• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.11
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• pp.1709-1715
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• 2014

The objective of this study was to examine the antioxidant activities of extracts from Mori ramulus using different extraction methods (HE, hot water extraction; EE, 50% ethanol extraction; UE, ultrasonic extraction; PE, pressured extraction). The extraction yield of PE (3.07%) was higher than that of UE (1.43%), EE (1.18%), and HE (1.07%). The total phenolic and flavonoid contents of EE were 334.66 mg/g, and 35.64 mg/g, respectively. The oxygen radical absorbance capacity (ORAC) of EE ($3,483.37{\mu}M/g$ FW) was higher than those of HE ($2,687.52{\mu}M/g$ FW), UE ($2,300.45{\mu}M/g$ FW), and PE ($2,117.62{\mu}M/g$ FW). The DPPH and ABTS radical scavenging activities of EE at $1,000{\mu}g/mL$ were 65.84%, and 97.52%, respectively. The superoxide radical scavenging activity of EE was 67.77~98.74% ($100{\sim}500{\mu}g/mL$) higher than those of other extracts. The ferric reducing antioxidant power and reducing power of EE were $189.00{\sim}974.80{\mu}M$, and 0.12~0.82, respectively. The tyrosinase inhibitory activity of EE (23.25~67.20%) improved with an increase treatment concentration. The antioxidant and tyrosinase inhibitory activities of EE were significantly higher than those of other extracts. In conclusion, we provided experimental evidence that extracts from Mori ramulus have potential as functional materials.

• Journal of Mushroom
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• v.8 no.3
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• pp.131-136
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• 2010

This study was performed to determine the optimal concentration of carbon dioxide, which effects mushroom growth and yield. It was shown that the periods for fruiting initiation, growth and harvest of Flammulina velutipes were increased when the $CO_2$ concentration was raised. In general, those characteristics were less affected in brown strains than in white ones. Especially brown strain ASI4103 was susceptible to changes in $CO_2$ concentration. Yields per bottle and individual mushroom weight also decreased in most strains when $CO_2$ levels increased. We were unable to designate any tendency in the number of fruiting bodies due to the large variation within each respective strain. Finally, water contents in the fruiting bodies were found to decline under high $CO_2$ concentrations.

• Journal of Mushroom
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• v.8 no.3
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• pp.116-121
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• 2010

To elucidate the effect of humidity to the characteristics related to mushroom cultivation, five white strains and four brown strains of Flammulina velutipes were cultivated and investigated on their characters. The periods for fruiting initiation, growth and harvest were a little decreased when humidity increased after peak at 75%. Brown strains showed fast fruiting than white ones. ASI 4103 was the fastest and ASI 4166 and ASI 4153 were the latest. The productivity of ASI 4166 and ASI 4149 were the best in all conditions. In general, white strains showed high yields at 75% and brown ones did at 95%. Individual mushroom weight decreased and water content of mushroom increased in most strains when humidity increased.

• The Journal of Engineering Geology
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• v.11 no.1
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• pp.1-11
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• 2001

The geological characteristics of Korea are that we can encounter the rock layer only after 10m of excavation, methods to presume the rock pressure distribution of the rock layer is urgently needed. When using the existing empiric science of Terzaghi-Peck, Tschebotarioff to measure the rock pressure of the rock layer, underestimate the real strength because of the cohesion is ignored. Therefore calculating the horizontal sliding force of wedge block, which includes the dips and shear strength of discontinuities and surcharge load etc., think to be to getting a closer rock stress of the real rock pressure acting upon the earth structure in rock mass. This research use Coulomb soil pressure theory assuming that the backfill soil will yield wedge failure when it has cohesion, applying Prakash-Saran(l963), and then it uses equilibrium of force and shear strength $\tau$=c+$\sigma$tan $\Phi$ of the cliscontinuities. Analyzing shear strength and dips of cliscontinuities using calculated theory according to the status of discontinuities aperture, we were able to find out that because the cohesion and friction angle of the rock layer itself is large enough, how the dip directions and dips facing the excavation face is the only factor deciding whether or not the rock stress is applied. The evaluated theory of this research should be strictly estimated, so that the many parameters such as c, $\Phi$value, types and structures of rock class, excessive lateral pressure, dynamic load, earthquake, needed later when calculating shear strength of discontinuities and especially the ground water effect acting on rock layer should be coumpted with many measuring data achieve at the insite to study the application.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.4
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• pp.378-383
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• 1997

Bird damages are often occurred at the seeding and seedling stages of crops. What the bird damage is preventing from the seeding and seedling of rice in the paddy would be very important to get high yield. Methiocarb was formulated as a bird repellent of crop seeding and seedling. Its effects on the seedling growth, related to the chemical injuries and the bird damages, were examined at the seeding and seedling growth stages of Anjungbyeo(Oryza sativa L.) with different types and amounts of methiocarb treatment in both pot and field experiments. In the pot experiment, bird damages were not occurred at all treatments of methiocarb, while bird damages were occurred at the control; 30, 85, and 100％ of damages at 5, 10, and 15 days after seeding, respectively. The ratio of seedling stand was not significantly different among all of treatments, methiocarb, methiocarb＋thiram, and control. However, the plant height and the number of seedling leaves at 15 days after seeding was shortened and lowered statistically more at treatment of methiocarb 15g than those of methiocarb 10g and control. The plant height at 35 days after seeding, showing the recovery degrees of chemical injuries, was significantly lowered in treatment of methiocarb＋thiram 15g, whereas the plant height of methiocarb FS 15g was not significantly differed from that of the control. In the field study, the bird damages were significantly reduced in treatment of methiocarb compared to that of the control. The plant height and the number of leaves at 25 days after seeding were not differed statistically among all of treatments. The chemical injuries were occurred in all treatments of methiocarb at 10 days after seeding, while they were not shown at 20 days after seeding.

• Journal of Pharmacopuncture
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• v.19 no.4
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• pp.350-358
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• 2016

Objectives: This study was carried out to analyze the single dose toxicity of ShinEumHur (SEH) pharmacopuncture injected into the muscles of Sprague-Dawley rats. Methods: The SEH pharmacopuncture was made in a clean room at the Korean Pharmacopuncture Institute (K-GMP). After the mixing process with sterile distilled water had been completed, the pH was controlled to between 7.0 and 7.5. All experiments were conducted at Biotoxtech, an institution authorized to perform non-clinical studies under the Good Laboratory Practice (GLP) regulations. Sprague-Dawley rats were chosen for the pilot study. Doses of SEH pharmacopuncture, 0.25, 0.5 and 1.0 mL, were administered to the experimental groups, and a dose of normal saline solution, 1.0 mL, was administered to the control group. We examined the survival rate, weights, clinical signs, mean hematology parameters, mean clinical chemistry, necropsy and histopathological findings. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results: No deaths or abnormalities occurred in any of the four groups. No significant changes in weight, hematological parameters or clinical chemistry between the control group and the experimental groups were observed. To check for abnormalities in organs and tissues, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs or tissues. Conclusion: The above findings suggest that treatment with SEH pharmacopuncture is relatively safe. Further studies on this subject are needed to yield more concrete evidence.

• Polymer(Korea)
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• v.31 no.4
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• pp.329-334
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• 2007

Osmotic pellet system, which is one of the oral drug delivery systems, has been developed to improve manufacturing process, reduce product cost and other problems of osmotic tablet systems. Osmotic pellet is consisted of water swellable seed layer, drug layer, and membrane layer. Among them, the membrane layer plays an important role in a control of the drug release. In this work, we examined the effect of ratio for Eudragit RL and RS on the drug release behavior. Osmotic pellet with nifedipine as a model drug was easily obtained in a good yield by fluidized bed coater. Osmotic pellet showed round morphology with a range of size $1300{\sim}1500\;{\mu}m$. In the experiment of nifedipine release, the release amount increased with the increase of the ratio of Eudragit. This is due to the fact that Eudragit RL contains more hydrophilic quaternary ammonium group than Eudragit RS. Additionally, the release amount was retarded with increasing the membrane thickness. There are no differences in the release amount measured at the different pH 1.2, 6.5, 6.8, and 7.2. In conclusion, it was found that the drug release from osmotic pellets depended on the composition ratio and coating thickness of membrane layer.

• Journal of Life Science
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• v.17 no.9 s.89
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• pp.1237-1243
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• 2007

This study was investigated on the antioxidant capacity of 80% ethanol extract of lotus root in vitro. The ex-traction yields of 80 % ethanol extract was 9.14%. Lotus root ethanol extract was fractionated by the following: n-hexane, chloroform, ethylacetate and n-butanol. n-Butanol fraction showed the highest ex-traction yield of all fractions. Antioxidative activities of different fractions were examined by l.l-diphenyl-2-picrylhydrazyl(DPPH) radical generation, the Rancimat test, the nitrite scavenging ac-tivity and the thiobarbituric acid(TBA) method, and compared with the properties of the commercial antioxidant butylated hydroxytoluene(BHT). The antioxidative capacity of the ethylacetate fraction was the highest among fractions and its fraction showed higher contents of total polyphenol. Furthermore, the antioxidative capacity of the ethylacetate fraction was similar to that of BHT. In conclusion, these results suggest that lotus root may be a good candidate as a natural antioxidant source.

• Journal of Life Science
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• v.21 no.5
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• pp.746-752
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• 2011

The contents of bioactive materials (e.g. polyphenolics compounds, flavonoids, minerals, and fatty acids) and antioxidative activities (DPPH (${\alpha}$,${\alpha}$'-diphenyl-${\beta}$-picrylhydrazyl) free radical scavenging activity, peroxidation of linoleic acid and rat hepatocyte microsome, and Fe/Cu reducing power) were tested by in vitro experimental models using water, ethanol and methanol extracts of leaves (TOL) and fruits (TOF) from Thuja orientalis. Methanol extract from TOL showed the highest extraction yield (12.90%) as well as contents of polyphenolic compounds (16.02%) and flavonoids (0.25%). Major minerals were Ca, K, and Mg. Major fatty acids were palmitic and lauric acids in TOL and palmitic and decanoic acids in TOF. In oxidation of in vitro models using DPPH free radical scavenging activity, Fe/Cu reducing power, $Fe^{2+}$/ascorbate-induced linolenic acid peroxidation by ferric thiocyanate and thiobarbituric acid (TBA) methods, and autooxidation of rat hepatic microsomes membrane, anti-oxidative activities were stronger in all extracts of TOL than in those of TOF in a dose-dependent manner. From these results, methanol extract of TOL was shown to have the most potent anti-oxidative properties and the highes content of antioxidative compounds such as polyphenolic compounds and flavonoids.

• The Korean Journal of Mycology
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• v.27 no.6 s.93
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• pp.424-429
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• 1999

This study was conducted to investigate the characteristics of polysaccharides isolated from the fruit body and cultured mycelia of Phellinus linteus IY001. All fractions were extracted by hot water, followed by ethanol precipitation (F-THE and M-HE) or ultrafiltration (M-HU) (F-TH, F-THE; fruit body, M-HE, M-HU; cultured mycelia). Among these fractions, F-TH fraction was obtained at the highest yields of 6.83% and yield of F-THE was at the level 2.79%. The carbohydrates of these fractions was found to be a heteroglucan composed of glucose, galactose, mannose, fructose, ribose and xylose by analysis of gas chromatography. The total carbohydrate contents of M-HE and M-HU fractions were 99.2%, and 86.0% respectively. The glucose content of M-HE, M-HU and F-THE ranged from 54 to 84.8% of the total monosaccharide. Amino acid pattern showed that all fractions contained a large amount of aspartic acid, glycine, glutamic acid, alanine. Serine and threonine were found to be involved in the linkage, O-linked type. These fractions, except F. TH, contained polysaccharides with the molecular weights of 12 kD and showed the characteristics of IR absorption for ${\beta}-glucosides$ at $890\;cm^{-1}$.