• Korean Journal of Pharmacognosy
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• v.47 no.3
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• pp.264-272
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• 2016

This study was carried out to evaluate the preventive effect of the Corni Fructus (SSU) 50 % EtOH extract (SSU-E50) against bisphenol A (BPA) toxicity in Leydig cells and improving testosterone deficiency syndrome in orchidectomized Sprague-Dawly (SD) rats. Antioxidant properties were measured by radical scavenging activity of SSU-E50 in ABTS assay and DPPH assay. Also, real-time polymerase chain reaction(real-time PCR) was performed to quantify the mRNA expression levels of antioxidant enzyme. SD rats were divided into eight group: normal, sham operation (Sham), orchidectomized (ORX), ORX treated with testosterone 1 mg/kg (Tes. 1), ORX treated with SSU water extract 100 mg/kg (SSU-A 100) and 300 mg/kg (SSU-A 300), ORX treated with SSU 50 % EtOH extract 100 mg/kg (SSU-E 100) and 300 mg/kg (SSU-E 300). On a comparative basis, the SSU showed better activity quenching ABTS with an IC50 value of 0.29 mg/ml and DPPH with an IC50 value of 0.33 mg/ml. Cell viability was evaluated by MTS assay as described not cytotoxic at the highest concentration of $500{\mu}g/ml$. Cytotoxicity of BPA showed in $200{\mu}M$, but definitely survived by treatment with SSU in Leydig cells. In addition, SSU increased the mRNA expression levels of antioxidant enzyme in BPA induced Leydig cells. Superoxide dismutase (SOD) level was slightly increased and malondialdehyde (MDA) level was decreased with SSU-A 100 in in-vivo. These results suggest that Corni Fructus extracts have the greatest property as a natural anti-oxidative and improves testosterone deficiency syndrome source.

• Proceedings of the Ginseng society Conference
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• 1988.08a
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• pp.70-76
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• 1988

Since ancient times, records pertaining to chi-reinforecenent of ginseng have been documented in books of Chinese nedicine. We, therefore, assessed the therapeutic effects of ginseng on patients with chi-deficiency syndrome. Eigheen cases of chi-deficiency, screened at the outpatient service in the internal department of Chinse medicine at the China Medical College Hospital. were prescribed two grams of Korean red ginseng powder three times daily for a two week period. For evaluation, a questionnaire and diagnosis by sphygmography were conducted prior to and after administration of the prescription. For control purposes, fourteen volunteers fasted for more than five days (seven had access only to ginseng. and the other seven were given only mineral water) and were subsequently evaluated by the same criteria. Three results were obtained for the ginseng treated group: (1) improvements in symptoms of chi-deficiency such as fatigue and dizziness; (2) changes in pulse waves with time domains showing alternate P-waves, strengthened dicrotic waves, and some minute waves in the end-diastolic period; (3) frequency domains with enhanced amplitudes around 12Hz. These three findings, which are consistent with each other, suggest the reinforcement of the effect of ginseng on 'heart-spleen chi' in Chinese medicine.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.25 no.1
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• pp.79-86
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• 2022

Purpose: To determine the effect of zinc deficiency on fecal protein, electrolyte, and short-chain fatty acid levels in both heat-stable (ST) and heat-labile (LT) enterotoxigenic Escherichia coli (ETEC)-induced diarrhea in rats. Methods: Albino rats, weighing 100 to 150 g, were divided into 2 groups, with 15 animals each: non-zinc and zinc-deficient. These two groups were sub-divided into three sub-groups with five rats each: control (saline); LT-ETEC; and ST-ETEC. Sodium phytate (30 mmol/L) was added to the animals' water to induce zinc deficiency, while diarrhea was induced using 5×10⁹ ETEC cells/mL. Fecal protein levels were estimated using the Bradford method, while sodium and potassium levels were determined using atomic absorption spectrophotometry. Short-chain fatty acids were measured using gas chromatography-mass spectrometry. Results: Among the non-zinc and zinc-deficient groups, there were significant increases (p=0.04), (p=0.03) in fecal protein concentrations (mg/mL) in the LT-ETEC- (4.50±0.33), (6.50±0.26) and ST-ETEC- (3.85±0.19), (5.98±0.32) induced groups compared to the control groups (2.60±0.52), (3.50±0.11) respectively. Fecal sodium and potassium levels (mg/L) were significantly (p=0.029) increased in non-zinc-deficient rats induced with LT-ETEC (9.35±0.95, 1.05±0.48), and ST-ETEC (9.96±1.02, 1.21±0.45) compared with the control group (8.07±0.44, 0.47±0.17) but the increase were not statistically significant (p=0.059) in the zinc deficient rat groups. Fecal acetate and propionate levels (mg/g) significantly (p=0.032) increased when induced with LT-ETEC and ST-ETEC in non-zinc and zinc-deficient groups compared with the control groups. Conclusion: Zinc deficiency among rats with ETEC-induced diarrhea elevated fecal protein loss but may not have an effect on fecal sodium, potassium and short-chain fatty acid levels.

• KCID journal
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• v.17 no.2
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• pp.57-70
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• 2010

This study was performed to analyze the affect of water supply capacity followed by allocating flood control volume in heightening reservoir, of which Baekgog reservoir was selected as a case study in here. Baekgog reservoir is located in Jincheon county, Chungbuk province, of which full water level will be heightened from EL. 100. 1m to EL. 102.1m, and total storage from 21.75M $m^3$ to 26.67M $m^3$. Flood inflow with 200year frequency was estimated to 997 $m^3$/s in peak flow and 22.54M $m^3$ in total volume. Reservoir flood routing was conducted to determine flood limited water levels, which was determined to have scenarios such as EL 97-98-99m in periods of 6.21.-7.20., 7.21.-8.20., and 8.21.-9.20., respectively, EL 97-97-97m, EL 98-98-98m in present reservoir, and EL 99-100-101m, EL 99-99-99m, and EL 100-100-100m in heightened reservoir. Reservoir inflow was simulated by DAWAST model. Annual paddy irrigation requirement was estimated to 33.19M $m^3$ to 2,975ha. Instream flow was allocated to 0.14mm/d from October to April. Operation rule curve was drawn using inflow, irrigation and instream flow requirements data. In case of withdrawal limit reservoir operation using operation rule curve, reduction rates of annual irrigation supply before and after flood control by reservoir were 2.0~4.3% in present size and 1.5~3.6% in heightened size. Reliability on water supply was decreased from 77.3% to 63.6~68.2% in present size and from 81.6% to 72.7~79.5% in heightened size. And reduction rates of water storage at the end of year before and after flood control by reservoir were 7.3~16.5% in present size and 7.7~16.9% in heightened size. But water supplies were done without any water deficiency through withdrawal limit reservoir operation in spite of low flood regulating water level.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.4
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• pp.52-60
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• 2004

In order to analysis of the water pollution of the Anyang stream(one of the biggest branch streams of the Han River) and its main tributaries, the geological and topographical and rainfall features in its basin were investigated. To do this, the water samples were collected 23 points of the upper, mid and down of Anyang stream and its tributaries and were analyzed based on the chemical methods, Korean Biotic Index(KBI) and Saprobien systems. The Anyang stream basin has a characteristic of topographical torrential heavy rainfall like a typical rainfall feature in Korea. The concentration and the outflow rate of rainfall is very different in seasonal, and water pollution in dry season is especially severe. After 1997, although the water quality status of stream has been improved gradually, the concentration of T-N and SS at the upstream is increased due to the deficiency of facility used for collecting wastewater released from industrial factories, livestock farms and residential areas. The mainstream of the Anyang stream is classified into the 5th grade water as polysaprobic water area according to Saprobien system and the biotic index is over 2.5 in overall. Most of tributaries have 1~3 grade water limit with ${\alpha}$- and ${\beta}$-mesosaprobic except the upper and mid streams of Samsung tributary, in which pollution status is the lowest part. Though Sambong tributary is ${\alpha}$-mesosaproboic, biotic index is low because of the appearance of abundant benthos animals in farming and fishing villages.

• Journal of Korea Water Resources Association
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• v.44 no.4
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• pp.315-325
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• 2011

There are not many practical measures to solve a water conflict, when a hydromorphologically asymmetric situation in international rivers exists whereby downstream users may not affect upstream users but upstream users do cause downstream impacts. In taking advantage of this merit, North Korea has built Imnam Dam in upstream of North Han River and uses water for trans-basin hydropower generation. As an impact of this dam South Korean' area as a downstream user has been suffered from water deficit and dry river. It is very critical for South Korea to solve a key problem such as water allocation for water supply and river maintenance. Therefore, this study is aim to suggest alternatives for equitable water allocation in consideration of special circumstances between the South and the North. For this, reviewing the allocation methods of water rights is carried using lessons obtained from international river cases. The results show that the minimum desired streamflow is calculated at 7.3 $m^3/sec$; water budget analysis by the equitable distribution of streamflows at the border line of the North Han River, the difference in water supply deficiency is at 3.7 $m^3/sec$ before and after Imnam Dam; in the determined distribution method, the difference in water deficiency is at 11.38 $m^3/sec$. These results show that South Korea should be secured 11.38 $m^3/sec$ from North Korean's Imnam Dam in respective of water use right and sound river maintenance.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.19 no.2
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• pp.109-124
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• 2014

In order to understand the carbon cycle in the Amundsen Sea of the Southern Ocean, the export fluxes of particulate organic carbon from the euphotic zone to deep water estimated using ${\psi}$/${\psi}$ disequilibrium method. Seawaters in 14 water columns were collected during February and March 2012, and analyzed for total and dissolved ${\psi}$, and particulate organic carbon. Total ${\psi}$ activities in the water column showed deficiency and excess relative to those of ${\psi}$ depending on the water depth. Deficiency of total ${\psi}$ in the euphotic zone showed mirror images both with chlorophyll-a and fluorescence, and was consistent with the loss of nitrate, which indicated the effect of biological activity. In addition, deficiency of total ${\psi}$ from deep water was associated with the increase of total dissolvable Fe/Mn concentration. Excess total ${\psi}$ activity presented below the euphotic zone might be related to particulate ${\psi}$ concentrated in this water depth. Mean export flux of ${\psi}$ estimated using the steady state model was $867{\pm}246dpmm^{-2}day^{-1}$. Mean export flux of particulate organic carbon, which were estimated by the product of total ${\psi}$ flux and ratio of POC/${\psi}$ ($7.08{\pm}4.27{\mu}molCdpm^{-1}$) in the sinking particles, was $5.9{\pm}3.9mmolCm^{-2}day^{-1}$. These fluxes were similar levels to those in the Weddell Sea during February and March 2008. Export ratios (ThE) relative to the primary production in the euphotic zone were in the range of 3-54% (av. 28%).

• Journal of Korean Society on Water Environment
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• v.30 no.6
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• pp.665-672
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• 2014

Elemental cellular stoichiometry of natural phytoplankton communities was examined in six large dam reservoirs in the Han River system. Carbon (C) and nitrogen (N) contents of the phytoplankton-dominated seston from Lake Soyang was higher than that from other lakes. Phosphorus (P) content showed slight variations among six lakes in the range of $0.04{\sim}0.18{\mu}mol$ P mg $DW^{-1}$. The phosphorus concentrations of lake water showed obvious positive relationship with P and C contents of the phytoplankton-dominated seston. In all six lakes, N:P ratios and C:P ratios in the phytoplankton-dominated seston were more than 23 and 133 respectively, indicating the phytoplankton communities in six lakes have been exposed in phosphorus limited condition. The relative abundance of diatoms showed significant negative correlation with C, N, P contents of the phytoplankton-dominated seston and that of cyanobacteria showed significant positive correlation. Elemental stoichiometry of diatoms-dominated seston showed distinctive less content than that of cyanobacteria-dominated seston. The cellular N:P ratios of diatoms- and cyanobacteria-dominated seston indicate that both main classes of phytoplankton in six lakes are in phosphorus deficiency. Elemental stoichiometry of the phytoplankton-dominated seston in this study could be used for the further ecological stoichiometric studies in six lakes.

• Journal of Nutrition and Health
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• v.21 no.2
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• pp.113-121
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• 1988

The purpose of this study was to investigate the effects of dietary zinc on immune response in mice. Weanling male mice was placed individually in stainless steel cages and fed a zinc dificient diet and control diet. All mice were given deionized water ad libitum. The introduction of extraneous zinc was minimized in all cage by washing feed jars and water bottles sequentially with 4mM EDTA and conc-nitiric acid followed by deionized water. After 4 and 5 weeks of the diets, mice were immunized with lx 106 Naegleria fowleri intraperitoneally. Mice were weighed once a week. The results from this study are summarized as followed ; 1) Mice fed the zinc dificient diet showed growth retardation. After 3 weeks of diets, mean body weight of zinc deficient mice was 21.4g and that of control was 25.0g. This difference is singnificant statistically (p<0.01). The more time passed, the more remarkable difference was found. 2) The weigth of organs were measured on liver, kidney, spleen, thymus, heart, lung, brain. Difference in weight were observed only in liver and spleen. 3) Proliferative response of spleen cells of zinc deficient mice to con A was lower than that of control mice after one week on immunization(p<0.005). 4) Stimulation index was lower in zinc deficient mice to phytohemagglutinin after two weeks on immunization (p<0.05). 5) Blastogenesis of speen cells of zinc deficient mice to Naegleria fowleric lysate was lower after 10 days on immunization (p<0.05). 6) Immunoglobulin G antribody titers of zinc deficient mice sera by ELISA was lowered to control mice after 5 weeks on immunization (p<0.005).

• Herbal Formula Science
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• v.19 no.2
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• pp.61-71
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• 2011

Objectives : Kyungok-go(KOG), the first herbal formulation of donguibogam, has been used for treating of many symptoms of yin deficiency. In this study, we examined the effect of KOG on bone resorption. Methods : We determined the effects of water extract of KOG in RANKL(Receptor Activator for Nuclear Factor ${\kappa}B$ Ligand)-induced osteoclast differentiation culture system and osteoblast proliferation. In addition, we determined the effects of water extract of ABR on LPS-induced bone-loss with mice. Results : Water extract of KOG showed proliferation effect on osteoblast without cytotoxicity and no effect on RANKL-treated osteoclast differentiation. KOG rescued bone erosion by LPS induction in vivo study. Conclusions : These results demonstrated that KOG can be a useful remedy for treating of bone-loss disease such as osteoporosis.