• 사상체질의학회지
• /
• 제11권2호
• /
• pp.283-299
• /
• 1999

1. 연구목적 kainic acid를 실험동물에 주입할 경우 간질발작을 일으키고 변연계 특히 해마체 부위에서 조직의 손상이 일어나게 되는데 이는 사람에 있어 측두엽성 간질에서 보이는 구조적 변화와 유사한 것이다. 본 실험은 신경독성 물질인 kainic acid로 마우스의 해마체에 손상을 유발하고 이 경우에 열다한소탕이 신경보호효과가 있는지의 여부를 알고자한 것이다. 2. 연구방법 kainic acid를 경구투여하고 열다한소탕을 3주일간 복용시키면서 각각 1, 2, 3일과 1주, 3주에 조직을 관찰하였다. 조직손상은 해마체의 CA1, CA3과 thalamus, amygdala 등에서 c-fos 와 DNA fragmentation의 출현 율로 지표를 삼았으며 광학현미경하에서 육안적 관찰을 병행하였다. 3. 결과 및 결론 kainic acid만을 투여한 대조 군에서는 실험 3주째에도 손상지표인 c-fos 와 DNA fragmentation이 발견되었으나 열다한소탕을 투여한 실험 군에서는 실험 약 3일 째부터 손상지표의 발현이 줄어 7일째에는 나타나지 않음을 알 수 있었다. 또한 현미경 하의 육안적 관찰에서도 실험초기 많은 손상을 보였던 신경세포가 2주일 후 어느 정도 회복되는 것을 볼 수 있었다. 이로써 열다한소탕이 kainic acid로 유발된 실험동물의 해마체손상에 대하여 신경세포 보호 효과 및 손상 억제 효과가 있음을 알 수 있었다.

• 사상체질의학회지
• /
• 제34권2호
• /
• pp.75-83
• /
• 2022

Objectives The aim of this study is to report the improvement in Taeeumin's dryness-heat symptom of a 54-year-old man diagnosed with type-II diabetes mellitus after treated by herbal medicine. Methods The Taeeumin patient took Yuldahansotang-gamibang for 9 months to manage Taeeumin's dryness-heat symptom. We examined the changes of Body Mass Index(BMI), Fating Blood Sugar(FBS), Hemoglobin A1c(HbA1c), and ketouria. Results Type-II diabetes mellitus symptoms in the patient with Taeeumin's dryness-heat symptom were thirst, fatigue, and increased body weight. All numerical levels for blood sugar, HbA1c, and ketouria were reduced after taking Yuldahansotang-gamibang. Conclusions The treatment of the patient with Taeeumin's dryness-heat symptom and type-II diabetes mellitus with Yuldahansotang-gamibang was efficient, and further research is needed to prove the effect of Yuldahansotang-gamibang for treating such patients.

• 사상체질의학회지
• /
• 제10권2호
• /
• pp.565-588
• /
• 1998

1. 연구배경 및 목적 태음인(太陰人) 처방(處方) 중 열다한소탕(熱多寒少楊)과 조위속명탕(調胃續命湯)이 고혈압(高血壓)과 신장기능(腎臟機能)에 미치는 효능을 실험적으로 규명하고 이들 질환에 대한 사상의학적(四象醫學的) 접근(接近)을 도모하고자 한다. 2. 방법 정상백서(正常白鼠)와 자연발증(自然發證) 고혈압(高血壓) 백서(白鼠) 두 집단을 같은 환경에 2주 이상 적응시킨 후 각각의 집단에 10첩 분량의 열다한소탕(熱多寒少湯)과 조위속명탕(調胃續命湯) 전탕액(煎湯液)을 투여하였다. 약물 투여 후 일정한 간격을 두고 혈압(血壓)을 측정하고 소사(小使)중 creatinine, electrolytes, osmolarity, renin, atrial natriuretic peptide와 혈액(血液)중 aldosterone, triglyceride, phospholipid, cholesterol을 측정하여 이들 수치들의 유의성있는 변화를 관찰하였다. 3. 결과 열다한소탕(熱多寒少湯)과 조위속명탕(調胃續命湯)은 자연발증(自然發證) 고혈압(高血壓) 백서(白鼠)의 혈압(血壓) 상승(上升)을 억제하였으며, 이 중 열다한소양(熱多寒少楊)은 혈장 atrial natriuretic peptide 농도 증가 및 aldosterone 농도 감소에 의한 것이며, 조위속명탕(調胃續命湯)은 혈장 aldosterone 농도 감소에 의한 것으로 볼 수 있어 열다한소탕(熱多寒少湯)과 조위속명탕(調胃續命湯)의 차이를 관찰할 수 있었다.

• 사상체질의학회지
• /
• 제14권1호
• /
• pp.79-89
• /
• 2002

To evaluate the effect of Yuldahansotang(YHT) water extract on cultured hippocampal cell was inhibited by hydrogen peroxide, MTT assay, NR assay, Neurofilament enzymeimmuno assay and DNA synthesis assay were carried out after the cultured hippocampal cells were preincubated with various concentrations of YHT water extract for 3 hours prior to exposure of hydrogen peroxide. The results obtained were as follows: 1. Hydrogen Peroxide decreased the survival rate of the cultured hippocampal cells on NR assay and MIT assay. 2. YHT water extract have efficacy of increasing a amount of neurofilament decreased by hydrogen peroxide in cultured hippocampal cells. 3. YHT water extract have efficacy of increasing DNA synthesis decreased by hydrogen peroxide in cultured hippocampal cells. From above the results, It is concluded that YHT has marked efficacy in preventing for the damages by hydrogen peroxide.

• 사상체질의학회지
• /
• 제16권1호
• /
• pp.148-154
• /
• 2004

Abdominal distention is a common condition, which usually results from over-eating, rather than from a serious illness. Abdominal distention is often caused by intestinal gas. Abdominal distention may also occasionally result from the accumulation of fluid in the abdomen, which can be a sign of serious medical problems. This stroke patient has been abdominal distention without medical problems. So he was treated by Yuldahansotang(熱多寒少湯) and was improved not only activity daily life but also Abdominal distention.

• 사상체질의학회지
• /
• 제14권2호
• /
• pp.169-174
• /
• 2002

A 55-year-old woman who was diagnosed as Diabetic foot was admitted to Dong-so Oriental-medical hospital on April 15th, 2002. Her great toe and secondary were to be tut off, and she was much depressed. We diagnosed her constitution as Taeumin(太陰人), and classified her symptom as Taeumin-Ban-Chang-Byung( 太陰人 ?瘡病 ) and prescribed Yuldahansotang(熱多寒少湯) for the purpose of chengganjoyoil(淸肝燥熱). During admission, the ulceration had nearly disappeared and so she could save her pre[ious toes. From this case, even though we could not say that Diabetic foot is equal to Ban-Chang-Byung but could suppose that there was some relationship between one and the other.

• 사상체질의학회지
• /
• 제14권3호
• /
• pp.97-113
• /
• 2002

Objective To study the effectiveness of Yuldahansotang against Allergic Contact Dermatitis, the change of cutaneous shape, histochemistry, immunohistochemistry and distribution of apoptotic cells was researched. materials and methods 4-month-old rats were divided into three groups of 10. One is a contrastive group which has applied Acetone olive oil only. Another is ACD group which has intentionally activated Allergic Contact Dermatitis by DNCB. And the other is YST group which has given medication of Yangkyuksanhawtang extract. Each group of mice were observed after 24, 48 and 72 hours. results 1. With the result of Contact hypersensitivity assay, YST group shows appreciably less ear swelling than ACD group. 2. Comparing YST and ACD groups to each other regarding general change of skin, YST group shows less hyperplasia of epidermis, less migration of inflammatory cells and less damage of epidermis than ACD group. 3. Regarding the change of collagen fiber, ACD group has appeared to be low in number of collagen fiber while YST shows similarity with the contrastive group. 4. In dermis YST group has showed lower number of mastocyte than ACD group and is granulated type. 5. In dermis YST group has showed less MAC-1,IL-1, $IL-2R-{\alpha}$, ICAM-1 and VCAM-1 than ACD group. 6. The distribution of apoptotic cells has appeared littler in YST group than in ACD 7. Among signal molecule of apoptosis Bcl-2 has distributed more in YST group than ACD group and Bax and Fas has distributed less in YST group than ACD group.

• 사상체질의학회지
• /
• 제13권1호
• /
• pp.88-96
• /
• 2001

To evaluate the effect of Yuldahansotang(YHT) water extract on cultuted mouse spinal sensory neuron which was inhibited by xanthine oxidase(XO) and hypoxanthine(HX)-induced oxigen radicals, MIT assay, NR assay, Neurofilament enzymeimmuno assay and LDH activity assay were carried our after the cultured mouse spinal sensory neuron were preincubated with various concentrations of YHT water extract for 3 hours prior to exposure of XO/HX. The results obtained were as follows: 1. XO/HX, a oxigen radical, decreased the survival rate of the cultured mouse spinal sensory neuron cells on NR assay and MTT assay. 2. MTT50 value and NR50 value pf XO/HX were 20 mU/ml XO/0.2 mM HX and 40 mU/ml XO/0.2 mM HX. 3. YHT water extract have efficacy of increasing neurofilament. 4. YHT water extract have efficacy of increasing LDH activity. From above the results, It is concluded that YHT has marked efficacy as a treatment for the damages caused in the XO/HX-mediated oxidative process.

• 사상체질의학회지
• /
• 제12권1호
• /
• pp.201-215
• /
• 2000

1. 연구배경 및 목적 사상의학(四象醫學)에서 열다한소탕(熱多寒少湯)은 태음인(太陰人)의 뇌경색증 급성기에 매우 빈번하게 사용되는 처방 중 하나이다. 그래서 저자는 열다한소탕(熱多寒少湯) 투여에 따른 뇌경색증 환자의 면역계 특히 세포활성문질만계의 변화에 대해 연구하기로 하였다. 2. 방법 원광대학교 광주한방병원 사상의학과에 입원한 뇌경색증 한자 8인(남자 3명, 여자 5명)을 대상으로 하여, 내원 당시와 2-4주간의 열다한소탕 투여 후의 세포활설물질망계의 변화를 추적하였다. 또한 정상태음인의 말초혈액단핵구를 채취하여 리포다당체 처리시의 변화를 열다한소탕(熱多寒少湯) 전처리 유무에 따라 비교 분석하였다. 3. 결과 IL-2 혈장 농도는 정상 대조군보다 환자군에서 더 낮았다. $IFN-{\gamma}$의 경우 역시 정상 대조군보다 약간 낮았으나 통계학적 유의성은 없었다. IL-4, IL-6, IgE의 혈장중 수준은 정상 대조군에 비해 환자군에서 현저히 상승되어 있었다. 그러나, 2-4주간의 열다한소탕(熱多寒少湯) 투여후 $IFN-{\gamma}$, IL-2의 혈장 중 수준은 현저히 상승되었고, IL-4, IL-6, Ig E 수준은 현저히 억제되었다. 한편 정상 태음인의 말초혈액단핵구에 리포다당체 처리를 한 후 $IFN-{\gamma}$, IL-2, IL-6가 상승되었으나, 열다한소탕(熱多寒少湯) 전처리를 한 경우에서는 이들 물질의 생성이 유의하게 억제되었다.

• 사상체질의학회지
• /
• 제10권1호
• /
• pp.285-293
• /
• 1998

Yuldahansotang(YH) has been used in Sasang(四象) constitution medicine for many years as a therapeutic agent for cerebral disease. The effect of YH on the vascular system is not known. The purpose of this study was to determine the effect of YH on blood pressure, regional cerebral blood flow(rCBF) and pial arterial diameter of rats. 1. Blood pressure decreased by YH in rats. 2. rCBF was increased by YH in a dose-dependent manner. 3. Pretreatment with propranolol, methylene blue and indomethacin significantly inhibited YH induced increase in rCBF. 5. Blood pressure increased by Radix Puerariae(RP) and Radix Ligustici Tenuissimae(RLT) but Radix Scutellariae(RC) decreased blood pressure in rats. 6. rCBF was increased by RP and RLT in a dose-dependent manner but RC decreased low dosage, and RC increased high dosage. 7. Pial arterial diameter was increased by YH in a dose-dependent manner. 8. Pretreatment with propranolol significantly inhibited the increased in pial arterial diameter induced by YH. These results suggest that YH causes a diverse response of blood pressure, regional cerebral blood flow(rCBF) and pial arterial diameter. The increase in rCBF is also mediated by prostaglandins, cyclic GMP and adrenergic ${\beta}$ receptor and the increase in pial arteral diameter is mediated by adrenergic ${\beta}$ receptor.