Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
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Effects of Yuldahansotang water extract on Cultured Spinal Sensory Neurons Damaged by Xanthine Oxidase/Hypoxanthine

열다한소탕(熱多寒少湯) 전탕액(煎湯液)이 XO/HX에 의해 손상(損傷)된 배양척수감각신경세포(培養脊髓感覺神經細胞) 미치는 효과(效果)

  • Hong, Jeong-a (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Kim, Kyung-yo (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Yu, Do-gon (Dept. of Physiology, College of Oriental Medicine, Wonkwang Univ.) ;
  • Park, Hye-sun (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Kim, Hyung-soon (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.)
  • 홍정아 (원광대학교 대학원 한의학과) ;
  • 김경요 (원광대학교 대학원 한의학과) ;
  • 유도곤 (원광대학교 한의학과 생리학교실) ;
  • 박혜선 (원광대학교 대학원 한의학과) ;
  • 김형순 (원광대학교 대학원 한의학과)
  • Published : 2000.12.31
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Abstract

To evaluate the effect of Yuldahansotang(YHT) water extract on cultuted mouse spinal sensory neuron which was inhibited by xanthine oxidase(XO) and hypoxanthine(HX)-induced oxigen radicals, MIT assay, NR assay, Neurofilament enzymeimmuno assay and LDH activity assay were carried our after the cultured mouse spinal sensory neuron were preincubated with various concentrations of YHT water extract for 3 hours prior to exposure of XO/HX. The results obtained were as follows: 1. XO/HX, a oxigen radical, decreased the survival rate of the cultured mouse spinal sensory neuron cells on NR assay and MTT assay. 2. MTT50 value and NR50 value pf XO/HX were 20 mU/ml XO/0.2 mM HX and 40 mU/ml XO/0.2 mM HX. 3. YHT water extract have efficacy of increasing neurofilament. 4. YHT water extract have efficacy of increasing LDH activity. From above the results, It is concluded that YHT has marked efficacy as a treatment for the damages caused in the XO/HX-mediated oxidative process.

Keywords

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