• Journal of Navigation and Port Research
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• v.29 no.3 s.99
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• pp.275-282
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• 2005

The year 2003 is the centennial of the birth of the modem lighthouse in Korea the lighthouse was originally surrounded by a beautiful nature and it contains the romantic sensitivity which relates with the ocean. And in some case it is a environment-friendly architectural facility which uses a alternative energy like a solar cell. Now the properly developed manned lighthouse is not only functioning as a traditional aids to navigation for the vessel, but also it becomes the new facility and space for all civilians who might visit and experience. If we want to vitalize the traditional lighthouse into water-familiar space, we must reconsider the facilities, various operational programs and the institutions for future-oriented development. For this situation we intended to reveal in this study that all the issues which relate to the proper use of the manned lighthouses in the whole country, since the project of water-familiar space started by MOMAF.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.2
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• pp.147-153
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• 2013

To prevent paralytic shellfish poisoning (PSP) due to the consumption of shellfish contaminated with PSP toxins, the quantitative analysis of these toxins is very crucial. The AOAC International mouse bioassay (MBA) has been used widely for the routine monitoring of PSP toxins for more than 50 years. However, this method has low sensitivity and high limit of quantification (LOQ) and interferences from other components in the extract, and it cannot determine toxic profiles. Ethical problems also exist with the continued use of this live mouse assay. To establish an alternative method to the MBA used for PSP toxins analysis, we attempted to optimize the analysis conditions of a precolumn high-performance liquid chromatography (HPLC) oxidation method and succeeded in validating its accuracy and precision in quantifying PSP toxins. A clear peak and the isolation of PSP toxins were obtained by injecting the working standards of Certified Reference Materials using HPLC. The LOQ of the precolumn HPLC oxidation method for PSP toxins was about $0.1002{\mu}g/g$, which represented an approximately fourfold improvement in detection capability versus the AOAC MBA. The intra-accuracy and precision for PSP toxins in oysters were 77.0-103.3% and 2.0-5.7%, respectively, while the respective inter-accuracy and precision were 77.3-100.7% and 2.4-6.0%. The mean recoveries of PSP toxins from oysters were 75.2-112.1%. The results of a comparison study showed good correlation between the results of the precolumn HPLC oxidation method and those of MBA, with a correlation factor of 0.9291 for mussels. The precolumn HPLC oxidation method may be used as an alternative to, or supplementary method with, MBA to monitor the occurrence of PSP toxins and to analyze the profiles of these toxins in shellfish.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.29 no.1
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• pp.21-29
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• 2005

The year 2003 is the centennial of the birth of the modern lighthouse in Korea. The lighthouse was originally surrounded by a beautiful nature and it contains the romantic sensitivity which relates with the ocean. And in some case it is a environment-friendly architectural facility which uses a alternative energy like a solar cell. Now the properly developed manned lighthouse is not only functioning as a traditional aids to navigation for the vessel, but also it becomes the new facility and space for all civilians who might visit and experience. Jeju regional maritime affairs & fisheries office has developed the manned lighthouses properly to ocean cultural space for the past several years. As a result of that, the recognition regarded on ocean culture was raised and the regional tourism was vitalized. For this situation we intended to reveal in this study that all the issues which relate to the proper use of the manned lighthouse in the Jejudo, since the project of water-familiar space started by MOMAF.

• Journal of Environmental Impact Assessment
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• v.7 no.2
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• pp.89-102
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• 1998

The purpose of this study is to seek AEI(Air Environmental Indices), PSI(Pollutant Standard Index) and the urban air quality control goal(the best available alternative energy program) by assessing the best ratio of energy types used in urban enterprises, based on harmful health effect and air quality standard and costs. This study is focused on an urban area(Puchun), where area sourcees are associated with heavy traffic, large population, and its industrial sources with large emissions. In the first step, air modeling, estimation of AEI and PSI, and benefit-cost analysis were carried out. In the second step, we assessed that 660 scenarios about the ratio of B-C oil, light oil and LNG used in urban enterprises with regard to air quality and cost. In the third step, the best available alternative energy program was selected for the ratio of energy species(B-C oil, light oil and LNG) by using the lexicographic method. From the emission analysis, main source of $NO_2$ is identified as industries and air quality is evaluated according to the ratio of B-C oil, light oil and LNG used in urban enterprise. The modeling data of TSP, $SO_2$, $NO_2$, CO, $O_3$, by ISC3 and PBM are respectively $118{\mu}g/m^3$, 0.027ppm, 0.025ppm, 2.0ppm, 0.55ppm in indurstrial area. That data are close to Environmental Air Quality Standard. By means of sensitivity analysis, we obtained the difference in concentration between the areas(Nae-dong, Joong-dong) according to the ratio of B-C oil, light oil and LNG used in the industries. From the result of alternatives assessment the lowest AEI value and cost, the ratio of B-C oil, light oil and LNG are 2.5%, 20%, 77.5%, respectively.

• The Korean Journal of Cytopathology
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• v.18 no.1
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• pp.29-35
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• 2007

This study purposed to evaluate a $CellPrep^{(R)}$ (CP) of liquid-based cytology (LBC) to search for a less expensive and automated alternative cytologic preparation technique applicable to usually encountered cytologic specimens. Cervicovaginal direct-to-vial split samples from 457 gynecologic patients, 40 body fluid samples, and 34 urine samples were processed with the CP technique and the results were compared with those of currently used $ThinPrep^{(R)}$ (TP) method. Both CP and TP methods provide evenly distributed thin layers of cells with little cellular overlaps or significant obscuring elements in most of cases. Staining quality of both preparations showed a little difference due to the difference of fixative solutions without significant distractions in cytologic interpretation. On the supposition that TP was a gold standard, sensitivity, specificity, positive predictive value, and negative predictive value of CP cytology were 89%, 98%, 86%, and 99% in the cervicovaginal smear, 89%, 82%, 80%, and 90% in body fluid, and all of these values were 100% in urine samples. To testify the availability of immunohistochemistry on CP preparations, cytokeratin, vimentin, and Ki-67 were applied on body fluid specimens, and all of these antibodies were specifically stained on targeted cells. Conclusively, the CP method gave comparable results to those of TP in terms of smear quality and cytologic diagnostic evaluation, and was available on immunohistochemistry. The CP method could offer a cost-effective and automated alternative to the current expensive techniques of liquid-based cytology on popular cytologic materials including cervicovaginal, body fluid, and urine specimens.

• Journal of the Korean Institute of Intelligent Systems
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• v.18 no.1
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• pp.139-144
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• 2008

Generally, real system that is the object of decision-making is very variable and sometimes it lies situations with uncertainty. To solve these problem, it has used statistical methods as significance level, certainty factor, sensitivity analysis and so on. In this paper, we propose a method for fuzzy decision-making based on MFAC(Multiple Fuzzy Alpha Cut) to improve the definiteness of classification results with similarity evaluation. In the proposed method, MFAC is used for extracting multiple a ${\alpha}$-level with proximity degree at proximity relation between relative Hamming distance and max-min method and for minimizing the number of data which are associated with the partition intervals extracted by MFAC. To determine final alternative of decision-making, we compute the weighted value between extracted data by MFAC From the experimental results, we can see the fact that the proposed method is simpler and more definite than classification performance of the conventional methods and determines an alternative efficiently for decision-maker by testing significance of sample data through statistical method.

• Journal of Embryo Transfer
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• v.24 no.4
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• pp.275-279
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• 2009

The beneficial effect of glycerol as a cryoprotectant, especially for sperm cryopreservation, has been shown in many studies. However, glycerol is toxic to living cells, and boar sperm in particular show greater sensitivity to glycerol than sperm from other domestic animals. Amides have been studied as alternative cryoprotectants for freezing stallion sperm. Sperm frozen in methylformamide or dimethylformamide as cryoprotectants show similar motility when thawed compared with sperm frozen in glycerol. We evaluated the cryoprotective effects of dimethylformamide on boar sperm freezing. To test the effect of amides, the concentration of boar semen was adjusted to $10^9sperm/mL$, and seminal plasma was removed using Hulsen solution. After centrifugation, the pellet was diluted in modified-Modena B extender. Lactose-egg yolk (LEY) extender was used as the cooling extender. The freezing extender was madeed aaddition of the optimal amount of glycerol and amides to LEY-Glycerol-Orvus ES Paste extender, and this extender was used for the second dilution. Diluted sperm were frozen in liquid nitrogen using the 0.5 mL straw method. Sperm frozen in extender with glycerol as a cderol were compared with those frozen in extender including the different amides. Sperm were tested for motility, viability, the sperm chromatin structure assay, and normal apical ridge after thawing. The percent of motile sperm diluted in glycerol was as high as that in the stallion study (61%). Dimethylformamide showed positive effects on sperm quality and was better than glycerol. Methylformamide provided similar sperm quality as glycerol. Therefore, dimethylformamide is useful for reducing cryoinjury in boar sperm and is expected to be useful as an alternative cryoprotectant.

• Parasites, Hosts and Diseases
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• v.60 no.1
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• pp.25-34
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• 2022

Alveolar echinococcosis (AE) caused by infection with E. multilocularis metacestode, represents one of the most fatal helminthic diseases. AE is principally manifested with infiltrative, proliferating hepatic mass, resembling primary hepatocellular carcinoma. Sometimes metastatic lesions are found in nearby or remote tissue. AE diagnosis largely depends on imaging studies, but atypical findings of imaging features frequently require differential diagnosis from other hepatic lesions. Serological tests may provide further evidence, while obtaining reliable AE materials is not easy. In this study, alternative antigens, specific to AE were identified by analyzing E. granulosus protoscolex proteins. An immunoblot analysis of E. granulosus protoscolex showed that a group of low-molecular-weight proteins in the range from 14 kDa to 16 kDa exhibited a sensitive and specific immune response to AE patient sera. Partial purification and proteomic analysis indicated that this protein group contained myosin, tubulin polymerization promoting protein, fatty-acid binding protein, uncharacterized DM9, heat shock protein 90 cochaperone tebp P-23, and antigen S. When the serological applicability of recombinant forms of these proteins was assessed using enzyme-linked immunosorbent assay, DM9 protein (rEgDM9) showed 90.1% sensitivity (73/81 sera tested) and 94.5% specificity (172/181 sera tested), respectively. rEgDM9 showed weak cross-reactions with patient sera from the transitional and chronic stages of cystic echinococcosis (3 to 5 stages). rEgDM9 would serve as a useful alternative antigen for serodiagnosis of both early- and advanced-stage AE cases.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.12
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• pp.5147-5152
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• 2016

Background: Investigations of methods for detection of mutations have uncovered major weaknesses of direct sequencing and pyrosequencing, with their high costs and low sensitivity in screening for both known and unknown mutations. High resolution melting (HRM) analysis is an alternative tool for the rapid detection of mutations. Here we describe the accuracy of HRM in screening for KRAS and BRAF mutations in metastatic colorectal cancer (mCRCs) samples. Materials and Methods: A total of 1000 mCRC patients in Mehr Hospital, Tehran, Iran, from Feb 2008 to May 2012 were examined for KRAS mutations and 242 of them were selected for further assessment of BRAF mutations by HRM analysis. In order to calculate the sensitivity and specificity, HRM results were checked by pyrosequencing as the golden standard and Dxs Therascreen as a further method. Results: In the total of 1,000 participants, there were 664 (66.4%) with wild type and 336 (33.6%) with mutant codons 12 and/or 13 of the KRAS gene. Among 242 samples randomly checked for the BRAF gene, all were wild type by HRM. Pyrosequencing and Dxs Therascreen results were in line with those of the HRM. In this regard, the sensitivity and specificity of HRM were evaluated as 100%. Conclusion: The findings suggest that the HRM, in comparison with DNA sequencing, is a more appropriate method for precise scanning of KRAS and BRAF mutations. It is also possible to state that HRM may be an attractive technique for the detection of known or unknown somatic mutations in other genes.

• Fire Science and Engineering
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• v.31 no.2
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• pp.52-60
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• 2017

Numerical simulations were performed to evaluate the habitability of an operator for a cabinet fire in the main control room of a nuclear power plant presented in NUREG-1934. To this end, a Fire Dynamics Simulator (FDS), as a representative fire model, was used. As the criteria for determining the habitability of operator, toxic products, such as CO, were also considered, as well as radiative heat flux, upper layer temperature, smoke layer height, and optical density of smoke. As a result, the probabilities of exceeding the criteria for habitability were evaluated through the sensitivity analysis of the major input parameters and the uncertainty analysis of fire model for various fire scenarios, based on V&V (Verification and Validation). Sensitivity analyses of the maximum heat release rate, CO and soot yields, showed that the habitable time and the limit criterion, which determined the habitability, could be changed. The present methodology will be a realistic alternative to enhancing the reliability for a habitability evaluation in the main control room using uncertain information of cabinet fires.