• Title/Summary/Keyword: W/C ratio

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Optimization of Betacyanin Production by Red Beet (Beta vulgaris L.) Hairy Root Cultures. (Red Beet의 모상근 배양을 이용한 천연색소인 Betacyanin 생산의 최적화)

  • Kim, Sun-Hee;Kim, Sung-Hoon;Lee, Jo-No;An, Sang-Wook;Kim, Kwang-Soo;Hwnag, Baik;Lee, Hyeong-Yong
    • Microbiology and Biotechnology Letters
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    • v.26 no.5
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    • pp.435-441
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    • 1998
  • Optimal conditions for the production of natural color, betacyanin were investigated by varying light intensity, C/N ratio, concentrations of phosphate and kinds of elicitors. Batch cultivation was employed to characterize cell growth and betacyanin production of 32 days. The maximum specific growth rate, ${\mu}$$\sub$max/, was 0.3 (1/day) for batch cultivation. The maximum specific production rate, q$\^$max/$\sub$p/, was enhanced 0.11 (mg/g-cell/day) at 3 klux. A light intensity of 3 klux was shown to the best for both cell growth and betacyanin production. The maximum specific production rate was 0.125 (mg/g-cell/day) at 0.242 (1/day), the maximum specific growth rate. The dependence of specific growth rate on the light lintensity is fit to the photoinhibition model. The correlation between ${\mu}$ and q$\sub$p/ showed that the product formation parameters, ${\alpha}$ and ${\beta}$$\sub$p/ were 0.3756 (mg/cell) and 0.001 (mg/g-cell/day), respectively. The betacyanin production was partially cell growth related process, which is different from the production of a typical product in plant cell cultures. In C/N ratio experiment, high carbon concentration, 42.1 (w/w) improved cell growth rate while lower concentration, 31.6 (w/w) increased the betacyanin production rate. The ${\mu}$$\sub$max/ and q$\^$max/$\sub$p/ were 0.26 (1/day) and 0.075 (mg/g-cell/day), respectively. Beta vulgaris L. cells under 1.25 mM phosphate concentration produced 10.15 mg/L betacyanin with 13.46 (g-dry wt./L) of maximum cell density. The production of betacyanin was elongated by adding 0.1 ${\mu}$M of kinetin. This also increased the cell growth. Optimum culture conditions of light intensity, C/N, phosphate concentration were obtained as 5.5 klux, 27 (w/w), 1.25 mM, respectively by the response surface methodology. The maximum cell density, X$\sub$max/, and maximum production, P$\sub$max/, in optimized conditions were 16 (g-dry wt./L), 12.5 (mg/L) which were higher than 8 (g-dry wt./L), 4.48 (mg/L) in normal conditions. The ${\mu}$$\sub$max/ and q$\^$max/$\sub$p/ were 0.376 (1/day) and 0.134 (mg/g-cell/day) at the optimal condition. The overall results may be useful in scaling up hairy root cell culture system for commercial production of betacyanin.

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LCD contrast ratio enhancement method using Carbon Nanotube Back Light Unit and Local Dimming (CNT-BLU Local Dimming 구동을 이용한 LCD Contrast 향상 방법)

  • Min, K.W.;Chung, D.S.;Song, B.G.;Kim, S.L.;Kang, H.S.;Baik, C.W.;Jeong, T.W.;Kim, J.W.;Jin, Y.W.;Cho, J.D.
    • Proceedings of the IEEK Conference
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    • 2006.06a
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    • pp.971-972
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    • 2006
  • We have demonstrated Carbon Nanotube Back Light Unit (CNT-BLU) which has a triode structure. Local dimming scheme was introduced to the BLU driving system. With this driving method, contrast ratio enhanced 20 times higher than that of conventional Cold Cathode Fluorescent Lamp (CCFL) BLU.

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The Performance & Operation Analysis of a Steam Reformer for MCFC (MCFC용 Steam Reformer 개질성능 분석 및 운전평가)

  • Seo, Hai-Kung;Koh, Joon-Ho;Lim, Hee-Chun
    • Transactions of the Korean hydrogen and new energy society
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    • v.11 no.4
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    • pp.149-159
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    • 2000
  • This paper is concerned with the performence of a steam reformer for 25kW class MCFC, which is compared with the theoretically calculated results at various operating conditions. The theoretical $H_2$ production amount and $CH_4$ conversion rate are calculated with variations of temperature and steam/carbon (S/C) ratio using fortran program, and the actual values are measured from flowmeter and gas chromatography. As a result of the comparison of theoretical and actual values, the theoretical $H_2$ production amount is calculated by $24.4m^3/hr$ at the normal operating condition(LNG $9m^3/hr$, S/C ratio 5, absolute pressure $2.77kg/cm^2$, $610^{\circ}C$), but the actual production amount is only $19.4m^3/hr$, which is 79.5% of the theoretical value. Nevertheless, at the normal operating condition, the reformer for 25kW class MCFC performed well for a 2,100 hr long run operation, constantly producing $H_2$.

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The Dry Sliding Wear Properties of $SiC_w$ and $SiC_p$ Reinforced Bronze Matrix Composites (무윤활 미끄럼 마찰하에서 SiC 휘스커 및 입자강화 청동기지 복합재의 마모특성)

  • 이상로;허무영
    • Tribology and Lubricants
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    • v.9 no.2
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    • pp.49-55
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    • 1993
  • The dry sliding wear properties of the sintered Cu-10 wt%Sn bronze alloys reinforced with $SiC_w$ and $SiC_p$ were investigated by a pin-on-disc wear testing machine. The worn surfaces and the cross sections of the wear specimens and the wear debris were observed by SEM to study the effect of the variation of the ceramic phase contents in the composite and the wear condition on the wear behaviors. The wear of bronze matrix was dominated by the adhesive wear. The transition from mild to severe wear was found in the bronze matrix specimens at the applied load higher than 20N where the surface delamination caused the severe wear. The addition of $SiC_w$ and $SiC_p$ reinforcements in the romposites was proved to reduce the wear rate by the matrix strengthening at the applied load higher than 20N. SiC whiskers having a large length to diameter ratio which hold the deformed matrix were effective to hinder the crack propagation near the worn surface. Thus the maximum wear resistance was obtained in the composite reinforced by $SiC_w$ at the higher applied load.

Statistical flexural toughness modeling of ultra high performance concrete using response surface method

  • Mosabepranah, Mohammad A.;Eren, Ozgur
    • Computers and Concrete
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    • v.17 no.4
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    • pp.477-488
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    • 2016
  • This paper aims to model the effects of five different variables which includes: cement content (C), the steel fiber amount (F), the silica fume amount (SF), the superplasticizer (SP), the silica fume amount (SF), and the water to cementitious ratio (w/c) on 28 days flexural toughness of Ultra High Performance Concrete (UHPC) as well as, a study on the variable interactions and correlations by using analyze of variance (ANOVA) and response surface methodology (RSM). The variables were compared by fine aggregate mass. The model will be valid for the mixes with 0.18 to 0.32 w/c ratio, 4 to 8 percent steel fiber, 7 to 13 percent cement, 15 to 30 percent silica fume, and 4 to 8 percent superplasticizer by fine aggregate mass.

Manufacture technology of tofu shake added with red rice (홍국쌀을 첨가한 두부 셰이크의 제조기술)

  • Cho, Eun-Jeong;Lee, Sang-Soo;Kwon, Dong-Jin
    • Food Science and Preservation
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    • v.24 no.7
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    • pp.942-948
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    • 2017
  • To establish manufacturing technology of tofu shake added with red rice prepared with Monascus purpureus KCCM 60570, the quality characteristics of tofu shake added with M. purpureus KCCM 60570 were investigated. Also, we determined the optimal mixing ratios of raw materials including red rice for manufacturing tofu shake. Monacolin K and mold number of red rice were increased rapidly until $8^{th}$ day of incubation and then gradually increased during the 12 day manufacturing period. As the results, it was considered to be most suitable to prepare red rice for 8 days at $25^{\circ}C$, and inoculate 1% (w/w) M. purpureus KCCM 60570. Sensory evaluation of tofu shake added with red rice and tofu shake without red rice was not significantly different at p<0.05. In sensory evaluation, tofu shake added with 0.5% (w/w) red rice was higher in taste, texture, and overall acceptability than the other. Therefore, the optimal mixing ratio of red rice and tofu shake is 0.5% (w/w). These results suggest that the addition of red rice could improve the quality of tofu shake.

Dynamic Vibration Absorber Having Coil Springs and Oil Damper for a Damped Vibration System (감쇠진동계에 부착된 코일스프링과 오일댐퍼로 구성된 동흡진기)

  • Ahn, C.W.;Park, S.C.;Lee, H.B.
    • Journal of the Korean Society for Precision Engineering
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    • v.13 no.12
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    • pp.129-135
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    • 1996
  • This paper presents the effectiveness of the dynamic vibration absorber consisting of a single mass, coil springs and oil damper on the resonance freauency ratio and amplitude ratio for damped linear systems, that is, primary vibration system with damping. The effects of the dynamic vibration absorber are investigated numerically and experimentally for values of mass ratio, natural frequency ratio, and damping ratio. The experimental results show good agreement with calculated ones. As a result, the characteristics shown by the present work are useful in optimal tuning the dynamic vibration absorber in practice.

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Development of a Rice Circulating Concurrent-flow Dryer(III) - Performance Evaluation of 12-ton Capacity Dryer - (순환식 병류형 곡물건조기 개발(III) - 12톤 용량 건조기 성능평가 -)

  • Han, Jae-Woong
    • Journal of Biosystems Engineering
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    • v.34 no.5
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    • pp.358-362
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    • 2009
  • This study was conducted to evaluate the performance of a 12 tons capacity circulating concurrent flow rice dryer. An actual scale dryer with the capacity of 12 tons was developed to obtain a faster drying rate of 0.8~1.2%(w.b.)/h, while maintaining a lower drying energy consumption of 5,000 kJ/kg-water and achieving a drying quality that was comparable to a conventional cross-flow rice dryer. The Test-1 was conducted at $110^{\circ}C\;-\;20\;cmm/m^2$ and the Test-2 was conducted at $120-110-100-90^{\circ}C\;-\;20\;cmm/m^2$ under the same conditions as Test-1. In Test-1, the drying rate, drying energy consumption and crack ratio were 0.98 %(w.b.)/h, 4,573 kJ/kg-water and 3.2%, respectively. In Test-2, the drying rate, drying energy consumption and crack ratio were 0.74 %(w.b.)/h, 4,790 kJ/kg-water and 4.0%, respectively. The results of these tests demonstrated that this concurrent-flow dryer reached the desired drying rate, drying energy consumption and crack ratio.

A Two-stage Process for Increasing the Yield of Prebiotic-rich Extract from Pinus densiflora

  • Jung, Ji Young;Yang, Jae-Kyung
    • Journal of the Korean Wood Science and Technology
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    • v.46 no.4
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    • pp.380-392
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    • 2018
  • The importance of polysaccharides is increasing globally due to their role as a significant source of dietary prebiotics in the human diet. In the present study, in order to maximize the yield of crude polysaccharides from Pinus densiflora, response surface methodology (RSM) was used to optimize a two-stage extraction process consisting of steam explosion and water extraction. Three independent main variables, namely, the severity factor (Ro) for the steam explosion process, the water extraction temperature ($^{\circ}C$), and the ratio of water to raw material (v/w), were studied with respect to prebiotic sugar content. A Box-Behnken design was created on the basis of the results of these single-factor tests. The experimental data were fitted to a second-order polynomial equation for multiple regression analysis and examined using the appropriate statistical methods. The data showed that both the severity factor (Ro) and the ratio of water to material (v/w) had significant effects on the prebiotic sugar content. The optimal conditions for the two-stage process were as follows: a severity factor (Ro) of 3.86, a water extraction temperature of $89.66^{\circ}C$, and a ratio of water to material (v/w) of 39.20. Under these conditions, the prebiotic sugar content in the extract was 332.45 mg/g.