Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Egg Yolk Antibody Powder (IgY) Supplementation on Growth Performance, Blood Component Profile, Intestinal Microflora, and Immunoglobulin G in Meat Ducks

난황항체제제(IgY) 첨가가 육용오리의 생산성, 혈액성상, 장내 미생물 및 면역글로불린 함량에 미치는 영향

  • Jeon, Ik Soo (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Kang, Hwan Ku (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Kim, Chan Ho (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Hwangbo, Jong (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science) ;
  • Park, Seong Bok (Poultry Science Division, Livestock Resource Development, National Institute of Animal Science)
  • 전익수 (농촌진흥청 국립축산과학원 가금과) ;
  • 강환구 (농촌진흥청 국립축산과학원 가금과) ;
  • 김찬호 (농촌진흥청 국립축산과학원 가금과) ;
  • 황보종 (농촌진흥청 국립축산과학원 가금과) ;
  • 박성복 (농촌진흥청 국립축산과학원 가금과)
  • Received : 2016.05.31
  • Accepted : 2016.07.19
  • Published : 2016.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the effect of dietary egg yolk antibody (IgY) powder supplementation on the growth performance, blood component profile, intestinal microflora, and immunoglobulin G in ducks. A total of 300 1-day-old ducks (Cherry Valley) were randomly divided into 5 groups, with 3 replicates of 20 birds. The treatment groups were the negative (NC), positive (PC), egg yolk antibody powder 0.1% (T1), egg yolk antibody powder 0.5% (T2), and egg yolk antibody powder 1.0% (T3) groups. In the growth performance of ducks during the entire experimental period, the IgY groups and positive control group (PC) showed significantly higher (P<0.05) body weights and body weight gains compared to the negative control group (NC). However, no significant differences were observed in the feed intake and feed conversion ratio. The blood component profiles showed that the IgY 0.1 and 1.0% groups decreased in total cholesterol content compared to the NC group. The aspartate aminotransferase (AST) and alanine aminotranferease (ALT) contents were lower in the IgY 1.0% group, but there were no significant differences. Regrading the leukocyte content after feeding IgY, the heterophil: lymphocyte ratio decreased in the IgY groups, especially in the IgY 0.1% group, which had a lower content than the other groups. However, these results showed no significant differences. The Lactobacillus count in the intestines significantly increased (P<0.05) in the IgY 0.1 and 0.5% groups, the level of IgY increased, and the Escherichia coli count decreased. However, no significant difference was observed in the total plate count. The immunoglobulin G content was lower in the IgY groups than in the NC group, and compared with the IgY groups, the IgY 0.5% had a lower content, which was not a significant difference.

본 시험은 오리 사료 내 난황항체제제 첨가 시 생산성, 혈액 특성, 장내 미생물 및 면역글로불린에 미치는 영향을 조사하기 위하여 수행하였다. 1일령 오리(Cherry valley) 총 300수를 공시하여 5처리 3반복, 반복당 20수씩 공시하여 6주간 시험을 실시하였다. 처리구는 항생제를 첨가하지 않은 처리구(NC, negative control), 항생제 처리구(PC, positive control), 난황항체제제 0.1% 첨가구(T1), 난황항체제제 0.5% 첨가구(T2) 그리고 난황항체제제 1.0% 첨가구(T3)로 나누어 실험을 실시하였다. 종료 시 체중은 난황항체제제 첨가구와 항생제첨가구가무항생제처리구보다 유의적으로 높았고(P<0.05), 증체량 역시 난황항체제제 첨가구와 항생제 첨가구가 무항생제 처리구에 비해 높게 나타났다(P<0.05). 하지만 전 기간에 대한 사료 섭취량과 사료 요구율은 처리구간에 통계적인 유의차가 나타나지 않았다. 6주간 사양 T실험 후 혈액 생화학 및 혈구 분석 결과, 난황항체제제 0.1, 1.0% 첨가구에서는 total cholesterol 함량이 무항생제 처리구에 비해 다소 감소하는 경향을 보였으나, 통계적인 유의차는 없었다. 또한 AST와 ALT 함량 역시 처리구 간에 유의적인 차이를 보이지 않았다. 오리의 맹장 내 유산균 수는 난황항체제제 0.1와 0.5% 급여 시 다른 처리구에 비해 가장 높았으며(P<0.05), 대장균 수는 난황항체제제 수준이 증가함에 따라 감소하는 경향을 보였으나, 통계적 유의차는 없었다. 또한 혈청 내 면역글로불린 함량에도 처리구간에 유의적인 차이는 나타나지 않았다. 결과적으로 오리 사료 내 난황항체첨가 시 오리의 생산성을 개선시키고, 장내 미생물 균총에 효과적인 것으로 나타났으나, 아직까지 국내에서는 난황항체제제 첨가에 따른 사양 시험과 관련된 연구가 부족하다는 점을 감안할 때 향후 이에 대한 많은 연구가 필요할 것으로 사료된다.

Keywords

References

  1. Arasteh N, Aminirissehei AH, Yousif AN, Albright LJ, Durance TD 2004 Passive immunization of rainbow trout (Oncorhynchus mykiss) with chicken egg yolk immunoglobulins (IgY). Aquaculture 231:23-36. https://doi.org/10.1016/j.aquaculture.2003.11.004
  2. Duncan DB 1955 Multiple range and multiple F tests. Biometrics 11:1-42. https://doi.org/10.2307/3001478
  3. Facon M, Skura B, Nakai S 1993 Antibodies to a colonization factor of human enterotoxigenic E. coli in cow' milk and colostrum. Food Agric Immunol 5:85-91. https://doi.org/10.1080/09540109309354787
  4. Fulton RM, Nersessian BN, Reed WM 2002 Prevention of Salmonella enteritidis infection in commercial ducklings by oral chicken egg-derived antibody alone or in combination with probiotics. Poultry Sci 81:34-40. https://doi.org/10.1093/ps/81.1.34
  5. Girard F, Batisson I, Martinez G, Breton C, Harel J, Fairbrother JM 2006 Use of virulence factor specific egg yolkderived immunoglobulins as a promising alternative to antibiotics for prevention of attaching and effacing Escherichia coli infections. FEMS Immunol Med Microbiol 46:340-350. https://doi.org/10.1111/j.1574-695X.2005.00030.x
  6. Ibrahim El-SM, Rahman AKMS, Isoda R, Umeda K, Van Sa N, Kodama Y 2008 In vitro and in vivo effectiveness of egg yolk antibody against Candida albicans (anti-CA IgY). Vaccine 26:2073-2080. https://doi.org/10.1016/j.vaccine.2008.02.046
  7. Larsson A, Balow RM, Lindahl TL, Forsberg PO 1993 Chicken antibodies: Taking advantage of evolution-a review. Poultry Sci 72:1807-1812. https://doi.org/10.3382/ps.0721807
  8. Mahdavi AH, Rahmani HR, Nili N, Samie AH, Soleimanian-Zad S, Jahanian R 2010 Effects of dietary egg yolk antibody powder on growth performance, intestinal Escherichia coli colonization, and immunocompetence of challenged broiler chicks. Poultry Sci 89:484-494. https://doi.org/10.3382/ps.2009-00541
  9. Marquardt RR, Jin LZ, Kim JW, Fang L, Frohlich AA, Baidoo SK 1999 Passive protective effect of egg-yolk antibodies against enterotoxigenic Escherichia coli K88+ infection in neonatal and early-weaned piglets. FEMS Immun & Med Microbiology 23:283-288. https://doi.org/10.1111/j.1574-695X.1999.tb01249.x
  10. Owusu-Asiedu A, Nyachoti CM, Marquardt RR 2003 Response of early-weaned pigs to an enterotoxigenic Escherichia coli (K88) challenge when fed diets containing spray-dried porcine plasma or pea protein isolate plus egg yolk antibody, zinc oxide, fumaric acid, or antibiotic. J Anim Sci 81:1790-1798. https://doi.org/10.2527/2003.8171790x
  11. SAS 2002 SAS User's Guide. Statistics. Version 8.e, SAS Institute Inc, Cary NC.
  12. Sunwoo HH, Nakano T, Dixon WT, Sim JS 1996 Immuno response in chickens against lipopolysaccharide of Escherichia coli and Salmonella typhimurium. Poultry Sci 75:342-345. https://doi.org/10.3382/ps.0750342
  13. Van Nguyen S, Umeda K, Yokoyama H, Tohya Y, Kodama Y 2006 Passive protection of dogs against clinical disease due to canine parvovirus-2 by specific antibody from chicken egg yolk. Can J Vet Res 70:62-64.
  14. Yokoyama H, Peralta RC, Diaz R 1992 Passive protective effect of chicken egg yolk immunoglobulins against experimental enterotoxigenic Escherichia coli infection in neonatal piglets. Infect Immun 60:998-1007.
  15. Yokoyama H, Peralta RC, Umeda K, Hashi T, Icatlo Jr FC, Kuroki M, Ikemori Y, and Kodama Y 1998. Prevention of fatal salmonellosis in neonatal calves, using orally administered chicken egg yolk Salmonella-specific antibodies. Am J Vet Res 59:416-420.
  16. Kim JW, Kim DK, Kim C 2000 Production of specific egg yolk antibody against K99(F5) fimbriae from enterotoxigenic Escherichia coli. J Anim Sci & Technol (Kor) 42: 371-378.