Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Construction of a Large Synthetic Human scFv Library with Six Diversified CDRs and High Functional Diversity

  • Yang, Hye Young (Division of Life and Pharmaceutical Sciences, Ewha Womans University) ;
  • Kang, Kyung Jae (Division of Life and Pharmaceutical Sciences, Ewha Womans University) ;
  • Chung, Julia Eunyoung (Division of Life and Pharmaceutical Sciences, Ewha Womans University) ;
  • Shim, Hyunbo (Division of Life and Pharmaceutical Sciences, Ewha Womans University)
  • Received : 2008.11.07
  • Accepted : 2008.11.27
  • Published : 2009.02.28
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Abstract

Antibody phage display provides a powerful and efficient tool for the discovery and development of monoclonal antibodies for therapeutic and other applications. Antibody clones from synthetic libraries with optimized design features have several distinct advantages that include high stability, high levels of expression, and ease of downstream optimization and engineering. In this study, a fully synthetic human scFv library with six diversified CDRs was constructed by polymerase chain reaction assembly of overlapping oligonucleotides. In order to maximize the functional diversity of the library, a ${\beta}$-lactamase selection strategy was employed in which the assembled scFv gene repertoire was fused to the 5'-end of the ${\beta}$-lactamase gene, and in-frame scFv clones were enriched by carbenicillin selection. A final library with an estimated total diversity of $7.6{\times}10^9$, greater than 70% functional diversity, and diversification of all six CDRs was obtained after insertion of fully randomized CDR-H3 sequences into this proofread repertoire. The performance of the library was validated using a number of target antigens, against which multiple unique scFv sequences with dissociation constants in the nanomolar range were isolated.

Keywords

Acknowledgement

Supported by : Korea Science and Engineering Foundation, Ewha Woman's University

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