• Title/Summary/Keyword: and leaf culture

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Leaf blight of Castor Bean Plants caused by 2 Species of Phytophthora(oral)

  • Kim, B.S.;Y.S. Lim;Kim, J.H.
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.128.3-129
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    • 2003
  • A leaf blight disease caused by a species of Phytophthora has been observed on castor bean plants growing near dwelling houses in Manchon-dong, Daegu since 1993. The first isolate that we have kept was producing papillate, ovoid-obpyriform to obpyriform sporangia with on a simple sympodial sporangiophore from diseased tissue placed on water agar plates. The pure isolate, however, did not sporulate on agar media, and rarely even in water, but produced mycelial swellings and chlamydospores in water. Sporangia measured 26.1-77.4 ${\times}$ 23.2-44.0$\mu\textrm{m}$. Chlamydospores were either terminal or intercalary, and measured 24-29.4$\mu\textrm{m}$ in diameter. Sex organs were not formed in a single culture. In 2003, another pure isolate was isolated from castor bean plants with similar symptoms at the same place. The second isolate was distinct from the first one in that the second isolate was readily and abundantly sporulating on V8 juice agar plates. Sporangia of the second isolate were papillate, ovoid and caduceus with a pedicel. Sporangia measured 19.5-48.8 x 17.6-34.3$\mu\textrm{m}$ with 3.7$\mu\textrm{m}$ high papilla and 4.1$\mu\textrm{m}$ long pedicel. No sex organs were formed in a single isolate culture. Both isolates were pathogenic on castor bean plants. Results of the efforts to identify the two species of Phytophthora will be discussed.

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Effect of Medium Component on Plant Regeneration via Adventitious Bud Formation from Leaf Explant Cultures of Strawberry (Fragaria ananassa Duch.) (딸기 (Fragaria ananassa Duch.) 잎 절편체 배양으로부터 부정아 형성을 통한 식물체 재생)

  • 조덕이;소웅영;정원일
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.3
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    • pp.171-178
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    • 2001
  • This study was investigated to establish a regeneration system of plant via adventitious bud formation from leaf explant cultures of strawberry (Fragaria ananassa Duch). Effects of plant growth regulators (2,4-D, BAP), agar sucrose and myo-inositol on adventitious bud formation were investigated. When the leaf explants were cultured on MS medium supplemented with 0.1 mg/L 2,4-D and 3 mg/L BAP, the adventitious bud formation was most promoted. The adventitious bud formation was not induced from leaf explants cultured on MS medium containing 2,4-D alone. Adventitious bud formation was enhanced to almost 3 times on medium with low level of agar concentration (0.4%) in comparison with those on the medium with high level of agar (1%), but almost of shoot was vitrificated on the medium. Therefore, the normal adventitious bud formation from leaf explants was most effective on the medium containing 0.05 mg/L 2,4-D,1 mg/L BAP, 0.8% agar, 30 g/L sucrose and 100 mg/L myo-inositol. Thus, the mass propagation of healthy strawberry could be established using leaf explants.

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Effect of Goldenrod (Solidago virgaurea) Leaf and Stem Powder on Physical and Sensory Characteristics of Emulsion-type Sausages

  • Choe, Ju-Hui;Kim, Hack-Youn;Han, Doo-Jeong;Kim, Yong-Jae;Park, Jae-Hyun;Ham, Youn-Kyung;Kim, Cheon-Jei
    • Food Science of Animal Resources
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    • v.31 no.5
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    • pp.668-675
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    • 2011
  • The effects of adding goldenrod leaf (GL) and stem powder (GS) (1 and 2%) to sausage meat were evaluated for quality characteristics. The compositional properties, pH, cooking yield, instrumental color, emulsion stability, viscosity, texture, and sensory properties were investigated. Adding GL and GS increased the moisture and ash concentration in sausages. Furthermore, increasing the amount of added GL and GS helped to develop (p<0.05) cooking yield, emulsion stability, and viscosity in the meat batters. Sausages with 2% GL and GS had significantly higher springiness and hardness than those of the control. No significant differences in cohesiveness were observed among the treatments. CIE L*and CIE a*values were highest (p<0.05) in the control sausage. Treatment with 2% GL resulted in the highest CIE b* values (p<0.05) in batter and sausages. No significant difference was observed between the control and other treatments for the overall acceptability. Overall, this study indicated that GL and GS could be used as dietary fiber in sausages to improve quality characteristics.

Chemical Composition of Pine Sprouts and Pine Needles for the Production of Pine Sprout Tea (송순차 제조를 위한 송순 및 솔잎의 화학적 조성)

  • Chung, Hee-Jong;Hwang, Geum-Hee;Yoo, Maeng-Ja;Rhee, Soon-Ja
    • Journal of the Korean Society of Food Culture
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    • v.11 no.5
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    • pp.635-641
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    • 1996
  • As a basic study for preparing pine sprout tea, chemical components in pine sprouts and pine needles were analyzed as follows: In proximate composition the contents of most components except for crude fat were different between in pine sprouts and pine needles. Moisture content in pine sprouts was higher than that in pine needles. Calcium and potassium were major minerals contained in pine sprouts and needles, and their contents in pine needles picked in December were higher than those picked in June. Soluble tannin and vitamin C contents in leaf part of pine sprouts were much higher than those in stem part and their contents in pine needles were increased according to their growth. Free sugars like fructose, glucose and sucrose were contained in both pine sprouts and needles, and their contents in pine sprouts were higher in stem part as compared that in leaf part. Although fourteen kinds of amino acids were detected in pine sprouts and pine needles, their contents were extremely low. Amino acid composition between pine sprouts and pine needles was different each other, but major amino acids contained in them were same, those are acidic amino acids such as aspartic acid and glutamic acid. Amino acid contents in pine needles were increased according to the growth. In fatty acid composition in leaf part of pine sprouts, saturated fatty acid contents were higher than unsaturated fatty acid contents, but in stem part unsaturated fatty acid contents were higher. In pine needles the amount of saturated fatty acid was increased with the growth, but the amount of unsaturated fatty acid was rather decreased.

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Influences by position of node and existence of leaf on microtuberization in node culture of potato (감자절간배양에서 마디위치 및 잎의 부착유무에 따른 소괴경형성의 차이)

  • Hwang, Hye-Yeon;Lee, Young-Bok
    • Journal of Plant Biotechnology
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    • v.35 no.1
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    • pp.63-68
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    • 2008
  • Single-node stem pieces ca. 1 cm in length containing a axillary bud were obtained from in vitro plants of potato (Solanum tuberosum L.). The influences by a position of the node and the existence of a leaf at the node were observed in the single-node culture on the 8% sucrose MS medium. The effect of CCC was also investigated for the microtuberization. The apical part node was excellent in the tuberization not to mention shoot length, fresh weight, diameter, the number of node on the in vitro culture of a single-node than the lower part. The differences in the diameter of a tuber formed in the part of the axillary bud on all treatments including the cultivation of the apical part node were not recognized. However, the fresh weight of the tuber showed high value in the tuber formed at the axillary bud of shoot apex part. At 20 days after cultivation, tuberization was promoted in the new stolen that developed from the bud of node with a leaf under SD condition of 8 hours at $20^{\circ}C$. The tuberization from axillary bud of the single-node without leaf was inhibited at high temperature of $28^{\circ}C$ regardless of daylength. Whereas, tuberization at $20^{\circ}C$ and $28^{\circ}C$ was similar without the difference under SD condition but the tuber formation ratio were low. CCC 500 mg/L promoted tuberization and the effect was also showed even under LD condition at $28^{\circ}C$. The inhibiton of tuberization under LD and high temperature condition could be solved by treatment with CCC.

Studies on the Effects of Organic Fertilizers on the Growth and Yield of Radish (Raphanus sativus L.) (유기질비료 시용이 무의 생육 및 수량에 미치는 영향에 관한 연구)

  • Kim, Cheol-Ho;Yun, Hwa-Mo
    • The Journal of Natural Sciences
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    • v.5 no.1
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    • pp.29-36
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    • 1992
  • The experiment was conducted to evaluate the effects of organic fertilizer. Sarang of fermented poultry manure. Miwon glutamic acid fertilizer and poultry manure, on the growth and yield of radish cultivated in spring and autumn. 1) The growth of leaf length, leaf weight, root length, root diameter and root weight of radish in two culture types was stimulated in order of NPK, fermented poultry manure + 1/5NK, fermented poultry manure, poultry manure and control treatment, and chlorophyll and nitrogen content of leaf were increased in order of the above mentioned fertilizers.2) Yield decreased in fermented poultry manure plot of 450kg application comparison with NPK plot but the one of 1800kg fermented poultry manure was increased. The yield of 450kg Sarang plot was sufficient as compared with Korean average yield of spring and autumn culture.3) The difference of growth of radish between treatments of fermented poultry manure and NPK was lowered by continuous application of Sarang organic fertilizer.4) Soil nitrogen content and exchangeable Mg were similar in all treatments but soil pH, the contents of organic matter, $P_2 O_5$ and Ca were high in the fermented poultry manure treatment.

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Analyses of Transpiration and Growth of Paprika (Capsicum annuum L.) as Affected by Moisture Content of Growing Medium in Rockwool Culture

  • Tai, Nguyen Huy;Park, Jong Seok;Shin, Jong Hwa;Ahn, Tae In;Son, Jung Eek
    • Horticultural Science & Technology
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    • v.32 no.3
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    • pp.340-345
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    • 2014
  • Since the moisture content (MC) of growing medium closely related with the crop transpiration, the MC should be included to the environmental factors to be considered for irrigation control in soilless culture. The objective of this study was to analyze the transpiration of paprika plants using daily mean solar radiation (RAD) and vapor pressure deficit (VPD) as well as the growth of the plants at different MCs of rockwool growing media. The starting points of irrigation were controlled by a moisture sensor with minimum set points of 40%, 50%, and 60% of MCs. The canopy transpirations were measured for 80 to 120 days after transplanting and analyzed. The transpirations were well regressed with a combination of both RAD and VPD rather than daily mean RAD only under the controlled MCs. The transpiration at 60% MC was higher than those at 50% and 40% MCs. Leaf area, leaf fresh and dry weights at 60% MC were higher than those at 50% and 40% MCs while the number of leaves had no significant difference among the MCs. There were no significant differences in number of fruits and fruit size among all the MCs, while fruit weight was significantly lower at 40% MC than other treatments. Fresh and dry fruit yields were the highest at 60% MC. Therefore it was concluded that the transpiration was affected by the MC of rockwool growing medium and the minimum set point of 50-60% MC of rockwool growing medium gave better effects on the growth of the paprika plants.

Occurrence of Leaf Spot on Eriobotrya japonica Caused by Entomosporium mespili in Korea (Entomosporium mespili에 의한 비파나무 점무늬병 발생)

  • Seo, Sang-Tae;Park, Mi-Jeong;Shin, Hyeon-Dong;Kim, Kyung-Hee
    • Research in Plant Disease
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    • v.17 no.2
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    • pp.228-231
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    • 2011
  • Since 2008, a new leaf spot disease has been found in loquat tree (Eriobotrya japonica) at a nursery station in Goheung, Korea. Above 50% of the seedlings developed the spot symptoms on the leaves. The infected plants were generally reduced in the seedling vigour and early defoliation was occurred. The infected leaves developed grayish lesion with dark reddish brown margin. The pathogen developed round to ellipsoidal acervuli on the lesion. Based on morphological characteristics of the pathogen, the fungus was identified as Entomosporium mespili. The cultured pathogen successfully reproduced the same disease symptom on the leaves of loquat tree and found to be E. mespili. A monoconidial culture was deposited in Korean Agricultural Culture Collection (KACC 44727). This is the first report of E. mespili causing leaf spot of loquat tree in Korea.

Growth of Non-Powered Hydroponics Equipment and Quality Characteristics according to Post-Harvest Packaging by Cultural Methods on Leaf Lettuce (무동력 수경재배 장치의 상추 생육과 수확 후 포장에 따른 품질 특성)

  • Jung-Soo, Lee
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.28 no.3
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    • pp.231-236
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    • 2022
  • The research was aimed to improve pre-harvest methods to maintain marketability in postharvest leaf lettuce. In this study, the effect of hydroponics on the growth and post-harvest storage characteristics of 'Chongchima' lettuce grown in peat mass medium hydroponic system using a non-powered culture device or deep water culture (DWC) was evaluated. There was no difference in fresh weight, leaf number, SPAD, moisture content, and C/N ratio between peat moss growing medium hydroponic and DWC methods except plant height. It was found that lettuce cultivation by a nonpowered hydroponics method is easier than the existing DWC. The quality parameters, such as fresh weight loss, SPAD value, and general appearance of lettuce were investigated after harvest. There was no significant difference in fresh weight loss and general appearance during storage of lettuce by the hydroponics methods. However, with the increased storage time of SPAD, which is related to chlorophyll content, was slightly higher in peat moss medium hydroponic was than DWC. When crops such as lettuce are grown under favorable conditions without any agronomic abnormalities, it is suggested that post-harvest storage is not significantly affected by peat moss growing medium hydroponic and DWC.