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The measurement of dicamba in soil and plants (토양 및 식물 중 디캄바 측정법에 대한 연구)

  • Shin, Ho-Sang
    • Analytical Science and Technology
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    • v.22 no.6
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    • pp.480-487
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    • 2009
  • The herbicide dicamba (2-methoxy-3,6-dichlorobenzoic acid) in soil and plants was determined by gas chromatography-mass spectrometry (GC/MS). The samples were extracted with diethyl ether at pH 2, and washed with 0.1 N HCl, and then dried. The dried residue was derivatized in 1 mL of 10% $H_2SO_4$-MeOH for 2 hr at $80^{\circ}C$. The reaction mixture was neutralized with 4 mL of sodium bicarbonate solution and reextracted with 5 mL of diethyl ether. After the extract was concentrated, dicamba was determined by GC/MS-SIM mode. There was good linearity above 0.999 in the ranges of the $1.0{\sim}100{\mu}g/kg$. Total 42 sample including 32 soil samples and 10 plants samples were analyzed by developed method. Dicamba was detected in the concentration range of $2.9-123.9{\mu}g/kg$ in 15 samples among 32 soil samples and in the concentration range of $43-33,252{\mu}g/kg$ in 5 samples among 10 plants samples. A cause of the wither and die of the pine trees is suspected to spray dicamba around or directly to them.

Estimating carbon uptake in forest and agricultural ecosystems of Korea and other countries using eddy covariance flux data (에디 공분산 기반의 플럭스 타워 관측자료를 이용한 국내외 산림과 농업 생태계 탄소 흡수량 분석)

  • Lee, Bora;Kang, Wanmo;Kim, Choong-Ki;Kim, Gieun;Lee, Chang-Hoon
    • Journal of Environmental Impact Assessment
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    • v.26 no.2
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    • pp.127-139
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    • 2017
  • Measurements of net ecosystem exchange (NEE) of $CO_2$ based on the eddy covariance technique provide reasonable carbon balance estimates in response to local environmental conditions. In South Korea, the forest ecosystems cover approximately 64% of the total area, thereby strongly affecting regional carbon balances. Cultivated croplands that cover about 17% of the total area should also be considered when calculating the carbon balance of the country. In this study, our objectives were (a) to quantify the range and seasonal variation of NEE at forest ecosystems, including deciduous, coniferous, and mixed forests, and agricultural ecosystems, including rice paddies and a potato field, in South Korea and (b) to compare NEE at ten Fluxnet sites that have the same or similar ecosystems as found in South Korea. The results showed that the forest and agricultural ecosystems were carbon sinks. In Korea, NEE at the forest ecosystems varied between -31 and $-362gC/m^2/yr$, and NEE at the croplands ranged from -210 to $-248gC/m^2/growing$ season. At the deciduous forest, NEE reached low values in late spring, early summer, and early autumn, while at the coniferous forest, it reached low values in spring, early summer, and mid autumn. The young mixed forest was a much stronger carbon sink than the old-growth deciduous and coniferous forests. During each crop growing season, beet had the lowest NEE value within six crops, followed by wither wheat, maize, rice, potato, and soybean. These results will be useful for designing and applying management strategies for the reduction of $CO_2$ emissions.

Physiological Responses to Drought Stress of Seven Evergreen Hardwood Species (상록활엽수 7수종의 건조스트레스에 대한 생리적 반응)

  • Jin, Eon-Ju;Cho, Min-Gi;Bae, Eun-Ji;Park, Junhyeong;Lee, Kwang-Soo;Choi, Myung Suk
    • Journal of Korean Society of Forest Science
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    • v.106 no.4
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    • pp.397-407
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    • 2017
  • This research aims to analyze and compare the drought resistance of 7 species of landscape trees commonly grown in Korea. The 7 species are: Camellia japonica, Rhaphiolepis indica, Quercus glauca, Machilus thunbergii, Daphniphyllum macropodum, Dendropanax morbifera and Cinnamomum camphora. In order to analyze their drought resistance, the samples were left without irrigation for 30 days (05/09/2016 ~ 05/10/2016), during which period their respective drought resistor, relative water content, electrolyte elution figures and proline content were measured. As the non-irrigation proceeded, C. camphora was the first to wither, followed by D. morbifera, then D. macropodum, then M. thunbergii, then Q. glauca, then R. indica then finally C. japonica. Of the 7 species, Q. glauca, C. japonica and R. indica can be considered highly drought resistant, since they survived for longer than 3 weeks without irrigation. Relative water content (RWC) plummeted dramatically after the first 15 days of non-irrigation. Whereas RWC readings of C. camphora, D. morbifera, D. macropodum and M. tunbergii dropped by 40% or more, the other 4 species reported a relatively low rate of decrease at 20% or lower. The Camellia japonica, the R. indica and Q. glauca, which were the species with relatively high drought resistance, showed low proline content and electrolyte elution figures, whereas those of C. camphora, D. macropodum, D. morbifera and M. tunbergii were higher. Analysis through the nonlinear regression analysis logistic model showed that non-irrigation proved fatal for the 7 sample species in a range of 22.7 to 37.6 days. The C. japonica, R. indica, Q. glauca and M. tunbergii demonstrated a high drought resistance of 30 days or longer, whereas C. camphora, D. morbifera and D. macropodum had a low resistance of 25 days or less to drought from lack of water. In conclusion, out of the 7 species of broad-leaved evergreen trees tested, C. japonica, R. indica and Q. glauca seem to be suitable for use as landscape trees, owing to their high drought resistance.

Molecular cloning and characterization of β-1,3-glucanase gene from Zoysia japonica steud (들잔디로부터 β-1,3-glucanase 유전자의 클로닝 및 특성분석)

  • Kang, So-Mi;Kang, Hong-Gyu;Sun, Hyeon-Jin;Yang, Dae-Hwa;Kwon, Yong-Ik;Ko, Suk-Min;Lee, Hyo-Yeon
    • Journal of Plant Biotechnology
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    • v.43 no.4
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    • pp.450-456
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    • 2016
  • Rhizoctonia leaf blight (large patch) has become a serious problem in Korean lawn grass, which is extremely hard to treat and develops mostly from the roots of lawn grass to wither it away. Rhizoctonia leaf blight (large patch) is caused by Rhizoctonia solani AG2-2 (IV). To develop zoysia japonica with strong disease tolerance against this pathogenic bacterium, ${\beta}-1,3-glucanase$ was cloned from zoysia japonica, which is one of the PR-Proteins known to play a critical role in plant defense reaction. ${\beta}-1,3-glucanase$ is known to be generated within the cells when plant tissues have a hypersensitive reaction due to virus or bacterium infection and secreted outside the cells to play mainly the function of resistance against pathogenic bacteria in the space between the cells. This study utilized the commonly preserved part in the sequence of corn, wheat, barley, and rice which had been researched for their disease tolerance among the ${\beta}-1,3-glucanase$ monocotyledonous plants. Based on the part, degenerate PCR was performed to find out the sequence and full-length cDNA was cloned. E.coli over-expression was conducted in this study to mass purify target protein and implement in vitro activation measurement and antibacterial test. In addition, to interpret the functions of ZjGlu1 gene, each gene-incorporating plant transformation vectors were produced to make lawn grass transformant. Based on ZjGlu1 protein, antibacterial activity test was conducted on 9 strains. As a result, R. cerealis, F. culmorum, R.solani AG-1 (1B), and T. atroviride were found to have antibacterial activity. The gene-specific expression amount in each organ showed no huge difference in the organs based upon the transformant and against 18s gene expression amount.

Studies on the Cause and Control for Non-sprouting Bud in the Utility Reclaimed Hill Side Mulberry Field. (개간지 상전에 발생하는 발아불량 현상의 원인 및 방제에 관한 연구)

  • 유근섭
    • Journal of Sericultural and Entomological Science
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    • v.16 no.1
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    • pp.1-20
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    • 1974
  • These studies are conducted to find out inducing factors for the non-sprouting bud which appears in the utility reclaimed hill mulberry held and to establish control method for it since 1971. The results are as follows: 1. In spring, winter bud does not germinate at all on the top of branches or does wither suddenly after or during its germination. Necrosis and browning are appeared in cortex and phloem of non-sprouting bud branch. In autumn, the deterioration of leaves, the abnormal leaves on the top of branch, and browning of veins or petioles are also observed. 2. The soil of non-sprouting bud mulberry Held were found to be sand loom derived from granite rocks. The sub soil of it was very poor in development of root system because it had hard soil and lower pore space ratio. Especially, the sub soil to bring about severe non-sprouting bud had been easily appeared with deficiency of moisture content because of high density solid. 3. Content of soil moisture was significantly lower in the severe non-sprouting bud soil than in the healthy field. The sub soil o( the healthy field contained proper moisture content. On the other hand, the sub soil of the severe non-sprouting bud held contained almost critical moisture content for wilting. 4. The depth of available soil was shallow in the non-sprouting bud than in the healthy fold. The more rate of the non-sprouting bud was severe, the more available soil depth was sallow. 5. Available boron content in soil was affected by moisture content in soil. There was. lower moisture content in the non-sprouting bud field than that in healthy fold during 5, June to 5, September. 6. There was no significant correlation between soil pH and available boron content. On the other hand, the correlation of the content of organic matter and available boron content appeared to be highly positive significance. 7. The quantity of boron was significantly contained more in healthy mulberry field (0.34∼0.43ppm) than in non-sprouting bud field (0.10∼0.28ppm). 8. Boron content in leaves and barks was significantly lower in the non-sprouting bud trees than in healthy trees. 9. The symptom of non-sprouting bud induced from boron free sand culture was similar with that arised in the non-sprouting bud field. 10. The rate of non-sprouting bud was high by the increased application of lime. Considering the facts mentioned the above, author may conclude that the non-sprouting bud of mulberry tree is caused by boron deficiency, but also it is affected by the parent rocks, organic matter, soil moisture content and lime application. 11. The non$.$sprouting bud may be completely controlled by the application of 6∼9kg borax per 10a mulberry field twice a year in spring and summer.

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Estimation of Primal Cuts Yields by Using Body Size Traits in Hanwoo Steer (한우 후대검정우의 체척형질을 통한 부분육 생산량 추정)

  • Lee, Jae Gu;Lee, Seung Soo;Cho, Kwang Hyun;Cho, Chungil;Choy, Yun Ho;Choi, Jae Gwan;Park, Byoungho;Na, Chong Sam;Roh, Seung Hee;Do, Changhee;Choi, Taejeong
    • Journal of Animal Science and Technology
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    • v.55 no.5
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    • pp.373-380
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    • 2013
  • The study aimed to develop prediction models of primal cut yield using body measurements of Hanwoo steers in Korea. The progeny of 874 steers at Hanwoo Improvement Main Center from 2008 to 2010 were recorded. Pearson's correlation coefficients for primal cuts and other traits were estimated. Primal cuts were adjusted for slaughter date and age using the SAS GLM procedure. Afterwards, a stepwise regression was performed on each primal cut by fitting body measurement traits. An independent covariable was selected at the highest coefficient of determination with the greater fitness model using Mallows's Cp statistic. Results showed that primal cuts were significantly influenced by slaughter date (P<0.01). The age at slaughter, however, was only significant for the top round (P<0.05). There was a moderate to high correlation between chest girth and tenderloin (0.54), loin (0.74), and rib (0.80). Most primal cut percentages were negatively related to BFT. Similar negative to low positive correlations were observed for primal cut percentage and body size traits. In addition, a correlation of 0.21 was observed between rib percentage and chest girth. The regression of body measurements on the adjusted primal cuts were significant for later traits. Regression estimates revealed that wither height, body length, rump length, hip bone width, and chest girth are important for primal cut weight and percentage determination. In particular, chest girth was always important for primal cut weight estimates.

Geographic Conditions and Garden Designs of Byeol-seo Scenic Site of Gimcheon Bangcho-Pavilion and Mrs Choi's Pond (별서 명승 김천 방초정(芳草亭)과 최씨담(崔氏潭)의 입지 및 조영 특성)

  • Rho, Jae-Hyun;Lee, Hyun-Woo
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.34 no.1
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    • pp.71-82
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    • 2016
  • Through literature review and on-site survey of Gimcheon Bangcho pavilion(芳草亭), the features of garden design(庭園意匠) including geographic conditions, landscape modeling of Nujeong(樓亭) and Jidang(池塘, Pond), and scenic interpretations in Nujeong Jeiyoung poetry(樓亭題詠詩) have been carefully researched and the findings are presented below. Bangcho pavilion is located in a village called Wonteomaeul, which belongs to the feng shui state of golden hairpin and floating lotus. It has long been the cultural hub of communication and social interactions among the villagers. The Head House of Jeongyanggong(靖襄公宗宅), the main house(本第) of the Yeonan Yi Clan(延安李氏), is about 150m away from Bangcho pavilion, an artistic space whose landscape modeling is of the form called Nujeong. The name 'Bangcho' reflects the noble man(君子)'s determination: "I yearn for the place where honey parrots fly and the fragrant grass grow." From the two story structure of the pavilion where there is an additional floor installed to the central ondol room by a four-sided subdivision door, one can detect the aspiration of the men for pursuing an open view. One can also observe the efforts in designing the room to be used for multiple purposes from a private place to an office for periodic publication of a family lineage document called "Garyejunghae(家禮增解)". Bangcho pavilion's main sight of interest is Mrs Choi's Pond(崔氏潭), the one and only garden structure that comprises the twin round island of square pond(方池雙圓島) among the existing Jidangs in Korea. In this special Jidang, there are two coexisting islands that represent a well thought out garden facility for symbolizing conjugal affection and unyielding fidelity between master and servent(主從). In addition, the three inflows and one outflow facing the Ramcheon valley is regarded as an ideal garden design optimized for performing the function of a village bangjuk which is the site for undercurrent creation and ecological reprocessing. At present, Giant pussy willow is the only circular vegetation identified in the area of Bangcho pavilion, although this plant species is about to wither away judging from the signs of decrepitude that seems to persist for two out of three weeks. The old pine tree that appears in the 1872 Jeiyoung poetry of Byeongseon Song(宋秉璿) no longer exists. Anjae(安齋) Jang Yoo(張瑠)'s "Eight Scenary on Bangcho pavilion(芳草亭八詠)" and its expansive reproduction "Ten Scenary on Bangcho pavilion(芳草亭十景)" from Gwagang(守岡) Lee Manyoung(李晩永) depict vividly the pastoric scenery of an idyll(田園景) that stretches throughout the natural and cultural landscape of the province of Gimcheon and Guseong surrounding the Bangcho pavilion. The Bangcho pavilion sutra aims to establish Bangcho pavilion and the village of Wonteomaeul as the centre of microcosmos by dividing and allocating its scenic features according to the four seasons and times(四季四時), the eight courses(八方) and the meteorological phenomena, and it is the incarnation(顯現) of landscape perception upon the Byeol-seo Scenic site of Bangcho pavilion, the cultural hub of the region.

Effects of Genetic and Environmental Factors on Average Daily Gain, Feed Efficiency and Body Measurements in Korean Native Bull (한우(韓牛)의 일당증체량(日當增體量), 사료이용성(飼料利用性), 체중(體重)과 체척측정치(體尺測定値)에 미치는 유전(遺傳) 및 환경효과(環境效果))

  • Sang, Byoung Chan;Han, Sung Wook;Kang, Heung Joo
    • Korean Journal of Agricultural Science
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    • v.16 no.1
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    • pp.62-70
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    • 1989
  • This study was conducted to determine the main effects of the sire, farrowing year, month and parity on the average daily gain, feed efficiency, body weight and body type on the basis of data obtained from 64 Korean native bulls from 8 sires raised at Chungnam Animal Breeding Station from 1985 to 1987. The results obtained are summarized as follows; 1. Average daily gain, DCP and TDN requirement were 1.049, 0.977 and 4.584kg, and the effect of sire was highly significant(p<0.01) for average daily gain and TDN requirement, respectively. 2. Body weight at 6 and 12 months of age were 182.023 and 371.750kg, and the effects of sire, farrowing year and month were highly significant(p<0.01) for body weights, respectively. 3. Wither height and body length were 106.672 and 108.986cm for 6 months of age, 119.984 and 132.312cm for 12 month of age, and the effect of sire was significant(p<0.05) for these all traits, respectively. 4. Chest girth and chest width were 129.578 and 27.937cm for 6 months of age, 166.281 and 38.357cm for 12 months of age, and the effect of sire was significant(p<0.05) for chest girth at 6 and 12 months of age, respectively.

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Increase of Spoken Number of Syllables Using MIT(Melody Intonation Therapy) : Case Studies on older adult with stroke and aphasia (MIT(Melodic Intonation Therapy) 중심의 음악활동을 이용한 실어증을 가진 뇌졸중 노인의 음절 수 증가에 대한 사례 연구)

  • Hong, Do Kyoung
    • Journal of Music and Human Behavior
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    • v.2 no.2
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    • pp.57-67
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    • 2005
  • Most of stroke patients have not only physical difficulty but speech and neurological disorder because of hemiplegia and such unexpected changes cause psychologic disadaptability and absent-mindedness. Particularly, lowering of physical ability can lead to serious emotional problem from failure or frustration in daily life. Generally, treatment of patient with stroke put emphasis on physical rehabilitation but actually this patient had considerable speech disorder such as aphasia or articulation disorder. Moreover, failing of recognition function, mental disorder as hypochondria, and even visual and auditory disorder are represented. So it is effective to integrate verbal remediation and other treatments in medical care environment. In particular, patients with language disorder very often wither psychologically therefore it is efficient to use of music therapy that gives opulent emotion to aphasia patients. And primarily to investigate the effects of 10 sessions treatments; change in spoken total number of syllables, to confirm their own value by success of given task and reassure about themselves ability. All of 10 sessions stages were scored by MIT manual and its improvement were measured, that is, accomplishment was analyzed within each level in order to prove detail change of spoken total number of syllables. The result of this program organized from 2 syllables to 4 syllables is summarized as follows. Subject A completed in preliminary stage Level I, in 2 syllables case advanced to Level III in fifth session and to Level IV in seventh session, in 3 syllables case advanced to Level III in seventh session and to Level IV in ninth session, and in 4 syllables case showed 8% low success rate in first session but after repeated practice increased considerably in sixth session and in advanced to Level III in eighth session to Level IV in tenth session. Subject B also completed in preliminary stage Level I, in 2 syllables case advanced to Level III in forth session and to Level IV in sixth session, in 3 syllables case advanced to Level III in fifth session and to Level IV in seventh session, and in 4 syllables case showed 10% low success rate in first session and increased considerably in fifth session and in advanced to Level III in seventh session but could not reach to Level IV until tenth session. As a result, it was shown that music therapy using MIT was not statistically meaningful but improved spoken total number of syllables and success rate of task had improved as a whole. Therefore, music intervention using MIT it has positive affect on verbal ability of patients with Broca's Aphasia and their language rehabilitation.

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The Nature Cognition and Lyricism Reflected in the Pavilion Tablets - Focusing on the Pavilions in the Damyang Poetry Culture Area Built during in 16th Century - (누정편액(樓亭扁額)에 반영된 자연인식과 서정 - 16세기 담양 시가문화권의 누정을 중심으로 -)

  • Lee, Hyun-Woo;Kim, Jai-Sik
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.28 no.3
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    • pp.1-13
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    • 2010
  • This research is the semantic study on the nature cognition and lyricism of pavilion builders as reflected in the pavilion tablets in the Damyang poetry culture area built during 16th century. The names of pavilions, pavilion writings and "poetry with given theme" were studied by actual site visits. The view on the nature and aesthetic awareness of the pavilion builders at the time were explored together with surrounding landscapes. Following conclusions were drawn. 1. Pavilion names borrowed phrases from the famous historical events left by the sages. Or, they took the compliment on the beautiful nature surrounding orthe academic achievement of pavilion owners as the theme, then elaborated them with thinking based on Sung Confucianism. 2. The writings of pavilion literature were the idealistic view on the nature. They think the nature and human as "object and ego are one, object and ego communicate each other, object and ego become one". 3. The nature in the 16th century was the "principle of birth-death-grow-wither" for the intellectuals. They thought that the nature is the ultimate place to return for the realization of life with self-respect. 4. The introduction of fairy land among literary activities in the pavilion was the symbolic means healing the frustrated ego. It was confirmed that they temporarily entrusted their depressed feelings by borrowing the fairy land, far from the reality. The pavilion was the space to escape from the reality and soothe the anger and discord in the reality. 5. The pavilion literature in the Damyang poetry culture area during 16th century sang their pleasure of enjoying the beautiful scenery in the rural area using their social status. The literary works also contained their agony due to the frustration of difficulty in the realization of ideal. When the intellectuals, who already mastered literature, history and philosophy, came down to rural area, built pavilions at the location with beautiful scenery, and spent time while composing poetry, it was an escape from the burden that they should take the social responsibility as the model intellectuals of the society. The pavilions in Damyang poetry-culture area during 16th century were the idealized conceptual world and the space of awareness reflecting the philosophy and thinking of the time.