• Analytical Science and Technology
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• v.8 no.4
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• pp.859-864
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• 1995

Mono- and oligosaccharides are derivatized with p-aminobenzoic ethyl ester (ABEE), strongly absorbs UV light at 254 nm, in the presence of sodium cyanoborohydride. C18-bonded silica column is used for the separation of sugar-ABEE derivatives in an isocratic mode. RP-HPLC conditions are optimized by using ternary mixture as mobile phase and $45^{\circ}C$ as a column temperature. Sugar-ABEE derivatives are separated well within a short run time (ca. 25 min) by reverse-phase partition chromatographic mode. The ($1{\rightarrow}6$) linkage type of dihexose-ABEE derivatives has shorter retention time than ($1{\rightarrow}4$)-linkage type. After acid hydrolysis of glycoproteins with 2M trifluoroacetic acid, monosaccharide composition and contents are determined. This procedure is very useful for the simultaneous analysis of neutral and amino sugars in a single chromatographic step using RP-HPLC without reacetylation of deacetylated amino sugars, which are produced by acid hydrolysis.

• Steel and Composite Structures
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• v.32 no.5
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• pp.643-655
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• 2019

Perforation and cutouts of structures are compulsory in some modern applications such as in heat exchangers, nuclear power plants, filtration and microeletromicanical system (MEMS). This perforation complicates dynamic analyses of these structures. Thus, this work tends to introduce semi-analytical model capable of investigating the dynamic performance of perforated beam structure under free and forced conditions, for the first time. Closed forms for the equivalent geometrical and material characteristics of the regular square perforated beam regular square, are presented. The governing dynamical equation of motion is derived based on Euler-Bernoulli kinematic displacement. Closed forms for resonant frequencies, corresponding Eigen-mode functions and forced vibration time responses are derived. The proposed analytical procedure is proved and compared with both analytical and numerical analyses and good agreement is noticed. Parametric studies are conducted to illustrate effects of filling ratio and the number of holes on the free vibration characteristic, and forced vibration response of perforated beams. The obtained results are supportive in mechanical design of large devices and small systems (MEMS) based on perforated structure.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.17 no.1
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• pp.83-91
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• 2004

Nonlinear system responses of an impact system under randomly perturbed harmonic excitations are predicted in the probability domain by adopting the semi-analytical procedure previously developed. The semi-analytical procedure is obtained by solving the Fokker-Planck equation corresponding to the stochastic differential equation of the given impact system by utilizing the path-integral solution. The evolutionary joint probability density functions are generated by using the method, and the characteristics of nonlinear dynamic response behaviors of the system are examined. Noise effects on the responses are also examined. It Is found that the semi-analytical method can provides the accurate information of the responses via the joint probability functions for the impact system. It is found that the noises weaken and eventually terminate the chaos in the responses, but it is also found that the chaotic signatures reside in the presence of the external noise with relatively high intensity. The joint probability density function shows that the ensemble of the system responses are weakly stationary.

• Journal of Ginseng Research
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• v.45 no.5
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• pp.539-545
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• 2021

Background: Red ginseng polysaccharides (RGPs) have been acknowledged for their outstanding immunomodulation and anti-tumor activities. However, their studies are still limited by the complexity of their structural features, the absence of purification and enrichment methods, and the rarity of the analytical instruments that apply to the analysis of such macromolecules. Thus, this study is an attempt to establish a new mass spectrometry (MS)-based analysis procedure for RGPs. Methods: Saponin pre-excluded powder of RG (RG-SPEP, 10 mg) was treated with 200 µL of distilled water and centrifuged for 5 h at 1000 rpm and 85 ℃. Ethanol-based precipitation and centrifugation were applied to obtain RGPs from the heated extracts. Further, endo-carbohydrase treatments were performed to produce specific saccharide fragments. Solid-phase extraction (SPE) processes were implemented to purify and enrich the enzyme-treated RGPs, while matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) MS was employed for the partial structural analysis of the obtained RGPs. Results: Utilizing cellulase, porous graphitized carbon (PGC), hydrophilic interaction chromatography (HILIC), and MALDI-TOF/TOF MS, the neutral and acidic RGPs were qualitatively analyzed. Hex_n and Hex_n-18 (cellulose analogs) were determined to be novel neutral RGPs. Additionally, the [Unknown + Hex_n] species were also determined as new acidic RGPs. Furthermore, HexA_n (H) was determined as another form of the acidic RGPs. Conclusion: Compared to the previous methods of analysis, these unprecedented applications of HILIC-SPE and MALDI-TOF/TOF MS to analyze RGPs proved to be fairly effective for fractionating and detecting neutral and acidic components. This new procedure exhibits great potential as a specific tool for searching and determining various polysaccharides in many herbal medicines.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.10a
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• pp.233-238
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• 2000

The purpose of this study is to outline the analysis procedure for evaluating the performance of moment resisting steel frames. For this purpose, three ordinary moment resisting frames are designed in compliance to UBC 1994. The evaluation is performed by nonlinear static procedures using two analytical models. Only one analytical model using panel element can reflect the panel zone deformation explicitly. The limit values in FEMA 273 are used as guidelines of predicted demand parameters by which the performance of OMRFs may be assessed.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1991.10a
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• pp.63-68
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• 1991

The frequency window method has been extended to include strong coupling and multiple connections between the primary structure and the secondary structures. The rational polynomial expansion of the eigenvalue problem and the analytical methods for its solution are novel and distinguish this work from other eigenvalue analysis methods. The key results are the identification of parameters which quantify the resonance and coupling characteristics; the derivation of analytical dosed-form expressions describing the fundamental modal properties of the frequency windows; and the development of an iterative procedure which yields accurate convergent results for strongly-coupled primary-secondary structures.

• Water for future
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• v.8 no.2
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• pp.93-99
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• 1975

A linear and optimum linear systems have been reviewed in some detail. The procedure of the solution of the Wiener-Hopf equation analytically in time domain is given and the prediction of downstream outflow for given upstream inflow are made. The predicted results are fairly satisfaotory. The intended physical interpretation of the analytical solution could be descriptable but it was found that the evaluation of the parameters of the response function is rather difficult due to complicacy and a great deal of works.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1995.10a
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• pp.859-863
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• 1995

Presented in the paper are a review of sorting and buffering system(SBS), the design procedure of a SBS and an analytical model for the initial alternative generation. SBS is an automated material handling system in which incoming items of different part types are automatically sorted and buffered so that the processing machines can process the part in lots. SBSs play a key role in modern manufacturing systems ans are widely found in mass fabrication lines, packaging and palletizing, and disribution centers.

• Proceedings of the Korean Geotechical Society Conference
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• 1994.09a
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• pp.119-122
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• 1994

An analytical procedure is described to estimate the mobilized tensile forces along the effective lengths of nails. Based on the horizontal focing displacements of a nailed-soil wall experiencing outward tilt about the toe with granular soil deposit, the variation of nail-soil friction coefficient is modeled. Also, the method of overall stability analysis of a nailed-soil wall is presented using the Morgenstem-Price limit-equilibrium slice method. The results predicted by the developed procedure are compared with test measurements. The comparisons show in general good agreement.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1999.10a
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• pp.61-68
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• 1999

This paper describes the method of evaluating the elastic modulus of soil medium by using the Circular Plate Loading Test. The elastic foundaton is considered to be the elastic half-space. The stiffness matrix of elastic half space is drived using Boussinesq's analytical soulution. A numerical examples are presented to verify the validity of this procedure. Also, the numerical results are compared with others by the existing study results. The procedure proposed in this theses can be applied to the design of paving concrete resting on the elastic foundation.