC strip could detect $5.2{\times}10^{6}CFU/ml$ before enrichment. However, the lowest limit of detection was 5.2 CFU/ml after overnight incubation. The results indicated that the IC assay was a rapid, economical and simple method with high specificity and sensitivity for the detection of Salmonella spp. without using any equipment.

• 한국동물위생학회지
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• 제23권3호
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• pp.271-279
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• 2000

This study was carried out to provide the fundamental information for development of proper vaccination program against infectious bursal disease(IBD) to the local chicken farms. The antigen detection was peformed from 8 samples of bursa of Fabricius with agar gel precipitation(AGP) and indirect immunofluorescent assay(IFA), And also, the antibodies in serum samples were detected by the various serological methods such as commercial ELISA assay, AGP and virus neutralization(VN) test. 1. The antigen detection rates were 25% for AGP which is 2 out of 8 farms and 10 out of 40 bursas, and 25% which Is 2 out of 8 farms and 20% 8 out of 40 bursas for IFA, respectively. 2. The mean titer of maternal antibody (>3,000) existed until 10 days of the age with ELISA-GMT. 3. The antibody positive rates which are over 80% showed until 5 days of the age with ELISA and at 10 days of the age with AGP except one, but none of them showed from 1 day of the age. This report came to conclusions that both the protective maternal antibody titers and the antigen positive rates were significant until at the 10 days of the age.

• Molecules and Cells
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• 제27권2호
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• pp.225-235
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• 2009

Antibody phage display provides a powerful and efficient tool for the discovery and development of monoclonal antibodies for therapeutic and other applications. Antibody clones from synthetic libraries with optimized design features have several distinct advantages that include high stability, high levels of expression, and ease of downstream optimization and engineering. In this study, a fully synthetic human scFv library with six diversified CDRs was constructed by polymerase chain reaction assembly of overlapping oligonucleotides. In order to maximize the functional diversity of the library, a ${\beta}$-lactamase selection strategy was employed in which the assembled scFv gene repertoire was fused to the 5'-end of the ${\beta}$-lactamase gene, and in-frame scFv clones were enriched by carbenicillin selection. A final library with an estimated total diversity of $7.6{\times}10^9$, greater than 70% functional diversity, and diversification of all six CDRs was obtained after insertion of fully randomized CDR-H3 sequences into this proofread repertoire. The performance of the library was validated using a number of target antigens, against which multiple unique scFv sequences with dissociation constants in the nanomolar range were isolated.

• 방사선산업학회지
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• 제17권4호
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• pp.327-332
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• 2023

This study identified effects of Isoegomaketone for applicable patch on the arthritis in mice. Isoegomaketone (IK) was isolated from Perilla frutescens, which is annual herbal traditional medicinal, aromatic, functional food. IK has various physiological effects such as anti-inflammation, anti-oxidant, and anti-cancer. In the previous study, oral administration of IK to a mouse model of collagen antibody-induced arthritis(CAIA), which is similar to human rheumatoid arthritis(RA), alleviated symptoms. In this study, we attached a patch containing IK to mouse skin to demonstrate whether it had the same efficacy as oral administration in CAIA mouse. As a result of measuring the arthritis score, paw volume, and paw thickness, it was confirmed that arthritis symptoms were alleviated in the group to which the patch containing IK was attached. These results show that IK is effective in alleviating arthritis not only through oral administration but also through patches applied to skin, and that it has potential as a material for future patch development.