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Immune Cell Activation and Co-X-irradiation Effect of Eleutherococcus senticosus Maxim Root (가시오갈피 뿌리의 면역세포 활성 및 방사선 병용효과)

  • Kwon, Hyoung-Cheol;Park, Jeong-Seob;Choi, Dong-Seong
    • Radiation Oncology Journal
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    • v.25 no.3
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    • pp.185-191
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    • 2007
  • Purpose: This study was performed to investigate the effects of immune cell activation and the antitumor effect for the combination of treatment with X-irradiation and E/eutherococcus senticosus Maxim Root (ESMR) on mouse tumor cells. Materials and Methods: ESMR (250g) was extracted with 80% methanol, concentrated under decompression and lyophilized. To determine whether ESMR is able to activate the immune cells or not, the proliferation of splenocytes in vitro and the number of B cells and T cells in splenic lymphocytes in ESMR-pretreated mice were evaluated. X-irradiation was given to the mouse fibrosarcoma tumor cells (FSa II) by 250 kv X-irradiation machine. The cytotoxicity of ESMR was evaluated from its ability to reduce the clonogenecity of FSa II cells. In X-irradiation alone group, each 2, 4, 6 and 8 Gy was given to FSa II cells. In X-irradiation with ESMR group, 0.2 mg/ml of ESMR was exposed to FSa II cells for 1 hour before X-irradiation. Results: The proliferation of cultured mouse splenocytes and thymocytes were enhanced by the addition of ESMR in vitro. The number of B cells and T cells in mouse splenic lymphocytes was significantly increased in ESMR pretreated mice in vivo. In FSa II cells that received a combination of 0.2 mg/ml of ESMR with X-irradiation exposure, the survival fraction with a dose of 2, 4 and 6 Gy was $0.39{\pm}0.005$, $0.22{\pm}0.005$ and $0.06{\pm}0.007$, respectively. For FSa II cells treated with X-irradiation alone, the survival fraction with a dose of 2, 4 and 6 Gy was $0.76{\pm}0.02$, $0.47{\pm}0.008$ and $0.37{\pm}0.01$. The difference in the survival fraction of the mouse FSa II cells treated with and without ESMR was statistically significant (p<0.05). Conclusion: Treatment with ESMR increased cell viability of mouse splenocytes in vitro and especially the subpopulation of B cells and T cells in splenocytes in ESMR-pretreated mice. However, treatment with ESMR did not increase the level of Th and Tc subpopulations in the thymocytes. Treatment with the combination of ESMR and X-irradiation was more cytotoxic to mouse tumor cells than treatment with X-irradiation alone; this finding was statistically significant.

Stability of Co/Ni Silicide in Metal Contact Dry Etch (Co/Ni 복합실리사이드의 메탈 콘택 건식식각 안정성 연구)

  • Song Ohsung;Beom Sungjin;Kim Dugjoong
    • Korean Journal of Materials Research
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    • v.14 no.8
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    • pp.573-578
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    • 2004
  • Newly developed silicide materials for ULSI should have the appropriate electrical property of low resistant as well as process compatibility in conventional CMOS process. We prepared $NiCoSi_x$ silicides from 15 nm-Co/15 nm-Ni/Si structure and performed contact dry etch process to confirm the dry etch stability and compatibility of $NiCoSi_x$ layers. We dry etched the photoresist/SiO/silicide/silicon patterns with $CF_4\;and\;CHF_3$ gases with varying powers from 100 to 200 W, and pressures from 45 to 65 mTorr, respectively. Polysilicon and silicon active layers without silicide were etched $0\sim316{\AA}$ during over etch time of 3min, while silicon layers with proposed $NiCoSi_x$ silicide were not etched and showed stable surfaces. Our result implies that new $NiCoSi_x$ silicides may replace the conventional silicides due to contact etch process compatibility.

X-Ray Fluorescence Analysis by Stearic Acid-Extraction Technique (스테아르산 추출법에 의한 X-선 형광분석)

  • Tae Sub O;Man Ho Lee;Young Kyu Park
    • Journal of the Korean Chemical Society
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    • v.28 no.1
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    • pp.41-46
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    • 1984
  • To preconcentrate trace elements, microgram amounts of 5 heavy metals (Cu, Co, Ni, Zn and Cd) were precipitated with 8-hydroxyquinoline (oxine) and metal oxinates were extracted with stearic acid. And then each of the molten stearic acid extract with stearic acid. And then each of the molten stearic acid extract was poured into a glass ring and cooled for specimen preparation. The obtained specimens were analyzed by X-ray fluorescene spectrometry. And then conditions of precipitation formation and extraction, reproducibility, sensitivity and detection limit were observed. The relative standard deviation of specimen preparation was 1.0~5.7% and the detection limit was 5~$50{\mu}g$/100ml. The proposed preconcentration procedure exhibited a considerable inhancement and simplicity in preparing specimens.

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Convergence study of oxidative stress from fraction of Xanthium strumarium L. (도꼬마리 추출물의 산화적 스트레스에 대한 융합연구)

  • Min, Young-Sil
    • Journal of the Korea Convergence Society
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    • v.8 no.12
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    • pp.215-220
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    • 2017
  • Xanthium strumarium L. is an annual plant belongs to the family Asteraceae which is is called a 'Cocklebur' that is used for medicinal purposes. Convergent phyto-activity of various extracts of Xanthium strumarium L. (Asteraceae) was examined. We estimated antioxidant activity from ground part and fruit extract of X. strumarium using 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) and ABTS assay. The extract of X. strumarium was separated each fraction that of ethanol, petroleum ether, and ethyl acetate. It showed potent radical scavenging effect against the DPPH radical and ABTS. The study revealed that X. strumarium could be used as a potential source of natural antioxidant.

Estimate of Ships Emission in Busan Port during 2009 Based on Activity (활동도를 이용한 2009년도 부산항 선박배출량 산정에 관한 연구)

  • Park, Doo-Yeol;Hwang, Cheol-Won;Jeong, Chang-Hun;Shon, Zang-Ho
    • Journal of Environmental Science International
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    • v.20 no.5
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    • pp.599-610
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    • 2011
  • Emission of air pollutants such as nitrogen oxides ($NO_x$), hydrocarbons (HC), $SO_2$, and particulate matter (PM) and $CO_2$ from ship during 2009 in Busan port was estimated based on activity-based method. The significant fraction (> 50%) of ship emission resulted from container and general cargo ships. Emission at port operation mode was the most dominant compared to at sea and maneuvering modes. Emission at North port was the largest source of air pollutants among ports. The magnitudes of air pollutants $NO_x$, $SO_2$, HC, $CO_2$, and PM in Busan port were $8.7{\times}10^3$, $8.23{\times}10^3$, $0.35{\times}10^3$, $4.86{\times}10^6$, and $0.67{\times}10^3$ ton/yr, respectively. The ratio of $NO_x$ to VOC is about 25. Our ship emission estimate is 2 times higher than that in CAPSS emission inventory.

An Investigation of Heat Transfer Characteristics of Swirling Flow in a 180$^{\circ}$ Circular Section Bend with Uniform Heat Flux

  • Chang, Tae-Hyun
    • Journal of Mechanical Science and Technology
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    • v.17 no.10
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    • pp.1520-1532
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    • 2003
  • An experiment was performed to obtain the local heat transfer coefficient and Nusselt number in a circular duct with a 180$^{\circ}$ bend for Re=6 x 10$^4$, 8 x 10$^4$ and 1 x 10$\^$5/ under swirling flow and non-swirling flow conditions. The test tube with a circular section was made from stainless steel having a curvature ration of 9.4. Current heat flux of 5.11 kW/㎡ was applied to the test tube by electrical power and the swirling motion of air was produced by a tangential inlet to the pipe axis at 180$^{\circ}$. Measurements of local wall temperatures and the bulk mean temperatures of air were made at four circumferential positions at 16 stations. The wall temperatures showed a reduced distribution curve at the bend for the non-swirling flow, but this effect did not appear for the swirling flow. The Nusselt number distributions for the swirling flow, which was calculated from the measured wall and the bulk temperatures, were higher than that of the non-swirling flow. The average Nusselt number of the swirling flow increased by about 90-100%, compared to that of the non-swirling flow. The Nu/Nu$\_$DB/ values at the 90$^{\circ}$ station for non-swirling flow and swirling flow were approximately 2.5 and 4.8 at Re=6x10$^4$ respectively. The values agree well with Said's results for non-swirling flow.

The Geometric Modeling for 3D Information of X-ray Inspection (3차원 정보 제공을 위한 X-선 검색장치의 기하학적 모델링)

  • Lee, Heung-Ho;Lee, Seung-Min
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.62 no.8
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    • pp.1151-1156
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    • 2013
  • In this study, to clearly establish the concept of a geometric modeling I apply for the concept of Pushbroom, limited to two-dimensional radiation Locator to provide a three-dimensional information purposes. Respect to the radiation scanner Pushbroom modeling techniques, geometric modeling method was presented introduced to extract three-dimensional information as long as the rotational component of the Gamma-Ray Linear Pushbroom Stereo System, introduced the two-dimensional and three-dimensional spatial information in the matching relation that can be induced. In addition, the pseudo-inverse matrix by using the conventional least-squares method, GCP(Ground Control Point) to demonstrate compliance by calculating the key parameters. Projection transformation matrix is calculated for obtaining three-dimensional information from two-dimensional information can be used as the primary relationship, and through the application of a radiation image matching technology will make it possible to extract three-dimensional information from two-dimensional X-ray imaging.

Separation of X- and Y-Bearing Spermatozoa III. Separation of bull spermtozoa by Sephadex Gel Filtration (X-정자와 Y-정자의 분이에 관한 연구 III. Sephadex Gel 여과에 의한 우정자의 분이)

  • 이주영;엄기붕;고대환;김종배;정길생
    • Korean Journal of Animal Reproduction
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    • v.12 no.1
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    • pp.24-30
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    • 1988
  • These experiments were carried out to develop new techniques for In Vitro separatin of X-and Y-bearing spermatozoa. The bull semen was applied to the various Gel-Columns filled with swellen Sephadex G-50 Fine and then elutriated wtih Locke solution (elutriation rate; 1ml/3-4min., 1ml/1-2min.). Elutriated solution was fractionated into 1ml by automatic Fraction Collector and spermatozoa included in each fraction were subjected to the estimation of viability and recovery rate, and to B-body test. The results obtained in these experiments were summarized as follows: 1. When the column size and the elutriation rate were adjusted to 15$\times$1.6cm and 1ml/3-4min., respectively, the highest sperm concentration was obtained from the 8th to the 12th fraction. 2. As a trend, the viability of spermatozoa was improved by chromatography, and the degree of improvement ranged 5 to 10 percentage. 3. The average recovery rate of spermatozoa applied to column was 73.2 percentage and ranged 52.6 to 81.3 percentage. 4. The lowest rate of B-body bearing spermatozoa following chromatography was obtained when the column size and the elutriation rate were adjusted to 15$\times$0.8cm and 1ml/1-2min., respectively.

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Design of Miniaturized Multi-layer BPFs Using LTCC for Wireless LAN Applications (LTCC를 이용한 WLAN용 초소형 적층 대역통과 필터 설계)

  • Park, Hun;Kim, Kuen-Hwan;Yoon, Kyung-Sik;Lee, Young-Chul;Park, Chul-Soon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.28 no.7A
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    • pp.563-568
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    • 2003
  • In this paper, a miniaturized parallel coupled bandpass filter using multi-layered LTCC(Low Temperature Co-fired Ceramics) substrate for SOP(System-On-Package) is proposed for applications to wireless communication systems. The fabricated BPF is composed of five 106${\mu}{\textrm}{m}$ thick LTCC layers and its size is 5.24mm x 4.3mm x 0.53 mm. The measured characteristics of the BPF show the center frequency of 5.8GHz, bandwidth of 200MHz, insertion loss of 2.326dB and return loss of 13.679dB. In addition, the attenuation is 28.052dB at 4.7GHz.

Origin of the Initial Permeabiliy of Ni0.8-xZn0.2CoxFe2O4+δ Ferrite (Ni0.8-xZn0.2CoxFe2O4+δ조성 페라이트의 투자율 변화 기구)

  • 안용운;김종령;오영우
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.17 no.1
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    • pp.46-51
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    • 2004
  • The cause for the variation of the initial permeability according to the Co substitution of Ni-Zn ferrite used in the LC resonance filter for the power line communication is studied. The initial permeability decreases as the quantity of Co diminishes, and the saturation magnetization increases as the quantity increases. Because the sintering density and the microstructure of ferrite show little change, the variation of the initial permeability can't be explained by the density, microstructure nor the saturation magnetization factor. The magnetocrystalline anisotropy increases, similar with the saturation magnetization, as the quantity of Co increases. The increase of magnetocrystalline anisotropy value makes the domain wall energy grow, which leads to the decrease of the initial permeability, because there's linear law between the magnetocrystalline anisotropy and the domain wall energy. The resonance frequency to Co substitution moved to high frequency band, due to the close relationship with domain wall energy, Initial permeability decreaed a little with an increase of Co contents, but resonace frequency moved to high frequency band. as a result of that, when Co was added 0.05 mol, initial permeability and resonace frequency was 75 and 25 MHz respectively.