The serological studies on infectious bursal disease

전염성 F낭병에 대한 혈청학적 연구

  • 정영미 (전라북도축산진흥연구소) ;
  • 서석열 (전라북도축산진흥연구소) ;
  • 도홍기 (전라북도축산진흥연구소) ;
  • 조정곤 (전북대학교 생체안전성연구소) ;
  • 노수일 (전라북도축산진흥연구소)
  • Published : 2000.09.01

Abstract

This study was carried out to provide the fundamental information for development of proper vaccination program against infectious bursal disease(IBD) to the local chicken farms. The antigen detection was peformed from 8 samples of bursa of Fabricius with agar gel precipitation(AGP) and indirect immunofluorescent assay(IFA), And also, the antibodies in serum samples were detected by the various serological methods such as commercial ELISA assay, AGP and virus neutralization(VN) test. 1. The antigen detection rates were 25% for AGP which is 2 out of 8 farms and 10 out of 40 bursas, and 25% which Is 2 out of 8 farms and 20% 8 out of 40 bursas for IFA, respectively. 2. The mean titer of maternal antibody (>3,000) existed until 10 days of the age with ELISA-GMT. 3. The antibody positive rates which are over 80% showed until 5 days of the age with ELISA and at 10 days of the age with AGP except one, but none of them showed from 1 day of the age. This report came to conclusions that both the protective maternal antibody titers and the antigen positive rates were significant until at the 10 days of the age.

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