• Title/Summary/Keyword: Washing time

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A Study on the Separation of X- and Y- Spermatozoa in Farm Animals (가축에 있어서 X-정자와 Y-정자의 분류에 관한 연구)

  • 고대환;박흠대;정길생
    • Korean Journal of Animal Reproduction
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    • v.3 no.1
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    • pp.41-47
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    • 1979
  • This experiment was carried out to clarify the methods of the F-body test in human and the B-body test in buil and hog. The effect of pH and albumin concentration on the migration of X- and Y- sperm was also investigated. The results obtained were summarized as follows: 1. In the human semen, the frequency of sperm in which an F-body is visible was different by the fluorochrome. Namely, in case of quinacrine mustard, the F-body frequency was 48.8∼43.4 percent (average 49.6%), and in case of quinacrine dihydrochloride, that was 40.7∼50.8 percent (average 42.0%). 2. The frequency of a, pp.rance of B-body was 43.4${\pm}$1.3 percent in bull semen, and 45.5${\pm}$0.7 percent in hog semen. 3. A, pp.arance of B-body in bovine semen was increased due to duration of time after washing till 12 hours. 4. Separation of X- and Y- spermatozoa using diluents with different hydrogen ion concentration was impossible. 5. A, pp.arance of B-body separated in medium with 6, 10 and 20% ovalbumin was 51.1${\pm}$2.4, 50.6${\pm}$2.5 and 58.2${\pm}$3.0 percent, respectively, and those values were significiantly higher (p<0.01) than corresponding control values.

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Reuse of Weaving Wastewater with BAF Process (BAF 공정을 이용한 제직폐수의 재이용)

  • Jung, Yong-Jun;Bae, Jong-Hong;Kwon, Koo-Ho;Min, Kyung-Sok
    • Journal of Korean Society on Water Environment
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    • v.20 no.6
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    • pp.664-669
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    • 2004
  • An upflow BAF(Biological Aerated Filter) equipped with an expanded clay media was applied to reuse weaving wastewater of water jet loom. The performance of lab-scale biofilter was investigated by the adjustment of EBCTs(Empty Bed Contact Time) and the packing ratio of media, which were changed 1.1 to 3.7hr and 38 to 63%, respectively. In most conditions except 1.1hr of EBCT, BOD, CODcr, SS and Turbidity of the effluent were 1~4mg/L, 7~16mg/L, 1~5mg/L and 5~14NTU, where their removal efficiencies were 76~95%, 82~93%, 63~94% and 59~81%, respectively. From the observation of SEM(Scanning Electron Microscope) photographs of porous clay media, it was revealed that this media provided good performance of retaining microbes effectively. In addition, $0.44~0.49kgVSS/kgBOD_{rem}$. of low sludge reduction was expected. The most efficient back washing cycle and procedure were once per 4 to 9 days and air including collapse-pulsing method, respectively. Therefore, this system can be of use as an weaving wastewater treatment for reuse.

Development of ELISA Method for the Determination of Compound K (Compound K 측정을 위한 ELISA법 개발)

  • Ryu, Mina;Li, Hai Guang;Sung, Jong Hwan;Sung, Chung Ki
    • Korean Journal of Pharmacognosy
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    • v.46 no.4
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    • pp.279-282
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    • 2015
  • In order to quantify compound K(CK), anticancer component of Panax ginseng C. A. Meyer, high titer rabbit polyclonal antibodies (pAbs) were raised against a conjugate of CK and bovine serum albumin coupled by a periodate oxidation method. Coating antigen (CK-OVA) was also prepared by the same method with OVA. As a result of optimization of antiserum dilution (2,000 fold), coating antigen ($25{\mu}g/ml$) and other condition (incubation time, temperature and washing method), ELISA method for the determination of CK was established. The measuring range extended from 0.5 ng/ml to 25 ng/ml of CK. The antibodies exhibited minor or even no cross reactivities with protopanaxatriol (1.56%) and other tested ginsenosides, $GRb_1$ (0.11%), $GRg_1$ (0.07%) except protopanaxadiol (87.2%) from the structural similarity. And the antibody showed good correlation (r=0.987) between the assay values obtained by this ELISA method and HPLC. Therefore, the ELISA method could be very useful tools for the determination of CK in biological fluids because of their high sensitivity and specificity.

Natural Dyeing of Silk Fabrics with Humulus japonicus Extract (환삼덩굴을 이용한 견직물의 천연염색에 관한 연구)

  • Ha, Young Kab;Lee, Jeong Sook
    • Textile Coloration and Finishing
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    • v.26 no.3
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    • pp.263-271
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    • 2014
  • The purpose of this study is to investigate the dyeability of silk fabrics with Humulus japonicus (Hwansam) extract which is a kind of weeds that grows wild in the suburbs. In the research, the general extract method was conducted with the extract from fresh herb of root-removed Humulus japonicus while we investigated the optimal dyeing conditions and dyeability depending on the changes of the kind of mordants, methods and temperature. The functional properties such as antibacterial activities, deodorization and UV protection were also reviewed. The ideal output, as a result, was shown at 100% of dye concentration, $90^{\circ}C$ of dyeing temperature and 40 minutes of dyeing time while in terms of dye uptake depending on the kind of mordants and methods, it was found that among four mordants of $SnCl_2$, $AlK(SO_4)_2$, $CuSO_4$, and $FeSO_4$ post-mordanting with $FeSO_4$ showed the best result. Color fastness to washing, dry cleaning and rubbing was found strong at grade 4-5 and the grade to light was as good as 3 while to perspiration was good at 4-5 as well. In aspects of functional properties, it showed excellent results of 99% deodorization rate, 98% UV protection rate and 99.9% bacterial reduction against Staphylococcus aureus.

Application of a Commercial Diff-Quick Kit for Fish Hematology (어류 혈액 염색에 Diff-Quick kit의 적용 가능성 검토)

  • Park, Seong-U;Kim, Dong-Wan;Choe, Min-Sun
    • Journal of fish pathology
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    • v.17 no.2
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    • pp.151-157
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    • 2004
  • Application of a commercial Diff-Quick kit for fish hematology was evaluated. Diff-Quick stain was incomplete in staining not only the nucleus and cytoplasm but also granules of leucocytes that were stained in soluton I and II for five seconds. However, when the staining time changed to 5 seconds in solution I and 10 seconds in solution II, followed by washing with deionized distilled water, the kit showed the similar results as in May-Grunwald Giemsa stain. The results indicate that the kit can be used in fish hematology for rapid staining.

Microbiological Hazard Analysis of Non-Heating Process Menus Served at Foodservice Operations and Hygienic Improvements by Implementing HACCP (급식소에서 제공되는 비가열조리 음식의 위해요인 분석과 HACCP 적용 후 위생개선효과)

  • Lee, Mi-Ra;Kim, Heh-Young
    • Korean journal of food and cookery science
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    • v.23 no.5
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    • pp.749-760
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    • 2007
  • The purpose of this study was to evaluate the microbiological quality of non-heat-processed foods and the effects of HACCP implementation. Here, cabbage salad and cucumber&onion salad were selected and we investigated HA(Hazard Analysis) by checking microbiological quality, time and temperature, pH, and water activity at each processing stage. Thus, the receiving of spices and dressings, washing and sterilizing, cutting, cooking, and serving stages were all considered CCPs. Before implementing HACCP, microbial analysis showed that standard plate counts and coliform counts were higher than standard levels in most of the raw ingredients of each menu, as well as during the production process. The microbiological quality of the utensils and employee's hands used during cooking indicated levels requiring direct management. Evaluations of falling bacteria-in the foodservice establishment work areas ranged from $2{\sim}12CFU/plate$. However, after HACCP implementation, microbiological levels improved to standard levels fly sanitation education. Also, the number of falling bacteria were lower than before implementing HACCP. Therefore, it is essential the foodservice operations make efforts to implement HACCP, so that microbiological hazard levels are lowered and hygienic status improved.

Feasibility Study for Dietitians' Roles as a Sanitation Consultant based on Managers' Self-evaluation on Sanitation Management at Restaurants in Cheonan (외식업소 경영자의 위생관리 평가분석을 통한 영양사의 지역내 일반음식점 위생교육 지도 필요성 고찰)

  • Lee, Jin-Mi;Lee, Jeong-Hui;Park, Jeong-Suk
    • Journal of the Korean Dietetic Association
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    • v.9 no.4
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    • pp.273-277
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    • 2003
  • The purpose of this study was to examine sanitation management practices of restaurant managers for the training needs analysis. A total of 26 restaurant managers participated in this study. A check list was consisted of three parts : facility, personal, and food hygiene. Two observers and one manager evaluated the same check list at same time and the results were compared. The results of this study suggested that most restaurants have needed for re-training programs in the view of facility, personal, food sanitation practices. Specially, proper washing and sanitizing methods for hands and utensils, proper cooking and holding temperature, and proper storing methods were needed to be trained. Based on this study, most independent restaurant managers in Cheonan were aware of training, but they had no effective training program manuals. Results of this study implicated that dietitians have new opportunity for consultants of independent restaurants in the region because they have practiced sanitation management manuals.

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The viewpoint-based product information modeling in collaborative product development (협업적 제품개발에서의 관점기반 제품정보 모델링)

  • 채희권;최영환;김광수
    • Proceedings of the CALSEC Conference
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    • 2003.09a
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    • pp.54-59
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    • 2003
  • The information sharing is essential to make collaboration by participants in the collaboration environment. The sharing of the information is necessary to reduce time-to-market of new Product. In this paper, V2-model is proposed far supporting the sharing of the information on product development. V2-model supports collaborative product development in design and supply chain. Through viewpoints, V2-model supports 1) two-level structure that consist of private level and public level ,2) level-up process and 3) product development process. The public level information supports to share the product information on collaborative supply chain and design. The viewpoints in V2-model are divided into public viewpoints that point to the public level information and private viewpoints that point to the private level information. Private viewpoints are transformed into public viewpoints. The extended Topic Map has B-Topic, S-Topic and View for representing V2-model in this paper. The level-up process of V2-model is implemented through the merging of S-Topics. V2-model is implemented with washing machine model using extended Topic Maps. In this model, the public viewpoints and private viewpoints are represented and the level-up process, which transforms private viewpoints into public viewpoints, is implemented.

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Dyeing of Flame Retardant Polyester Fabric developed by using Low-melting-point Bicomponent Filament (저융점 복합사를 이용한 난연 폴리에스터 직물의 염색)

  • Lee, Shin-Hee
    • Fashion & Textile Research Journal
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    • v.15 no.3
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    • pp.467-476
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    • 2013
  • This study investigates the dyeability and the fastness of flame retardant polyester fabric developed by a thermal bonding with a low melting component of flame retardant bicomponent filament (LMFRPC). The fabrics were prepared with flame retardant polyester filaments (FRP) as warp and blended filaments of FRP and LMFRPC as weft. The LMFRPC have a sheath and a core where the core comprises a flame retardant polyester and the sheath comprises a thermoplastic polyester with a low-melting point. The thermal bonding of fabric was conducted in a pin tenter at $170^{\circ}C$ for 60 seconds. Fabric dyeing was conducted with an infrared dyeing machine at various dyeing temperatures and dyeing times. The dyestuffs used in this study were CI disperse Yellow 54, Red 60 and Blue 56 of E-type dyestuff and Orange 30, Red 167 and Blue 79 of S-type dyestuff. This study investigated the morphology of thermal bonded fabric, dyeability and fastness of dyed fabric. Dyeability increased with an increased dyeing temperature. The thermal bonded area increased with the increased LMFRPC content. The dyeability of S-type dyestuff was higher than E-type dyestuff; in addition, the saturated dyeing time was about 20minutes at $130^{\circ}C$ for E and S-type dyestuff. The fastness to washing and rubbing were excellent at a 4-5 Grade.

Study of the Clothing Behavior and Fiber Preferences of Multicultural Families (다문화 가정의 의복 실태와 섬유 선호도에 관한 연구)

  • Choi, In-Ryu
    • The Research Journal of the Costume Culture
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    • v.19 no.4
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    • pp.863-875
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    • 2011
  • The purpose of this study was to survey the clothing behavior, care and the fiber preferences of multi-cultural families living in Korea. The data was collected using questionnaire surveys based on pre-tests, and a main survey conducted in Seoul, Gyeonggi Province and Chungcheong Province. The 258 participants came from 151 multi-cultural families and 107 Korean families. The results of this study were as follows: First, Filipinos owned more clothes than the Chinese and bought clothes more frequently. The Filipinos spent 10,000~30,000 Korean won on all types of clothing. Each time, the Chinese spent more money when purchasing jackets. Second, when health was the greatest concern for underclothes, they chose cotton fiber as their preferred fiber. When beauty was of greatest concern for blouses they chose cotton. When beauty was of greatest concern for skirts and jackets they chose natural fibers such as silk, wool or linen. Filipinos preferred synthetic fibers because they are easy to care for, and the Chinese preferred natural fibers due to their beauty, especially for pants. Third, multi-cultural families laundered at home using only a washing machine. All fibers were laundered together into the washer without any sorting. This study can contribute to providing basic data for an understanding of the clothing behavior and laundry styles of multi-cultural families which may be useful data in the apparel market in Korea given the relative and direct changes relevant to various clothing cultures.